Isolation and characterization of phytokinin-binding proteins from the cotton plant

A highly purified site of a cytokinin-binding protein with a K_D value for³H-BAP of 4.3 nM has been isolated from cotton-plant seedlings by the methods of biospecific and hydrophobic chromatography. According to the results of electrophoretic analysis under denaturing conditions its molecular mass is 43 kDa.A. S. Sadykov Institute of Bioorganic Chemistry, Uzbekestan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 702–706, November–December, 1992.     

Phosphorylation of cottonplant proteins under the action of protein kinase inhibitors and activators

The occurrence of Ca²⁺-, Ca²⁺-phospholipid-, Ca²⁺-calmodulin-, and cAMP-dependent phosphorylation has been shown with the aid of protein kinase activators and inhibitors and by electrophoresis and autoradiography. Specific substrates have been revealed and it has been demonstrated that the pathways of the realization of the action of cAMP-dependent and Ca²⁺-calmodulin-dependent protein kinases may intersect.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 627071. Institute of Nuclear Physics, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 442603. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 96–100, January–February, 1994.     

Structure of acoseptine—A representative of a new type of norditerpenoid alkaloids

A new diterpenoid alkaloid, which has been called acoseptine, has been isolated from the plantAcontium septentrionale Koelle. This is the first alkaloid of the anhydrolycoctonine type with a C₈–C₁₇ bond and a carbonyl group at C₇. The structure of acoseptine has been established on the basis of IR, mass, and¹H and¹³C NMR spectra.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (371) 120 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 113–115. January–February, 1999.     

Protein kinase from cotton seeds

A Ca²⁺-dependent protein kinase C, active at pH 6.5–8.0 has been found in cotton seeds for the first time. The localization of the enzyme in the seeds has been established and some of its properties are described (stability in various media, capacity for performing the phosphorylation of various substrates, activation by calcium ions). Highly active preparations of cottonseed protein kinase C have been isolated by biospecific chromatography.Institute of Bioorganic Chemistry, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 255–259, March–April, 1989.     

VI. The structure of donine

A new dimeric alkaloid donine with the composition C₁₉H₂₂N₂O₄ has been isolated from the epigeal part of Arundo donax. On the basis of chemical transformations and UV, IR, mass, PMR, and¹³C NMR spectra the structure of diethyl NN-methylenebis(4-aminobenzoate) is proposed for this alkaloid.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent. fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii. No. 2, pp. 221–224, March–April, 1996. Original article submitted October 2, 1995.     

Influence of the glycoproteins of the cotton plant on the biosynthesis of proteins and nucleic acids

An inhibitory action of ELPs and LLPs on the inclusion of³H-thymine and³⁵S-methionine in the cells of a suspension culture and isolated nuclei of cottonplant seedlings has been found. The influence of the ELPs on the proliferative activity of cottonplant cells and of the ELPs and LLPs at the level of protein biosynthesis in isolated nuclei bears a dose-dependent nature, in contrast to the galactose-specific fraction LLP-1.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 62 70 71. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 865–870, November–December, 1997.     

Polydispersity of pectins and some physicochemical properties of their solutions

Pectin solutions obtained in the presence of HNO₃, HCl, H₂SO₄, and H₃PO₄ have been studied by viscometry, turbidity spectra, and ultracentrifugation. Cotton pectin extracts with phosphoric acid proved to be the most unstable. The molecular masses of pectins obtained from various materials (apple, lemon, and grape press residues, beet pulp, and cotton boll valves) ranged between 14,000 and 37,000. The polydispersity of the pectins isolated was studied.Tashkent State Technical University, fax (3712) 29 48 36. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 349–353, May–June, 1998.     

Synthesis of14C-radioactively labeled derivatives of gossypol and the study of their pharmacokinetics

The method of obtaining radioactively labeled¹⁴C-gossypol-acetic acid has been improved: from it have been synthesized¹⁴C-derivatives of gossypol and their complexes with N-polyvinylpyrrolidone. A comparative study has been made of the pharmacokinetics of batriden, megosin, and their water-soluble complexes.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 162 70 71. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 810–818, November–December, 1997.     

A study of the mechanism of the action of extensin-like proteins of the cotton plant in a cell culture

The mechanism of the action of extensin-like proteins (ELPs) isolated from the cotton plant on a cell line of murine melanoma (CML) has been studied from the inclusion of³H-thymidine and by counting live cells. The dose- and phase-dependent inhibitory nature of the action of ELPs on MLC cells has been established.Deceased.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (371) 162 70 71. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 372–375, May–June, 1999.     

V. Mass spectrometry of the alkaloids ofArundo donax

The mass spectra of alkaloids isolated from the epigeal part of the plant Arundo donax L. have been investigated. The main pathways of fragmentation under electron impact have been determined for arundine, ardine, donaxaridine, donaxarine, and donaxinine. An analogy has been found in the fragmentation of the M⁺ ions of donaxarine and donaxinine, the main pathway of their fragmentation consisting in the elimination of the elements of the N-methylpyrrolidone ring.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 217–220, March–April, 1996. Original article submitted September 25, 1995.     

Synthesis of [3H]Abscisic acid and identification of the ABA-bound proteins of the cotton plant

[³H]Abscisic acid has been obtained by heterogeneous catalytic exchange in a tritium-containing solvent. The presence in cottonplant shoots of proteins specifically binding with abscisic acid has been shown.A. S. Sadykov Institute of Bioorganic Chemistry Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 697071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 109–112, January–February, 1994.     

Ir spectroscopy of 2,3-polymethylene-3,4-dihydroquinazolines and their complexes

We have investigated the IR spectra in the region of stretching vibration of N⁺–H and OH groups of the hydrochlorides of 2,3-trimethylene-3,4-dihydroquinazoline (deoxypeganine, DOP), 2,3-tetramethylene-3,4-dihydroquinazoline (tetrazoline), and 2,3-pentamethylene-3,4-dihydroquinazoline (pentazoline) and peganol and peganine and the corresponding complexes with ZnCl₂, CoCl₂, and MnCl₂. The appearance of two additional absorption bands in the 3100–3300 cm^–1 region in the complexes investigated as compared with the corresponding hydrochlorides witnesses the formation of interionic hydrogen bonds between the quinazoline cation and the (MeCl₄)^2– anion.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 6., pp. 854–857, November–December, 1997.     

Biologically active proteins of the wormEisenia foetida

Seven protein-peptide fractions have been isolated from the biomass of the wormEisenia foetida. Their influence on the synthesis of RNA, DNA, and protein in animal and plant cells has been investigated.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 627071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 118–120, January–February, 1994.     

Polysaccharides of saponin-bearing plants XI. An investigation of the polysaccharides ofAcanthophyllum knorringianum

Polysaccharides have been isolated from the epigeal organs and roots ofAcanthophyllum knorringianum and their quantitative levels and qualitative compositions have been established. A neutral polysaccharide of the epigeal organs belongs to the mannoarabinoglucogalactan type, and a neutral polysaccharide from the roots, to the arabinoglucogalactans.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (371) 120 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 169–170, March–April, 1999.     

Low-molecular-mass metabolites of fungi I. Stachybotrin fromStachybotrys alternans

Stachybotrys alternans produces a series of low-molecular-mass compounds. By their column chromatography we have isolated the nitrogen-containing compound stachybotrin. The structure of stachybotrin has been determined on the basis of chemical transformations together with the results of¹H and¹³C NMR spectroscopy, 2M NMR¹H–¹H and¹H–¹³C chemical shift correlation (COSY), correlation of long-range¹H–¹³C interactions (HMBC), measurements of NOEs in a rotating coordinate system (ROESY), and IR and electronimpact mass spectra.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 599–607, July–August, 1997.     

Polysaccharides of saponin-bearing plants. IX. Structures of oligosaccharides from the glucogalactan ofochrusa gypsophiloides

The partial acid hydrolysis of the glucogalactan from A. gypsophiloides has yielded four oligosaccharides, two of which have been obtained for the first time and are branched tetra- and pentasaccharides. Their structures have been established by periodate oxidation, methylation, and reduction and also from the results of¹³C NMR.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 534–536, July–August, 1996. Original article submitted February 20, 1995.     

Panicudine — A new alkaloid fromAconitum paniculatum

A structure proposed for panicudine — a C₂₀-diterpene alkaloid of the hetisine type — has been established on the basis of an analysis of IR, UV, mass and¹H and¹³C NMR spectra.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 838–840, November-December, 1995. Original article submitted March 27, 1995.     

Polysaccharides of saponin-bearing plants. X. An investigation of the carbohydrates ofAcanthophyllum pungens

Polysaccharides have been isolated from the epigeal and hypogeal organs ofAcanthophyllum pungens and their qualitative presence and quantitative compositions have been determined. A neutral polysaccharide from the epigeal organs is of the galactan type, while a neutral polysaccharide from the roots is a glucogalactan.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 693–695, September–October, 1997.     

Synthetic analogs ofPeganum alkaloids VIII. A quantum-mechanical investigation of the reduction of deoxypeganine and its derivatives

The alkaloid deoxypeganine and some of its derivatives have been subjected to reduction with sodium tetrahydroborate and with the complex NaBH₄·BF₃·Et₂O. It has been shown that the structures of the reduction products depend on the presence and positions of substituents in the benzene ring. The quantumchemical calculations performed have confirmed the probable formation of reduced structures of the type ofo-aminobenzylpyrrolidine and the type of a macrocyclic diamine.Deceased.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (371) 120 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 220–225, March–April, 1999.     

Plant polysaccharides. IX. Isolation and anticoagulant activity of the polysaccharides ofLagochilus usunachmaticus

A polysaccharide possessing pronounced direct-action anticoagulant activity has been isolated from the epigeal part ofLagochilus usunachmaticus.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 690–692, September–October, 1997.