载叶黄素白蛋白纳米粒口腔速溶膜的制备及评价

联合应用白蛋白纳米粒和口腔速溶膜技术,以期改善叶黄素的生物利用度并提高患者顺应性。采用反溶剂沉淀法制得叶黄素白蛋白纳米粒、溶剂浇铸法将其载入口腔速溶膜,并对所得速溶膜进行体内外评价。所得膜剂中粒子分散均匀,厚度36μm,耐折度为87次,30 s内迅速崩解。药物以无定形状态存在或高度分散在膜中。与口服溶液相比,SD大鼠舌上给予膜剂后的C_(max)提高了7.02倍,AUC_(0 h~24 h)增大了2.91倍。结果表明,载叶黄素白蛋白纳米粒口腔速溶膜综合了白蛋白纳米粒和口腔速溶膜的双重优点,吸收速度和程度显著提高,应用前景良好。     

多维元素口腔崩解片的研制

以多维元素为原料,加入微晶纤维素和甘露醇等辅料,采用湿法制粒压片,制备了多维元素口腔崩解片.通过筛选处方和优化工艺,确定了产品的制作工艺条件及配方.实验结果表明:原料多维元素用量9.5%、填充剂甘露醇41%,崩解剂微晶纤维素20%、羧甲基淀粉钠17.5%、低取代羟丙基纤维素7.5%,矫味剂阿斯巴甜4%、薄荷香精2%和柠檬酸0.5%,制备的口腔崩解片崩解迅速,口感良好,无沙砾感,服用方便.     

响应面法优化磷酸化改性花生分离蛋白膜制备工艺

目的研究磷酸化改性花生分离蛋白膜的制备工艺方法。方法以磷酸化改性花生分离蛋白为原料,以蛋白浓度、pH值、甘油百分含量(占蛋白)、黄原胶百分含量(占蛋白)、时间、温度、超声波功率、超声波频率为考察因素,以膜厚度、吸水率和透光率为考察指标,在单因素实验基础上,通过响应面实验设计进行工艺优化。结果磷酸化改性花生分离蛋白膜的最优制备工艺条件为蛋白浓度5.4%、p H值9.0、甘油百分含量(占蛋白)23.4%、黄原胶百分含量(占蛋白)4.2%、时间60 min、温度66℃、超声波功率210 W、超声波频率28 kHz;此工艺条件下的膜厚度、吸水率和透光率的响应面模型预测值分别为69μm、44.3%和50.7%,验证实验值分别为70±1μm、45.4%±1.6%和49.8%±1.4%,与模型预测值相差1.45%、2.48%和1.78%,说明模型与实际情况拟合较好,验证了预测模型的正确性。结论响应面法对磷酸化改性花生分离蛋白膜制备工艺条件参数优化是可行的,得到的工艺条件具有实际应用价值。     

半仿生法萃取金银花中绿原酸的研究

为优化金银花中绿原酸提取工艺,以绿原酸含量为评价指标,在单因素试验基础卜进行了正交优化设计得到了半仿生法提取绿原酸的最佳工艺.根据仿生学原理并结合影响中药有效成分提取的各种因素,对提取温度、时间及料液比等3个因素进行研究.结果表明,用金银花为原料,以磷酸二氧钠-柠檬酸缓冲液为萃取剂(pH值分别为2.0、7.5和8.3),料液比(MA(金银花):MB(萃取液)=1:20),在70℃、每次提取1 h、提取3次时,绿原酸含量达到最高,为12.08 μg/mL.     

聚乙二醇修饰木犀草素脂质体的制备及其理化性质

研究聚乙二醇修饰木犀草素脂质体的最佳制备工艺,以提高木犀草素的生物利用度。采用薄膜分散法制备聚乙二醇修饰木犀草素脂质体,以磷脂、胆固醇和磷脂酰乙醇胺-聚乙二醇2000作为载体,包封率为评价指标,采用Box-Behnken设计实验响应面优化法优化工艺参数,分析聚乙二醇修饰木犀草素脂质体的粒径、电位和形态等指标。结果表明聚乙二醇修饰木犀草素脂质体的最佳制备参数为：膜材比为10:1,脂药比为16:1,磷脂酰乙醇胺-聚乙二醇2000的质量分数为3%,超声时间15 min,水化温度40 ℃。Box-Behnken响应面法优化后的聚乙二醇修饰木犀草素脂质体平均包封率为86.3%,平均粒径为134.1 nm,电位为-14.07 mV,且在胃液、小肠液和结肠液中的累积释放率分别为39.08%、43.67%和71.71%。所制备的聚乙二醇修饰木犀草素脂质体包封率高,粒度均匀,缓释效果明显。     

响应面法优化马铃薯薯渣可食性膜封口工艺

采用响应面法对可食性膜的封口工艺进行优化。选取膜厚度,封口时间和封口温度为随机因子。在单因素试验的基础上,根据Box-Benhnken中心组合方法进行三因素三水平的试验设计。以封合强度为响应值,进行响应面分析(RSA),结果表明,可食性膜热封试验的最佳工艺条件为:膜厚度为0.083 mm,封合时间为4.6 s,封合温度140.2℃,在此条件下,可食性膜的封合强度理论值为7.493 N/15 mm,验证实测值为7.471 N/15 mm,与理论值相对误差为0.29%。     

钛酸铋钠基铁电薄膜掺杂工艺研究

论文首次介绍了最新的钙掺杂钛酸铋钠薄膜的金属有机溶液沉积法（MOSD法）-匀胶法甩膜（Spin coating）工艺，提出现行铁电薄膜制备工艺要解决的一些问题。     

玻璃微球法制备水溶性药物脂质体的研究

以牛血清蛋白、胰岛素、谷胱甘肽为水溶性药物模型,采用玻璃微球法制备其脂质体,以包封率为评价指标,研究玻璃微球法制备水溶性药物脂质体的具体工艺。结果表明,在最优工艺下该方法制备牛血清蛋白的包封率为87.9%;制备胰岛素脂质体的包封率为91.7%;制备谷胱甘肽脂质体的包封率为94.1%。制备的脂质体平均粒径为1 393.1 nm,Zeta电位为-51.2 mV。     

壳聚糖-植酸钠膜的制备及性能研究

本试验以壳聚糖和植酸钠为主要原料进行膜的制备。对壳聚糖-植酸钠膜的厚度、不透明度、紫外吸能力、溶胀度、溶解度、透湿性、红外分析和热重分析图进行研究。结果表明:随着植酸钠浓度增加,膜的厚度增加,不透明度也增加;紫外光谱结果表明,植酸钠浓度越高紫外吸收能力越强;随着植酸钠含量增加,膜的溶解度,溶胀度逐渐降低;植酸钠浓度为1.25%时,膜有最低的水蒸气透过率。结论:3%壳聚糖/1.25%植酸钠在室温下的成膜效果最好。     

低糖保健型怀远石榴汁的加工及其感官评价

以怀远石榴为原料,益寿糖为甜味剂,NaCl为单宁去除剂,柠檬酸为护色剂和酸味剂加工低糖保健型的石榴汁饮料,采用模糊综合评判法对其进行感官评价,并对工艺条件进行优化.结果表明,对石榴汁口感影响最大的是甜味剂的添加量,次之是单宁去除剂的添加量.最佳工艺条件为:甜味剂益寿糖的添加量为15％,单宁去除剂NaCl为0.10％,护色/酸味剂柠檬酸为0.20％.根据模糊综合评判法,此条件下所得石榴汁的感官质量最好.     

Na-diclofenac β -cyclodextrin inclusion complex on cotton wound dressing

This paper deals with the production of a wound dressing capable of releasing sodium diclofenac as a drug for curing inflammations. Sodium diclofenac was used as an inclusion compound with β -cyclodextrin and then attached to the cotton wound dressing by a cross-linking agent, namely, dimethyl dihydroxyl ethylene urea (DMDHEU). Here some analyses were performed such as UV spectrophotometry and HNMR spectroscopy to ensure the complex formation of β -cyclodextrin and sodium diclofenac, surface morphology studies by SEM, and dissolution studies for drug release. The results revealed that increasing the amount of complex increases the drug contained in the wound dressing and the cross-linking agent prolongs the drug release time. Also, the existence of ethanol as a solvent for sodium diclofenac increases the drug released during the initial hours of drug release tests.     

Extended delivery of non-steroidal anti-inflammatory drugs through contact lenses loaded with Vitamin E and cationic surfactants

The purpose of this study is to extend drug release from ACUVUE Oasys® and ACUVUE TruEye® silicone hydrogel contact lenses by incorporation of vitamin E in conjunction with a cationic surfactant. In ACUVUE Oasys® and ACUVUE TruEye®, the release of ketorolac tromethamine and flurbiprofen sodium is extended from hours to several days for 11% and 21% vitamin E, (weight of vitamin E / weight of dry lens) but with a considerable reduction in the amount of drug released. Cetalkonium chloride and stearylamine increased the drug loading capacity which was otherwise compromised by the addition of vitamin E in the contact lenses. In the case of diclofenac sodium, a sustained release over 150 h for both contact lenses can be achieved. It was found that the release-time-increase factor due to vitamin E has a linear dependence with the octanol-water partition coefficient of the drug in ACUVUE Oasys®. The results in this study show that contact lenses loaded with vitamin E in conjunction with cationic surfactants achieved sustained release of non-steroidal anti-inflammatory drugs (NSAIDs) within the therapeutic window.     

Drug release from hydrogel containing albumin as crosslinker

Albumin is the major plasma protein and acts as a physiological carrier for various compounds including drugs. To take advantage of the drug-binding ability of albumin for a drug delivery system, we have prepared hydrogels consisting of acrylamide (AAm) and bovine serum albumin (BSA) by introducing three to four vinyl groups into one BSA molecule and subsequently copolymerizing it with AAm. The resultant hydrogel was solubilized by trypsin treatment, since BSA served as a crosslinker in the hydrogel. The BSA-crosslinked hydrogel (BSA-AAm hydrogel) was loaded with salicylic acid or sodium benzoate and their release was investigated. The BSA-AAm hydrogel released much more salicylic acid than sodium benzoate. In addition, the amount of released salicylic acid increased with the BSA content of the hydrogel, despite a decrease in the swelling ratio of the hydrogel. On the other hand, the amount of released sodium benzoate increased with the swelling ratio. When a hydrogel crosslinked with N,N'-methylenebis (acrylamide) was used as a control, both drugs showed release tendencies similar to that of sodium benzoate from the BSA-AAm hydrogel. Furthermore, the salicylic acid release was sustained longer on the BSA-AAm hydrogel than the sodium benzoate release. Taken together, it is thought that albumin in the BSA-AAm hydrogel preferentially adsorbs salicylic acid and contributes to the high drug loading and the sustained release of salicylic acid.     

海藻酸钠薰衣草精油微胶囊的制备及其性能研究

为了提高薰衣草精油的稳定性并且能使精油能控时控释释放。本实验考察了不同浓度的Span 85对乳液质量的影响,同时考察了去稳定剂、海藻酸钠浓度、CaCl₂浓度、固化时间对薰衣草精油微胶囊制备效果的影响,进而得出适宜的薰衣草精油微胶囊制备条件,并对其性能进行了测定,同时研究了不同膜厚度和pH条件下薰衣草精油的释放情况。结果显示,分别加入乙醇、吐温20、乙醇加吐温20后微胶囊的膜厚度从20 μm增加至56 μm。释放结果表明,空白组在10 h内释放量达到了92%,乙醇组为80%,吐温20组为82%,但乙醇加吐温20组释放量仅为70%。此外还对微胶囊的载药量、包封率、粒径、形态等进行了考察,结果显示微胶囊的载药量在38.2%~57.2%之间,包封率在76.4%~92.8%之间,粒径处于45~84 μm之间。综上所述,所制备的微胶囊膜厚度可控、形态圆整均一,薰衣草精油的释放时间长短可通过调节膜的厚度及释放介质的pH来实现。     

甘油对高直链玉米淀粉成膜性能的影响

研究甘油添加量为0～40% 的高直链玉米淀粉糊的流变性能。采用流延法将添加了甘油量为0～40% 的高直链玉米淀粉糊成膜,并对其结晶结构和耐酸性能进行研究。制备以VB1 为功能因子的淀粉薄膜包衣微丸,系统考察不同甘油添加量的高直链玉米淀粉包衣微丸在模拟人体消化道环境中的控缓释性能。结果表明：随着甘油添加量的增加,高直链玉米淀粉糊的黏度下降,膜的结晶性能和耐酸性能下降。通过调节甘油添加量和包衣厚度,使得高直链玉米淀粉能够抵御胃酸的作用而到达小肠,包衣后的微丸具有一定的控缓释功能。     

O-palmitoylcurdlan sulfate (OPCurS)-coated liposomes for oral drug delivery

O-Palmitoylcurdlan sulfate (OPCurS) was applied to the liposomal surface to improve the stability of liposomes. To synthesize OPCurS, curdlan was chemically sulfated and then modified with a palmitoyl derivative. The synthesized OPCurS was characterized by Fourier transform-infrared spectroscopy (FT-IR). OPCurS-coated liposomes prepared by the solvent evaporation method were characterized for size, shape, surface charge, and stability in simulated gastric fluid (SGF) and sodium cholate solution. The sizes of OPCurS-coated liposomes increased with the OPCurS content of liposomes and zeta potential decreased when OPCurS was applied to the liposomal surface. With the increase in the content of OPCurS attached to the liposomal surface, the stability of liposomes in SGF and sodium cholate solution was gradually induced and the stability was most improved at a lipid/OPCurS weight ratio of 1.5. Liposomes not coated with OPCurS released 99.5+/-2.3% of the initial 5-carboxyfluorescein (5-CF) content, whereas OPCurS-coated liposomes released 53.7+/-3.7%. OPCurS on the surface of liposomes suppressed the release of 5-CF. Theses results indicate that OPCurS-coated liposomes can be effectively used as a drug delivery carrier via oral administration.     

壳聚糖亚胺改性棉纤维的结构与性能

 摘 要：采用壳聚糖溶液对经高碘酸钠氧化的棉纤维进行亚胺化功能改性。分析了不同高碘酸钠浓度对棉纤维醛基含量、断裂强力和壳聚糖处理后棉纤维增重率的影响。通过傅立叶红外光谱分析和X射线衍射曲线分峰拟合计算,结果表明,壳聚糖分子借助希夫碱C＝N共价键与氧化棉纤维亚胺交联;氧化棉纤维经壳聚糖处理后结晶度有所降低。壳聚糖改性棉纤维的断裂强力及伸长率下降,初始模量增大。利用制备的壳聚糖改性棉纤维作为药物载体承载与缓释芦荟蒽醌提取物,得到了良好的控制释放药物效果。因此,壳聚糖亚胺改性棉纤维在持续释放药物方面的应用前景广阔。     

VC脂质体水凝胶的制备及其消化特性研究

将脂质体和水凝胶结合,以卡拉胶和乳清分离蛋白通过酶和离子交联形成凝胶,将维生素C(VC)脂质体包埋于其中形成VC脂质体水凝胶。通过表征脂质体水凝胶在不同储存时间的磷(Pi)释放量和脂质氧化程度,以及模拟消化过程中的平均粒径及粒度分布,脂质体和乳清分离蛋白的降解,VC的释放量,研究脂质体水凝胶的储存稳定性和体外消化稳定性。结果表明,VC脂质体水凝胶在30 d储存过程中,外观较为稳定,脂质体释放程度较低,且脂质氧化程度较低;在体外口腔和胃消化中,脂质体水凝胶都保持较为稳定,水凝胶结构不易被破坏,且脂质体的包封效果好。进入模拟小肠环境后,脂质体开始大量从水凝胶游离出来,结构被破坏,释放包裹的VC。本试验制备了能较长时间储存,并在小肠内定点释放的脂质体水凝胶运载体系,可用于食品领域包埋多种营养素。     

Antimicrobial performance of potassium sorbate supported in tapioca starch edible films

The release and antimicrobial activity of potassium sorbate (KS) supported in tapioca starch–glycerol edible films prepared by different gelatinization/drying techniques against Zygosaccharomyces bailii was studied. Antimicrobial release in liquid media of different pHs (3.0–6.0) could be approximated to a pseudo first-order kinetic model and was almost accomplished after 30 min. Filmmaking method involving slow gelatinization and drying rate resulted in the highest fraction of KS released at equilibrium. Rate constant was higher when pH of the receiving media was 4.5 and fast gelatinization/fast drying had been used. The effectiveness of the preservative released for controlling the microbial growth depended on the pH of the receiving media, being higher at pH 3.0. No effect of filmmaking method was observed. In relation to film effectiveness as a barrier to contamination, it was observed that the preservative was available to prevent an external Z. bailii contamination and also to control yeast growth in an acidified (pH 4.5) high water activity (a _w = 0.980) semisolid product.     

玉米醇溶蛋白膜的制备及其保鲜效果评价

该实验制备了负载大蒜素-丁香酚的玉米醇溶蛋白膜,并评价其对圣女果的保鲜效果。研究表明大蒜素-丁香酚混合精油的加入量为1.50%时（体积分数）,所制得的玉米醇溶蛋白膜在外观、厚度、透明度等方面表现良好。用此配方对圣女果进行覆膜处理,负载大蒜素和丁香酚的蛋白膜与空白膜和对照相比,在贮藏期间圣女果的失重率、腐烂软化率、褐变度均小于空白对照组,在第8天各项指标分别较对照降低22.92%、50.00%、19.11%;圣女果的维生素C含量在第8天较对照提高1.69%,在第10天可溶性糖含量较对照提高9.58%。实验结果表明,负载大蒜素-丁香酚的玉米醇溶蛋白膜在减少圣女果水分流失、抑制腐烂和抗氧化等方面的性能均有了明显的提高,可以进一步开发成蔬果保鲜剂进行应用。