Modelling of coliform removal in 186 facultative and maturation ponds around the world

The paper presents a very extensive evaluation of the coliform decay in facultative and maturation ponds, based on data from 186 different ponds in the world. The ponds encompass a very wide diversity in terms of physical and operating conditions, covering most situations encountered in practice. The median values for the coliform removal efficiencies were 1.8 log units (98% removal) for primary facultative ponds, 1.0 log units for secondary facultative ponds (90% removal) and 1.2 log units (94% removal) for each maturation pond in the series. Two equations to be used for design purposes were derived for estimating the die-off coefficient K(b) (dispersed flow, 20 degrees C) in facultative and maturation ponds. The first equation led to a slightly better fitting with the observed logarithm of the effluent coliform concentrations (R2 = 0.874), and related K(b) with the pond detention time t and depth H (K(b) = 0.682 H(-1.286) t(-0.103)). The other equation also led to a satisfactory fitting (R2 = 0.845), but was slightly simpler, depending only on the pond depth (K(b) = 0.549 H(-1.456)).     

Removal of organic matter in stormwater ponds: a plug-flow model generalisation from waste stabilisation ponds to shallow rivers

ABSTRACT

This study investigates the effect of flow conditions on the organic matter concentration, removal efficiency, and reaction kinetics in a stormwater pond in Fortaleza, Brazil. As a result of unauthorized sewage discharges, BOD and COD concentrations were similar to those of combined sewer systems. The concentrations remained roughly the same during the rainy season, which was attributed to sewage network overflow. Removal efficiencies ranged from 70–90%, similar to primary facultative ponds. Fitting different hydraulic models to the field data, it was possible to obtain BOD and COD removal rates that could be described as functions of the Reynolds number. The best fit was achieved considering the plug-flow assumption, and a general BOD model including data reported in the literature for waste stabilisation ponds, stormwater ponds and shallow rivers was derived. Lastly, simulations with this general model were performed to assess the impact of remediation measures on the studied pond.     

Optimisation of hydraulic performance to maximise faecal coliform removal in maturation ponds

The present study was conducted with the aim of improving faecal coliform (FC) and faecal streptococcus (FS) removal efficiencies in tertiary maturation stages of a sewage treatment plant in Southern England, where climatic conditions are sub-optimal. The research used intensive field assessments (bacteriological, general quality and hydraulic) to identify the parameters that affect the bacteriological quality of the effluent from three parallel maturation ponds (North, Central and South) of similar geometry and dimensions. An engineering intervention was carried out to convert the South pond to three channels to increase the L/W ratio from 9:1 to 79:1. Hydraulic tracer studies in the South pond with Rhodamine WT showed that the dispersion number 'd' was reduced from 0.37 (dispersed flow) to 0.074 by this intervention under similar flow conditions (4.5l/s). Hydraulic retention time was thus increased by 5h, delay in jet flow short-circuiting was increased from 2.5 to 17.5h thus increasing the exposure times for all elements. As a result of the intervention FC removal increased substantially. Maximum channel-lagoon efficiency of 99.84% was obtained at 4.5l/s and 19 degrees C, when exposure to sunlight was 17 h in summer. It is concluded that the channel configuration produces a higher hydraulic efficiency than conventional maturation ponds. It is therefore recommended as a viable engineering solution which permits a low-cost upgrading of plant performance, requiring no additional land, and with minimal maintenance costs.     

Indicator bacteria and limnological parameters in fish ponds

The populations of indicator bacteria (mesophilic, coliform and fecal streptococci) together with relevant limnological parameters (temperature, oxygen, BOD and chlorophyll-) were recorded during a 6 year study of three eutrophic ponds. Ecosystems were manipulated with fish (Cyprinus carpio) in 2-year management cycles: in spring of the first year, the pond was stocked with young fish, and in the fall of the second year it was drained and mature fish were harvested. Fish management had a direct influence on bacterial numbers and on the basic parameters of the systems. A statistical analysis using t-tests found significant differences in oxygen concentration, chlorophyll- and BOD during both years of fish management. A water temperature model was calculated on an extensive homogenous data set (197 measurements), by fitting a regression curve. During each season, populations of indicator bacteria increased with increasing water temperature, and maximal numbers of bacteria were recorded during the summer months. Correlation analysis (fitted Spearman correlation coefficient) confirmed that temperature had a positive significant effect on population dynamics. The results demonstrate that fish stocking can affect bacterial population, in the sense that during high fish biomass (second years) the numbers of indicator bacteria as well as BOD and phytoplankton were higher. If pond water quality must be optimised for the purposes of drinking or recreation, the planned fish yield may have to be reduced.     

Efficiency of natural systems for removal of bacteria and pathogenic parasites from wastewater

A combined constructed wetland formed by a facultative pond (FP), a surface flow wetland (SF) and a subsurface flow wetland (SSF) was studied from December 2004 until September 2005 in north-western Spain in order to evaluate their efficiency in the removal of pathogenic and indicator microorganisms and to determine their relationships. Microbial removal ranged from 78% for coliphages to over 99% for helminth eggs, depending on the treatment system. The highest removal of indicator bacteria (total coliforms, E. coli, faecal streptococci and Clostridium perfringens) occurred in the stabilization pond, reaching 84%, 96%, 89% and 78%, respectively. However, the greatest removal of protozoan pathogens (Cryptosporidium and Giardia) and coliphages was found in the SSF wetland, 98%, 97% and 94%, respectively. In contrast, the SF wetland was most efficient in the removal of pathogenic parasites when considering superficial removal rates. Seasonal differences in organism removal were not statistically significant during the study period. First-order removal rate constants ranged from 0.0027 to 0.71 m/d depending on the microorganism and type of wetland. Significant correlations were found between pathogenic parasites and faecal indicators in the influent of the treatment system but not in the other sampling points suggesting that such relations varied along the system due to the different survival rates of the microorganisms.     

Mass spectrometric and toxicological assays of Athabasca oil sands naphthenic acids

This work concerns the analysis of model naphthenic acids and authentic naphthenic acids from the tailings ponds of the Athabasca tar sands. A first objective was to compare atmospheric pressure chemical ionization mass spectrometry (APCI-MS) with the previously studied electrospray mass spectrometry (ESI-MS) in this analysis. APCI-MS had a wider range of quantitation than ESI-MS, but its detection limit was poorer and model compounds showed greater variation in calibration sensitivity. A second objective was fractionation of naphthenic acids from tailings pond water and analysis by the Microtox toxicity assay. Fractionation on the basis of solubility gave fractions that did not differ significantly either in their congener distribution by ESI-MS or in their response to the Microtox assay. When partial separation was achieved by anion exchange chromatography, fractions with a higher proportion of multi-ring structures exhibited lower toxic potency. This finding is consistent with field observations that indicate that the toxic potency of tailings ponds water declines as the samples age-multi-ring structures are more highly branched and therefore more resistant to microbial degradation.     

Comparison of ammonia volatilisation rates in algae and duckweed-based waste stabilisation ponds treating domestic wastewater

Quantification of ammonia volatilisation from wastewater stabilisation ponds is important in order to understand its significance for overall nitrogen removal in these widely applied low-cost treatment systems. Ammonia volatilisation rates were measured in pilot plant facilities consisting of one line of four algae-based ponds in series and a parallel line of four ponds with a floating mat of duckweed (Lemna gibba). Ammonia volatilisation was assessed during a period of one and a half years. The method applied is accurate, convenient and is proposed for analysis of a wide range of gasses emitted from stabilisation ponds and possibly other aquatic systems. The ammonia volatilisation rates in algae-based ponds (ABPs) were higher than in duckweed-based ponds (DBPs). This can be explained by the lower values of NH(3) in DBPs due to shading and lower pH values, since the volatilisation rate highly correlated with free ammonia concentration (NH(3)) in pond water. The duckweed cover appeared not to provide a physical barrier for volatilisation of unionised ammonia, because whenever NH(3) concentrations were equal in ABP and DBP also the volatilisation rates were equal. Volatilisation was in the range of 7.2-37.4 mg-Nm(-2)d(-1) and 6.4 -31.5 mg-Nm(-2)d(-1) in the ABPs and DBPs, respectively. Average influent and effluent ammonium nitrogen measurements showed that the ammonia volatilisation during the study period in any system did not exceed 1.5% of total ammonium nitrogen removal. Therefore this study confirmed results from simultaneous experimental work in our laboratory indicating that nitrification/denitrification, rather than ammonia volatilisation, is the most important mechanism for N removal in ABPs and DBPs.     

Impact of oil and oil-dispersant mixtures on flora and water chemistry parameters in freshwater ponds

Oil and oil-dispersant mixtures at nominal concentrations of 100 and 20 ppm, respectively, were added to a series of ponds constructed for the study. In the pond treated with oil, there were no discernible short or long term effects on the phytoplankton. Both oil-dispersant treated ponds exhibited fluctuations in the dominant class of algae while the concentration of oil was greater than in the water column. Once the oil concentrations were below this value, there was no apparent effect. Periphytic material on the sides and bottoms of the oiled and control ponds were similar in mass and composed of a large number of species. Periphyton biomass was at least three times greater in both oil-dispersant-treated ponds with one genus dominating the growth. These conditions persisted in one of the oil-dispersant-treated ponds one year after treatment but those in the other pond had decreased to levels in the control pond at this time. Dissolved oxygen (DO) values decreased to about 4.6 ppm in both oil-dispersant-treated ponds shortly after treatment, but remained at approximately the saturation level in the other ponds for 6 weeks after treatment. Then these lower values gradually increased until they were slightly greater than in the controls. During the late winter months, both oil-dispersant ponds had anoxic zones above the sediment, the extent depending on the contours of the bottom. The DO values in the other ponds were at the saturation level. In the early spring, the nitrate ion-concentrations in control and oil-treated ponds were twice those measured for the oil-dispersant ponds. No discernible differences dependent on treatment were observed for nutrients or other ions monitored on a regular basis.     

Impact of population and latrines on fecal contamination of ponds in rural Bangladesh

A majority of households in Bangladesh rely on pond water for hygiene. Exposure to pond water fecal contamination could therefore still contribute to diarrheal disease despite the installation of numerous tubewells for drinking. The objectives of this study are to determine the predominant sources (human or livestock) of fecal pollution in ponds and examine the association between local population, latrine density, latrine quality and concentrations of fecal bacteria and pathogens in pond water. Forty-three ponds were analyzed for E. coli using culture-based methods and E. coli, Bacteroidales and adenovirus using quantitative PCR. Population and sanitation spatial data were collected and measured against pond fecal contamination. Humans were the dominant source of fecal contamination in 79% of the ponds according to Bacteroidales measurements. Ponds directly receiving latrine effluent had the highest concentrations of fecal indicator bacteria (up to 10⁶ Most Probable Number (MPN) of culturable E. coli per 100 mL). Concentrations of fecal indicator bacteria correlated with population surveyed within a distance of 30-70 m (p < 0.05) and total latrines surveyed within 50-70 m (p < 0.05). Unsanitary latrines (visible effluent or open pits) within the pond drainage basin were also significantly correlated to fecal indicator concentrations (p < 0.05). Water in the vast majority of the surveyed ponds contained unsafe levels of fecal contamination attributable primarily to unsanitary latrines, and to lesser extent, to sanitary latrines and cattle. Since the majority of fecal pollution is derived from human waste, continued use of pond water could help explain the persistence of diarrheal disease in rural South Asia.     

Fecal indicator bacteria and Salmonella in ponds managed as bird habitat, San Francisco Bay, California, USA

Throughout the world, coastal resource managers are encouraging the restoration of previously modified coastal habitats back into wetlands and managed ponds for their ecosystem value. Because many coastal wetlands are adjacent to urban centers and waters used for human recreation, it is important to understand how wildlife can affect water quality. We measured fecal indicator bacteria (FIB) concentrations, presence/absence of Salmonella, bird abundance, and physico-chemical parameters in two coastal, managed ponds and adjacent sloughs for 4 weeks during the summer and winter in 2006. We characterized the microbial water quality in these waters relative to state water-quality standards and examined the relationship between FIB, bird abundance, and physico-chemical parameters. A box model approach was utilized to determine the net source or sink of FIB in the ponds during the study periods. FIB concentrations often exceeded state standards, particularly in the summer, and microbial water quality in the sloughs was generally lower than in ponds during both seasons. Specifically, the inflow of water from the sloughs to the ponds during the summer, more so than waterfowl use, appeared to increase the FIB concentrations in the ponds. The box model results suggested that the ponds served as net wetland sources and sinks for FIB, and high bird abundances in the winter likely contributed to net winter source terms for two of the three FIB in both ponds. Eight serovars of the human pathogen Salmonella were isolated from slough and pond waters, although the source of the pathogen to these wetlands was not identified. Thus, it appeared that factors other than bird abundance were most important in modulating FIB concentrations in these ponds.     

Survival dynamics of fecal bacteria in ponds in agricultural watersheds of the Piedmont and Coastal Plain of Georgia

Animal agriculture in watersheds produces manure bacteria that may contaminate surface waters and put public health at risk. We measured fecal indicator bacteria (commensal Escherichia coli and fecal enterococci) and manure pathogens (Salmonella and E. coli 0157:H7), and physical-chemical parameters in pond inflow, within pond, pond outflow, and pond sediments in three ponds in agricultural watersheds. Bishop Pond with perennial inflow and outflow is located in the Piedmont, and Ponds A and C with ephemeral inflow and outflow in the Coastal Plain of Georgia. Bromide and chloride tracer experiments at Bishop Pond reflected a residence time much greater than that estimated by two models, and indicated that complete mixing within Bishop Pond was never obtained. The long residence time meant that fecal bacteria were exposed to solar UV-radiation and microbial predation. At Bishop Pond outflow concentrations of fecal indicator bacteria were significantly less than inflow concentrations; such was not observed at Ponds A and C. Both Salmonella and E. coli 0157:H7 were measured when concomitant concentrations of commensal E. coli were below the criterion for surface water impairment indicating problems with the effectiveness of indicator organisms. Bishop Pond improved down stream water quality; whereas, Ponds A and C with ephemeral inflow and outflow and possibly greater nutrient concentrations within the two ponds appeared to be less effective in improving down stream water quality.     

Comparison of model approaches for predicting coliform removal in waste stabilization ponds

This paper compares the two most frequently used approaches for the prediction of faecal coliforms in the effluent (FC_e) of waste stabilization ponds (WSPs): the continuous stirred reactor (CSTR) model and the dispersed flow (DF) model. On the basis of theoretical and practical analysis, it is concluded that, under most conditions usually found in practice, the DF model can be expected to predict unrealistically low FC_e. This trend is more pronounced the more the flow conditions in the pond(s) approach plug flow, and the higher the average retention time per pond. Model differences in FC_e prediction typically deviate from one another by up to 1–2 log units. This can lead to the design of substantially different pond sizes, with footprint differences between the two models of 30–50% not being exceptional. Consequently, on the basis of usually applied model parameters, for the time being it appears safer to use the CSTR model for FC_e prediction in WSP systems.     

Bod removal rates of waste stabilization ponds as a function of loading, retention time, temperature and hydraulic flow pattern

This study evaluates the influence of loading, detention time and temperature on the first order BOD removal coefficient K₁. Numerical values of K₁ are derived from semi-continuous-flow laboratory units of sewage ponds and introduced into the design formula for a multi-stage continuous flow reactor. There was a good correspondence (r = 0.92) between the computed BOD removal rates and the empirical results obtained from three large-scale multi-stage pond systems.     

Elimination of Salmonella in stabilization ponds

While the reduction of putrescible organic matter is important in sewage treatment, the microbiological quality of the effluents is of great public health significance, since these are usually discharged into streams or onto land. The performance of two types of stabilization ponds located at Nagpur, India, was studied with respect to the reduction of Salmonella, Coliforms, E. coli and faecal streptococci. The two ponds were operated at different BOD loading and detention time. Furthermore while one had two cells with submerged interconnection the other had three cells interconnected with a surface overflow arrangement. Comparing the results of these two ponds, treating domestic sewage, it is found that Salmonella does not get eliminated in the two celled pond while they are absent in 1 litre aliquots of effluent samples from the three celled pond. The indicator organisms are reduced to a great extent in the latter pond as compared to the former. From this it appears that the number of cells and the interconnecting arrangements are very important besides the loading, detention period and other design features.     

Nitrogen mass balance across pilot-scale algae and duckweed-based wastewater stabilisation ponds

Nitrogen removal processes and nitrogen mass balances in algae-based ponds (ABPs) and duckweed (Lemna gibba)-based ponds (DBPs) were assessed during periods of 4 months, each under different operational conditions. During periods 1 and 2, the effect of cold and warm temperature was studied. During periods 2 and 3, the effect of low- and high-system organic loading (OL) was studied in warm seasons operation. The pilot-scale systems consisted of four similar ponds in series fed with domestic sewage with hydraulic retention time of 7 days in each pond. Overall nitrogen removal was higher during warm temperature in both ABPs and DBPs, but similar during periods 2 and 3. Nitrogen removal in DBPs was lower than in ABPs by 20%, 12% and 8% during cold temperature, warm temperature and high-OL periods, respectively. Depending on temperature and OL rate, ABPs showed higher nitrogen removal via sedimentation (46-245% higher) compared to DBPs. Also, ABPs also showed higher nitrogen removal via denitrification (7-37% higher) compared to DBPs. Ammonia volatilisation in both systems did not exceed 1.1% of influent total nitrogen during the entire experimental period. N uptake by duckweed corresponds to 30% of the influent nitrogen during warm/low OL period and decreased to 10% and 19% during the cold and warm/high OL period, respectively. Predictive models for nitrogen removal presented a good reflection of nitrogen fluxes on overall nitrogen balance under the prevailing experimental conditions.     

An analysis of cascade‐aerated facultative waste stabilisation ponds in the United Kingdom

This paper examines the performance of cascade‐aerated facultative ponds in a waste stabilisation pond (WSP) installation in the United Kingdom. The pond system is privately owned and treats wastewater from a conference centre with a population equivalent between 20 and 40. A snapshot analysis investigates the removal efficiency of BOD, COD, SS, FC and nutrients. Further, the oxygen supply through ‘flow form’ cascades is examined, and the DO concentrations, surface loads and retention times are discussed. The results show a very high removal of BOD, COD and SS; and nutrient removal is satisfactory. An unusual finding is that the FC removal efficiency is almost two log units better than for a common maturation pond. This is possibly attributable to the high cascade flow, where wastewater is exposed to the damaging effects of sunlight (photo‐oxidation).     

Sunlight, season, snowmelt, storm, and source affect E. coli populations in an artificially ponded stream

Reducing fecal indicator bacteria, such as Escherichia coli (E. coli), in streams is important for many downstream areas. E. coli concentrations within streams may be reduced by intervening ponds or wetlands through a number of physical and biological means. A section of Dunes Creek, a small coastal stream of southern Lake Michigan, was impounded and studied for 30 months from pre-through post-construction of the experimental pond. E. coli reduction became more predictable and effective with pond age. E. coli followed the hydrograph and increased several-fold during both rainfall and snowmelt events. Seasonally, the pond was more effective at reducing E. coli during summer than winter. Late summer, non-solar reduction or inactivation of E. coli in the pond was estimated at 72% and solar inactivation at 26%. E. coli DNA fingerprinting demonstrated that the winter population was genetically more homogeneous than the summer population. Detection of FRNA coliphages suggests that there was fecal contamination during heavy rain events. An understanding of how environmental factors interact with E. coli populations is important for assessing anticipated contaminant loading and the reduction of indicator bacteria in downstream reaches.     

THE PERFORMANCE OF PILOT-SCALE PRIMARY-FACULTATIVE WASTE-STABILISATION PONDS IN THE UK

Three pilot-scale primary-facultative waste stabilisation ponds were constructed at Esholt sewage-treatment works, Bradford. Each pond received screened sewage at different BOD loading rates over a two-year period. Concentrations of BOD, SS, amm. N and chlorophyll-a were measured weekly in summer and bi-weekly in winter, and sludge accumulation was measured after 3, 9, 15 and 20 months. BOD and SS removals were consistently high, although they were lower in summer than in winter due to high levels of algae. The removal of amm, N was generally much better in summer than in winter - the removal mechanism being most likely related to algal activities. Sludge accumulation rates were very low.     

Performance of constructed evaporation ponds for disposal of smelter waste water: a case study at Portland Aluminum, Victoria, Australia

The construction of evaporative ponds and wetlands for the disposal of waste water high in ionic concentrations is a waste disposal strategy currently considered by many industries. However, the design, construction and management of these ponds and wetlands are not straightforward as complex chemical interactions result in both spatial and temporal changes in water quality. The effects of evaporation and drainage on the water quality in two constructed ponds, an adjacent man-made wetland and local groundwater at Portland Aluminium were investigated. The minimum volume of water entering the ponds during the study period was 0.96 +/- 0.16 ML per month. The predicted theoretical evaporative capacity of the two ponds was calculated to be 0.30 +/- 0.07 ML per month. More water enters the ponds than it is theoretically possible to evaporate under the ambient weather conditions at Portland, yet the ponds do not overflow, suggesting percolation through the pond lining. No spatial differences in solute concentrations (fluoride, sulphate, bicarbonate, carbonate, sodium, potassium, calcium, and magnesium ions) were found within the waters of either pond, although temporal differences were apparent. The results support the conclusion that the ponds are not impermeable, and that much of the waste water entering the ponds is being lost through seepage. The impacts on local groundwater chemistry of this seepage are addressed. Significant correlations exist between solute presence within and between the ponds. wetland and groundwater. Fluoride and sulphate concentrations were significantly higher in pond waters throughout the duration of the experiment. Pond sediments revealed a high degree of spatial and temporal heterogeneity in the concentration of all monitored ions resulting from the chemical heterogeneity of the material making up the pond linings. Adsorption isotherms for fluoride indicate that the adsorption capacity of the pond linings remains high for this ion. Implications for the management of waste water by this strategy are discussed.     

Enteric virus survival in algal-bacterial wastewater treatment systems—I : Laboratory studies

The survival of a representative enteric virus, poliovirus type 1, in laboratory models of algal-bacterial sewage treatment systems and the interactions of poliovirus with stabilization pond water was studied. In laboratory reactors a fraction of the total poliovirus present rapidly adsorbed to stabilization pond water solids by a reversible process, conforming to a Freundlich adsorption isotherm. In laboratory cultures the growth of Scenedesmus quadricauda and Bacillus megaterium in sterile sewage had no detrimental effect on poliovirus survival, whereas the growth of heterogeneous populations of stabilization pond bacteria in the same medium resulted in substantial virus inactivation. Appreciable poliovirus inactivation occurred in laboratory cultures of mixed algal-bacterial populations obtained from stabilization ponds. Because the degree of antiviral activity in these cultures was greater than that in cultures of stabilization pond bacteria alone, additional microbial factors must contribute to this virus inactivating phenomenon. The results of this study suggest that in algal-bacterial treatment systems both virus adsorption to solids and virus inactivation due to microbial activity play a role in reducing the enteric virus concentration in wastewater.