A mechanistic model of runoff-associated fecal coliform fate and transport through a coastal lagoon

Fecal coliform (FC) contamination in coastal waters is an ongoing public health problem worldwide. Coastal wetlands and lagoons are typically expected to protect coastal waters by attenuating watershed pollutants including FC bacteria. However, new evidence suggests that coastal lagoons or marshes can also be a source of high indicator organism concentrations in coastal waters. We asked for a Mediterranean-type climate, what is the fate of runoff-associated FC through a coastal lagoon? To address this question, we developed a mass balance-based, mechanistic model of FC concentration through a coastal lagoon and simulated, for summer and winter conditions, FC within the lagoon water column, lagoon sediments, and in the ocean water just downstream of the lagoon mouth. Our model accounts for advective flow and dispersion, decay and sedimentation and resuspension of FC-laden sediments during high flow, erosional conditions. Under low flow conditions that occur in the summer, net FC decay and FC storage in lagoon sediments are predicted. Under high flow conditions that occur in the winter, FC-laden sediments are predicted to erode, resuspend and flow out of the lagoon where they elevate FC concentrations in the coastal ocean. For both seasonal conditions, the predicted water column FC concentrations were within an order of magnitude of field measurements for a reference site in southern California. Our results suggest that there are seasonally varying roles for coastal lagoons in mediating FC contamination to coastal waters.     

Pilot-scale evaluation of design criteria for anaerobic photosynthetic lagoons treating fellmongery (unhairing) wastewater

Fellmongery wastewaters, from the unhairing of sheep pelts, were treated in a pilot-scale (4.7 m³) anaerobic photosynthetic lagoon in which the dominant microorganisms were the purple sulphur bacteria of the family, Chromatiaceae.Removals of 86% COD and 85% sulphide were obtained at a retention time of 90 days. Values of the organic and sulphide removal rate coefficients and the residual COD and sulphide concentrations are presented. The effluent quality compared favourably with predictions based upon models developed in earlier work with laboratory-scale (0.09 m³) lagoons that treated a synthetic fellmongery wastewater under controlled conditions.It was concluded that lagoons in which the Chromatiaceae are the dominant population can provide a high degree of treatment for fellmongery wastewater and that laboratory-scale models can adequately predict effluent quality from such lagoons.     

The modified SWAT model for predicting fecal coliforms in the Wachusett Reservoir Watershed, USA

This study assessed fecal coliform contamination in the Wachusett Reservoir Watershed in Massachusetts, USA using Soil and Water Assessment Tool (SWAT) because bacteria are one of the major water quality parameters of concern. The bacteria subroutine in SWAT, considering in-stream bacteria die-off only, was modified in this study to include solar radiation-associated die-off and the contribution of wildlife. The result of sensitivity analysis demonstrates that solar radiation is one of the most significant fate factors of fecal coliform. A water temperature-associated function to represent the contribution of beaver activity in the watershed to fecal contamination improved prediction accuracy. The modified SWAT model provides an improved estimate of bacteria from the watershed. Our approach will be useful for simulating bacterial concentrations to provide predictive and reliable information of fecal contamination thus facilitating the implementation of effective watershed management.     

Environmental heterogeneity patterns and assessment of trophic levels in two Mediterranean lagoons: Orbetello and Varano, Italy

The management of coastal lagoons is of particular interest due to their high economical importance. In spite of their great productivity, coastal lagoons are often impacted by human pressure which produces water eutrophication. The aim of this paper is to assess the trophic state of the two Mediterranean lagoons taking into account chemical-physical parameters, nutrient concentrations and biological parameters. Two Italian lagoons, Orbetello and Varano (respectively located in Tyrrhenian and Adriatic coast, Italy) were studied between May 2003 and April 2005. Both these systems receive treated urban outflows, agricultural effluents and rivers freshwater inputs. Field collected data showed that studied lagoons were characterized by different human and natural pressures. Orbetello showed the highest water eutrophication, highlighted by the trophic index values, while Varano showed lower eutrophication levels except for the summertime. The values of physical, chemical and biological parameters measured in Orbetello and Varano lagoons indicate that a wide spatial and seasonal gradient of the water characteristics was established during the study period, but in particular in winter. This gradient, typical of estuarine systems, was essentially due to the mixing of freshwater, seawater and anthropogenic inputs. Orbetello lagoon seemed much more affected by the urban impact and the fish-farming activities than Varano lagoon, but the latter showed a greater agriculture activities impact as showed by the remote sensing images.     

Modeling the dry-weather tidal cycling of fecal indicator bacteria in surface waters of an intertidal wetland

Recreational water quality at beaches in California and elsewhere is often poor near the outlets of rivers, estuaries, and lagoons. This condition has prompted interest in the role of wetlands in modulating surface water concentrations of fecal indicator bacteria (FIB), the basis of water quality standards internationally. A model was developed and applied to predict the dry-weather tidal cycling of FIB in Talbert Marsh, an estuarine, intertidal wetland in Huntington Beach, California, in response to loads from urban runoff, bird feces, and resuspended sediments. The model predicts the advection, dispersion and die-off of total coliform, Escherichia coli, and enterococci using a depth-integrated formulation. We find that urban runoff and resuspension of contaminated wetland sediments are responsible for surface water concentrations of FIB in the wetland. Model predictions show that urban runoff controls surface water concentrations at inland sites and sediment resuspension controls surface water concentrations near the mouth. Direct wash-off of bird feces into the surface water is not a significant contributor, although bird feces can contribute to the sediment bacteria load. The key parameters needed to accurately predict FIB concentrations, using a validated hydrodynamic model, are: the load due to urban runoff, sediment erodibility parameters, and sediment concentrations and surface water die-off rates of enteric bacteria. In the present study, literature values for sediment erodibility and water column die-off rates are used and average concentrations of FIB are predicted within 1/2 log unit of measurements. Total coliform are predicted more accurately than E. coli or enterococci, both in terms of magnitude and tidal variability. Since wetland-dependent animals are natural sources of FIB, and FIB survive for long periods of time and may multiply in wetland sediments, these results highlight limitations of FIB as indicators of human fecal pollution in and near wetlands.     

Factors responsible for rapid dissipation of acidic herbicides in the coastal lagoons of the Camargue (Rhône River Delta, France)

This study was aimed at investigating which processes cause acidic herbicides (e.g., bentazone, MCPA and dichlorprop) to rapidly disappear in the lagoons of the Rhône delta, which are peculiar brackish and shallow aquatic environments. The use of the model MASAS (Modeling of Anthropogenic Substances in Aquatic Systems) revealed that sorption, sedimentation, volatilization, flushing and abiotic hydrolysis had a minor role in the attenuation of the investigated herbicides. Laboratory scale biodegradation and photodegradation studies were conducted to better assess the significance of these two processes in the natural attenuation of herbicides in brackish (lagoons) waters with respect to fresh waters (canals draining paddy fields). Herbicide biodegradation rates were significantly lower in lagoon water than in canal water. Consequently, photodegradation was the main dissipation route of all investigated herbicides. The contribution of indirect photolysis was relevant for MCPA and dichlorprop while direct photolysis dominated for bentazone removal. There is a need to further investigate the identity of phototransformation products of herbicides in lagoons.     

Cold region wastewater lagoon sludge accumulation

The rate and extent of solids accumulation in the Palmer and Galena, Alaska, aerated wastewater lagoons and the Logan and Corinne, Utah, facultative wastewater lagoons were investigated. In situ analyses of the sludge layers in each of the four lagoons were performed in the early spring, following the winter ice melt. Sludge core samples and depth measurements were taken over a uniform grid pattern. The rate and extent of solids accumulation were found to vary depending upon lagoon type and specific operational and environmental conditions.     

Impact of inland pollution sources on the bacteriological water quality of the Southern Ganghwado Bay Area,South Korea

The bacteriological pollution of water is a serious global environmental issue. The pollution of water by human and animal wastes can be a source of hazardous pathogens, which may pose serious health risks. In this study, we evaluated the microbiological water quality in the southern Ganghwado Bay area, South Korea. A total of 30 samples (14 discharge water samples from the drainage area and 16 sea water samples from the adjacent area) were analyzed for the concentrations of total coliforms (TC) and fecal coliforms (FC) in November 2013. The highest concentration of FC (28,000 MPN/100 mL) was found at site L1 (inland). The highest mean concentrations of coliforms were found at sites W9 (sea water) and W11 (sea water), with TC and FC concentrations of 33 MPN/100 mL. In conclusion, site L1 located near W9 and W11 is clearly identified as the main source of pollution at Dongmak Beach.     

Computational modelling of large aerated lagoon hydraulics

A good understanding of the hydraulic performance of aerated lagoons is required for their design and operation. A comprehensive numerical procedure has been developed for the three-dimensional computational modelling of the flow in large lagoons including high-speed floating mechanical surface aerators. This paper describes the procedure that consists of separate aerator modelling, then applying the obtained results as boundary data for a full lagoon model. A model application to an industrial aerated lagoon serves as an example of flow analysis. Post processing of the results by calculating the local average residence time (age of fluid) provides a powerful and intuitive technique to visualize and analyse the lagoon performance. The model has been verified by comparing the local average residence time predictions with measurements from a dye study. It is shown that the numerical modelling proposed is feasible and constitutes an effective new tool in improving the performance and design of industrial lagoons.     

Tracer study and profiling of a tertiary lagoon in the United Kingdom: II

Monitoring data over a year showed that the performance of a tertiary lagoon system in the United Kingdom was very variable. Distinct seasonal patterns were seen for all the determinands. Tracer studies and profile sampling of one of the lagoons were carried out in order to investigate the factors affecting performance. The tracer studies, using sodium fluoride, indicated that the hydraulic regime was dispersed plug flow. The mean retention time was found to be 26 h, but the peak in tracer concentration occurred after 12 h, showing significant short-circuiting. During the profile sampling, the pH, temperature, DO and conductivity were measured down through the lagoon at 16 positions and four depths. E. coli, enterococci and total coliform numbers were measured in samples taken from the surface and the base of the lagoon. The results showed temperature decreased down through the lagoon, but the other parameters increased with depth, indicating that the short-circuiting was caused by the cooler influent sinking to the base of the lagoon.     

Wind-aerated lagoons for sustainable treatment of wastewaters from small communities

Biological treatment processes that utilise wind‐aerated lagoons have proved popular for small communities because of their negligible sludge production. Scottish Water has recently constructed one such system, known as the Aero‐Fac (North Dakota, USA), and this paper reports on an intensive 12‐month study designed to both monitor the lagoon performance and establish the key design parameters. Over the monitoring period the final effluent complied with both Scottish Environmental Protection Agency discharge consents and Scottish Water performance guarantees for biochemical oxygen demand and total suspended solids, which averaged 9 and 28 mg/L, respectively. The lagoons showed an accelerated growth of algae during the summer months, but this did not adversely affect the final effluent suspended solids. The lagoons also achieved a faecal coliform removal of around 3.4 log and an average effluent ammonia of 7.6 mg/L. However, the ammonia removal was seasonal with a better performance in the summer months, which probably reflects take‐up by the growing algal population over this period. No sludge was wasted from the lagoon over the monitoring period yet it accumulated only sparingly in the lagoons, mainly around the inlet of the primary lagoon. The estimated per capita sludge accumulation rate was 0.0047 m³/person/year. The total capital costs of the scheme were £1.65 million, and of this the lagoon cost was £840 000. The capital costs of £420 per capita make this more expensive than other, similar options, but plant operating costs are significantly reduced.     

The persistence and removal of enteric pathogens in constructed wetlands

Sedimentation is thought to be one of the mechanisms of microbial reduction from wetlands used for wastewater treatment. This study compared the occurrence and survival of enteric indicator microorganisms and pathogens in the water column and sediments of two constructed surface flow wetlands in Arizona. On a volume/wet weight basis the concentration of fecal coliforms and coliphage in the water column and sediment was similar. However, on a volume/dry weight basis the numbers were one to two orders of magnitude higher in the sediment. Giardia cyst and Cryptosporidium oocyst concentrations were one to three orders of magnitude greater in the sediment compared to the water column. The die-off rates of all the bacteria and coliphage were greater in the water column than the sediment. The die-off rates of fecal coliforms in the water and sediment were 0.256log(10)day(-1) and 0.151log(10)day(-1), respectively. The die-off rates of Salmonella typhimurium in the water and sediment were 0.345log(10)day(-1) and 0.312log(10)day(-1), respectively. The die-off rates of naturally occurring coliphage in water column and sediment were 0.397log(10)day(-1) and 0.107log(10)day(-1), respectively, and the die-off rates of and PRD-1 in water and sediment were 0.198log(10)day(-1) and 0.054log(10)day(-1), respectively. In contrast Giardia die-off in the sediment was greater compared to the water column. The die-off rates of Giardia in water and sediment were 0.029log(10)day(-1) and 0.37log(10)day(-1), respectively. Coliphage survived the longest of any group of organisms in the sediment and the least in the water column. In contrast Giardia survived best in the water column and least in the sediment.     

Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography/mass spectrometry

Approximately one-half of the 50,000,000 lb of antibiotics produced in the USA are used in agriculture. Because of the intensive use of antibiotics in the management of confined livestock operations, the potential exists for the transport of these compounds and their metabolites into our nation's water resources. A commercially available radioimmunoassay method, developed as a screen for tetracycline antibiotics in serum, urine, milk, and tissue, was adapted to analyze water samples at a detection level of approximately 1.0 ppb and a semiquantitative analytical range of 1-20 ppb. Liquid waste samples were obtained from 13 hog lagoons in three states and 52 surface- and ground-water samples were obtained primarily from areas associated with intensive swine and poultry production in seven states. These samples were screened for the tetracycline antibiotics by using the modified radioimmunoassay screening method. The radioimmunoassay tests yielded positive results for tetracycline antibiotics in samples from all 13 of the hog lagoons. Dilutions of 10-100-fold of the hog lagoon samples indicated that tetracycline antibiotic concentrations ranged from approximately 5 to several hundred parts per billion in liquid hog lagoon waste. Of the 52 surface- and ground-water samples collected all but two tested negative and these two samples contained tetracycline antibiotic concentrations less than 1 ppb. A new liquid chromatography/mass spectrometry method was used to confirm the radioimmunoassay results in 9 samples and also to identify the tetracycline antibiotics to which the radioimmunoassay test was responding. The new liquid chromatography/mass spectrometry method with online solid-phase extraction and a detection level of 0.5 microg/l confirmed the presence of chlorotetracycline in the hog lagoon samples and in one of the surface-water samples. The concentrations calculated from the radioimmunoassay were a factor of 1-5 times less than those calculated by the liquid chromatography/mass spectrometry concentrations for chlorotetracycline.     

Temporal flux and spatial dynamics of nutrients, fecal indicators, and zoonotic pathogens in anaerobic swine manure lagoon water

Confined animal feeding operations (CAFOs) often use anaerobic lagoons for manure treatment. In the USA, swine CAFO lagoon water is used for crop irrigation that is regulated by farm-specific nutrient management plans (NMPs). Implementation of stricter US environmental regulations in 2013 will set soil P limits; impacting land applications of manure and requiring revision of NMPs. Precise knowledge of lagoon water quality is needed for formulating NMPs, for understanding losses of N and C in ammonia and greenhouse gas emissions, and for understanding risks of environmental contamination by fecal bacteria, including zoonotic pathogens. In this study we determined year-round levels of nutrients and bacteria from swine CAFO lagoon water. Statistical analysis of data for pH, electrical conductivity (EC), inorganic and organic C, total N, water-soluble and total minerals (Ca, Cu, Fe, K, Mg, Mn, P, and Zn) and bacteria (Escherichia coli, enterococci, Clostridium perfringens, Campylobacter spp., Listeria spp., Salmonella spp., and staphylococci) showed that all differed significantly by dates of collection. During the irrigation season, levels of total N decreased by half and the N:P ratio changed from 9.7 to 2.8. Some seasonal differences were correlated with temperature. Total N and inorganic C increased below 19 °C, and decreased above 19 °C, consistent with summer increases in ammonia and greenhouse gas emissions. Water-soluble Cu, Fe, and Zn increased with higher summer temperatures while enterococci and zoonotic pathogens (Campylobacter, Listeria, and Salmonella) decreased. Although their populations changed seasonally, the zoonotic pathogens were present year-round. Increasing levels of E. coli were statistically correlated with increasing pH. Differences between depths were also found. Organic C, total nutrients (C, Ca, Cu, Fe, Mg, Mn, N, P, and Zn) and C. perfringens were higher in deeper samples, indicating stratification of these parameters. No statistical interactions were found between collection dates and depths.     

The impact of hourly solar radiation model on building energy analysis in different climatic regions of Turkey

The purpose of this study is to investigate the effect of solar radiation models on the determination of energy performance of a single-family house assisted with renewable energy system including photovoltaic panels and solar water heater. An Angström-Prescott type solar radiation model was compared with Zhang and Huang model derived based on hourly meteorological data of 12 locations in Turkey. Since regression coefficients of the Zhang and Huang model are valid for China, new regression coefficients were derived by using local meteorological data. A clear distinction could not be observed in simulated annual heating load intensity for each model since the average relative deviation of the models’ results was 2.5%. However, the average deviation was 12.5% for space cooling load intensity. Primary energy ratings (PER) and the renewable energy ratio (RER) were determined for each location. For total PER, the highest deviation was 4.6% and 3.3% for Mersin and Mu?la, respectively. For the other locations, this parameter deviates between 0.02%–2.11%. The highest RER was 18.6% for Mersin.     

Bacterial contamination of Mediterranean coastal seawater as affected by riverine inputs: simulation approach applied to a shellfish breeding area (Thau lagoon,France)

Consequences of short-term changes in thermotolerant coliform loads on their spatio-temporal distribution in a Mediterranean lagoon with large-scale mollusk farming (Thau lagoon, France) were explored using a simulation approach. Simulations were based on bacterial transport and survival coupled models forced by the input of bacterial loads from the two main rivers (Vène and Pallas) that flow into the lagoon. Different flow types (reference, sudden and constant), bringing the same bacterial load, were considered and subsequent spatial and temporal bacterial contamination of lagoon surface water and shellfish was estimated. Simulation results showed that as long as loads were high, hydrodynamical processes governed the distribution of bacterial abundance in receiving areas. As soon as loads decreased or when time supply increased, biological die-off processes became dominant. Bacterial contamination of shellfish induced by the different flow types appeared to depend on the receiving area. In the case of Pallas River area, a sudden input of bacteria led to a high bacterial contamination of shellfish but only during a short period ( approximately 1 day). A constant input of the same amount of bacteria induced a lower but significant contamination during all the simulation period (10 days). On the contrary, bacterial inputs from the Vène River led to shellfish contamination only when bacteria were delivered through a flood event. Exposure time of bacteria to adverse environmental conditions appeared to be the main explanation to the above-mentioned differences. Consequences of our results in terms of environmental management strategy were discussed.     

Application of HDPE at a sewage treatment plant

A pair of aeration lagoons at a wastewater treatment facility were lined with 0.75-mm HDPE geomembrane in 1993. The ponds were placed in an area that previously contained an unlined sewage stabilization pond. This paper discusses activity at the east lagoon only. Prior to liner installation, a gas vent system was added in the design in the event that gases formed under the liner. That system employed approximately 30-cm-wide strips of geotextile/geonet geocomposite placed on 30-m centers in a grid pattern. Gas vents were cut in the liner around the top perimeter of the pond at 15-m centers. Thorough construction quality assurance practices were employed to insure that the geomembrane and seams were of a high quality. Seven months after installation, gas bubbles began to form under the liner, and large bubbles resulted. The bubbles were pierced to remove the gas. At that time, the lagoon was taken out of service and drained for inspection. Crescent moon-shaped cuts (about 300 mm in size) were then found at the location of some of the baffle curtain anchors. This damage was repaired in conjunction with the holes created by venting the gas bubbles. The lagoon was then partially filled, and more bubbles were formed under the liner. Analysis of the gas resulted in the opinion that it was the result of anaerobic digestion of organic matter under the liner. Also found were holes approximately 60 mm in size that were grouped in random locations throughout the lagoon. These holes were in pairs and spaced 3 to > 30 mm apart. The source of those holes remains unknown. In retrospect, a gas vent system capable of removing gases at a greater rate than it was generated from beneath the liner was required. It is suggested that when a previously existing pond is to be lined, extensive testing for organic matter be performed on subgrade material. Such testing is not currently well defined and should occur prior to design. Other investigations may include hydrological surveys that include several years' data on underlying water level activity. Also, a thicker geomembrane may have had enough resistance to mechanical damage to prevent the formation of the crescent moon and smaller holes.     

Coliform bacteria from diffuse sources as a factor in estuarine pollution

The contribution of 849 ha of rural watershed to the fecal coliform (FC) pollution of the Rhode River, a subestuary of Chesapeake Bay with a surface area of 485 ha. was estimated. The watershed with an animal population of 0.6 animal unit ha⁻¹ discharged between 7.5 × 10⁶ and 669 × 10⁶ FC ha-day⁻¹. The FC discharge rate was seasonal and largely dependent upon the water flow. Total coliform (TC) discharge was influenced by the same factors as the FC discharge. It was calculated that on the average less than 1% of FC produced by the animals on the land was washed down by water runoff. Occasionally the FC discharge rate in the runoff reached 4–6% of the FC produced by the animals. Fecal coliforms persisted in the water. The FC numbers were high in the Rhode River close to the discharge points and further away were diluted by the river volume. It was estimated, using the maximum number of FC in the runoff, that 2600 m³ of well mixed receiving water was needed for every ha of watershed area not to exceed the safe water standards (14 FC MPN 100 ml⁻¹ for shellfish harvesting. Three factors having a role in FC pollution of an estuarine ecosystem were emphasized as a result of this study: (1) the rural watershed contributed substantial quantities of FC to the estuary; (2) the season of the year may determine the level of pollution entering from rural sources into the estuary; (3) the persistence of bacteria in the estuary may increase the pollution level contributed by the watershed especially at low water temperatures.     

Calibrating and validating bacterial water quality models: A Bayesian approach

Water resource management decisions often depend on mechanistic or empirical models to predict water quality conditions under future pollutant loading scenarios. These decisions, such as whether or not to restrict public access to a water resource area, may therefore vary depending on how models reflect process, observation, and analytical uncertainty and variability. Nonetheless, few probabilistic modeling tools have been developed which explicitly propagate fecal indicator bacteria (FIB) analysis uncertainty into predictive bacterial water quality model parameters and response variables. Here, we compare three approaches to modeling variability in two different FIB water quality models. We first calibrate a well-known first-order bacterial decay model using approaches ranging from ordinary least squares (OLS) linear regression to Bayesian Markov chain Monte Carlo (MCMC) procedures. We then calibrate a less frequently used empirical bacterial die-off model using the same range of procedures (and the same data). Finally, we propose an innovative approach to evaluating the predictive performance of each calibrated model using a leave-one-out cross-validation procedure and assessing the probability distributions of the resulting Bayesian posterior predictive p-values. Our results suggest that different approaches to acknowledging uncertainty can lead to discrepancies between parameter mean and variance estimates and predictive performance for the same FIB water quality model. Our results also suggest that models without a bacterial kinetics parameter related to the rate of decay may more appropriately reflect FIB fate and transport processes, regardless of how variability and uncertainty are acknowledged.     

Inverse estimation of nonpoint sources of fecal coliform for establishing allowable load for Wye River, Maryland

An estimation of the nonpoint sources of fecal coliform (FC) has been formulated as an inverse parameter estimation problem, using FC observation data from the receiving waters. An inverse algorithm was integrated into the three-dimensional transport model, HEM-3D. The modified Gauss-Newton method was used for the optimal estimation of the loads. The approach is straightforward and requires less effort in modification of the existing hydrodynamic model. Model experiments were conducted to investigate the feasibility of load estimation in a tidal river. The parameter uncertainty and accuracy associated with load estimation are discussed using data sets with different sampling sizes. The inverse model was applied to the tidal Wye River, Maryland, USA, to estimate FC nonpoint sources and to develop an allowable load for the river to attain water quality standards. The model results suggest that the inverse modeling approach is suitable for estimating FC nonpoint sources. The inverse method not only provides an efficient approach to assess the contribution of nonpoint sources, but has the additional advantage of addressing the problems of the uncertainty and error associated with FC simulation in the estuary using hydrodynamic models.