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1.
压电基因传感器是一种新型的生物传感器,它把压电传感器的灵敏性和DNA杂交反应相结合.与传统的基因检测技术相比,它具有结构简单、无需标记、检测时间短、检测信号易处理等特点.将它用于分子运算,与常规的DNA芯片相比,它的检测结果更易于进行自动化处理,因此便于构建大规模的分子运算机器.文中在压电基因传感器和新兴学科DNA计算的基础上,给出了解决0-1规划问题新的DNA计算方法,并指出以前两种基于表面DNA计算在解决这一问题时的不足.与以往的DNA计算方法相比其输出的是电信号,因此具有操作易自动化、识别解更方便和高信息量的优点.与使用常规DNA芯片的表面DNA计算相比,使用压电基因传感器进行DNA计算可以克服可行解识别困难的问题.压电基因传感器技术有望成为新的分子运算工具,可作为构建自动化的DNA计算机的基础.  相似文献   

2.
In recent years, several strategies for DNA based molecular computing have been investigated. An important area of research is the detection and analysis of output molecules. We demonstrate how DNA computing can be extended with in vivo translation of the output. In the resulting proteins, the information per kilogram is about 15-fold higher than in the original DNA output. The proteins are therefore of correspondingly smaller mass, which facilitates their subsequent detection using highly sensitive mass spectrometry methods. We have tested this approach on an instance of the Minimal Dominating Set problem. The DNA used in the computation was constructed as an open reading frame in a plasmid, under the control of a strong inducible promoter. Sequential application of restriction endonucleases yielded a library of potential solutions to the problem instance. The mixture of plasmids was then used for expression of a protein representation. Using MALDI-TOF mass spectrometry, a protein corresponding to the correct solution could be detected. The results indicate the feasibility of the extension of DNA computing to include protein technology. Our strategy opens up new possibilities for both scaling of DNA computations and implementations that employ output of functional molecules or phenotypes.  相似文献   

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Current methods used for detection of DNA hybridization involve the use of DNA microarrays which require overnight incubation times along with bulky and expensive fluorescent scanners. Here, we demonstrate electrical detection of DNA hybridization in an oligonucleotide functionalized microfluidic channel. We use microchannels functionalized with DNA probes integrated with electrodes for measuring conductance across the channel. As beads conjugated with the target DNA passing through the channel are captured on the surface, we are able to electrically detect changes in resistance due to bead capture. Our assay can be completed in less than an hour using less than a microliter of reagent, and has the potential for extensive multiplexing. Such a device can be useful as a handheld platform in a clinical setting where one would need to rapidly genotype a small number of genes rapidly.  相似文献   

5.
ABSTRACT

In this paper, we propose a novel intrusion detection approach using past driving experience and the neural knowledge DNA for in-vehicle information system security. The neural knowledge DNA is a novel knowledge representation method designed to support discovering, storing, reusing, improving, and sharing knowledge among machines and computing systems. We examine our approach for classifying malicious vehicle control commands based on learning from past valid driving behavior data on a simulator.  相似文献   

6.
The concept of aqueous computing involves the use of large numbers of initially identical molecules to serve as memory registers in a fluid environment. Here, we test a new approach to aqueous computing where modified nucleotides are used to “write” on double-stranded DNA molecules to establish the logical values of true or false for a set of clauses. We introduce an implementation scenario where binding proteins specific to each modification can be used to selectively isolate DNA fragments with these modified nucleotides. In addition, we present initial results showing successful incorporation and detection of modifications. We have successfully labeled DNA fragments with four modifications, specifically Alexa Fluor-488, BODIPY-FL, biotin, and digoxigenin using polymerase chain reaction. The first two produce fluorescent molecules that can be distinguished by their color. We have confirmed that binding proteins or antibodies to these four modifications are specific and do not detect the other modifications. We have also successfully separated the DNAs labeled with Alexa Fluor and biotin using binding proteins. We present attempts at rebinding these modified molecules to a second binding protein; the equivalent of applying more than one clause to a set of values. We have found some challenges with this approach that likely can be resolved with further work. As there are millions of molecules with corresponding binding proteins, this approach has the potential to yield unlimited computing power as compared with other aqueous computing methods.  相似文献   

7.
DNA计算因其优异的计算能力已经成为当前研究热点,DNA逻辑计算模型是DNA计算体系与运算实现的重要依托。按应用技术将现有DNA逻辑计算模型进行分类:基于链置换的DNA逻辑计算模型、基于核酶的DNA逻辑计算模型、基于G-quadruplex的DNA逻辑计算模型、基于DNA自组装的逻辑计算模型、基于其他分子技术和分子材料的DNA逻辑计算模型。首先阐述了DNA逻辑计算的研究背景和研究目的以及现阶段在生物分子检测、疾病诊断、多因素分析和生物成像等领域的应用并简述其相关概念;然后梳理各DNA逻辑计算模型的研究历史和现状,分析各类逻辑计算模型所应用的分子操控技术和分子材料以及优缺点和应用前景;最后归纳总结DNA逻辑计算领域当前研究热点和发展前景,为未来提出全新的计算方式奠定基础,为信息、医疗等领域提供更好的服务。  相似文献   

8.
在DNA序列相关物理特性的模拟计算中,计算时间随DNA比较序列数的增加而延长。该研究成功应用分布式运算实现了对DNA序列间相关物理特性的模拟计算。该研究表明:对较大的DNA序列进行比较时,使用JavaParty和增加线程数能有效地缩短运行时间。  相似文献   

9.
求解0-1规划问题的DNA计算模型(英文)   总被引:1,自引:0,他引:1  
DNA计算是以DNA分子作为数据的一种新型计算模式.在DNA计算中首要面对的问题是编码问题.文中提出了一种双编码方法,利用这种编码方法可以使得在DNA计算的读解过程类似于DNA测序过程,容易实现自动化操作.基于该编码方法所建立的DNA计算模型可用于求解0-1规划问题,只需4次PCR反应即可读取问题的可行解.与其他DNA计算模型相比,该模型具有操作简单、易于实现的优点.  相似文献   

10.
DNA计算是以DNA分子作为数据的一种新型计算模式.为了减少DNA计算中编码的数量,不降低生化实验操作的可靠性,文中建立了一种基于酶切技术和PCR技术的图顶点着色DNA计算模型,给出了实现该模型的双编码的编码方案.分析表明,利用酶切技术和PCR技术能够有效删除非解并读取真解.该模型的解的检测方法类似于DNA测序技术,使得该模型更容易实现自动化操作.  相似文献   

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