共查询到20条相似文献,搜索用时 562 毫秒
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分子印迹技术是制备具有分子特异识别功能聚合物的一种技术。分子印迹固相萃取技术由于具有高通量和高特异性,在食品、环境、药物、生物样品中分析物的分离、净化、富集中具有广阔的应用前景。文章中综述了分子印迹固相萃取技术的应用、分子印迹技术的最新进展和在应用中存在的问题等。 相似文献
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天然产物资源丰富,其高效利用离不开先进的分离技术。兼具离子液体高选择性及聚合物安全、易回收等特性的聚离子液体为天然产物的分离提供了新途径。在介绍聚阳离子型、聚阴离子型和聚两性型离子液体制备技术的基础上,综述了使用聚离子液体的固相萃取、固相微萃取、分子印迹固相萃取、液液萃取及毛细管电泳技术在黄酮类、生物碱类、酚类、蛋白质类等天然产物分离中的研究进展,分析了聚离子液体分离作用机理及其具有良好的分离效率、稳定性和回收再利用性能的原因,特别强调了刺激响应聚离子液体在天然产物分离中的优势,并从聚离子液体设计和定制出发对聚离子液体在分离领域所存在的挑战和潜在的应用进行了展望。 相似文献
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Ralf Thiericke 《Journal of Automated Methods and Management in Chemistry》2000,22(5):149-157
Secondary metabolites from plants, animals and microorganisms have been proven to be an outstanding source for new and innovative drugs and show a striking structural diversity that supplements chemically synthesized compounds or libraries in drug discovery programs. Unfortunately, extracts from natural sources are usually complex mixtures of compounds:: often generated in time consuming and for the most part manual processes. As quality and quantity of the provided samples play a pivotal role in the success of high-throughput screening programs this poses serious problems. In order to make samples of natural origin competitive with synthetic compound libraries, we devised a novel, automated sample preparation procedure based on solid-phase extraction (SPE). By making use of a modified Zymark RapidTrace® SPE workstation an easy-to-handle and effective fractionation method has been developed which allows the generation of highquality samples from natural origin, fulfilling the requirements of an integration into high-throughput screening programs. 相似文献
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The response surface methodology was used to evaluate the effects of extraction time, power of ultrasound, liquid to solid ratio, and solvent composition on the quantity and quality (from aspect of antioxidant activity) phenolics of Stachys lavandulifolia. The best extraction time, power of ultrasound, liquid to solid ratio, and solvent composition for both the quality and quantity of phenolics were 14 min, 300 W, 40 (v/w), and 57% methanol, respectively. Only the liquid to solid ratio was effective on the quality of phenolics. Also, the comparison between the ultrasound-assisted extraction and maceration methods showed the suitability of ultrasound-assisted extraction for extracting phenolics from this plant. 相似文献
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C Dufresne 《Journal of Automated Methods and Management in Chemistry》2000,22(6):175-179
Nature has always been a productive source of new drugs. With the advent of high-throughput screening, it has now become possible to rapidly screen large sample collections. In addition to seeking greater diversity from natural product sources (micro-organisms, plants, etc.), fractionation of the crude extracts prior to screening is becoming a more important part of our efforts. As sample preparation protocols become more involved, automation can help to achieve and maintain a desired sample throughput. To address the needs of our screening program, two robotic systems were designed. The first system processes crude extracts all the way to 96-well plates, containing solutions suitable for screening in biological and biochemical assays. The system can dissolve crude extracts, fractionate them on solid-phase extraction cartridges, dry and weigh each fraction, re-dissolve them to a known concentration, and prepare mother plates. The second system replicates mother plates into a number of daughter plates. 相似文献
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Automated fluorescence microscopy provides a powerful tool for analyzing the physiological state of single cells with high throughput and high information content. Here I discuss two types of experiments in which this technology was used to discover and characterize bioactive small molecules. In phenotypic-screening experiments, the goal is to find "hits" with specific effects on cells by screening large libraries of small molecules. An example is screening for small molecules that perturb mitosis by novel mechanisms. In cytological-profiling experiments, the goal is to characterize the bioactivity of a limited number of small molecules in considerable depth, and thus understand their mechanism and toxicities at the cellular level. I discuss an example in which 100 small molecules with known bioactivity were profiled by using multiple fluorescent probes, and clustered into mechanistic classes by automated statistical analysis. 相似文献
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简要叙述了天然有机物提取与分离方法,如超临界流体萃取技术,超声波提取技术,微波提取技术,酶提取和仿生提取技术,固相萃取和固相微萃取技术技术.对高数逆流色谱技术、大孔树脂吸附法技术进行了详细的描述. 相似文献