中国重要海洋中上层经济鱼类生物学研究进展

中国海洋中上层鱼类的种类繁多,近几年来,其总产量占全国海洋捕捞总产量的40%左右。日本鲭、蓝圆鲹和鳀等中上层鱼类生活史大多为r选择型,资源更新速度一般比底层和近底层鱼类较快,因此其资源可持续利用的前景更为广阔。本文综述了中国重要海洋中上层鱼类渔业的发展概况,主要介绍了年产量位于前列的日本鲭、蓝圆鲹、蓝点马鲛、银鲳和鳀等5种鱼类生物学的研究进展,阐明了这些种类的种群鉴别和划分、洄游分布、越冬场、产卵场和产卵期、年龄和生长、摄食习性、生殖力和群体结构变化等资源生物学的研究现状,以及其资源量和可捕量的评估结果,并展望了其资源生物学研究的前景。     

中国主要海洋底层鱼类生物学研究的回顾与展望

回顾了中国海洋主要底层经济鱼类资源生物学的研究历史和科研成果,并重点叙述了带鱼、小黄鱼、大黄鱼和绿鳍马面鲀等重要经济鱼类资源生物学研究概况.概述的内容包括地理种群及其产卵群体的鉴别和划分、生活史型的演变、种群和群体结构、种群数量变动、年龄组成和生长特性、摄食习性、性成熟周期、性腺成熟指数(GSI)、产卵群体生殖力、产卵场、索饵场、越冬场及洄游路线、资源量和渔获量、各种作业渔船的调整及其捕捞力量的限制措施等,并展望了中国海洋底层经济鱼类资源生物学研究的前景.     

从鱼类区系和中上层鱼类生物学特性看我国海洋渔业发展的前景

我国近海各海区由于海洋环境不同，鱼类区系及其生物学特性迥异，鱼类资源潜力也不一致。近年来某些底层鱼类资源呈现衰退现象，我国海洋渔业发展的前景如何，是众所关心的一个大问题。本文试图从我国近海鱼类区系和中上层鱼类生物学特性来探讨我国海洋渔业发展的前景，不妥之处，请批评指正。     

黄河入海泥沙年际通量对莱州湾渔业资源结构的影响

根据2009年8月-2013年8月莱州湾海域渔业拖网调查数据,分析该海域渔业种群组成、优势种、空间分布和资源量的年际变动,以了解黄河入海泥沙通量对莱州湾渔业结构的影响.结果表明:(1)莱州湾渔业资源量整体呈下降趋势,主要以鱼类为主,甲壳类次之,头足类最少;鱼类中以中上层鱼类为主,网获量明显多于底层鱼类,但2011年8月底层鱼类稍多于中上层鱼类;(2)莱州湾渔业资源各年份的优势种有所变动,中上层鱼类和底层鱼类的优势种变动较大,甲壳类优势种变动较少,鱼类优势种组成存在年际更替现象,主要由经济价值高、个体大的种类向经济价值低、个体小的种类演替.主要优势种集中于斑鲦、矛尾鰕虎鱼、口虾蛄、小带鱼和日本枪乌贼等种类.2010年斑鲦大量暴发,为唯一优势种;(3)2009-2013年黄河入海泥沙年际通量与鱼类种数、底层鱼类数和渔业资源数存在显著相关性,与底层鱼类生物量存在极显著相关性.综上所述,黄河入海泥沙年际通量的变动对莱州湾渔业资源量和资源结构都产生了一定影响.     

小型中上层海洋鱼类资源评估研究进展

小型中上层海洋鱼类是重要的渔业资源,目前其捕捞产量约占到全球海洋渔获量二分之一。小型中上层海洋鱼类具有生命周期短、生长速度快、分布不均匀、易受环境因素影响等生物学特点,近年来,一些小型中上层海洋鱼类渔获量出现下降趋势,为保证其资源的可持续利用,对其进行准确的资源评估研究和制定合理有效的管理策略显得尤为重要。以文献计量统计分析为基础,对20多年来应用于小型中上层海洋鱼类资源评估的模型方法以及所需数据类型进行归纳与回顾,同时对模型中重要的参数估计、不确定性来源进行总结。分析认为,由于缺乏完整、准确的生物学信息导致无法对小型中上层海洋鱼类使用传统的资源评估方法进行评估,因此,其资源评估研究仍处在发展阶段。建议今后研究中应开展以下工作:1)努力提高现有模型的评估精度,尽可能考虑更多影响因素; 2)要进行长期系统的渔业资源独立调查; 3)充分利用体长等易获得数据,开发体长结构模型和基于生态系统的评估模型,降低模型选择的局限性。     

渔业动态

日本水产厅所属的日本最大的渔业资源调查船开洋丸(2,630t级),为了2003年9月初召开的日本全国资源评估会作出2004年度的ABC(生物学的容许渔获量)收集有关基础数据,继2003年春在西北太平洋对中上层鱼类的太平洋种群资源进行了调查的基础上,为了进一步摸清中上层鱼类从越冬场到索饵场北上洄游期间的资源状     

2006年春、秋季黄海山东海域鱼类资源结构与数量分布

为了解山东近海渔业资源结构及数量分布现状,2006年春季(5月)和秋季(10月)利用疏目变水层双拖网对黄海山东海域进行了调查.数据分析结果表明,春季近岸浅水区资源量指数明显高于远岸深水区,以海州湾资源量指数最高；秋季,近岸浅水区资源量指数仍稍高于深水区,资源量指数以调查海域南部中间区域最低而海州湾最高.春、秋季调查各捕获鱼类50种.基于生物量的分析表明,按经济价值结构划分,春、秋季均以经济价值较低种类为主；按栖息水层划分,春季以底层鱼类为主,秋季以中上层鱼类为主；按适温类型结构划分,春季以冷温性种类为主,秋季以暖温性种类为主.春季生物量最高的3个种类为玉筋鱼(Ammodytes personatus)、鳀(Engraulisjaponicus)和方氏锦鳚(Enedrias fangi),秋季为鳀、青鳞小沙丁鱼(Sardinella zunasi)和玉筋鱼.春、秋季标准生物量谱的斜率均小于-1,表明种群生物量随着个体质量的增加而减少.鱼类资源组成以小型种类为主,春季鱼类平均体质量为2.36 g,秋季为3.34 g.春季平均个体质量小于10g的种类合计生物量占总生物量的95.74％,秋季则为82.09％.与20世纪80年代相比,本次调查期间鱼类的经济结构更加低质化；资源衰退的趋势虽有所遏制,但渔业资源尤其是传统经济种类资源并未根本恢复；优势种类更替明显,玉筋鱼的优势地位明显上升.根据研究结果认为,应将伏季休渔期提前.     

闽南-台湾浅滩渔场鱼类及其不同生态类群的资源生产量

以闽南-台湾浅滩渔场历次海洋科学调查获得的初级生产力为基础,在对该渔场的鱼类资源结构调查、浮游植物有机碳含量检测和生态效率的测算、52种主要经济鱼类的营养级及其有机碳含量的检测后,先采用营养动态模型和Cusing模型估算鱼类资源的生态容量(潜在生产量),然后应用Seetile模型分别估算中上层鱼类、底层鱼类的资源生产量,进而从鱼类资源生产量中分离出近底层鱼类的资源生产量,采用Gadima模式和MSY简单模式估算各生态类群的鱼类资源最大可持续开发量,并讨论它们的开发利用程度.估算结果:鱼类资源生态容量为98.62×104 t,最大持续可开发量为48.34×104 t.其中中上层鱼类资源生产量为56.90×104 t,最大持续可开发量为28.35×104 t,底层和近底层鱼类资源生产量分别为19.45×104 t和22.27×104 t,合计最大持续可开发量为21.27×104 t.底层和近底层鱼类实际年渔获量自1994年以来已连续9年超过其最大持续可开发量,呈过度捕捞.中上层鱼类实际年渔获量至今尚未超过其最大可持续开发量.     

巴基斯坦伊斯兰共和国沿海渔场及渔具渔法的探讨

通过一个生产周期的调研,基本上掌握了巴基斯坦沿海渔场的分布情况。对主要捕捞对象生物学、渔期、渔汛的规律有了一定的了解,为指导捕捞生产起了很大的作用。使用网目尺寸25cm和1.5m网具的适渔性较强,捕捞效率也高。巴基斯坦沿海底渔资源开发力度较大,中上层及外海鱼类资源具有开发前景。     

闽南、台湾浅滩渔场中上层鱼类群聚资源动态

本文根据近10年来在闽南、台湾浅滩渔场开展的中上层鱼类资源动态监测资料，报道了该渔场中上层鱼类资源利用现状及其群聚动态变化情况，并就今后如何利用该资源进行探讨，为其合理开发利用提供科学依据。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.