海洋假单胞菌SB12产表面活性剂的研究

从汕头湾海底沉积物中分离到24株表面活性剂产生菌,对其中一株产生物表面活性剂能力最强的菌株(SB12)进行鉴定、发酵条件优化及生物表面活性剂特性的初步研究.经鉴定,确定该菌株为假单胞菌(Pseudomonas sp.).对其产生物表面活性剂的发酵条件进行优化,确定了最佳单因子发酵条件:氮源为蛋白胨,盐浓度为0.1%,pH 9.5,温度为25℃,培养时间为6 d;优化后的最小表面张力为19.77 mN/m.分析发酵液中生物表面活性剂的特性发现:菌株SB12产生的生物表面活性剂具有良好的乳化性能,乳化力达91%;产生的生物表面活性剂具有较广的温度和pH适应范围.     

黑木耳菌糠基质下毛木耳细胞工程菌株的生殖生长

以毛木耳［Auricularia polytricha(Mont.)Sacc］细胞工程菌株新河大SL205为供试菌株,以黑木耳菌糠为主要生殖生长基料,以菌丝长速、生殖生长生物学效率为指标,设计不同培养基配方进行实验.结果表明:供试菌株新河大SL205的适宜培养基配方为黑木耳菌糠50%(质量分数,下同)、木屑30%、玉米芯10%、麸皮8%、石灰2%;该培养基下,新河大SL205平均长速为7.26 mm/d,生物学效率为126.29%,对黑木耳菌糠生物降解能力最强,有效降低了黑木耳菌糠带来的面源污染.     

单核细胞增多性李斯特菌对人、鼠脑微血管内皮细胞黏附、侵袭、增殖差异性分析

为探究单核细胞增多性李斯特菌(LM)不同菌株对脑微血管内皮细胞黏附,侵袭以及在细胞内增殖的差异,本实验利用LM90SB2、LM3、LM4,塞氏李斯特菌(L.seeligeri),无害李斯特菌(L.innocua)感染脑微血管内皮细胞,采用BHI平板计数,测定细菌黏附率、侵袭率及不同时间细胞内增殖情况。结果表明:LM90SB2、LM3、LM4对脑微血管内皮细胞黏附率为3.14%~7.83%,统计学分析其差异不显著;LM90SB2、LM3、LM4对人/鼠脑微血管内皮细胞侵袭率(0.036%~0.128%)均大于L.seeligeri(0.0315%、0.00148%),L.innocua不侵袭脑微血管内皮细胞。LM90SB2、LM3、LM4对人/鼠2种细胞的侵袭率存在显著差异(P0.05),临床分离株LM90SB2增殖速度、菌量及持续时间明显高于其它菌株。以上结果提示LM不同菌株的毒力不同。     

龙虎泡油田微生物驱油评价实验

 为了在大庆龙虎泡油田应用微生物采油技术, 进行室内评价实验。从龙虎泡油田地层水中筛选到一株内源菌DH8, 经鉴定为Geobacillusstearothermophilus, 另外从菌种库中挑选一株适合该油藏条件生长的外源菌SL21, 经鉴定为Bacillus licheniformis。室内对DH8 和SL21 的乳化性能、降解能力和驱油效率进行了评价。研究发现, 菌株DH8 和SL21 发酵液的比毛管数分别为0.0239 s/m 和0.0332 s/m; 菌株DH8 和SL21 分别使原油黏度降低19.02%和26.08%; 气相色谱分析表明菌株DH8 主要降解C₁₃~C₂₇内正构烷烃, 而菌株SL21 主要降解C₁₉~C₃₇内正构烷烃, 经DH8 和SL21 降解后, C₁₃之前烷烃含量占比分别增加了5.33%和11.00%; 物模实验表明, 菌株SL21 和菌株DH8 的驱油效率分别为7.11%和5.50%。综合分析得出外源菌SL21 的各项驱油性能指标均优于内源菌DH8。     

一株富含类胡萝卜素光合细菌的分离鉴定

从不同生物环境中分离紫色非硫菌,测定其类胡萝卜素含量,筛选出富含类胡萝卜素的菌株.对该菌株的形态学特征、碳氮源利用情况、最适生长pH值及盐度等生物学特征进行研究;利用PCR技术扩增其16S rRNA基因,测序后进行16S rRNA基因序列分析,研究其分类地位.结果:分离自暨南大学明湖底泥的菌株R2,在所分离的8株紫色非硫菌中,类胡萝卜素质量分数最高,为4.83 mg/g(干菌体),该菌株符合红游动菌属(Rhodoplanes)的生物学特性,其最适生长pH为7.0,盐度为0.2%;16S rRNA基因的分子系统学分析表明,该菌株与红游动菌属的Rhodoplanessp.HA17相似性为99.4%.可确定筛选出一株高产类胡萝卜素的菌株,该菌株属于红游动菌属(Rhodoplanes).     

SL益生素生产菌乳链球菌若干特性

乳链球菌ＳＢ９００为“ＳＬ益生纱生产菌，对其体内外若干特性进行研究，结果表明：该菌株产酸力强，乳酸产量为１．７３％；耐酸性能好，在ｐＨ２的盐酸溶液中，３７℃保温１ｈ，存活率为４６．６％；具有一定的抑菌作用并不受胃蛋白酶和胰蛋白酶的影响；对临床上治疗腹泻的常用药有耐受性；胆汁能促进其生长，喂以该菌制剂的鸡肠道中，服酸菌数量增加，Ｅ．ｃｏｌｉ减少。     

浮游球衣菌的分离及其对铅离子的吸附性能

利用改进后的菌种分离方法,从某污水中分离得到一菌株·研究了该菌株的菌落形态特征、菌株形态特征、生理生化特性、生长曲线、菌种保藏方法及其对Pb2+的吸附特性·经研究确定该菌株为第十四群鞘细菌类(sheathedbacteria)球衣菌属(Sphaerotilus)浮游球衣菌(Sphaerotilusnatans)·吸附实验结果表明:浮游球衣菌对Pb2+有很好的吸附效果,在10min内即可达到吸附平衡;在pH值约为5 5,菌的质量浓度为0 6g/L,铅离子初始质量浓度不大于20mg/L时,Pb2+去除率接近100%·     

小麦根系联合固氮菌筛选与回接作用

从我国河北省农田小麦根系上分离出三株联合固氮菌W—43、W—432、W—91。对其细胞形态、培养特征、生理生化特性及DNA中G+C含量的研究结果表明,W—43菌株为肺炎克氏杆菌(Klebsiella pneumoniae)、W—432菌株为产气肠杆菌(En-terbacter aerogenes)、W—91菌株为粪产碱菌(Alcaligenes faecalis)。利用这三株菌回接小麦播种,促进其生长发育,改善氮素营养状况,增加生物学产量6.5—11.7％。     

可产木聚糖酶的蕙兰根内生细菌的筛选与鉴定

为了获得可产木聚糖酶的功能菌,通过刚果红染色法对592株蕙兰(Cymbidium faberi)根内生细菌进行初筛,DNS法对初筛获得的菌株进行复筛,并通过形态学、生理生化特性、(G+C)的物质的量分数及16SrRNA基因序列分析对菌株进行初步鉴定.结果筛选出31株可产木聚糖酶的菌株,占筛选总菌株数量的5.23%,其中7株产酶活性较强;复筛结果显示菌株6hRe76产木聚糖酶活力最高,其发酵液木聚糖酶酶活为57.15U/mL,初步鉴定该菌株为克里布所类芽胞杆菌(Paenibacillus kribbensis).该研究为木聚糖酶的生产提供了潜在的新资源.     

兰——3酵母菌的鉴定

遵照伟大领袖毛主席“从矿物中提取食品”的教导,甘肃省科技局组织了石油酵母饲料化研究的会战。开展工作以来,以兰—3酵母菌为试验菌株。(兰—3酵母菌是兰州炼油厂研究所为进行微生物深度脱蜡试验而筛选出来的一个菌株,以下简称兰—3)为了确定该菌的分类地位和某些生物学特性,我们对该菌进行了鉴定。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with