Oil, Fatty Acid Profile and Karanjin Content in Developing Pongamia pinnata (L.) Pierre Seeds

Oil content, fatty acid composition and karanjin content were studied in developing pongamia seeds, at intervals of 3?weeks from 30?weeks after flowering up to 42?weeks. Three marked stages in seed development were observed at the early green pod stage, the middle half brown stage and the late dark brown stage. Significant variation in seed biomass, pod and seed characteristics were observed. A significant (P?<?0.01) decrease in the moisture content of the seeds was observed during seed development. The oil content gradually increased from 32.06 to 36.53?% as the seed matured. A significant variation in fatty acid composition was detected across all stages of seed development. Palmitic acid (16:0) content marginally decreased from 11.81 to 10.18?%, while stearic acid (18:0) and linolenic acid (18:3) remained constant at all stages of seed maturity. A steady increase in oleic acid (18:1) content from 38.11 to 49.11?% was observed, while the linoleic acid (18:2) content decreased from 30.14 to 18.85?%. The iodine value increased, while the saponification number of oil decreased during seed development. The increase in karanjin content was steady. Seeds harvested after 42?week after flowering yielded the maximum oil with high oleic acid content which could be suitable for biodiesel production.     

Fatty acid development in a soybean mutant with high stearic acid

The fatty acid composition of developing soybean (Glycine max [L.] Merrill) seeds was evaluated in the mutant line, A6, and its parent, FA8077. Seeds of both lines were harvested at 2-day intervals from 15 to 39 days after flowering (DAF) and at 4-day intervals from 39 DAF until maturity. Significant differences between the two lines were observed for stearic and oleic acid percentages at 19 DAF. The maximum difference between the lines was at 25 DAF, when A6 had 45.4% and FA8077 had 4.1% stearic acid. The increase in stearic acid percentage in A6 was accompanied by a decrease in oleic acid to 16.8% at 25 DAF, compared with 53.7% oleic acid for FA8077. The two lines did not differ in development of palmitic, linoleic and linolenic acids. The protein and oil content of mature seeds were similar for the two lines.     

Fatty Acid Composition of the Oil from Developing Seeds of Different Varieties of Safflower (Carthamus tinctorius L.)

Fatty acid composition and moisture and oil content were determined for Montola-2001 and Centennial safflower varieties at three different harvest dates from flowering to maturity, which were grown as autumn and spring crops in two different locations in 2001–2002 and 2002–2003. The experiment was carried out using split–split plots in a randomized complete block design with three replicates. Sowing dates affected oil content and fatty acid compositions significantly (P < 0.01), whereas moisture content in both years was not significantly affected. Moisture content declined 15 days from flowering period to maturity, while oil content increased. The rate of the palmitic acid formation decreased in both varieties 15 days from flowering period to maturity, whereas formation rates of the oleic and linoleic acids increased in Montola-2001 and Centennial varieties, respectively.     

Changes in Fatty Acids,Tocochromanols, Carotenoids and Chlorophylls Content During Flaxseed Development

A comparative study was performed to determine tocopherols, tocotrienols, fatty acids, and pigments content during the development of three varieties of flaxseed (H52, O116 and P129). Seed samples were collected at regular intervals from 7 to 56 days after flowering (DAF). The highest content of chlorophyll (89.72–130.5 mg/kg oil) was detected at 7 DAF. The maximum level of carotenoids (52.10–65.55 mg/kg oil) was reached at 21 DAF. During seed development, unsaturated fatty acids are the major component, reaching 85% of the total fatty acids while saturated fatty acids content were about 15%. The maximum level of γ-tocopherol (585 mg/kg oil) was reached at 42 DAF in P129 variety. These results may be useful for evaluating the flaxseed quality and determining its optimal harvest period.     

Comparative rates of formation of fatty acids in the soybean seed during its development

Summary Lincoln soybeans harvested at successive stages of maturity showed continuous increases in amounts present of each of the fatty acids: saturated, oleic, linoleic, and linolenic. The iodine value and linolenic acid percentage of the oil decreased somewhat during the early stages of seed development. The linoleic acid and total oil percentage in the bean increased continuously until the 50th day then remained constant. Oleic and saturated acids fluctuated. No evidence for dehydrogenation of saturated fatty acids was obtained either in the oil analyses or in tests of soybean tissues for dehydrogenase activity. Journal Paper No. 717 of the Purdue Agricultural Experiment Station, Lafayette, Ind.     

Temperature effects upon the expression of a high oleic acid trait in soybean

Soybeans (Glycine max L. Merr. cvs. N78-2245 and Dare) were grown to maturity under controlled environments to investigate temperature effects upon the fatty acid composition of developing seed. These genotypes exhibited genetic differences in oleic acid (18:1) content. Mature seed from N78-2245 germplasm normally contained ca. 43 mol% 18:1, and Dare seed contained ca. 18 mol% 18:1. When grown at 30/26 C or 22/18 C, the overall response of these genotypes to temperature resulted in lower 18:1 and higher linoleic (18:2) and linolenic (18:3) acid concentrations in mature seed. However, the genotypic response was much more pronounced in N78-2245 seed than in Dare seed. The basis for these genotypic differences appeared to be related to temperature effects upon the differentiation of the 18:1-synthetic and 18:1-desaturation mechanisms during seed development. Although the high-18:1 trait was expressed during N78-2245 seed development at both temperatures, high-18:1 glycerolipids accumulated during a shorter developmental period at 22/18 C than at 30/26 C. At 30/26 C, glycerolipids containing greater than 50% 18:1 were deposited between 20 and 45 days after flowering (DAF) and accounted for 84% (w/w) of the oil in mature seed. At 22/18 C, glycerolipids with similar fatty acid composition were formed between 30 and 45 DAF and accounted for only 40% (w/w) of the oil. Temperature effects upon 18:1-desaturation also appeared to mediate the overall differences in unsaturated fatty acid composition in these genotypes. The 18:1-desaturation mechanism in N78-2245 seed was more sensitive to temperature than that in Dare seed. These genotype-treatment combinations were ranked by degree of 18:1-desaturation in the order: Dare (22/18 C) = Dare (30/26 C) ≥ N78-2245 (22/18 C) > N78-2245 (30/26 C). It was proposed that the ranking of these genotype-treatment combinations may be attributed, in part, to the tissue levels of the 18:1-desaturase enzymes in soybean seed grown at different temperatures.     

Lipid changes in maturing oil-bearing plants. II. Changes in fatty acid composition of flax and safflower seed oils

Changes in the fatty acids composition of the oil in flax and safflower seed that occur during the seed-ripening period have been measured. Concentrations of lipid or of specific fatty acid, expressed on a weight-per-seed basis, have been plotted as functions of days after fertilization and of percentage of oil development. Relations between these two independent variables have been established, and limitations to the unsefulness of the latter variable have been pointed out. Days after fertilization proved to be the more useful abscissa. Nonpolar solvents were used to remove free lipid from the tissue, and the total extractable matter was separated into true lipid and nonlipid components. With both flax and safflower, weight of true free lipid per seed and total unsaturation increased during the same period of growth. Nonlipid extractable matter was an inverse function of the extent of development. In developing flax seed, oleic, linoleic, and linolenic acids all increased continuously; oil in immature seed however was more saturated than oil in more mature seed. Nevertheless the ratio of linolenic acid to linoleic acid that characterizes linseed oil was established by the 20th day after fertilization during a normal growing season. In developing safflower seed, oleic acid concentration increased slowly during the first 30 days after fertilization and then appeared to level off in some cases as maturity was approached. Initially linoleic acid was present in almost the same amount as oleic acid, but by the 20th day after fertilization its concentration was three times that of oleic acid. This ratio of linoleic to oleic acid tended to increase steadily during the latter part of seed development.     

番荔枝籽油脂脂肪酸组成的GC-MS分析

研究了番荔枝籽油脂中脂肪酸的组成.用索氏脂肪抽提器提取番荔枝籽的油脂,并以GC-MS分析油脂脂肪酸的组成.结果表明,番荔枝籽油脂收率达29.2％;番荔枝籽油脂中含有8种脂肪酸,主要为:油酸(45.37％)、亚油酸(30.68％)、棕榈酸(13.60％)和硬脂酸(8.94％),其中不饱和脂肪酸含量达76.29％.番荔枝籽含油量高,脂肪酸种类丰富,尤其是不饱和脂肪酸含量较高,具有较高的开发利用价值.     

Analysis and fatty acid composition of tobacco seed oils

Summary THE fatty acid compositions of twelve samples of oil representing a number of different types and varieties of tobacco were determined by the thiocyanometric method. The samples were remarkably uniform in composition, containing on the average 75% linoleic, 15% oleic, and 10% saturated acids. Spectrophotometric determination of the linoleic acid content of two samples of oil gave values 3.0 and 5.4% higher than those by the thiocyanometric method. A more complete investigation of the fatty acid constituents of one sample of flue-cured tobacco seed oil was carried out by analysis of fractions obtained by distillation of the methyl esters and by low-temperature crystallization of the distilled ester fractions. The composition calculated from these analyses agreed well with that determined from analysis directly on the oil. The saturated acids consisted of palmitic and stearic acids, the proportions being about 7 and 3%, respectively, of the total fatty acids. Analysis of this sample of oil showed that it contained 0.043% of tocopherol. From its composition, tobacco seed oil would seem to be particularly suitable for the manufacture of nonyellowing alkyds or for the preparation of technical linoleic acid. One of the laboratories of the Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, United States Department of Agriculture.     

番荔枝籽油脂脂肪酸组成的GC—MS分析

研究了番荔枝籽油脂中脂肪酸的组成。用索氏脂肪抽提器提取番荔枝籽的油脂,并以GC-MS分析油脂脂肪酸的组成。结果表明,番荔枝籽油脂收率达29．2％;番荔枝籽油脂中含有8种脂肪酸,主要为：油酸（45．37％）、亚油酸（30．68％）、棕榈酸（13．60％）和硬脂酸（8．94％）,其中不饱和脂肪酸含量达76．29％。番荔枝籽含油量高,脂肪酸种类丰富,尤其是不饱和脂肪酸含量较高,具有较高的开发利用价值。．     

Changes in the Content of Phenolic Compounds in Flaxseed Oil During Development

The phenolic fraction of flaxseed oil was quantified during the development of three varieties (H52, O116 and P129). Seed samples were collected at regular intervals from 7 to 56 days after flowering (DAF). During oilseed processing, less polar compounds are co-extracted with oil. The methanolic extracts were obtained by solid phase extraction. Separation of phenolic compounds was conducted by high-performance liquid chromatography-mass spectrometry. The main phenolic compounds detected during maturation were: diphyllin, pinoresinol, matairesinol, secoisolariciresinol, vanillic acid, ferulic acid and vanillin. The highest amount of lignans (6.74 mg of analyte/kg of flaxseed oil) was detected at 7 DAF in P129 variety. The maximum level of phenolic acids (2.57 mg of analyte/kg of flaxseed oil) was reached at 7 DAF in P129 which had also the highest content of simple phenols (1.37 mg of analyte/kg of flaxseed oil) at the same date after flowering. At full maturity, the content of phenolic compounds in three varieties ranged from 0.26 to 0.36 mg of analyte/kg of flaxseed oil. The highest content of total phenolic compounds using the Folin–Ciocalteu method was detected in P129 variety (196.42 mg CAEs/kg of flaxseed oil) at 7 DAF. Results of this study indicate that flaxseed oils contain different amounts of phenolic compounds using different methods.     

Association of seed size with genotypic variation in the chemical constituents of soybeans

Ten soybean genotypes grown in 1992 with seed size ranging from 7.6 to 30.3 g/100 seeds and maturity group V or VI were selected and tested for oil and protein content and for fatty acid composition. In these germplasm, protein varied from 39.5 to 50.2%, oil, 16.3 to 21.6%, and protein plus oil, 59.7 to 67.5%. Percentages of individual fatty acids relative to total fatty acids varied as follows: palmitic, 11.0 to 12.8; stearic, 3.2 to 4.7; oleic, 17.6 to 24.2; linoleic, 51.1 to 56.3 and linolenic, 6.9 to 10.0. Seed size showed no significant correlations with individual saturated fatty acids, protein or oil content. However, significant correlations were found between seed size and individual unsaturated fatty acids: positive with oleic, and negative with linoleic and linolenic. Oil and protein content were negatively correlated with each other. Among the major fatty acids, only the unsaturated were significantly correlated with each other: negative between oleic and linoleic or linolenic, and positive between linoleic and linolenic. A subsequent study with soybeans grown in 1993 generally confirmed these findings. Variation in relative percentages of unsaturated fatty acids andr values for most pairs of relationships were even higher than those obtained from the 1992 crop. Presented at the 85th AOCS Annual Meeting and Expo, Atlanta, Georgia, May 8–12, 1994.     

越南安息香种子脂肪酸的在线甲基化-GC分析研究

建立了分析越南安息香种子油、果实和果壳的脂肪酸组成的在线甲基化-气相色谱法。将微克级的安息香样品与2μL衍生化试剂三甲基氢氧化硫（0.2 mol/L）加入裂解器,在350℃下瞬间反应,由气相色谱在线检测到8种脂肪酸甲酯成分,主要有棕榈酸（ C16∶0）、硬脂酸（ C18∶0）、油酸（ C18∶1）、亚油酸（ C18∶2）和亚麻酸（ C18∶3）,不饱和脂肪酸含量在84.5%以上,其中亚油酸含量最高,达47.29%。5次平行测定的相对标准偏差（ RSD）小于3.81%。并结合相似性分析法比较了4种不同产地的安息香种仁与6种食用油的脂肪酸组成,相似性结果表明不同产地的安息香种仁的脂肪酸组成相似,其脂肪酸组成与食用植物油相近,与玉米油的组成分布最为接近,相似系数在0.987~0.990,且越南安息香种子中人体必需的多不饱和脂肪酸含量（ C18∶2和C18∶3）与大豆油和葵花籽油相近,高于一般植物油,具有较高的营养价值。结果表明该法简便、快速、准确,适合越南安息香种子油脂的测定。     

Soybean seed composition under high day and night growth temperatures

High diurnal temperatures often affect development of soybean [Glycine max (L.) Merr.], but little is known about the relative influence of high day and night temperatures on the chemical composition of the seed. This study was conducted to determine the effects of combinations of high day and night temperatures during flowering and pod set (R1–R5), seed fill and maturation (R5–R8), and continuously during the reproductive period (R1–R8) on soybean seed oil, protein, and fatty acid composition. Day/night temperatures of 30/20, 30/30, 35/20, and 35/30°C were imposed on the soybean cultivar Gnome 85 in growth chambers. The day/night temperature combinations during R1–R5 had little effect on the oil and protein concentration and the fatty acid composition of seed produced. As mean daily temperature increased from 25 (30/20) to 33 (35/30)°C during R5–R8 and 25 (30/20) to 33 (35/30)°C during R1–R8, and oil concentration decreased and protein concentration increased. Increased day temperature during R5–R8 and R1–R8, averaged across the two night temperatures, increased oleic acid and decreased linoleic and linolenic acids. When night temperature was increased at 30°C day temperature during R5–R8 and R1–R8, oleic acid decreased and linoleic acid increased. When night temperature was increased at 35°C day temperature during R1–R8, oleic acid increased, and linoleic and linolenic acids decreased. These results indicate the importance of high day and night temperatures during seed fill and maturation in the oil, protein, and fatty acid composition of soybean seed.     

Seed oils fromCitrus sinensis

Comparative analyses of seed oils from the four most important orange varieties at different stages of maturity have shown remarkably similar fatty acid content by GLC. Percentage distribution of fatty acids, refractive index, and iodine number could probably be used to differentiate or help confirmCitrus species since there is enough variation between species to make a valid comparison. Seed content was noted as being related to fruit maturity, as was moisture content of seeds. The oil content of pineappleorange seeds was found to be variable, correlated to moisture content of seeds, and it reached a maximum when seed moisture had decreased to approximately 49%. Journal Series No. 1662. University of Florida Agricultural Experiment Stations.     

Impact of development periods on chemical properties and bioactive components of safflower (Carthamus tinctorius L.) varieties

This study aimed to determine the chemical properties (fatty acid composition, oil content, sterol and tocopherol compositions) of the oils extracted from the seeds of safflower (Dinçer, Remzibey, Balci, Linas, Yenice, Olas) varieties harvested in different periods from flowering to ripening period. In parallel with the increase of harvest time, the humidity rate decreased, while the oil ratios increased. It was determined that palmitic (16:0) and stearic (18:0) acids, which are significant saturated fatty acids, and oleic (18:1) and linoleic (18:2) acids, which are unsaturated fatty acids, are quite high in the oils of all safflower varieties. These fatty acids showed significant changes from the first harvest to the last harvest. The total saturated fatty acid ratios decreased, while the amount of unsaturated fatty acids increased as the maturation progressed. The first and latest harvest samples of Dinçer, Remzibey, Balcı, Linas, Yenice, Olas cultivars were selected and their sterol and tocopherol compositions were examined. The highest level of sterol in all cultivars was β-sitosterol and the amount of sterols decreased towards full maturity. It was determined that α-tocopherol was the dominant tocopherol found in the safflower oils and the amount of tocopherol increased towards full maturity.     

Studies on the changes in fatty acid composition in developing seeds. I.

Oils from castor seeds at different stages of ripening have been studied. The fatty acid composition has been determined by paper chromatography. The ratio of the weight of the kernel to the weight of the seed coat changes from 1.0: 1.24 (14 days) to 1.0:0.48 (45 days) and the oil content of the seed coat is negligible. Amounts of the individual fatty acids in 1 g of kernel as well as in a single seed have been shown. The amounts of ricinoleic, linoleic and stearic acids gradually increase with the ripening of the seeds whereas the amounts of oleic and palmitic acid after an initial increase upto 28 days gradually decrease towards the later stages of growth when the amounts are calculated on the basis of a single seed.     

In vivo and in vitro lipid accumulation inBorago officinalis L.

Seeds of 13 accessions of borage (Borago officinalis) varied in total fatty acid content from 28.6 to 35.1% seed weight, with linoleic, γ-linolenic, oleic and palmitic as the predominant fatty acids, averaging 38.1%, 22.8%, 16.3% and 11.3% of total fatty acids, respectively. There was an inverse relation between γ-linolenic acid (25.0 to 17.6%) and oleic acid (14.5 to 21.3%). Fatty acid content of leaf tissues was 9.1% dry weight, with α-linolenic acid 55.2% and γ-linolenic acid 4.4% of total fatty acids. Cotyledons were the major source of fatty acids in seeds. Seed fatty acid content increased from <1 mg at six days postanthesis to about seven mg at maturity (22 to 24 days). Individual fatty acid content of seed was relatively constant after day 8. When immature embryos from 6 to 16 days postanthesis were cultured in a liquid or semisolid basal medium, fatty acid composition was similar to that of in vivo-grown seeds. Growth of cultured embryos decreased as sucrose concentration was increased from 3 to 20% in the basal medium, and most embryos did not survive 30% sucrose; fatty acid as a percentage of dry weight was maximal at 6% sucrose.     

Changes in the structure of soybean triglycerides during maturation

Soybeans of the Hawkeye variety were picked at eleven periods from 30 to 111 days after flowering and extracted with chloroform-methanol. The triglyceride fraction of five pickings, selected 35 to 91 days after flowering (when synthesis of lipid was most active), were isolated by silicic acid thin layer chromatography (TLC) and species composition determined using argentation TLC and lipase hydrolysis. The triglyceride content of the total lipid increased from 6.5% at 30 days after flowering to 85% in the mature bean (111 days). The major changes in fatty acid composition of the triglycerides occurred during the first 52 days after flowering. During this period linolenic acid decreased from 34.2% to 11.7%, the percentages of linoleic and oleic acids increased, stearic remained fairly constant and palmitic decreased slightly. Large quantitative changes occurred in the molecular species of the triglycerides of the bean during maturation; some triglycerides containing linolenic acid could not be detected approximately 66 days after flowering. Although changes occurred in the percentage and amount of each triglyceride species, the positional distribution of fatty acids remained virtually unchanged throughout maturation. Linolenic acid was distributed fairly uniformly between the β-position and the α-positions, linoleate favored esterification in the β-position, and oleate the α-positions. Most of the stearic and palmitic acids were esterified in the α-positions. The consistency of the positional arrangement of the fatty acids indicated that the mode of glyceride synthesis was established very early during maturation and molecular species composition was controlled by the fatty acids available for synthesis.     

Variation in composition of sunflower oil from composite samples and single seeds of varieties and inbred lines

The seed oil of eight sunflower varieties grown at 10 locations in 1964 and 14 locations in 1964 showed highly significant differences between varieties and between stations in mean values for percentage of stearic, oleic and linoleic acids but no significant difference for palmitic acid. The same observations held for oleic and linoleic acids in three varieties common to eight stations in the two years. The only significant interaction appearing in these studies was between years and stations. Varieties requiring the same time to mature differed significantly. Oil from composite samples of inbred lines showed large differences in composition, e.g., the ranges in 56 lines grown in one season at one location were: palmitic 4.7–8.2%; stearic 1.7–9.1%; oleic 13.9–40.3%; and linoleic 47.9–76.4%. Single seeds within inbred lines also showed striking variation. The greatest variation occurred in lines inbred for one to three generations and the least in lines inbred for eight to nine generations. Pairs of lines with identical or similar flowering date differed significantly in mean values of all four acids. Variation between seeds within varieties were relatively narrow in Armavirec and Advent, but wide in Peredovik where the range was: palmitic 4.5–9.4%; stearic 2.5–12.4%; oleic 14.8–46.4%; and linoleic 34.3–75.5%. The results show that genetic control of oil quality, independent of flowering or maturity date, exists in sunflowers. The wide range in composition suggests that altering oil quality in the crop by breeding is a practical objective. Contribution No. 73, Research Station, Research Branch, Canada Department of Agriculture, Morden, Manitoba and contribution No. 97, Analytical Chemistry Research Service, Ottawa. Presented at the AOCS Meeting, Chicago, October 1967.