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以桢楠(Phoebe zhennan)新鲜叶片、硅胶干燥叶片为材料,研究了DNA的提取方法,并对影响RAPD反应的各因素进行了优化。得到了桢楠RAPD的优化反应体系及程序,适合桢楠RAPD反应的体系总体积为25μL,DNA模板2μL,Taq DNA聚合酶用量0.3μL,引物用量1μL,Mg2+用量5μL,dNTPs用量0.5μL,ddH2O16.2μL;适合桢楠RAPD扩增的程序是94℃预变性3 min,94℃变性1min,36℃复性1 min,72℃延伸2 min,42个循环,最后72℃延伸10 min,产物于4℃保存,同时结果表明硅胶保存的样品完全可以与传统的新鲜叶片得到同样的PCR扩增结果,完全可以满足研究需要。 相似文献
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本文对采自全国8个省市的落叶松—杨栅锈茵茵株进行了基因组DNA提取,并以其为模板对影响RAPD扩增1的重要参数进行优化试验,以期建立落叶松—杨栅锈茵RAPD反应的优化体系。结果表明,PCR扩增体系最佳条件是:25μL体积中,2.5mmol/LM^2 ,30ng/μL模板,0.15mmol/LdNTP,1.5μL引物,0.75UTaq聚合酶。 相似文献
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以麻竹DNA为模板,对影响麻竹RAPD扩增的重要参数进行了优化试验,以期建立麻竹RAPD反应的最适体系。最终得出的麻竹RAPD反应体系为:20μL反应体系,2μL10倍反应缓冲液,模板含量为50ng,2 5mmol·L-1的Mg2+,1 5U的Taq酶,dNTP为1 75mmol·L-1,引物浓度为0 4μmol·L-1。优化后的RAPD反应程序为:94℃3min→[94℃1min→37 5℃1min→72℃1min20sec]40个循环→72℃8min→4℃保持。 相似文献
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采用改良CTAB法得到了可用于RAPD分析的羽衣甘蓝基因组DNA,摸索建立了适合羽衣甘蓝基因组的RAPD反应体系,运用优化的反应体系对羽衣甘蓝新品种的亲缘关系进行了聚类分析. 相似文献
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采用改良CTAB法得到了可用于RAPD分析的羽衣甘蓝基因组DNA,摸索建立了适合羽衣甘蓝基因组的RAPD反应体系,运用优化的反应体系对羽衣甘蓝新品种的亲缘关系进行了聚类分析。 相似文献
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我省板栗主栽品种(无性系)RAPD反应体系的建立 总被引:1,自引:0,他引:1
在RAPD反应中,有许多影响结果的稳定性和准确性.本项研究采用浙江省8个板栗主栽品种(无性系),对RAPD各种反应条件进行了探索,结果表明,板栗理想的反应体系为20μl反应体积含有100mmol/LTris-Hcl,500mmo1/LKCl,20mmol/LMgCL2,0.01%Gelatin,dATP、dTTP、dCTP、dGTP的量各为100μmol/L,50~200ng引物,0.75~1.0单位Taq酶,2~10ng模板DNA.反应条件为94℃预变性2min,再94℃30s、40℃30s、72℃1.5min扩增38个循环;最后在72℃延伸7min.应用上述反应体系进行板栗RAPD反应,扩增产物在1.0%琼脂糖凝胶中电泳,经EB染色后在紫外灯下观察照相,可获得满意的DNA指纹图谱. 相似文献
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橡胶树胶乳生理诊断法是一批法国学者于20世纪80年代发展起来的,用于判断胶树代谢与健康状况的方法。在研究了巴西橡胶树胶乳的各种成分在胶树中的生理功能及其变化规律,以及这些成分与胶树产量之间的相关性后,可以将无性系划分为不同的代谢类型,并根据不同的代谢类型,安排不同的采胶方法;同时也可以根据随机抽样理论找到这些成分的标准参考值,用来判断开割胶树的健康状况,以指导采胶生产。现在胶乳生理诊断主要用于无性系代谢类型判断与采胶强度判断。 相似文献
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S. Aleixo A. C. Gama-Rodrigues M. G. Costa M. V. S. Sales E. F. Gama-Rodrigues J. R. B. Marques 《Agroforestry Systems》2017,91(3):423-437
Little is known about the phosphorus (P) fractions and P lability of agroforest soils in tropical regions, particularly those of cacao (Theobroma cacao L.) agroforests. We hypothesized that the effect of P fertilization on the distribution of P fractions in the soil based on the source-sink relationship differs for different cacao agroforestry systems. The cacao agroforestry systems studied were the following: open cacao-cabruca, closed cacao-cabruca, cacao + erythrina, mixed cacao + rubber tree, and cacao + rubber tree intercropping. A natural forest and an unfertilized pasture were used as reference systems. The P fractions were determined using the Hedley sequential extraction method, and the P transformation processes were evaluated via structural equation modeling. The impact of low P fertilizer input on the P fractions varied according to the specific environmental conditions of each cacao production system. Consequently, there was high dissimilarity among all of the cacao sites. In all of the cacao agroforestry systems, there was an increase in inorganic P (Pi), especially the labile fraction (resin-Pi and NaHCO3-Pi), but organic P (Po) increased only in the cacao + erythrina system and in the rubber tree planting row of the cacao + rubber tree intercropping system. As a result, the fitted structural models indicated that the inter-relationships of the geochemical processes were more important for determining the P availability than the biological processes. However, the Po concentrations and relative proportion were high in all of the cacao agroforestry systems, thereby revealing the high potential of supplying P to plants via the mineralization process in the eventual removal of mineral fertilization. 相似文献
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橡胶树风灾保险处理实践与改进 总被引:1,自引:0,他引:1
简要描述了热带风暴"天鹅"对海南农垦橡胶树造成的灾害,分析了风灾保险的处理过程和发现的问题,探讨保险条款释义和实务操作等环节上的改进,以期完善海南橡胶树风灾保险工作。 相似文献
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国内外橡胶树种质资源收集保存及其研究进展 总被引:3,自引:0,他引:3
对世界各主要植胶国橡胶树种质资源的收集与保存、鉴定评价与利用、共享与保护现状,和分子标记技术在橡胶树种质资源鉴定评价中的运用作了综述;同时根据我国橡胶树种质资源研究与国外的差距和存在的主要问题,提出关于提高我国橡胶树种质资源保存和研究水平的建议及近期的主要研究内容。 相似文献
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云南橡胶树小蠹虫研究 总被引:2,自引:1,他引:1
云南植胶区橡胶树小蠹虫为4个科17个种的小蠹虫群体,是主要蛀食橡胶树死亡组织的次期性害虫,长期生存在橡胶林中。1990年后,橡胶树小蠹虫为害逐渐明显,表现大量发生,虫害树快速死亡,造成严重的经济损失。经2000~2007年的研究表明:橡胶树小蠹虫种群结构已发生重要变化,活蛀性的角面长小蠹(Platypussecrelus Sampson)、小杯长小蠹(P.calicutus Chapuis)已发展成为优势种,致使橡胶树小蠹虫种群从次要害虫逐渐变为主要害虫,成为橡胶树衰退重要的促进因素(Contributing factors);橡胶树衰退,特别是过度的乙烯利刺激割胶导致的橡胶树衰退,是促变小蠹虫群体结构并逐渐发展成为主要害虫的主要诱因。因此,提高管理水平,调控生产技术,使胶林生态系统达到新的平衡,才是有效地控制橡胶树衰退趋势和控制小蠹虫为害于经济阈值之下的正确途径。 相似文献
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Wild service tree [Sorbus torminalis (L.) Crantz] is a forest tree widespread in Europe, characterized by a scattered distribution. Its hermaphroditic flowers
are pollinated by insects, and outcrossing is the prominent mating system, also due to the presence of gametophytic self-incompatibility.
Genetic diversity and differentiation of 22 populations from northwestern Italy were investigated by means of variation scored
at 53 polymorphic RAPD (Random Amplified Polymorphic DNA) loci. Populations differed in genetic variation, with Shannon diversity
index ranging from 0.166 to 0.469. According to Analysis of Molecular Variance (AMOVA), most of the genetic variation was
found within populations (61.78%) with a significant proportion of variance attributable to genetic differences between regions
(23.60%) and between populations (14.62%). Evidence for isolation by distance was found in the set of populations sampled.
The effect of habitat fragmentation on genetic variation was also evaluated. The efficacy of RAPD markers in analysing genetic
variation, and the contribution of the results in terms of the preservation of biodiversity are discussed. 相似文献