Effectiveness of gelatine and chitosan spray coating for extending shelf life of vacuum-packaged beef

The effects of applying gelatine (10%) and chitosan (1%) as spray coatings to extend the shelf life of vacuum-packaged beef was studied and compared against uncoated vacuum-packaged beef for up to 21 days. The impact of edible coatings on the water-holding properties of vacuum-packaged beef was investigated by LF-NMR together with physicochemical, microbiological and sensorial assessments. Chitosan coating significantly reduced lipid oxidation by ~30% to 36% and inhibited the growth of lactic acid bacteriaby ~ 1–2 log cfu compared to purge collected from uncoated and gelatine coated samples. It did not negatively affect the meat pH, tenderness and colour, and exhibited significantly higher sensorial acceptance compared to beef coated with gelatine. Both coatings were not effective in reducing purge loss in vacuum-packaged meat as the storage time increased. This study suggests that chitosan spray coating could be easily adapted to industrial plant settings as an antioxidant and antimicrobial application before carrying out meat vacuum packaging operation.     

散沫花提取物复合明胶膜对冷链运输及放置过程中牛肉品质的影响

本研究调查了以墨鱼皮明胶和散沫花提取物（HAE）作为天然保鲜剂制备出的功能性涂膜对模仿冷链运输及零售1、3、6和8 d牛肉贮藏损失、颜色、菌落总数、脂质氧化以及高铁血红蛋白、血红素铁和游离氨基酸含量的影响,综合评价了散沫花提取物复合明胶膜对牛肉冷藏保鲜效果的的影响。结果表明,与未涂膜样品相比,涂膜处理有效防止了水分的流失和高铁肌红蛋白的形成,保持了肉样的持水性和颜色特性（P<0.05）。随着放置期的延长,HAE+明胶膜通过在贮藏期减少脂质氧化程度、菌落总数和嗜冷菌数,显著延长了肉的货架期（P<0.05）。此外,根据游离氨基酸含量,涂膜处理可以显著降低蛋白水解过程的速率,而未涂膜处理的样品水解速率更快（P<0.05）。主成分分析表明,样品颜色的变化与pH、血红素铁含量、脂质氧化以及微生物繁殖存在高度相关性,而HAE+明胶涂膜良好地抑制了这些因素的发展。因此,HAE+明胶涂膜可以作为有效的活性保鲜方式,用于肉类的冷藏保鲜。     

Effect of Nanoemulsified and Microencapsulated Mexican Oregano (Lippia graveolens Kunth) Essential Oil Coatings on Quality of Fresh Pork Meat

Fresh meat is a highly perishable food. This work aimed to evaluate the influence of Mexican oregano (Lippia graveolens Kunth) incorporated into active coatings (ACs) spread on fresh pork meat as free (FEO), nanoemulsified (NEO), and microencapsulated (MEO) essential oil (EO), on its microbiological, physicochemical and sensory properties during 15 d at 4 ± 1 °C. Thymol and γ‐terpinene were identified in the EO. In vitro effect of 2.85 mg EO/cm² was tested against Brochothrix thermosphacta, Micrococcus luteus, Lactobacillus plantarum, Pseudomonas fragi, and Salmonella Infantis. FEO antioxidant capacity (DPPH assay) was significantly higher than that of thymol, NEO and MEO (93.53%, 89.92%, 77.79%, and 78.50% inhibition, respectively), and similar to BHA (96.03%) and gallic acid (95.57%). FEO, NEO, and MEO ACs on meat caused growth inhibition of lactic acid bacteria (5 log population reduction) and Pseudomonas spp. (4 log reduction), whereas ≤1.5 log population reduction was observed for B. thermosphacta and Salmonella Infantis. Meat microbiota was more efficiently controlled by MEO than by FEO or NEO. ACs delayed lipid and oxymyoglobin oxidation of fresh pork meat. After 15 d of cold storage meat added with EO coatings was desirable for panelists, whereas untreated (UT) samples were undesirable. Active coatings are a significant alternative method for fresh meat preservation.     

Impact of Added Encapsulated Phosphate Level on Lipid Oxidation Inhibition during the Storage of Cooked Ground Meat

The effect of levels (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) of added encapsulated (e) phosphate (sodium tripolyphosphate, STP; sodium hexametaphosphate, HMP; sodium pyrophosphate, SPP) on lipid oxidation inhibition during storage (0, 1, and 7 d) of ground meat (chicken, beef) was evaluated. The use of eSTP and eSPP resulted in lower and higher cooking loss (CL) compared to eHMP, respectively (P < 0.05). Increasing encapsulated phosphate level (PL) enhanced the impact of phosphates on CL in both chicken and beef samples (P < 0.05). Encapsulated STP increased pH, whereas eSPP decreased pH (P < 0.05). pH was not affected by PL. The highest orthophosphate (OP) was obtained with eSTP, followed by eSPP and eHMP (P < 0.05). The level of OP determined in both chicken and beef samples increased (P < 0.05) during storage. Increasing PL caused an increase in OP (P < 0.05). The highest reduction rate in the formation of thiobarbituric acid reactive substances (TBARS) and LPO for both meat species were obtained with eSPP, followed by eSTP and eHMP (P < 0.05). Increasing PL resulted in lower TBARS and LPO (P < 0.05). Findings suggest that encapsulated phosphates can be a strategy to inhibit lipid oxidation for the meat industry and the efficiency of encapsulated phosphates on lipid oxidation inhibition can be enhanced by increasing PL.     

Effects of Different End‐Point Cooking Temperatures on the Efficiency of Encapsulated Phosphates on Lipid Oxidation Inhibition in Ground Meat

Effects of 0.5% encapsulated (e) phosphates (sodium tripolyphosphate, STP; sodium hexametaphosphate, HMP; sodium pyrophosphate, SPP) on lipid oxidation during storage (0, 1, and 7 d) of ground meat (chicken, beef) after being cooked to 3 end‐point cooking temperatures (EPCT; 71, 74, and 77 °C) were evaluated. The use of STP or eSTP resulted in lower (P < 0.05) cooking loss (CL) compared to encapsulated or unencapsulated forms of HMP and SPP. Increasing EPCT led to a significant increase in CL (P < 0.05). Both STP and eSTP increased pH, whereas SPP and eSPP decreased pH (P < 0.05). The higher orthophosphate (OP) was obtained with STP or SPP compared to their encapsulated counterparts (P < 0.05). The lowest OP was determined in samples with HMP or eHMP (P < 0.05). A 77 °C EPCT resulted in lower OP in chicken compared to 74 and 71 °C (P < 0.05), dissimilar to beef, where EPCT did not affect OP. In encapsulated or unencapsulated form, using STP and SPP enhanced reduction in TBARS and lipid hydroperoxides (LPO) compared with HMP (P < 0.05). Regardless of the phosphate type, more effective lipid oxidation inhibition was achieved by the use of encapsulated forms (P < 0.05). Increasing EPCT resulted in lower TBARS in beef and higher LPO values in both beef and chicken samples (P < 0.05). Findings suggest that encapsulated phosphates can be a strategy to inhibit lipid oxidation for meat industry and the efficiency of encapsulated phosphates on lipid oxidation inhibition can be enhanced by lowering EPCT.     

Natural antioxidant extract from galangal (Alpinia galanga) for minced beef

Raw and cooked minced beef samples containing commercial antioxidants or galangal (Alpinia galanga) extract were evaluated for storage stability at 4 ± 1 °C. Thiobarbituric acid (TBA) values of raw and cooked samples containing galangal extract at 5 and 10% (w/w) were significantly (p < 0.05) lower than those of the controls. In raw beef, galangal extract at 10% (w/w) was as effective as α‐tocopherol at 0.10% (w/w) and butylated hydroxytoluene (BHT) at 0.02% (w/w) in inhibiting/minimising lipid oxidation. Galangal extract also delayed the induction period of lipid oxidation in cooked beef but was again observed to be effective at a higher concentration than the commercial antioxidants studied. No difference in total plate count was observed in raw beef, but samples with galangal extract had higher Hunter a (redness) and chroma (colourfulness relative to the brightness of the surroundings) values. However, cooked beef treated with galangal extract or antioxidants had lower Hunter a values and total microbial counts than the control. Thus galangal extract may be a possible natural antioxidant source for meat and meat products. © 2000 Society of Chemical Industry     

Action of plasma-activated lactic acid on the inactivation of inoculated Salmonella Enteritidis and quality of beef

This study aimed to investigate the effects of plasma-activated lactic acid (PALA) on Salmonella Enteritidis and beef quality. To improve the antibacterial efficiency of plasma-activated water (PAW), plasma-activated lactic acid (PALA) was prepared by treating lactic acid (0.05–0.20%) with a plasma jet for 40 to 100 s with subsequent application to the surfaces of beef slices (50 × 50 mm, approximately 10 g). Slices were analyzed for changes in surface color, pH, lipid oxidation and odor. In addition, secondary structure (β-sheet and random coil) of the beef protein was determined using Fourier transform infrared spectrometer. We showed that the antibacterial activity of PALA was closely related to the reaction of NO₂⁻ with H₂O₂ as well as the decomposition of NO₂⁻ under acidic conditions. UV-treated fresh beef slices were spot-inoculated with Salmonella Enteritidis (5.67 log CFU/g) and then immersed in PALA solutions for 20 s; leading to a reduction ranging from 1.24 to 3.52 log CFU/g. Data indicated that there was no negative effect of PALA on beef quality parameters. This study not only provided a theoretical basis for developing a novel decontaminant but also enabled PALA to find a way for practical application.Industrial relevanceIn China, a high concentration of lactic acid (2.0–2.5%) is a widely used bactericidal agent in the beef industry. However, it has a negative effect on the odor and protein of the product. The meat industry faces challenges in achieving the balance between the antimicrobial effect on meat and the quality of the product. Based on this, plasma-activated water (PAW) is a hopeful alternative decontaminant for microbial inhibition on meat, made by treating water with non-thermal plasma, containing reactive species, ions and charged particles, which is safe and environmentally friendly. This study demonstrated that plasma-activated lactic acid (PALA) was more effective and stable than PAW. The PALA treatment of beef slices not only led to the reduction of Salmonella Enteritidis but maintained beef quality. PALA appears a promising decontaminant for the meat industry.     

PET trays coated with Citrus extract exhibit antioxidant activity with cooked turkey meat

A natural Citrus extract with potential antioxidant activity was evaluated as an ingredient for the production of active food packaging. The extract, in methanol, was sprayed onto the surface of polyethylene terephthalate (PET) trays. For comparison, a second set of trays were prepared using α-tocopherol as the coating. The effectiveness of the two types of packaging in delaying lipid oxidation in cooked turkey meat slices, stored at 4 °C over 4 days, was compared using 2-thiobarbituric acid-reactive substances (TBARS) and hexanal assays. TBARS and hexanal values, for meat stored on the Citrus extract coated trays, were significantly lower (P < 0.01) than those of meat stored on uncoated (control) trays while α-tocopherol coated trays exhibited no significant effect compared to control trays (P > 0.05). The effectiveness of the Citrus extract coating, compared to the α-tocopherol coating, was attributed to its higher surface roughness, demonstrated by optical profilometry, and the higher level of release (solubility) of the antioxidant in water.     

Effects of Ascorbic Acid and Antioxidants on the Color of Irradiated Ground Beef

Irradiation significantly decreased the redness of ground beef (P < 0.05), and the visible color of beef changed from a bright red to a green/brown, depending on the age of meat. Addition of ascorbic acid (0.1%, wt/ wt) in ground beef prior to irradiation prevented color changes in irradiated beef, and the effect of ascorbic acid became greater as the age of meat or storage time after irradiation increased. Ground beef with added ascorbic acid had significantly lower oxidation‐reduction potential than the control (P < 0.05), and the low oxidation‐reduction potential of meat helped maintain the heme pigments in reduced form. Sesamol +α‐tocopherol had no effect in stabilizing color of irradiated beef.     

Use of Glandless Cottonseed and Sunflower Seed Products in Batter/Breading for Fried Beef Patties

This study indicates that defatted glandless cottonseed flour (GCF) 01 sunflower seed protein isolate (SI) incorporated in the batter/breading system can impart their antioxidant effect to the meat of fried beef patties. Coarsely ground lean beef patties were coated consecutively with dry ingredient (wheat flour for control and 1:1 mixture of wheat flour-GCF or wheat flour-SI for experimental samples), batter made of 1 part of dry ingredient and 2 parts of water, and finally dry ingredient. Ground beef and dry ingredients each contained 1% salt. Use of GCF or SI in the coating mixes greatly reduced the thiobarbituric acid values of the meat when the coated patties were fried and stored at 4°C for 5 days.     

Effects of Sonication and Edible Coating Containing Rosemary and Tea Extracts on Reduction of Peanut Lipid Oxidative Rancidity

Rancidity due to the lipid oxidation process is a critical factor influencing the quality of roasted peanuts. Sonication in combination with edible coatings added with plant extracts may extend the oxidative stability of peanuts. Peanuts were roasted at 178°C for 15 min, subjected to sonication in hexane for 10 min, and then coated with carboxymethyl cellulose (CMC) solution mixed with rosemary, tea extracts, and α-tocopherol and stored at 35°C for 12 weeks. The oxidative stability of the samples was investigated by measuring the oxidative stability index. Reduction in oxidation of 66.1% and 10.4% was observed for samples roasted and coated with extracts of rosemary and tea, respectively, as compared to uncoated sample. However, the oxidative stability of samples roasted–sonicated and coated with extracts of rosemary and tea was improved by 100.7% and 28.1%, respectively, in relation to the control. Sonication beyond coating improved the oxidative stability of the samples mixed with rosemary, tea extracts, and α-tocopherol by 10.3%, 12.1%, 34.6%, and 17.7%, respectively. The L ^* , a ^* , and b ^* values indicated that the peanut coated with CMC mixed with rosemary, tea extracts, and tocopherol at different concentration levels did not have significant (P < 0.05) color change during the 12-week storage at 35°C.     

Effects of an innovative dipping treatment on the cold storage of minimally processed Annurca apples

The effect of trehalose as an edible coating on minimally processed Annurca apple slices was studied during cold storage. The edible coating was prepared by dipping the fruit in a solution containing trehalose at 0.8%, sucrose at 1.0% and sodium chloride at 0.1%. During storage at 6 °C the following parameters were monitored: weight loss, colour (hue angle (h°) and whitening index (WI)), firmness, malic and ascorbic acids, polyphenol content, microstructure by scanning electron microscopy (SEM) and microbial count. The results showed that such a coating reduced the browning phenomena; in fact the WI and h° values were significantly lower in coated samples than untreated ones. Moreover, decreases in weight loss and in the reduction of organic acids were observed in coated samples. Electron microscopy slides of the cut tissue showed how the coating worked.     

Potential of peanut skin phenolic extract as antioxidative and antibacterial agent in cooked and raw ground beef

This study investigated the potential of peanut skin extract (PSE) as inhibitor of lipid oxidation in cooked and raw ground beef (GB) and as antimicrobial agent in raw GB. Results show that addition of PSE to raw GB before cooking significantly inhibited the formation of peroxides and TBARS in cooked GB during the refrigerated storage. PSE at concentration ≥0.06% was as effective as BHA/BHT at 0.02% in inhibiting lipid oxidation. PSE also inhibited the oxidation of meat pigments thereby preserving the fresh redness of treated meat when used at 0.02–0.10%. Microplate assay showed complete inhibition of test bacteria (Bacillus subtilis, Salmonella typhimurium, Staphylococcus aureus, Streptococcus faecalis and Escherichia coli) in the presence of PSE at 0.4% or higher. However, the antimicrobial effect of PSE in GB was less potent. Hence, PSE can primarily serve the dual purposes of preserving the colour of raw GB and preventing lipid oxidation in cooked products.     

Oxidative Stability of Batter-Breaded Restructured Nuggets Processed from Prerigor Pork

Restructured pork slices made with either prerigor or postrigor meat, with or without 1.5% of defatted glandless cottonseed flour (GCF). were batter-breaded with or without GCF in predust and batter-mix: Prerigor products had less cooking losses than postrigor products; GCF in the meat or coating reduced cooking losses. Prefried prerigor products were more susceptible to lipid oxidation and warmed-over flavor development than prefried postrigor products. GCF in the meat or coating markedly reduced the oxidative deterioration in nuggets stored at 4°C or ?20°C regardless of the rigor state of meat used for restructuring. Little lipid oxidation occurred in vacuum-packaged raw nuggets stored at ?20°C.     

Effect of muscle,ageing time and modified atmosphere packaging conditions on the colour,oxidative and microbiological stability of packed beef

The objective of this study was to evaluate the influence of two different vacuum ageing times (7 and 14 days) and the impact of the modified atmosphere packaging (MAP) configuration (gas/product ratios: 0.5 and 1 and gas composition: 70% O₂ + 30% CO₂ and 40% O₂ + 30% CO₂ + 30% N₂) on the quality of fresh beef during subsequent storage at 4 °C. For this purpose, three separate experiments were performed. For each experiment, two different muscles (Longissimus dorsi and Biceps femoris) were sampled from four double‐muscled Belgian Blue beef carcasses. Next to colour, also the evolution in microbial load, pH, O₂ and CO₂ in the headspace and lipid oxidation at the meat surface were evaluated. A vacuum ageing for 14 days compared with 7 days resulted in a higher initial microbial load on the day of MAP packaging, which resulted finally in a significantly shorter shelf life. This ageing effect was less pronounced on the colour stability and lipid oxidation of the meat samples. No significant influence of the packaging configuration on any of the analysed parameters (colour, microbial load, pH and lipid oxidation at the meat surface) was observed.     

Evaluation of the antioxidant activities and nutritional properties of ten edible plant extracts and their application to fresh ground beef

In this study, we assessed the antioxidant efficacy and nutritional value of 10 leafy edible plants and evaluated their potential as natural antioxidants for meat preservation. We measured total phenolic content, 2,2-diphenyl-1-picryl-hydrazil (DPPH) radical scavenging activity, and vitamin C, chlorophyll, and carotenoid contents of 70% ethanol and water extracts of the edible plants. Based on these results, we investigated the effects of butterbur and broccoli extracts on lipid oxidation in ground beef patties. Plant extracts and butylated hydroxytoluene (BHT) were individually added to patties at both 0.1% and 0.5% (w/w) concentrations. Thiobarbituric acid reactive substance (TBARS) values and color parameters were tested periodically during 12 days of refrigerated storage. TBARS levels were significantly lower (p ≤ 0.05) in the samples containing plant extracts or BHT than the non-treated control. In addition, the beef patties formulated with the selected plant extracts showed significantly (p ≤ 0.05) better color stability than those without antioxidants. These results indicate that edible plant extracts are promising sources of natural antioxidants and can potentially be used as functional preservatives in meat products.     

Chitosan and Protein Coatings Affect Yield, Moisture Loss, and Lipid Oxidation of Pink Salmon (Oncorhynchus gorbuscha) Fillets During Frozen Storage

The effects of chitosan (CH1 = 1% and CH2 = 2% solution), egg albumin (EA), soy protein concentrate (SPC), pink salmon protein powder (PSP), and arrowtooth flounder protein powder (AFP) as edible coatings on quality of skinless pink salmon fillets were evaluated during 3 mo frozen storage. Coating with 2% chitosan (CH2) resulted in significantly higher yield than coating with PSP and AFP. The thaw yield of salmon fillets coated with CH2 was higher than those of the control and fillets coated with AFP. The noncoated, CH1‐, and CH2‐coated fillets had similar drip loss (0.4% to 1.2%), which was lower than those observed for PSP‐ and AFP‐coated fillets. All fillet samples had similar cook yield (84.2% to 88.8%). The fillet coated with CH1, CH2, SPC, and EA had significantly higher (P < 0.05) moisture content after thawing than the control noncoated fillets. Coating with CH1 and CH2 was effective in reducing about 50% relative moisture loss compared with the control noncoated fillets. Chitosan (CH1 and CH2) and SPC delayed lipid oxidation. There were no significant (P > 0.05) effects of coating on a*, b*, and whiteness values for cooked fillets after 3 mo frozen storage.     

Synchronised interrelationship between lipid/protein oxidation analysis and sensory attributes in refrigerated minced beef meat formulated with Punica granatum peel extract

Microbiological, chemical and sensory effects of pomegranate peel ethanol extract (EE) at 0.1, 0.5 and 1% (w w⁻¹) on refrigerated minced beef meat were undertaken. Chemical analyses revealed that the treated meat underwent significant decrease (P < 0.05) of primary and secondary lipid oxidation. By the end of storage, EE at 1% reduced the MetMb, carbonyl group accumulation and the disappearance of sulphydryl proteins by 56.68, 65.71 and 59.69% respectively, compared with control samples. EE at 1% showed also the highest scores for colour, appearance, odour and overall acceptability (OA) attributes using a 9-point hedonic scale. Chemometric approaches showed that lipid/protein oxidation analysis, sensory attributes and storage time allowed the samples discrimination. Oxidative stability parameters were equally used to predict OA by linear regression analysis. The reported data provide an approach to strengthen the interpretation of sensory quality and how they are affected by chemical changes in newly formulated meat products.     

Shelf life of semifried tuna slices coated with essential oil compounds after treatment with anodic electrolyzed NaCl solution

The main objective of this study was to evaluate the effect of two commonly used essential oils after treatment with anodic electrolyzed NaCl solution in order to extend the shelf life of coated semifried tuna slices. Samples of tuna slices were treated with 100-fold electrolyzed water and coated with an edible solution containing 1% essential oil (0.5% eugenol [E] plus 0.5% linalool [L]). The coated slices were fried at 170 degrees C for 1 min and then stored at 5 and 25 degrees C. Total volatile basic nitrogen of slices treated with electrolyzed water and 1% (E+L) decreased from 15.5 for the nontreated control slices to 9.7 immediately after treatment and remained at a low level (<30) until the end of the storage period (day 20). Treatment with 1% (E+L) significantly suppressed lipid oxidation in coated semifried tuna. Sensory evaluation and microbiological assays showed that treatment with electrolyzed water and 1% (E+L) extended the shelf life of coated semifried slices to 15 and 2 days compared with 5 and 1 days for control slices during storage at 5 and 25 degrees C, respectively.