Ets—1在颌骨骨肉瘤中的表达

目的：分析Ｅｔｓ－１（Ｅ２６ ｔｒａｎｓｆｏｒｍａｔｉｏｎ－ｓｐｅｃｉｆｉｃ）在颌骨骨肉瘤的表达及意义。方法：采用免疫组织化学ＡＢＣ法检测Ｅｔｓ－１蛋白在２０例颌骨骨肉瘤和８例软骨瘤中的表达。结果：５５％（１１／２０）的骨肉瘤中Ｅｔｓ－１呈阳性表达，阳性表达率在各病理学分型间无显著性差异（Ｐ〉０．０５），而转移组明显高于未转移组（Ｐ〈０．０５）；１２．５％（１／８）的骨软骨瘤呈Ｅｔｓ－１阳性，与骨     

颌骨骨内瘤中p^27的表达及意义

目的：分析细胞周期素依赖激酶抑制物（ＣＫＩ）ｐ＾２７蛋白在颌骨骨肉瘤的表达及意义。方法：采用免疫组织化学ＡＢＣ法检测ｐ＾２７蛋白在２０例颌骨骨肉瘤的８例骨软骨瘤中的表达。结果：２０％（４／２０）的骨肉瘤中ｐ＾２７呈阳性表达,８７．５％（７／８）的骨软骨瘤呈ｐ＾２７阳性,阳性表达率与骨肉瘤相比有显著性差异（ｐ〈０．０５）。结论：ｐ＾２７在颌骨骨肉瘤中低表达并可作为骨肉瘤发生和发展过程中的一个抑癌基因标志物。     

口腔粘膜下纤维性变组织中内皮素1的免疫组织化学和定量研究

目的：探讨ＥＴ－１在口腔粘膜下纤维性变（ＯＳＦ）发病机制中的作用。方法：采用免疫组化染色ＳＡＢＣ法和图像分析技术,对ＯＳＦ早、中、晚期各１０例、口腔扁平苔藓（ＯＬＰ）１０例以及正常人１０例的颊粘膜组织中ＥＴ－１表达进行定量分析。结果：①ＯＳＦ组织中ＥＴ－１免疫阳性物质超量表达,正常及ＯＬＰ组织中ＥＴ－１表达微弱;②ＯＳＦ早、中、晚期组织中ＥＴ－１含量显著高于正常（Ｐ〈０．０１）,且早、中期显著高于晚期（Ｐ〈０．０１）;③ＯＳＦ早、中期组织ＥＴ－１含量显著高于ＯＬＰ（Ｐ〈０．０１）,晚期间质ＥＴ－１含量显著高于ＯＬＰ（Ｐ〈０．０５）,上皮ＥＴ－１含量两者差异不显著（Ｐ〉０．０５）;④口腔粘膜中上皮和间质的ＥＴ－１含量呈显著正相关（Ｐ〈０．０５）。结论：ＥＴ－１在ＯＳＦ的表达具有特异性,且可能影响ＯＳＦ病变的发生发     

颊癌中肿瘤转移抑制基因nm23mRNA的表达 Ⅰ.Northern斑点杂交研究

通过Ｎｏｒｔｈｅｒｎ斑点杂交技术研究５２例颊癌及相关组织中肿瘤转移抑制基因ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ的表达。结果发现：颊癌组织中ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ表达比正常颊粘膜、白斑、癌旁粘膜均有不同程度增高。颊癌有转移组中ｎｍ２３－Ｈ１ｍＲＮＡ表达比无转移组明显降低，转移灶中ｎｍ２３－Ｈ１ｍＲＮＡ的表达更低（Ｐ＜０．０５）。１１例有转移颊癌患者中有９例（８１．８％）为ｎｍ２３－Ｈ１低表达；而１９例无转移颊癌，有１５例（７８．９％）为高表达（Ｐ＜０．０５）。ｎｍ２３－Ｈ２ｍＲＮＡ表达在有无转移组患者中无明显差异（Ｐ＞０．０５）。结果提示，在颊癌的转移过程中，ｎｍ２３－Ｈ１比ｎｍ２３－Ｈ２起着更重要的作用。ｎｍ２３－Ｈ１ｍＲＮＡ表达可作为预测有无颊癌淋巴结转移的一个有价值的指标。     

癌基因c－erbB－2、抗癌基因p53在涎腺良恶性肌上皮瘤中的表达及分析

应用免疫组化方法对４０例涎腺肌上皮瘤（ＭＥ）、肌上皮瘤细胞生长活跃（ＭＡＰ）、恶性肌上皮瘤（ＭＭＥ）进行了ｃ－ｅｒｂＢ－２、ｐ５３基因蛋白表达的研究。结果：ｃ－ｅｒｂＢ－２表达总的阳性率为４２．５％（１７／４０），其中ＭＥ为１６．７％（２／１２）．ＭＡＰ为３７．５％（３／８），ＭＭＥ为６０％（１２／２０），三者间有显著差异（Ｐ＜０．０５），ｐ５３表达总的阳性率为２５％（１０／４０），ＭＥ，ＭＡＰ，ＭＭＥ三者间无显著差异（Ｐ＞０．０５）．ｃ－ｅｒｂＢ－２与ｐ５３在该瘤中的表达无相关性，结果表明ｃ－ｅｒｂＢ－２和ｐ５３的过量表达可能参与了涎腺肌上皮瘤的发生和分化过程；特别是癌基因ｃ－ｅｒｂＢ－２的检测对鉴别该瘤的良、恶性及早期诊断有一定的意义。     

口腔颌面部肿瘤患者T淋巴细胞亚群及细胞因子检测的临床意义

本文对４０例口腔颌面部恶性肿瘤及２０例良性肿瘤患者同时进行外周血Ｔ淋巴细胞亚群、ｓＩＬ－２Ｒ及ＴＮＦ三项指标检测，结果表明，１．良性、恶性患者均有ＣＤ３值明显下降（Ｐ＜０．０５、ｐ＜０．０ｌ），ｃＤ４／ＣＤ８比值明显下降（ｐ＜０．００ｌ），ＣＤ８值明显上升（Ｐ＜０．００１），并且良性高于恶性患者（ｐ＜０．０５），ｃＤ４值恶性患者有下降趋势（ｐ＞０．０５）。２．恶性患者ｓＩＬ－２Ｒ水平明显高于正常人（ｐ＜０．０１），也高于良性患者（ｐ＜０。０５）。３．ＴＮＦ水平三组尚无明显差异（Ｐ＞０。０５）。４．三项检验指标与恶性肿瘤临床分期有一定相关趋势。提示：Ｔ淋巴细胞亚群、ｓＩＬ－２Ｒ检测对口腔颌面部恶性肿瘤诊断及预后判断具有一定参考价值，ＴＮＦ检测的临床意义尚须进一步研究。     

牙龈成纤维细胞和牙周韧带细胞前列腺素含量分析

作者对体外培养的人牙龈成纤维细胞（ＧＦ）和牙周韧带细胞（ＰＤＬＣ）采用免疫组化ＡＢＣ染色及显微分光光度法分析，测定ＧＦ和ＰＤＬＣ中前列腺素Ｅ２（ＰＧＥ２）、６－酮一前列腺素Ｆ１ａ．（６－Ｋ－ＰＧＦ１ａ）和血栓素Ｂ２（ＴＸＢ２）含量，以探讨其与牙周炎的关系，结果表明ＧＦ的ＰＧＥ２吸光度为０．２５±０．０３，６－Ｋ－ＰＧＦ１ａ的吸光度为０．２０±０．０３，与对照组相比均有非常显著性差异；ＰＤＬＣ的吸光度分别为ＰＧＥ２０．２１±０．０２、ＴＸＢ２０．１６±０．０３和６－Ｋ－ＰＧＦ１ａ０．１３±０．０２，与对照组相比均有非常显著性差异。表明ＧＦ和ＰＤＬＣ均可分泌一定量的ＰＧＥ２、ＴＸＢ２和６－Ｋ－ＰＧＦ１ａ，但分泌的量略有差异，提示ＧＦ和ＰＤＬＣ可通过分泌ＰＧ，影响牙龈炎症变化和牙周组织再生。     

nm23基因在涎腺腺样囊性癌中的表达及其与肺转移的关系

为探讨肿瘤转移抑制基因ｎｍ２３与涎腺腺样囊性癌（ａｄｅｎｏｉｄｃｙｓｔｉｃｃａｒｃｉｎｏｍａ，ＡＣＣ）的关系，应用ＬＳＡＢ免疫组化染色方法，研究ｎｍ２３表达产物二磷酸核苷激酶（ｎｕｃｌｅｏｓｉｄｅｄｉｐｈｏｓｐｈａｔｅｋｉ－ｎａｓｅ，ＮＤＰＫ）在ＡＣＣ中的表达。结果：ＮＤＰＫ／ｎｍ２３在ＡＣＣ中有较高表达，阳性率为６４．０％（１６／２５）；其中，有肺转移者阳性率为１２．５％（１／８），无肺转移者阳性率为８８．２％（１５／１７），差异有极显著性（Ｐ＜０．０１）；ＮＤＰＫ表达与临床分期有关，分期越高，阳性率越低，差异有显著性（Ｐ＜０．０５），与病理分型差异无显著性（Ｐ＞０．０５）。结论：ｎｍ２３基因在人ＡＣＣ肺转移过程中起到抑制转移的作用，可作为临床颈侧ＡＣＣ患者转移和预后的指标。     

Ets-1在颌骨骨肉瘤中的表达

目的 :分析Ets- 1(E2 6transformation -specific)在颌骨骨肉瘤的表达及意义。方法 :采用免疫组织化学ABC法检测Ets- 1蛋白在 2 0例颌骨骨肉瘤和 8例骨软骨瘤中的表达。结果 :5 5 % (11 2 0 )的骨肉瘤中Ets - 1呈阳性表达 ,阳性表达率在各病理学分型间无显著性差异 (P >0 .0 5 ) ,而转移组明显高于未转移组 (P <0 .0 5 ) ;12 .5 % (1 8)的骨软骨瘤呈Ets - 1阳性 ,与骨肉瘤相比有显著性差异 (P <0 .0 5 )。结论 :Ets - 1在颌骨骨肉瘤中过表达并与骨肉瘤的转移有关。     

颊癌中肿瘤转移抑制基因nm23mRNA的表达 Ⅱ.定量逆转录多聚酶链反应检测

采用定量逆转录多聚酶链反应（Ｑ－ＲＴ－ＰＣＲ）技术检测ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ在４７例组织标本中的表达情况，发现ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ在颊癌原发灶、癌旁粘膜、正常颊粘膜、颌下腺、白斑、正常淋巴结和有转移的淋巴结中均有不同程度的表达。ｎｍ２３－Ｈ１在肿瘤转移组颊癌原发灶中的表达低于无转移组（Ｐ＜０．０５），ｎｍ２３－Ｈ２在颊癌有、无转移中的表达无差异（Ｐ＞０．０５）；ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２表达与临床病理参数间均无关（Ｐ＞０．０５）。结果提示：ｎｍ２３－Ｈ１ｍＲＮＡ表达下降与颊癌转移关系密切，而ｎｍ２３－Ｈ２ｍＲＮＡ表达与颊癌转移无关；通过Ｑ－ＲＴ－ＰＣＲ检测ｎｍ２３－Ｈ１ｍＲＮＡ的表达水平可为临床预测颊癌的转移提供有价值的指标。Ｑ－ＲＴ－ＰＣＲ技术的主要优点在于通过少量组织提取的总ＲＮＡ即可检测ｎｍ２３ｍＲＮＡ的表达。     

颌骨骨肉瘤中p~(27)的表达及意义

目的 :分析细胞周期素依赖激酶抑制物 (CKI)p2 7蛋白在颌骨骨肉瘤的表达及意义。方法 :采用免疫组织化学ABC法检测p2 7蛋白在 2 0例颌骨骨肉瘤和 8例骨软骨瘤中的表达。结果 :2 0 % (4 2 0 )的骨肉瘤中p2 7呈阳性表达 ,87 5 % (7 8)的骨软骨瘤呈p2 7阳性 ,阳性表达率与骨肉瘤相比有显著性差异 (p <0 0 5 )。结论 :p2 7在颌骨骨肉瘤中低表达并可作为骨肉瘤发生和发展过程中的一个抑癌基因标志物     

Ets-1在口腔扁平苔藓中的表达研究

目的 :分析Ets 1(E2 6transformation specific)在口腔扁平苔藓 (OLP)的表达及意义。 方法 :采用免疫组化ABC法检测Ets 1蛋白在 2 0例口腔扁平苔藓和 8例正常口腔黏膜组织中的表达。结果 :70 % (14 /2 0 )的口腔扁平苔藓病例中Ets 1呈阳性表达 ,明显高于正常黏膜组织 ,两者比较有显著性差异 (P <0 .0 5 )。阳性表达率在溃疡型OLP与斑块型OLP间亦有显著性差异 (P <0 .0 5 ) ,并与病程相关 (P <0 .0 5 )。结论 :Ets 1在口腔扁平苔藓中过表达并与其发病有关。     

人成釉细胞瘤中pRb和E2F-1表达与端粒酶活性的关系

目的　研究 pRb、E2F 1和细胞周期素E(cyclinE)在人成釉细胞瘤 (ameloblastoma,AB)中的表达及其与端粒酶 (hTERT)的关系 ,探讨其临床生物学意义。方法　用原位杂交和免疫组化SP法分别检测了AB中 pRb、E2F 1、cyclinE及hTERTmRNA的表达。结果　pRb在AB细胞核中阳性率为2 0 4 % ( 11/ 5 4 )。E2F 1mRNA阳性率为 92 6 % ( 5 0 / 5 4 )。cyclinEmRNA阳性率为 6 6 7% ( 36 / 5 4 )。hTERTmRNA阳性率为 94 4 % ( 5 1/ 5 4 )。伴随AB的复发与恶变 ,hTERT、E2F 1、cyclinE的表达逐渐增高 ,pRb表达丢失。hTERT与E2F 1、cyclinEmRNA之间经Spearman相关分析 ,rs 均为 1 0 0 0 ,P =0 0 0 0 1,呈高度正相关。结论　pRb/E2F 1与AB细胞的增殖、去分化相关 ,端粒酶活性的释放激活与pRb的低表达及E2F 1的高表达可能相关 ,并在G1晚期受cyclinE调节有较高表达     

MDM_2及p53在口腔疣状癌中的表达研究

目的:研究癌基因蛋白(MDM2)过度表达及p53功能失活与口腔疣状癌发生的相关性。方法:利用免疫组 织化学SP法检测13例口腔疣状癌、10例口腔高分化鳞癌、10例口腔低分化鳞癌组织中的MDM2蛋白及p53蛋白 表达,并分析其相关性。结果:口腔疣状癌中的MDM2蛋白表达与口腔鳞癌间差异无显著性,而其p53蛋白表达 明显低于口腔鳞癌(P<0.01);虽然两者之间无明显相关性(P>0.05),但在13例MDM 蛋白表达阳性的口腔疣 状癌中,仅4例(30.8％)呈现p53阳性,69.2％的病例呈现p53阴性表达。结论:MDM2蛋白过度表达与p53失活 并存可能与口腔疣状癌的发生相关。     

Immunohistochemical detection of retinoblastoma protein and E2 promoter-binding factor-1 in ameloblastomas

BACKGROUND: To clarify the roles of cell cycle regulation in oncogenesis and cytodifferentiation of odontogenic tumors, expression of retinoblastoma protein (RB) and E2 promoter-binding factor-1 (E2F-1) was analyzed in ameloblastomas as well as in tooth germs. METHODS: Tissue specimens of 10 tooth germs, 40 benign ameloblastomas, and five malignant ameloblastomas were examined immunohistochemically with the use of antibodies against RB, E2F-1, and phosphorylated RB. Ki-67 antigen immunostaining was made as a marker of cell proliferation. RESULTS: Immunohistochemical reactivity for RB, E2F-1, phosphorylated RB, and Ki-67 was detected in the nuclei of odontogenic epithelial cells near the basement membrane in tooth germs and benign and malignant ameloblastomas. The number of cells positive for phosphorylated RB was nearly equal to or slightly less than the number of cells positive for RB or E2F-1. The number of Ki-67-positive cells was slightly more than the numbers of cell positive for RB, E2F-1, or phosphorylated RB. The levels of immunoreactivity for RB, E2F-1, phosphorylated RB, and Ki-67 were slightly higher in benign and malignant ameloblastomas than in tooth germs. Plexiform ameloblastomas showed significantly higher expression of RB than follicular ameloblastomas. Ki-67 immunoreactivity was significantly higher in ameloblastic carcinomas than in metastasizing ameloblastomas. CONCLUSION: Similar immunoreactivity for RB, E2F-1, phosphorylated RB, and Ki-67 in tooth germs and ameloblastomas indicated cellular expression of phosphorylated RB and active-free E2F-1 in both normal and neoplastic odontogenic tissues. Expression of RB, E2F-1, and phosphorylated RB was considered to be involved in cell proliferation and differentiation of odontogenic epithelium via control of the cell cycle.     

Osteosarcoma of the jaw. The Chaim Sheba Medical Center experience

OBJECTIVE: The purpose of this article is to present 14 cases of osteosarcoma of the jaw treated at our medical center from 1989 to 1998. These cases are discussed in the light of a comprehensive review of 774 cases reported in the English literature over the past 3 decades. Differences between osteosarcoma of the jaws and osteosarcoma of the long bones are examined. SUBJECTS AND METHODS: The patients ranged in age from 8 to 78 years, the mean age being 33 years. Each patient had a histopathologically established diagnosis of osteosarcoma of the jaw. Records were reviewed for epidemiologic data, treatment modalities, and survival. RESULTS: Of the 14 patients, 6 (42%) had tumors in the mandible and 8 (58%) had tumors in the maxilla. Of the mandibular tumors, 5 occurred in the body of the mandible; all maxillary tumors originated in the alveolar ridge and involved the maxillary sinus. The chief complaint was an intraoral or extraoral painless swelling. Histopathologic types included chondroblastic, osteoblastic, fibroblastic, and malignant fibrous histiocytoma-like. Pathologic grade was determined to be high (3 or 4) in 13 cases and low (1) in only 1 mandibular case. All patients underwent surgical resection and immediate reconstruction. Adjuvant therapy included postoperative radiation (5 patients), postoperative chemotherapy (2 patients), and preoperative chemotherapy and postoperative radiation (1 patient). CONCLUSIONS: The results of the present study support the literature indicating that osteosarcoma of the jaw differs from osteosarcoma of the long bones in its biological behavior even though they have the same histologic appearance. Because of differences in tumor characteristics, the introduction of chemotherapy did not dramatically alter the prognosis of osteosarcoma of the jaw. Early diagnosis and radical surgery are the keys to high survival rates.     

颌骨骨肉瘤3例及文献复习

目的探讨颌骨骨肉瘤的临床病理特征、诊断及鉴别诊断方法,并进行相关文献复习。 方法回顾性分析韶关市第一人民医院3例颌骨骨肉瘤临床资料,结合影像学特点,分析该病光学显微镜下的形态特征和免疫组化结果,并进行相关文献复习。 结果3例颌骨骨肉瘤,病例1发生于左上颌、病例2发生于左下颌,病例1、病例2病理诊断为颌骨普通型骨肉瘤;病例3发生于右下颌,诊断为颌骨低级别中心性骨肉瘤。颌骨骨肉瘤在临床上十分少见,与长骨骨肉瘤相比有其自身特点。 结论颌骨骨肉瘤在发病年龄,镜下特征均有所不同,临床上要注意与良性骨病相鉴别,需结合临床特征、影像学及病理镜下形态综合考虑。     

Expression of hormonal receptors in osteosarcomas of the jaw bones: Clinico-pathological analysis of 21 case

Background: Sexual hormones have an important role in many hormone-dependant tumors like breast and prostate carcinomas, and also a relationship has been found with bone metabolism and bone tumors. Some studies have demonstrated that the expression of hormonal receptors (HR) in osteosarcomas (OS) of long bones is associated with gender, histological grade, histological type, and possibly may be connection with pathogenesis and evolution. However, to our knowledge there are no studies of HR in osteosarcomas of craniofacial bones (OS-CF). Objectives: To assess the expression of hormonal receptors in OS-CF. Material and Methods: Twenty one cases of OS-CF were included in this study. Clinical outcome was obtained from clinical charts. Histological sections were reviewed, and immunohistochemistry studies for estrogen, progesterone and androgen receptors were performed. Results: A striking female predominance was found (2:1), with a median age of 35 years. The predominant type of OS was osteoblastic (52.4%), and histological grade was high in 86%. Follow-up was obtained in 13 cases and ranged from 6 to 118 months (median 29 months). There were 8 patients (61.5%) dead or alive with progressive disease in the last follow up. Negative expression of HR was found in 19/21 cases; one showed weak nuclear expression for estrogen receptor, and another for androgen receptor. Progesterone receptor was negative in all cases. Conclusions: OS-CF mostly affected females, most of them were of the osteoblastic type and of high grade. Hormonal expression was practically negative in osteosarcoma of craniofacial bones. Key words:Osteosarcoma, jaws, estrogen, progesterone, androgen receptors.     

Osteosarcoma of the jaws in children

Two cases of osteosarcoma of the jaws in children are reported. One patient was a 13-year-old girl whose first symptoms included nasal and maxillary sinus congestion, followed by epistaxis. She was found to have chondroblastic osteosarcoma extending through the left maxillary alveolar process and sinus. Following surgery and chemotherapy, the patient has been free of disease for 7 years. The second patient, an 8-year-old boy, was diagnosed with juxtacortical (parosteal) osteosarcoma of the mandible, which is a less aggressive variant of the neoplasm. It is believed that this is the youngest patient reported to date with juxtacortical osteosarcoma of the jaws. He was treated by block resection of the right side of the mandible and is free of disease 3(1/2) years later.     

CyclinD_1在多形性腺瘤和多形性腺癌中的表达

目的　研究cyclinD1 在正常腮腺、多形性腺瘤 (PA)和多形性腺瘤癌变 (CPA)中的表达特点、表达水平及其与临床指标的关系。方法　应用免疫组化S P法检测cyclinD1 在 1 0例正常腮腺、37例PA和 1 4例CPA标本中的表达 ,图像分析cyclinD1 的表达水平。统计方法 :χ2 检验、ANOVA检验和t检验。结果　在CPA中cyclinD1 的表达以 ～ 为主 ,阳性率 78 6 % ,PA组中多为 -和 +,cyclinD1 在正常腮腺中表达与PA、CPA组差别显著 (P <0 0 5) ,CPA中cyclinD1 的表达水平也显著高于PA组 (P <0 0 5)。结论　cyclinD1 的异常表达在CPA的发生中起到重要的作用。