Microcosm investigations of stormwater pond sediment toxicity to embryonic and larval amphibians: variation in sensitivity among species

Stormwater ponds have become common features of modern development and often represent significant amounts of open space in urbanized areas. Although stormwater ponds may provide habitat for wildlife, factors responsible for producing variation in wildlife use of ponds have received limited attention. To investigate the role of variation in species tolerances of pollutants in structuring pond-breeding amphibian assemblages, we exposed species tolerant (Bufo americanus) and not tolerant (Rana sylvatica) of urbanization to pond sediments in laboratory microcosms. Pond microcosms had elevated sediment metal levels and chloride water concentrations. Among R. sylvatica embryos, exposure to pond sediments resulted in 100% mortality. In contrast, B. americanus embryos and larvae experienced only sublethal effects (i.e., reduced size at metamorphosis) due to pond sediment exposure. Our results suggest variation in pollutant tolerance among early developmental stages of amphibians may act in concert with terrestrial habitat availability to structure amphibian assemblages associated with stormwater ponds.     

Polyaromatic hydrocarbon exposure: an ecological impact ambiguity

Polyaromatic hydrocarbons (PAHs) represent a fraction of petroleum hydrocarbons and are currently one of the foremost sources of generating energy in today’s contemporary society. However, evidence highlighted in this review show that PAH pollution, as a result of oil spills, hazardous PAH-contaminated working environments and technologies which do not efficiently utilise fuels, as well as natural sources of emissions (e.g. forest fires) may have significant health implications for all taxa. The extent of damage to organisms from PAH exposure is dependent on numerous factors including degree and type of PAH exposure, nature of the environment contaminated (i.e. terrestrial or aquatic), the ability of an organism to relocate to pristine environments, type and sensitivity of organism to specific hydrocarbon fractions and ability of the organism to metabolise different PAH fractions. The review highlights the fact that studies on the potential damage of PAHs should be carried out using mixtures of hydrocarbons as opposed to individual hydrocarbon fractions due to the scarcity of individual fractions being a sole contaminant. Furthermore, potential damage of PAH-contaminated sites should be assessed using an entire ecological impact outlook of the affected area.     

Wastewater treatment and public health in Nunavut: a microbial risk assessment framework for the Canadian Arctic

Wastewater management in Canadian Arctic communities is influenced by several geographical factors including climate, remoteness, population size, and local food-harvesting practices. Most communities use trucked collection services and basic treatment systems, which are capable of only low-level pathogen removal. These systems are typically reliant solely on natural environmental processes for treatment and make use of existing lagoons, wetlands, and bays. They are operated in a manner such that partially treated wastewater still containing potentially hazardous microorganisms is released into the terrestrial and aquatic environment at random times. Northern communities rely heavily on their local surroundings as a source of food, drinking water, and recreation, thus creating the possibility of human exposure to wastewater effluent. Human exposure to microbial hazards present in municipal wastewater can lead to acute gastrointestinal illness or more severe disease. Although estimating the actual disease burdens associated with wastewater exposures in Arctic communities is challenging, waterborne- and sanitation-related illness is believed to be comparatively higher than in other parts of Canada. This review offers a conceptual framework and evaluation of current knowledge to enable the first microbial risk assessment of exposure scenarios associated with food-harvesting and recreational activities in Arctic communities, where simplified wastewater systems are being operated.     

A review of models for estimating terrestrial ecological receptor exposure to chemical contaminants

In instances where empirical measurements are not practicable, ecological risk assessments may rely on site-specific exposure models for estimating uptake of chemical contaminants. This paper presents, based on a review of the literature, a compilation of relatively simple quantitative models that can be combined to produce site- and species-specific first-order estimates of uptake of chemicals from abiotic media. These models have proved useful in providing order-of-magnitude estimates for screening and sample program design purposes. This paper intended as both a practical guide for choosing models to estimate terrestrial wildlife exposures and as a step toward development of a more comprehensive and standard approach to exposure assessment in terrestrial ecological receptors.     

Bioconcentration of trans-chlordane by the midge, Chironomus decorus

The accumulation and transport of the organochlorine pesticide, trans-chlordane, by the midge, Chironomus decorus was examined in a whole life cycle laboratory exposure assay. Larvae were kept in dosed water exposures including food particles from egg stages through adult metamorphosis and were sampled for weight gain and chemical content over the course of the 50 day study. Accumulation of trans-chlordane was related to the amount of contaminant in the larval environment. Concentration of trans-chlordane (ng contaminant/g tissue) in larvae was not significantly different from second through fourth instar stages but depended upon the weight of the larvae. Differences in time to metamorphosis, weight gain and sex ratios occurred among all replicate exposures, including controls, suggesting nongenetic maternal differences, rather than environmental variation caused by chemical exposure. When the larvae metamorphosed, an average of 82.6% of the contaminant was retained in the adults while 11.4% was left behind in the shed exuviae. This study suggests that terrestrial predators feeding on emerging aquatic insects can be exposed to organic pesticides from aquatic environments.     

State of the Art Report on Mathematical Methods for Groundwater Pollution Source Identification

The reliable assessment of hazards or risks arising from groundwater contamination problems and the design of efficient and effective techniques to mitigate these problems require the capability to predict the behavior of chemical contaminants in flowing water. Most attempts at quantifying contaminant transport have relied on a solution of some form of a well-known governing equation referred to as advection-dispersion-reaction equation. To choose an appropriate remediation strategy, knowledge of the contaminant release source and time release history becomes pertinent. As additional contaminated sites are being detected, it is almost impossible to perform exhaustive drilling, testing, and chemical fingerprint analysis every time. Moreover, chemical fingerprinting and site records are not sufficient to allow a unique solution for the timing of source releases. The purpose of this paper is to present and review mathematical methods that have been developed during the past 15 years to identify the contaminant source location and recover the time release history.     

State of the Art Report on Mathematical Methods for Groundwater Pollution Source Identification

The reliable assessment of hazards or risks arising from groundwater contamination problems and the design of efficient and effective techniques to mitigate these problems require the capability to predict the behavior of chemical contaminants in flowing water. Most attempts at quantifying contaminant transport have relied on a solution of some form of a well-known governing equation referred to as advection-dispersion-reaction equation. To choose an appropriate remediation strategy, knowledge of the contaminant release source and time release history becomes pertinent. As additional contaminated sites are being detected, it is almost impossible to perform exhaustive drilling, testing, and chemical fingerprint analysis every time. Moreover, chemical fingerprinting and site records are not sufficient to allow a unique solution for the timing of source releases. The purpose of this paper is to present and review mathematical methods that have been developed during the past 15 years to identify the contaminant source location and recover the time release history.     

Variation in sensitivity to cadmium among genetically characterized laboratory strains of the midge Chironomus riparius

Estimating the extent of intraspecific variation in sensitivity to contaminant exposure is important in order to explain variation in the outcome of toxicity tests and to predict the effects of chemical stress on natural populations of plants and animals. However, only few studies provide evident data concerning intraspecific variation in life-history traits caused by a differential response to chemical stress. In this study, we compared the life-history response of six laboratory strains of the midge Chironomus riparius to cadmium exposure in a full life-cycle assay. In addition, the level of genetic variation in all strains was measured at five variable microsatellite loci. Several significant differences in life-history traits among the strains were observed in controls and cadmium treatments. The extent of variation among strains was largest at moderate cadmium concentration (0.42 mg Cd/kg dw). At increased Cd concentrations all strains showed similar levels of high mortality and reduced reproductive success. All strains exhibited considerable levels of genetic impoverishment compared to field populations. Strains with low genetic variation showed reduced fitness in the controls and were more susceptible to Cd exposure. For instance, no reproductive success in the lowest Cd treatment was observed for the strain with the lowest level of genetic diversity. In contrast, this Cd concentration had no negative effects on life-history traits of more variable strains. These results confirm recent findings, that inbreeding and reduced genetic variation influence the reaction of populations towards environmental stress. In addition, we show that the level of genetic variation and inbreeding directly influences the outcome of toxicity tests and contributes to the lack of reproducibility of test results among laboratories.     

Contamination of an arctic terrestrial food web with marine-derived persistent organic pollutants transported by breeding seabirds

At Cape Vera, Devon Island (Nunavut, Canada), a colony of northern fulmars (Fulmarus glacialis) concentrates and releases contaminants through their guano to the environment. We determined whether persistent organic pollutants (POPs) from seabirds were transferred to coastal food webs. Snow buntings (Plectrophenax nivalis) were the most contaminated species, with ∑PCB and ∑DDT (mean: 168, 106 ng/g ww) concentrations surpassing environmental guidelines for protecting wildlife. When examined collectively, PCB congeners and DDT in jewel lichen (Xanthoria elegans) were lower in samples taken farther from the seabird colony, and increased with increasing δ¹⁵N values. However, only concentrations of p’p-DDE:∑DDT and PCB-95 were significantly correlated inversely with distance from the seabird cliffs. Linkages between marine-derived POPs and their concentrations in terrestrial mammals were less clear. Our study provides novel contaminant data for these species and supports biovector transport as a source of organic contaminants to certain components of the terrestrial food web.     

Upscaling heterogeneity in aquifer reactivity via exposure-time concept: forward model

Reactive properties of aquifer solid phase materials play an important role in solute fate and transport in the natural subsurface on time scales ranging from years in contaminant remediation to millennia in dynamics of aqueous geochemistry. Quantitative tools for dealing with the impact of natural heterogeneity in solid phase reactivity on solute fate and transport are limited. Here we describe the use of a structural variable to keep track of solute flux exposure to reactive surfaces. With this approach, we develop a non-reactive tracer model that is useful for determining the signature of multi-scale reactive solid heterogeneity in terms of solute flux distributions at the field scale, given realizations of three-dimensional reactive site density fields. First, a governing Eulerian equation for the non-reactive tracer model is determined by an upscaling technique in which it is found that the exposure time of solution to reactive surface areas evolves via both a macroscopic velocity and a macroscopic dispersion in the artificial dimension of exposure time. Second, we focus on the Lagrangian approach in the context of a streamtube ensemble and demonstrate the use of the distribution of solute flux over the exposure time dimension in modeling two-dimensional transport of a solute undergoing simplified linear reversible reactions, in hypothetical conditions following prior laboratory experiments. The distribution of solute flux over exposure time in a given case is a signature of the impact of heterogeneous aquifer reactivity coupled with a particular physical heterogeneity, boundary conditions, and hydraulic gradient. Rigorous application of this approach in a simulation sense is limited here to linear kinetically controlled reactions.     

Variability of pesticide exposure in a stream mesocosm system: macrophyte-dominated vs. non-vegetated sections

For flowing water bodies no information is available about patterns of contaminant distribution in flowing water compared to macrophyte-dominated structures. The aim of the study was to examine temporal dynamic and spatial cross-channel variability of pulse exposure of the insecticide thiacloprid in outdoor stream mesocosms. Two distinct cross-channel sections have been considered: macrophyte-dominated littoral and non-vegetated midstream. Median disappearance time ranged from 17 to 43 h (water phase, midstream). We showed that during the exposure pulse (10h) thiacloprid concentrations in the macrophyte-dominated section were 20-60% lower than those in the non-vegetated section. This suggests that spatial variability in contaminant concentrations, particularly in streams containing macrophytes, should be taken into account to enable a more realistic assessment of (i) exposure and associated effects and (ii) mass transport of pesticides and other chemicals into river systems (e.g. losses with surface runoff).     

Impaired terrestrial and arboreal locomotor performance in the western fence lizard (Sceloporus occidentalis) after exposure to an AChE-inhibiting pesticide

We examined the effects of a commonly used AChE-inhibiting pesticide on terrestrial and arboreal sprint performance, important traits for predator avoidance and prey capture, in the western fence lizard (Sceloporus occidentalis). Lizards were exposed to carbaryl (2.5, 25, and 250 microg/g) and were raced before and 4, 24, and 96 h after dosing. In the terrestrial setting, exposure to low concentrations of carbaryl had stimulatory effects on performance, but exposure to the highest concentration was inhibitory. No stimulatory effects of carbaryl were noted in the arboreal environment and performance in lizards was reduced after exposure to both the medium and highest dose of carbaryl. Our findings suggest that acute exposure to high concentrations of carbaryl can have important sublethal consequences on fitness-related traits in reptiles and that arboreal locomotor performance is a more sensitive indicator of AChE-inhibiting pesticide poisoning than terrestrial locomotor performance.     

Variation in sex steroids and phallus size in juvenile American alligators (Alligator mississippiensis) collected from 3 sites within the Kissimmee-Everglades drainage in Florida (USA)

This 3-year study was designed to examine variation in plasma sex steroids, phallus size, and the standard error (S.E.) associated with these endpoints in juvenile alligators collected from 3 sites within the Kissimmee-Everglades drainage (Florida, USA) with varying concentrations of sediment organochlorine contaminants. We hypothesized that decreased plasma sex steroid concentrations and phallus size would be observed in the higher contaminant site when compared to the intermediate and lower contaminant sites. Furthermore, we hypothesized that greater S.E. associated with these endpoints would be observed for the populations from more contaminated sites. We found that differences existed with females from the higher contaminant site exhibiting lower plasma estradiol-17beta (E2) and testosterone (T) concentrations. Males from the higher contaminant site exhibited smaller phallus sizes than males from the intermediate and lower contaminant sites. Smaller phallus size in this case differed from that reported in Lake Apopka male alligators [Gen. Comp. Endocrinol. 116 (1999) 356] in that a significant positive relationship between body size and phallus size existed. No difference among sites was observed in plasma T for males. Lower S.E. was associated with E2 and T concentrations in females from the higher contaminant site and in phallus size in males from the higher contaminant site. This pattern was opposite to what we had hypothesized. We concluded that variation in plasma E2 and T concentrations, phallus size, and the S.E. associated with these endpoints exists among the 3 sites with the patterns matching the patterns of organochlorine contamination, although S.E. patterns were opposite to what was predicted.     

Species- and stage-specific differences in trace element tissue concentrations in amphibians: implications for the disposal of coal-combustion wastes

Information on species- and stage-specific patterns of contaminant accumulation is generally lacking for amphibians, yet such information could provide valuable knowledge on how amphibians interact with contaminants. We assessed concentrations of As, Cd, Cu, Ni, Pb, Se, Sr, and Zn in whole bodies of larval, recently metamorphosed, and adult life stages in Bufo terrestris and Rana sphenocephala from a site that currently receives coal combustion waste (CCW) discharge, a site where CCW was formerly discharged that has undergone natural attenuation for 30 years, and a nearby reference site. For the majority of elements (As, Cd, Cu, Ni, Pb, Zn), concentrations were highest in larvae, but Se and Sr concentrations remained elevated in later life stages, likely because these elements are S and Ca analogs, respectively, and are thus retained throughout structural changes during metamorphosis. Element concentrations were generally higher in B. terrestris than in R. sphenocephala. Concentrations of As, Se, and Sr were up to 11-35 times higher in metamorphs emigrating from CCW-polluted wetlands compared to unpolluted wetlands, suggesting metamorphosed amphibians can transport trace elements from aquatic disposal basins to nearby uncontaminated terrestrial habitats. In addition, anurans utilizing naturally revegetated sites up to 30 years after CCW disposal ceases are exposed to trace elements, although to a lesser degree than sites where CCW is currently discharged.     

Contaminant-induced irreversible changes in properties of the soil-vadose-aquifer zone: an overview

Most studies on contaminant interactions with the subsurface environment focus on contaminant transport, retention and persistence, and on potential remediation of polluted soils, vadose zones and aquifers. Changes in the soil-vadose-aquifer zone (SVAZ) matrix and properties, caused by human activities, are thus usually considered to be deviations from a normal geochemical environment, which will disappear by natural processes or by specific remediation procedures. However, contaminants may also cause, under specific conditions, irreversible changes in SVAZ properties. In this critical overview, we discuss a different aspect of contaminant-SVAZ interactions: irreversible changes in natural SVAZ properties as a result of anthropogenically-induced chemical contamination. We survey selected research results that illustrate various aspects of such phenomena, in soils, aquifers and the vadose zone. Grouping contaminants according to major and trace elements, we observe that major elements can irreversibly affect water transmission and other physical and chemical properties of the SVAZ, mainly in the liquid phase, while trace elements affect mostly the solid phase matrix.     

Mercury exposure in terrestrial birds far downstream of an historical point source

Mercury (Hg) is a persistent environmental contaminant found in many freshwater and marine ecosystems. Historical Hg contamination in rivers can impact the surrounding terrestrial ecosystem, but there is little known about how far downstream this contamination persists. In 2009, we sampled terrestrial forest songbirds at five floodplain sites up to 137 km downstream of an historical source of Hg along the South and South Fork Shenandoah Rivers (Virginia, USA). We found that blood total Hg concentrations remained elevated over the entire sampling area and there was little evidence of decline with distance. While it is well known that Hg is a pervasive and long-lasting aquatic contaminant, it has only been recently recognized that it also biomagnifies effectively in floodplain forest food webs. This study extends the area of concern for terrestrial habitats near contaminated rivers for more than 100 km downstream from a waterborne Hg point source.     

Potential sources of methylmercury in tree foliage

Litterfall is a major source of mercury (Hg) and toxic methylmercury (MeHg) to forest soils and influences exposures of wildlife in terrestrial and aquatic ecosystems. However, the origin of MeHg associated with tree foliage is largely unknown. We tested the hypothesis that leaf MeHg is influenced by root uptake and thereby related to MeHg levels in soils. Concentrations of MeHg and total Hg in deciduous and coniferous foliage were unrelated to those in soil at 30 urban and rural forested locations in southwest Ohio. In contrast, tree genera and trunk diameter were significant variables influencing Hg in leaves. The fraction of total Hg as MeHg averaged 0.4% and did not differ among tree genera. Given that uptake of atmospheric Hg(0) appears to be the dominant source of total Hg in foliage, we infer that MeHg is formed by in vivo transformation of Hg in proportion to the amount accumulated.     

Endocrine disrupting chemicals accumulate in earthworms exposed to sewage effluent

Endocrine disrupting chemicals (EDCs) can alter endocrine function in exposed animals. Such critical effects, combined with the ubiquity of EDCs in sewage effluent and potentially in tapwater, have led to concerns that they could be major physiological disruptors for wildlife and more controversially for humans. Although sewage effluent is known to be a rich source of EDCs, there is as yet no evidence for EDC uptake by invertebrates that live within the sewage treatment system. Here, we describe the use of an extraction method and GC-MS for the first time to determine levels of EDCs (e.g., dibutylphthalate, dioctylphthalate, bisphenol-A and 17beta-estradiol) in tissue samples from earthworms (Eisenia fetida) living in sewage percolating filter beds and garden soil. To the best of our knowledge, this is the first such use of these techniques to determine EDCs in tissue samples in any organism. We found significantly higher concentrations of these chemicals in the animals from sewage percolating filter beds. Our data suggest that earthworms can be used as bioindicators for EDCs in these substrates and that the animals accumulate these compounds to levels well above those reported for waste water. The potential transfer into the terrestrial food chain and effects on wildlife are discussed.     

Groundwater flow velocities in karst aquifers; importance of spatial observation scale and hydraulic testing for contaminant transport prediction

Environmental Science and Pollution Research - We review scale dependence of hydraulic conductivities and effective porosities for prediction of contaminant transport in four UK karst aquifers....     

A conceptual framework for understanding illegal killing of large carnivores

The growing complexity and global nature of wildlife poaching threaten the survival of many species worldwide and are outpacing conservation efforts. Here, we reviewed proximal and distal factors, both social and ecological, driving illegal killing or poaching of large carnivores at sites where it can potentially occur. Through this review, we developed a conceptual social–ecological system framework that ties together many of the factors influencing large carnivore poaching. Unlike most conservation action models, an important attribute of our framework is the integration of multiple factors related to both human motivations and animal vulnerability into feedbacks. We apply our framework to two case studies, tigers in Laos and wolverines in northern Sweden, to demonstrate its utility in disentangling some of the complex features of carnivore poaching that may have hindered effective responses to the current poaching crisis. Our framework offers a common platform to help guide future research on wildlife poaching feedbacks, which has hitherto been lacking, in order to effectively inform policy making and enforcement.