长托宁治疗急性有机磷农药中毒的临床分析

目的观察长托宁救治急性有机磷农药中毒的疗效。方法（治疗组）80例和阿托品治疗组（对照组）80例,将两组疗效进行对比分析。将160例有机磷农药中毒患者分为长托宁治疗组结果治疗组在用药次数、胆碱酯酶活力（ChE）恢复程度、治愈时间等方面有显著优势。结论长托宁是治疗急性有机磷农药中毒的理想抗胆碱药,值得临床推广。     

长托宁、阿托品联合血液灌流治疗急性有机磷农药中毒伴呼吸衰竭的临床疗效

目的:探讨长托宁、阿托品联合血液灌流治疗急性有机磷中毒伴呼吸衰竭的临床疗效。方法:选取2009年1月至2016年8月在我院治疗的重症有机磷农药中毒伴呼吸衰竭患者68例,随机分为2组,A组35例,B组33例,A组患者接受长托宁联合阿托品常规治疗,B组患者接受长托宁、阿托品联合血液灌流治疗。比较两组患者治疗期间血清乙酰胆碱酶、肌酸激酶的含量以及治疗情况、临床疗效,同时记录治疗期间不良反应的发生情况。结果:治疗期间,B组患者血清Ach E的含量显著高于A组患者(P0.05),血清CK含量显著低于A组患者(P0.05);B组患者的清醒时间、到达阿托品化时间、中毒症状消失时间以及住院时间显著低于A组患者(P0.05);B组患者的病死率以及不良反应的发生率显著低于A组(P0.05);B组患者治疗的总有效率显著高于A组(P0.05)。结论:长托宁、阿托品联合血液灌流可以显著缓解急性有机磷中毒伴呼吸衰竭患者体内胆碱能紊乱的状态,提高临床疗效,并且有效降低治疗期间不良反应的发生率。     

不同剂量长托宁联合血液灌流在急性有机磷农药中毒中的应用效果观察

目的:研究不同剂量长托宁联合血液灌流在急性有机磷农药中毒中的应用效果。方法:选取急性有机磷农药中毒患者66例,随机分为A、B两组,每组33例。A组患者给予大剂量长托宁及血液灌流治疗,B组患者给予小剂量长托宁及血液灌流治疗。对比两组临床治疗效果及住院期间不良反应发生情况,对比两组治疗前后实验室指标变化。结果:A组治疗后意识恢复时间、中毒症状消失时间、胆碱酯酶(CHE)恢复时间、住院时间分别为(1.64±0.42)d、(4.84±1.25)d、(4.75±1.21)d、(5.43±1.88)d,显著少于B组的(2.10±0.82)d、(5.22±1.84)d、(5.67±1.92)d、(7.24±2.45)d(P0.05),且A组死亡率0.00%(0/33)显著低于B组的18.18%(6/33)(P0.05)。A组治疗后二氧化碳分压(PaCO2)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)水平分别为(38.71±1.44)mm Hg、(74.54±6.12)U/L、(24.45±3.57)U/L,显著低于B组治疗后的(42.43±2.45)mm Hg、(82.78±8.57)U/L、(30.12±5.75)U/L(P0.05),A组治疗后动脉血氧分压(PaO2)水平为(86.91±5.76)mm Hg,显著高于B组治疗后的(83.76±4.87)mm Hg(P0.05)。两组不良反应总发生率(18.18%VS 11.11%)对比无显著差异(P0.05)。结论:与小剂量用药相比,大剂量长托宁联合血液灌流治疗急性有机磷农药中毒疗效更好,且用药安全。     

血浆置换联合血液灌流治疗急性重度有机磷农药中毒的疗效及对患者肝功能的影响

目的:探讨血浆置换联合血液灌流治疗急性重度有机磷农药中毒的临床疗效及对患者肝功能的影响。方法:选择我院于2014年1月至2017年9月收治的66例急性重度有机磷农药中毒患者,按照随机原则分为血液灌流组(n=30)、联合治疗组(n=36),两组均接受急性重度有机磷农药中毒常规治疗,在此基础上血液灌流组和联合治疗组分别接受血液灌流、血浆置换联合血液灌流治疗。观察两组治疗效果,治疗前、治疗1周后血浆丙氨酸转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素水平。观察治疗过程中的不良反应发生情况。结果:与血液灌流组比较,联合治疗组胆碱酯酶(CHE)恢复时间较短,而长托宁用量较少,住院时间较短,且抢救成功率较高(P0.05)。治疗1周后联合治疗组血浆ALT、AST、总胆红素显著低于血液灌流组(P0.05)。治疗过程联合治疗组出现1例皮疹,不良反应发生率为2.78%,血流灌注组无不良反应发生,两组不良反应发生率比较无差异(P0.05)。结论:血浆置换联合血液灌流能明显提高急性重度有机磷中毒患者的抢救成功率,同时改善患者肝功能,且安全性良好,值得临床推广。     

长托宁治疗有机磷农药中毒临床观察

有机磷农药中毒是临床中常见的危重急诊,抢救不及时,常危及生命。我院自2004年1月~2006年12月,使用盐酸戊乙奎醚(长托宁)抢救有机磷农药中毒,疗效确切,毒副反应少,现报告如下。1临床资料1·1一般资料86例有机磷农药中毒患者,全部根据病史、症状、体征、胆碱酯酶检测确诊为有机磷农药中毒。将患者随机分为长托宁组(长托宁加氯磷定组)和对照组(阿托品加氯磷定组)。长托宁组46例,男20例,女26例,年龄12~70岁,平均34·5岁。其中自服中毒26例,误服中毒10例,皮肤和呼吸道接触中毒10例;轻度中毒8例,中度中毒25例,重度中毒13例。阿托品组40例,男18例,…     

阿托品与盐酸戊乙奎醚注射液联合治疗重度急性有机磷农药中毒的疗效分析

目的探讨阿托品联合盐酸戊乙奎醚注射液治疗重度急性有机磷农药中毒(AOPP)的临床疗效。方法选择2014年3月~2017年2月我院收治的116例重度AOPP患者为研究对象,随机均分为两组,对照组予以常规阿托品治疗,观察组在对照组的基础上联合盐酸戊乙奎醚注射液治疗,观察比较两组患者的临床疗效及用药安全性。结果观察组患者的阿托品化时间、中毒症状消退时间、CHE活力恢复70%以上时间、住院时间均短于对照组,差异有统计学意义(P0.05)。观察组患者的阿托品中毒率、中毒反跳率均低于对照组,差异有统计学意义(P0.05)。结论阿托品联合盐酸戊乙奎醚注射液治疗重度AOPP的临床疗效确切,可快速达到阿托品化,有助于短期内恢复CHE,促进中毒症状消退,减少抗胆碱药的毒副作用。     

长托宁治疗急性有机磷中毒的血常规动态观察

目的：探讨急性有机磷中毒患者（AOPP）血常规动态变化特点及长托宁的干预效果观察。方法：对我院34例急性有机磷中毒患者采用长托宁治疗,分析了入院第1,3,5,7,9天患者血液的各种指标动态变化趋势。结果：长托宁治疗后患者的血液指标均得到明显改善,治疗前后血液指标的差异有统计学意义（P〈0.05）;不同中毒程度各种血液指标的差异有统计学意义（P〈0.05）,其中除了白细胞数量（WBC）和中性粒细胞比例（N）在重度中毒患者中最高,而其他血液指标值在重度中毒患者中最低;对患者有无并发症的分析显示：两者之间除了平均红细胞体积（MCV）和血小板比积（PCT）的差异没有统计学意义（P〉0.05）其他各种血液指标的差异均有统计学意义（P〈0.05）。结论：长托宁对急性有机磷中毒的治疗效果好,不同中毒程度以及患者并发症的有无对血液指标有明显的影响,为急性有机磷中毒的病理变化提供理论基础,有助于临床病情的观察以及预后的判断。     

盐酸戊乙奎醚治疗有机磷中毒重度中间综合症临床研究

目的:探讨分析盐酸戊乙奎醚在急性有机磷中毒重度中间综合症治疗中的效果。方法:选取急性有机磷中毒并发重度中间综合症患者34例,依治疗方案分为对照组和观察组,各17例。2组患者基础治疗相同,对照组给予氯解磷定+阿托品+机械通气治疗;观察组采用氯解磷定+盐酸戊乙奎醚+机械通气治疗。观察比较2组患者肌无力症状消失时间、机械通气时间以及住院时间。结果:观察组患者肌无力症状消失时间、机械通气时间以及住院时间均明显低于对照组,经比较,差异均有统计学意义(P0.01)。结论:盐酸戊乙奎醚可快速有效缓解急性有机磷中毒重度中间综合症患者的临床症状,显著缩短病程。     

长托宁救治有机磷农药中毒的护理

2004年以来,我院急诊科采用了新型的抗胆碱药物盐酸戊乙喹醚(长托宁)取代阿托品,用于抢救有机磷农药中毒时,中毒症状消失快,治愈率高,毒副作用或不良反应少,使用方便,取得较好的疗效,现将临床护理报告如下。1临床资料1·1一般资料2004年至2006年4月,我院急诊科应用长托宁抢救有     

纳洛酮联合复方麝香注射液治疗急性酒精中毒128例疗效观察

目的观察复方麝香注射液联合纳洛酮治疗重度酒精中毒的临床疗效。方法将128例急性酒精中毒患者随机分为治疗组和对照组,治疗组66例,采用复方麝香注射液联合纳洛酮治疗;对照组62例,单纯采用纳洛酮治疗,并观察比较两组患者的清醒时间,症状缓解时间和不良反应情况。结果治疗组在恢复清醒时间,头痛头晕、恶心呕吐症状缓解时间,烦躁控制时间等方面均优于对照组(P0.001)。结论复方麝香注射液联合纳洛酮治疗急性酒精中毒疗效明显。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细