H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

H2O2诱导的肝星状细胞凋亡后胶原的变化

Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.     

AA-861抑制5-LO途径对KC和HSC的影响

Objective To investigate the effects of AA-861, the specific inhibitor of 5-lipoxyge-nase (5-LO), on the activation, proliferation and gene expression of kupffer cell (KC) and hepatic stellate cell (HSC). Methods 1) After being exposed to AA-861, the gene expression, proliferative ability and apoptosis of activated KC induced by lipopolysaecharide (LPS) were detected; 2) After the activated KC being exposed to AA-861, the supernatant of KC was added into quiescent HSC, then the proliferative ability and gene expression of quiescent HSC were observed. Results 1) After being activated by LPS, the mRNA and protein expression of 5-LO in KC increased remarkably while the mRNA and protein expression of 5-LO and 5-LO production decreased significantly after KC being ex-posed to AA-861. 2) After the supernatant of activated KC was added into quiescent HSC, the prolif-erative ability and gene expression of Collagen-1, α-SMA and TIMP-1 were increased significantly. However, after the supernatant of activated KC being exposed to AA-861 was added to quiescent HSC, it inhibited the activation of quiescent HSC and the proliferative ability and gene expression of HSC were decreased. Conclusion Inhibition of the 5-LO pathway by AA-861 can induce the over-pro-liferated KC to undergo apoptosis, and then inhibit the activation and proliferation of HSC and de-crease the production of ECM.     

二溴海因/二氧化硅复合粒子的制备及其杀菌性能评价

通过实验室制备，合成了二溴海因／二氧化硅复合粒子，运用扫描电镜（SEM）与红外光谱（IR）进行了表征，SEM结果显示，在SiO2的表面覆合了二溴海因，红外光谱图表明，复合粒子中二溴海因和二氧化硅之间以物理作用方式结合，将单纯二溴海因与二溴海因／二氧化硅复合粒子溶解于水，测定其在水中的释放速度，结果表明：二溴海因／二氧化硅复合粒子中的二溴海因在水中释放的速度明显慢于纯二溴海因，且能维持较长的作用时间，比较纯二溴海因与含等量二溴海因的复合粒子对大肠杆菌、金黄色葡萄球菌、白葡萄球菌和枯草芽孢杆菌的抑制效果，发现在二溴海因含量高于有效抑茵浓度时，复合粒子对茵体的抑制效果高于纯二溴海因。     

The effect of pH and PCO2 on epidural analgesia with 2% 2-chloroprocaine

Increasing the pH of local anesthetics with sodium bicarbonate has been reported to hasten their onset of action. The purpose of this study was to compare the onset and duration of epidural analgesia with the use of sodium bicarbonate and tromethamine to increase the pH of 2% chloroprocaine (2CP). Five groups of patients were studied: Group I received 2CP; Group II received 2CP buffered to a pH of 7.1 with tromethamine; Group III received 2CP buffered to a pH of 7.1 with sodium bicarbonate; Group IV received 2CP buffered to a pH of 7.7 with tromethamine; and Group V received 2CP buffered to a pH of 7.7 with sodium bicarbonate. The final pH and PCO2 of each solution were measured. Time to onset of analgesia was significantly delayed with either of the tromethamine buffered groups (II [5.6 +/- 1.0 minutes] and IV [5.4 +/- 0.4 minutes]) when compared with data from the unbuffered control (I [4.4 +/- 0.1 minutes]) and the sodium bicarbonate buffered (III [4.5 +/- 0.8 minutes] groups and Group V [2.7 +/- 0.9 minutes]). Only when sodium bicarbonate buffer adjusted to pH 7.7 (Group IV) was onset significantly more rapid than the unbuffered 2CP (I) and tromethamine buffered 2CP (II and IV). Multiple regression analysis revealed that onset times were significantly related to both pH and PCO2. The coefficient of determination for this model was 0.5156.(ABSTRACT TRUNCATED AT 250 WORDS)     

Evaluation of the association of IGF2BP2 variants with type 2 diabetes in French Caucasians

OBJECTIVE—We performed a comprehensive genetic association study of common variation spanning the IGF2BP2 locus in order to replicate the association of the “confirmed” type 2 diabetes susceptibility variants rs4402960 and rs1470579 in the French Caucasian population and to further characterize the susceptibility variants at this novel locus.RESEARCH DESIGN AND METHODS—We genotyped a total of 21 tagging single nucleotide polymorphisms spanning the IGF2BP2 locus in our type 2 diabetes case-control cohort comprising 3,093 French Caucasian subjects.RESULTS—IGF2BP2 variants rs4402960 and rs1470579 were not associated with type 2 diabetes in the present study (P = 0.632 and P = 0.896, respectively). Meta-analysis of genotype data from over 34,000 subjects demonstrated that our inability to replicate rs4402960/rs1470579 was consistent with the findings from several previous genome-wide association study (GWAS) datasets that were underpowered to detect this modest association signal (odds ratio [OR] 1.14). We obtained novel evidence that rs9826022, a borderline rare variant (5% minor allele frequency) in the 3′ downstream region, was associated with type 2 diabetes (P = 0.0002; OR 1.53 [95% CI 1.22–1.91]). This result was corroborated by the meta-analysis of 10,542 genotypes from the current study and GWAS datasets using both fixed (P = 9.47 × 10⁻⁶; 1.30 [1.16–1.46]) and random effects (P = 0.001; 1.30 [1.11–1.52)] calculations.CONCLUSIONS—We were unable to replicate the confirmed rs4402960/rs1470579 susceptibility variants but found novel evidence for a rare variant in the 3′ downstream region of IGF2BP2. Further genetic and functional studies are required to identify the etiological IGF2BP2 variants.The insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) gene on chromosome 3q27 is a paralog of IGF2BP1, a known regulator of IGF2 gene expression. Genome-wide association studies (GWASs) carried out by the Finland-U.S. Investigation of NIDDM Genetics (FUSION) (1), the Wellcome Trust Case Control Consortium (WTCCC) (2), and the Diabetes Genetics Initiative (DGI) (3) groups each found modest evidence that single nucleotide polymorphisms (SNPs) in the IGF2BP2 region are associated with type 2 diabetes. The subsequent meta-analysis of primary and replication datasets from these GWASs corroborated these findings and identified two strongly correlated IGF2BP2 variants, rs1470579 and rs4402960, as “confirmed” type 2 diabetes susceptibility variants (1–3). By contrast, the French/Canadian GWAS (4) typed 10 SNPs across the IGF2BP2 locus, including rs1470579, in 1,363 subjects, but found no nominal (P < 0.05) association signals at IGF2BP2. In an attempt to replicate the IGF2BP2 association findings in the French Caucasian population in a larger study and to further characterize the susceptibility variants at this novel locus, we performed an association study of HapMap Phase II tag SNPs spanning the IGF2BP2 locus in 3,093 French Caucasian subjects.