提高晋南牛肉用性能的杂交组合研究（二报）：杂交一代与晋南牛肥育性能测定

平均15月龄的夏晋、西晋、利晋杂交1代和晋南阉牛各4头，按美国NRC肉牛营养标准配合日粮，经100天育肥后屠宰（约18月龄）测定其肉用性能。复晋、西晋、利晋和晋南牛平均日增重分别是904．5±9．12，838．5±6.2，824．3±3．29和618．7±16．6克；每增重1千克活重消耗总可消化养分6．11，7.01，7.15和7.83千克；消耗粗蛋白质846．7，931．1，949．1和1047.9克；宰前活重分别为421．5±7.94，412，0±7．53，404.3±10．52和318．0±11．63千克；屠宰率平均分别为57．5．56．96，55．97和51．69％；肉骨比分别为4．65，4．35．5．12和4．1；体腔脂肪总重分别为15．85，11．15，11．70和17.35千克。结果表明，杂交牛肉用性能均有较大提高。     

不同杂交组合牛育肥试验及屠宰测定

在小哨示范牧场全日制放牧管理条件下，对23头513±10日龄、体重271±19千克、6个组合的杂交公牛进行育肥试验。结果：完全放牧育肥期平均日增重为0．56±0．34干克，其中：BNY0.58±0．22千克、BY0.73±0.3千克、BM0．5±0．29千克、MBM1．17±0.47千克、MY0．38±0．25千克、MMY0．33±0．2千克。放牧十补饲育肥期平均日增重为0．95±0．31千克（比不补饲提高69．9％），其中：BMY1．06±0．17千克、BY0．8±0．27千克、BM1．01±0．39千克、MBM1．07±0.46千克、MY0.74±0.2千克、MMY0.9±0.28千克。从综合育肥效果来看BMY较好，其次为MY和BY组合。MBM和BM组合虽然非常优秀，但由于不可能大量进口纯种莫累灰母牛，不适于在农村推广。从上述牛中选出有代表性的4个组合各1头进行屠宰测定，其结果平均屠宰率和净肉率分别为53．8±1．6％和43．4±1．7％f其中：MMY为55．1％，45．3％、MY55％、13．6％；BY53．5％、43．5％；BMY51．7％、41．1％。     

温岭高峰牛生产优质牛肉的潜力研究

为充分利用地方牛种资源和饲草饲料资源，本试验对放牧饲养到1岁的温岭高峰牛，采用氨化稻草、啤酒糟加少量精料的低成本肥育方法，进行6个月的舍饲育肥，开发优质牛肉生产。经6头牛试验，18月龄头均活重达380±32．7千克，头均日增重0．84千克；抽样屠宰测定3头，胴体重233．1±5．70千克，屠宰率60．05±1．37％，净肉率51．16±1．99％．胴体产肉率85.18±1．77％，眼肌面积85.3±16．6平方厘米。优质切块肉比35.47±1．05％,9－11肋间肉样含氨基酸44．02％。肉质鲜嫩、多汁，经宾馆餐厨师评议，达到优质牛肉指标。     

牛棉酚中毒的诊疗体会

2004年11月份，豫北曲沟养牛场饲养了24头牛．其中中国荷斯坦公牛9头．10～12月龄．平均体重340千克；夏洛来公牛与本地黄母牛杂交的R役肉兼用型幼牛10头，8～10月龄平均体重218千克，性别为6公4母：另5头为本地黄牛，其中1头公牛．12月龄．体重220千克，母牛8～9月龄．平均体重137千克。24头牛在同一牛舍饲养．饲草饲料相同，都是自配的饲料饲喂。     

大额牛,云南瘤牛屠宰性能研究

试验对小型大额平，云南瘤牛各4头公牛进行肉用性能研究，供试牛的平均年龄3．5±0．5岁。大额牛宰前活重149．0±19．0千克，胴体重78．18±8．82千克，净肉重59．28±7．92千克，屠宰率52．56±0．78％，净肉率39．76±0．24％，眼肌面积49．84±14．82厘米2；云南瘤牛宰前活重324．5±28．5千克，胴体重165．91±16．29千克，净肉重130．05±15．55千克，屠宰年51．08±1．53％，净肉率39．97±1．29％，眼队面积76．07±3．6厘米2。结果表明：两个品种在放牧条件下均具有较好的产肉性能和良好的肉质，是优良的地方牛种。     

营养补给剂—MCT810对哺乳仔猪死亡率的影响

选择胎次相同、体况及产期相近的母猪14头，随机分为试验组与对照组，试验组在仔猪出生后12小时内及12～36小时分别两次投喂乳猪用营养补给剂──MCT810，其它饲养管理条件与对照组一致。结果表明，MCT能降低仔猪（特别是弱小仔猪）的死亡率，7日龄内仔猪死亡率：初生重0．7千克以下的仔猪试验组为13．33％，对照组为35．00％；初生重0．7～1．0千克的仔猪试验组为500％，对照组为9．09％；初生重1．0千克以上的仔猪试验组为2．94％，对照组为3．45％。     

杜湖配套系商品瘦肉猪在南方的生产性能测定

对湖北白猪Ⅵ系196窝成年母猪商品生产产仔统计产仔数为12．03，35日龄断奶育成率为96．22％。对100头商品猪的肥育性能测定表明，从75日龄到60千克左右平均日增重747克；从60千克到90千克平均日增重为761克；整个测定期的平均日增重为754克；达100千克体重的日龄为170日。     

仔猪浓缩饲料饲养效果研究报告

本试验分两组，每组５０头。仔猪从初生到６０日龄结束。对照组喂自配常规饲料，试验组喂基础日粮加Ｐ－５－Ａ乳猪浓缩料。结果试验组平均体重１３．８５千克，日增重２０７克，料肉比０．９７：１；对照组平均体重１２．３５千克，料肉比０．９７：１；对照组平均体重１２．３５千克，日增重１８２克，料肉比１．２７：１。平均体重，日增重差异均极显著。经济效益试验组好于对照组。说明Ｐ－５－Ａ乳猪浓缩料有推广价值。     

有益菌制剂对提高仔猪成活率和断奶重的研究

利用北京好友巡天生物技术有限责任公司生产的抗体——微生态制剂“壮壮崽I号”和有益茵制剂“育崽宝”喂给初生仔猪和保育猪。仔猪断奶成活率达95．65％，21日龄断奶体重达7．6千克，断奶增重提高21．79％；保育猪65日龄体重达19．21千克，增重提高7．02％，料肉比降低了7．20％。下痢等发病数大幅度下降。     

两种不同营养水平的代乳粉对羔羊生长性能的影响

试验选用18只10日龄公羔，随即分成A、B、C三组，A组哺喂1号（低营养水平）代乳粉，B组哺喂2号（高营养水平）代乳粉，C组随母羊自然哺乳，每隔10d测定一次体重、体尺，进行了60d的饲养试验，结果表明：30日龄前，特别是10～20日龄阶段，自然哺乳组日增重达到154g／d，而代乳粉组平均日增重仅仅76．9g／d，试验组与对照组差异极显著（P〈0．01）；20日龄时，A、B、C三组体高增加分别为2．4％、6．50％、7．36％，A、B两组分别与C组差异极显著（P〈0．01）；体长A、B两组与C组差异显著（P〈0．05）：但随着日龄的增加，消化道逐渐健全，羔羊越来越习惯于吸允代乳粉，消化吸收能力加强，尤其到试验后期（41—60d），B组表现明显的生长优势，不同日龄体重、阶段平均日增重、体高、体长、胸围、管围都明显高于A组和C组。     

白术、微米白术和白术多糖对断奶仔猪生长性能和肠道形态及微生态区系的影响

试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。     

Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia

Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.     

Phase I evaluation of CCNU (lomustine) in tumor-bearing cats

1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.     

Prevalence of gastric lesions in racing Alaskan sled dogs

Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.     

Y‐chromosomal variation of local goat breeds of Turkey close to the domestication centre

Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.     

Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects

Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.     

Feline intracranial neoplasia: retrospective review of 160 cases (1985-2001)

The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.     

The Epidemiology of Gastrointestinal Nematodes of Dairy Cattle in Central Kenya

The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.     

Diversity of Ectoparasites in Sheep Flocks in Sa~o Paulo,Brazil

The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.     

A brief history of the prevention of infectious diseases by immunisations

Infectious diseases have always been a terrible scourge for humans. The appearance of these plagues, as they were called without distinction, was generally connected to various conditions: asters, climatic changes or religious reasons. The concept of contagious, and then infectious, diseases came slowly. Variolation, i.e. transmission of ‘virulent’ matter to induce a natural disease and the immunity against it, was brought from Constantinople to England by Lady Montague, in 1721. This ‘variolation’ technique was also often performed in veterinary medicine against diseases like sheep-pox or pleuropneumonia. As ‘vaccination’ is the term generally accepted for ‘immunisation’, variolation can be the word designating such a technique. The second period of the history of immunisation began, in 1880, with the studies of Pasteur and his collaborators. A great number of bacterial vaccines were developed: dead, live but attenuated or only parts of pathogens. The viruses were produced in animals, then in eggs and at last, in tissue cultures. Second generation vaccines appeared with genetic engineering: recombinant vaccines, vector vaccines, nucleic acids vaccines, and markers vaccines, among others. These novel technologies can permit the development of new ones and improve the quality of the vaccines already existing.