鹌鹑高尿酸血症模型建立及菊苣提取物对其影响的实验研究

目的塑造与人类代谢类似的高尿酸血症动物模型,观察中药菊苣提取物对其影响.方法选择鹌鹑作为模型动物,以酵母食饵为造模剂,塑造高尿酸血症模型,动态检测鹌鹑血清尿酸等生化指标,观察菊苣提取物对该模型动物血清尿酸水平等的影响.结果饲酵母食饵(15g/kg)7天,鹌鹑血清尿酸水平明显升高,持续5周.菊苣提取物小剂量给药7天可明显降低酵母诱发的鹌鹑血尿酸升高和对抗其一过性血糖的增高,给药14天其作用优于阳性对照药苯溴马龙.结论(1)该模型是筛选防治高尿酸血症中药及研究其机制较理想的动物模型.(2)菊苣提取物有明显的降尿酸作用.(3)造模机制和药物作用机理有待进一步深入研究.     

菊苣降尿酸作用的谱效关系研究

目的研究菊苣HPLC指纹图谱与其降尿酸药效的相关性,初步揭示菊苣降尿酸药效物质基础。方法高嘌呤饮食诱导鹌鹑高尿酸血症,并给予菊苣水提物。采用偏最小二乘(PLS)法将菊苣HPLC指纹图谱中各共有峰的相对峰面积和降尿酸药效进行数据处理,研究谱效相关性,确定菊苣降尿酸作用的药效物质基础。结果菊苣地上部分具有较好的降尿酸药效,根据变量投影重要性指标确定了菊苣HPLC指纹图谱中各个共有峰对降尿酸作用的贡献度。其中,绿原酸、菊苣酸及峰3、6、7、8所代表的化合物对降尿酸药效的贡献度较大。结论通过菊苣HPLC指纹图谱与其降尿酸药效PLS法分析可知,菊苣降尿酸作用与其所含的多种成分有关。     

菊苣的药理药效研究进展

目的:通过文献梳理,阐明其国内外研究进展,发现不足,为以后深入的研究奠定理论基础。方法:对近年来菊苣药理药效方面的研究的文献进行筛选、梳理和整合,分析研究的进展与不足。结果:菊苣药理药效方面的研究主要集中于菊苣治疗代谢性疾病的研究,还涉及其对钙吸收、肠道菌群等方面的影响,研究的涉及范围较广,但不够专。结论:菊苣在治疗代谢性疾病、调节肠道菌群等方面具有很好的效果,但其作用机制有待于进一步探讨。     

高尿酸血症相关靶点研究进展

高尿酸血症是因嘌呤代谢紊乱或尿酸排泄障碍所致的慢性代谢性疾病,与痛风、代谢综合征的发生关系密切.综述高尿酸血症相关作用靶点,为高尿酸血症的研究和治疗提供参考.     

菊苣干预高尿酸血症鹌鹑尿酸及相关代谢酶活性研究

目的探讨菊苣对高尿酸血症(HUA)鹌鹑尿酸及相关代谢酶活性的影响,阐释菊苣防治HUA的可能机制。方法将60只鹌鹑,按体质量随机分为5组,即正常组,模型组,苯溴马隆组(20 mg·kg-1·d-1),菊苣高、低剂量组(10,5 g·kg-1·d-1),每组12只。除正常组喂饲鹌鹑普通饲料外,其余各组均给予高嘌呤饲料(普通饲料拌入酵母干粉15 g·kg-1·d-1)复制HUA模型。以菊苣治疗28 d,动态观察鹌鹑尿酸(uric acid,UA)水平及UA生成代谢酶活性的变化。结果给药7,14,21,28 d,菊苣高、低剂量组可显著降低鹌鹑UA水平(P0.05);不同程度地抑制UA生成代谢酶5'-核苷酸酶(5'-NT)、腺苷脱氨酶(ADA)、嘌呤核苷磷酸化酶(PNP)、鸟嘌呤脱氨酶(GD)、黄嘌呤氧化酶(XO)的活性(P0.05,P0.01)。结论菊苣可有效降低鹌鹑HUA模型UA水平,可能与其降低UA代谢酶5'-NT、ADA、PNP、GD、XO的活性有关。     

菊苣防治高尿酸血症研究进展

随着社会的发展,当前人们的生活习惯发生了很大改变,快节奏的生活方式导致了人们不良生活习惯的增加,尤其体现在饮食方面,如饮食结构不合理、饮食不规律等。高尿酸血症患者也随之不断增多,并呈现年轻化趋势。临床常用降尿酸西药安全性不佳,有肝、肾功能损伤的风险。菊苣作为药食两用中药材,在高尿酸血症的治疗中可发挥重要作用。近年来的研究显示,菊苣有明显的降尿酸作用。通过对菊苣抑制尿酸产生和促进尿酸排泄的实验研究进行综述,探讨其治疗效果及作用机制,为开发菊苣降尿酸药物提供参考依据。     

菊苣提取物对鹌鹑血尿酸血脂的影响

目的研究菊苣提取物N2对高尿酸高甘油三酯血症鹌鹑血尿酸血酯的影响.方法用酵母高尿酸高甘油三酯血症鹌鹑模型,观察菊苣提取物N2对模型血清中尿酸、甘油三酯、黄嘌呤氧化酶、一氧化氮的作用.结果 与对照组相比,菊苣提取物N2三个剂量组血清尿酸,甘油三酯及一氧化氮水平明显降低,其高、中剂量还能降低血清黄嘌呤氧化酶活性.结论菊苣提取物N2具有降低高尿酸高甘油三酯血症鹌鹑血尿酸、血脂的作用.     

基于分子对接技术虚拟筛选菊苣与肠道CNT2结合的化学成分研究

利用分子对接技术虚拟筛选菊苣与肠道浓度型核苷转运蛋白2(CNT2)结合的化学成分,为探讨菊苣干预嘌呤核苷吸收的降尿酸作用机制研究提供理论依据。采用同源建模手段构建人CNT2三维结构模型,采用Vina软件虚拟筛选菊苣小分子化合物作用于CNT2的化学成分。以CNT2抑制剂7,8,3'-三羟基黄酮的打分为阈值,筛选出23个打分高于阳性抑制剂的菊苣化学成分。其中打分靠前的菊苣化合物是菊苣降尿酸作用的重要化合物,其能否通过抑制CNT2活性干预肠道嘌呤核苷的吸收降低体内尿酸水平有待生物学实验进一步探讨。CNT2可能是菊苣降尿酸的效用靶点,为指导实验研究菊苣干预嘌呤核苷吸收降尿酸研究提供向导。     

食疗与痛风

痛风是一种慢性代谢性疾病,是由于嘌呤代谢紊乱和(或)尿酸排泻减少所引起的一种与饮食结构有密切关系的疾病.其主要特点是体内尿酸产生过多,或肾脏排泄尿酸减少,从而引起血尿酸升高,沉积于肢体,而致红肿热痛,功能障碍.     

基于“肠-肾”途径的健脾祛湿中药抗痛风病研究

目的:从“肠-肾”途径探讨健脾祛湿中药防治痛风病作用。方法:10%果糖塑造大鼠高血尿酸模型,高钙高嘌呤食饵并限水诱导鹌鹑尿酸盐沉积模型,以菊苣、参苓白术散为示例药。生化检测大鼠血清尿酸(SUA)、粪便尿酸(FUA)水平,计算肾脏尿酸清除率(RCUA);检测鹌鹑粪尿中尿酸含量,计算尿酸排泄量。免疫组化分析大鼠肠道ABCG2、肾脏URAT1、OAT1,及鹌鹑肠道Occludin、肾脏p-p65表达。六胺银染色分析尿酸盐沉积。结果:1)菊苣可显著降低模型大鼠SUA、增加FUA、RCUA,促进肠道ABCG2表达,减少肾脏URAT1表达。2)参苓白术散可显著减少模型鹌鹑肾脏尿酸盐沉积,增加尿酸排泄量,增加肠道Occludin表达,减少肾脏p-p65表达。结论:痛风病发生发展中存在“肠-肾”途径的病理改变,健脾祛湿中药通过调节“肠-肾”尿酸转运促尿酸排泄、改善肠道屏障功能及肾脏炎症状态以祛除尿酸盐沉积,共同发挥防治痛风病作用。     

痛风病中药效用机制研究平台构建及应用

目的:建立中药防治痛风病的药效评价平台,为评价中药防治痛风病的药效特点与作用机制提供技术支撑.方法:集成"成分-疾病"靶标网络分析、病证结合动物模型、器官模型、细胞模型、分子生物学5个层次,从多层次系统构建中药防治痛风病的药效评价平台.结果:本课题组成功构建了基于尿酸生成与排泄、尿酸盐沉积以及沉积致炎的"整合论治"痛风...     

Anti-hepatotoxic effects of root and root callus extracts of Cichorium intybus L

The natural root and root callus extracts of Cichorium intybus were compared for their anti-hepatotoxic effects in Wistar strain of Albino rats against carbon tetrachloride induced hepatic damage. The increased levels of serum enymes (aspartate transaminase, alanine transaminase) and bilirubin observed in rats treated with carbon tetrachloride were very much reduced in the animals treated with natural root and root callus extracts and carbon tetrachloride. The decreased levels of albumin and proteins observed in rats after treatment with carbon tetrachloride were found to increase in rats treated with natural root and root callus extracts and carbon tetrachloride. These biochemical observations were supplemented by histopathological examination of liver sections. Results of this study revealed that Cichorium intybus root callus extract could afford a better protection against carbon tetrachloride induced heptocellular damage as compared to the natural root extract.     

Antimalarial activity of lactucin and lactucopicrin: sesquiterpene lactones isolated from Cichorium intybus L

Folklore reports from Afghanistan prior to the wars described the use of aqueous root extracts of Cichorium intybus (L.) as a light-sensitive plant remedy for malaria. Preparative isolation and bioassay against HB3 clone of strain Honduras-1 of Plasmodium falciparum identified the previously known light-sensitive sesquiterpene lactones Lactucin and Lactucopicrin to be antimalarial compounds.     

Anti-diabetic effects of Cichorium intybus in streptozotocin-induced diabetic rats

The present study was designed to investigate the hypoglycemic and hypolipidemic properties of an ethanolic extract of Cichorium intybus (CIE) which is widely used in India as a traditional treatment for diabetes mellitus. Male Sprague-Dawley rats aged 9 weeks (160-200 g) were administered with streptozotocin (STZ, 50mg/kg) intraperitoneally to induce experimental diabetes. The Cichorium intybus whole plant was exhaustively extracted with 80% ethanol, concentrated at 40 degrees C using a rotavapor and freeze dried to get powder. Hypoglycemic effects of CIE were observed in an oral glucose tolerance test (OGTT) in which, a dose of 125 mg of plant extract/kg body weight exhibited the most potent hypoglycemic effect. Moreover, daily administration of CIE (125 mg/kg) for 14 days to diabetic rats attenuated serum glucose by 20%, triglycerides by 91% and total cholesterol by 16%. However, there was no change in serum insulin levels, which ruled out the possibility that CIE induces insulin secretion from pancreatic beta-cells. In addition, hepatic glucose-6-phosphatase activity (Glc-6-Pase) was markedly reduced by CIE when compared to the control group. The reduction in the hepatic Glc-6-Pase activity could decrease hepatic glucose production, which in turn results in lower concentration of blood glucose in CIE-treated diabetic rats. In conclusion, our results support the traditional belief that Cichorium intybus could ameliorate diabetic state.     

Chemical constituents of Cichorium intybus L.]

OBJECTIVE: To investigate the chemical constituents of Cichorium intybus. METHOD: Constituents were separated by means of solvent extraction and chromatography on silical gel. The structures were established by spectral analysis and chemical evidence. RESULT: Seven compounds were isolated from the roots of C. intybus and four of them were identified as alpha-amyrin, taraxerone, baurenyl acetate and beta-sitosterol. CONCLUSION: All the four compounds were obtained from the plant for the first time.     

Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi

Screening was done of some plants of importance in the Ayurvedic system of traditional medicine used in India to treat enteric diseases. Fifty four plant extracts (methanol and aqueous) were assayed for their activity against multi-drug resistant Salmonella typhi. Strong antibacterial activity was shown by the methanol extracts of Aegle marmelos, Salmalia malabarica, Punica granatum, Myristica fragrans, Holarrhena antidysenterica, Terminalia arjuna and Triphal (mixture of Emblica of fi cinalis, Terminalia chebula and Terminalia belerica). Moderate antimicrobial activity was shown by Picorhiza kurroa, Acacia catechu, Acacia nilotica, Cichorium intybus, Embelia ribes, Solanum nigrum, Carum copticum, Apium graveolens, Ocimum sanctum, Peucedanum graveolens and Butea monosperma.     

朴春丽教授解毒通络调肝降浊方治疗糖尿病合并高尿酸血症经验

随着社会的飞速发展,人民生活水平的日益提高,糖尿病等代谢性疾病的发病率也随之日益增加,其中糖尿病合并高尿酸血症的高发生率引起人们的重视.目前,西医单纯降糖、降尿酸治疗常使病情反复,血糖、尿酸水平的波动往往增加痛风、心脑血管疾病发生的风险,且有一定的毒副作用.朴春丽教授根据多年的临床经验,针对这一情况,常运用解毒通络调肝...     

中医药治疗痛风的研究进展

痛风是单钠尿酸盐沉积在关节所致的晶体相关性关节病,与嘌呤代谢紊乱和（或）尿酸排泄障碍所致的高尿酸血症直接相关,除关节损害,还可伴发肾脏病变及其他代谢综合征的表现,临床常见的代谢性风湿病。近年来关于中医药治疗痛风的研究越来越多,包括中药单体、单味药、中药复方和中医外治法,其具有疗效显著且无明显不良反应的优势。通过对痛风的病因病机、中医药治疗的基础研究和临床研究等方面进行全面综述,为临床治疗提供理论依据。     

菊苣的药理药效研究及开发前景

菊苣Cichorium intybus L.是菊科菊苣属多年宿根植物.菊苣的主要成分有糖类、有机酸类、生物碱类、三萜类、倍半萜类和香豆素等;其药效研究主要有降血糖、降血脂、降尿酸作用、保肝作用以及对消化系统和心血管系统的影响作用,并在促进对钙的吸收利用,提高机体免疫力,抗过敏、抗菌、抗病原微生物方面有一定的作用;目前对药效利用主要用于保健饮品、功能性食品和特效药品进行开发,未来在特效药品开发方面极具开发价值和利用前景.