共查询到19条相似文献,搜索用时 375 毫秒
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银环蛇中毒死亡鉴定方法的实验研究——活化长碳臂生物素:夹心ABC-ELISA检测银环蛇毒 总被引:2,自引:0,他引:2
用自制活化长碳臂生物素标记马抗银环蛇毒抗体进行夹心ABC—ELISA测试,所获参数为:最小检出量0.25ng/ml;标准曲线最适检测范围0.25~250ng/ml;批内变异<8%,批间变异<16%,不同浓度回收率为78~89%;与同科眼镜蛇毒无交叉反应。作者从法医学鉴定的实际需要出发,利用正交设计研究了各种因素对检测的影响,结果示:蛇咬死亡动物血液和肾脏为最佳检材,最长检出时间可达30天。本方法常规应用可靠性强。 相似文献
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生物素掺入反向杂交法在法医学中应用的研究 总被引:1,自引:0,他引:1
建立了一套快速、准确检测人类 HLA- DQA基因的反向杂交检测技术,可准确判定 HLA- DQA基因位点的 6个等位基因即 0101、 0102、 0103、 0201、 0301、 0401。调查了中国北方汉族人群中 200例无关个体的 HLA- DQA基因频率及基因型的频率分布,杂合度 (H)为 0.75,个体识别能力 (DP)为 0.928。采用生物素直接掺入 PCR扩增的方法, 1ng的 DNA样品即可准确判型。对血液、血斑、精斑、精液与阴道液的混合斑、肌肉组织等检材进行检测,效果良好。在刑事案件及亲子关系鉴定中应用,准确地判定了检材的 HLA- DQA基因型,为侦察破案提供了科学依据。将此项技术商品化,完成了试剂盒的研制。 相似文献
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<正> 现分离出三种抗百草枯单克隆抗体,分别命名为APM-1,APM-2,和APM-3。为了评价这些单克隆抗体辩认各种类型的双吡啶基除草剂和百草枯的同类物的能力,我们建立了用抗生物素——生物素复体(ABC)的酶联免疫吸附检测的方法(ELISA)。这三种抗体对 相似文献
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应用本实验室自制的酶标记Glm(3)单克隆抗体和Det-ELISA方法.对北京地区汉族人群Glm(3)基因分布频率进行调查。1材料与方法1.1血样采集北京地区301名无血缘关系的健康人的静脉血,置4℃冰箱保存。临用前20倍稀释,同时20倍稀释抗体。1.2主要试剂酶标记抗Glm(3)单克隆抗体〔HRP-mcAb-Glm(3)〕,为本实验室自制;显色液:4-氯-1.奈酚(Fluka公司)和DAB混合物。1.3检测方法按文献[1]方法检测Glm(3)。2结果和讨论2.IGIm(3)的表型判断在硝酸纤维膜上呈现蓝褐色斑点为Glm(3)阳性。2.2北京地区汉族人群Glm(3… 相似文献
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血制和酶型等遗传标记在法医学个人识别与亲权鉴定中有着广泛的应用,目前,一般用免疫学方法和电泳技术对其进行测定,前者需用抗血清,其来源有二:1)天然抗体的分离,如抗-A 抗体可从螺丝和B 型血清中提取.抗-B 抗体可从霉菌和 A 型血清中提取,抗-H 抗体可以从鳝鱼血和荆豆种子浸出液中提取,抗-P 抗体可从山羊、猪等动物的血清中分离得 相似文献
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<正> 在生物学研究方面,免疫微体技术已被广泛地用于标记细胞表面的受体。本文描述了偶合于微体的抗体在法医ABO血型中的应用。微体(Estapor Latex PSI 496,2.9μm diameter,Rhone-Poulenc)部分地同纯化抗体偶联。该纯化抗体是在含有1%牛血清白蛋白的磷 相似文献
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Monoclonal antibody mouse antihuman semen-5 (MHS-5) (immunoglobulin G1 [IgG1]) was biotinylated using N-biotinyl-w-aminocaproic acid-N-hydroxysuccinimide ester. This monoclonal antibody-biotin conjugate recognized low molecular weight peptide bands between 10.5 and 20 kilodaltons on immunoblots of liquefied semen. Immunodominant peptides had molecular weights of 10.5, 11.5, and 13.5 kilodaltons. An enzyme-linked immunosorbent assay (ELISA) developed with the biotinylated-MAb and streptavidin peroxidase demonstrated sensitivity curves with lower limits of 10 ng of seminal fluid protein per microtiter well using 50 ng per well of monoclonal antibody-biotin conjugate. Cross-reactivity studies on a panel of human biological fluids and tissues demonstrated no cross-reactivity or false positives using the monoclonal antibody-biotin conjugate. The sensitivity of the monoclonal antibody-biotin ELISA was compared to ELISA based upon a polyclonal secondary antibody-peroxidase conjugate. These findings indicate that this ELISA assay, based on a biotinylated monoclonal antibody to a seminal vesicle-specific antigen, may be useful for semen identification. 相似文献
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Collins M Heagney A Cordaro F Odgers D Tarrant G Stewart S 《Journal of forensic sciences》2007,52(4):898-903
Five 44 gallon drums labeled as glycidyl methacrylate were seized by the Australian Customs Service and the Australian Federal Police at Port Botany, Sydney, Australia, in December 2004. Each drum contained a white, semisolid substance that was initially suspected to be 3,4-methylenedioxymethylamphetamine (MDMA). Gas chromatography-mass spectroscopy (GC/MS) analysis demonstrated that the material was neither glycidyl methacrylate nor MDMA. Because intelligence sources employed by federal agents indicated that this material was in some way connected to MDMA production, suspicion fell on the various MDMA precursor chemicals. Using a number of techniques including proton nuclear magnetic resonance spectroscopy ((1)H NMR), carbon nuclear magnetic resonance spectroscopy ((13)C NMR), GC/MS, infrared spectroscopy, and total synthesis, the unknown substance was eventually identified as methyl 3-[3',4'(methylenedioxy)phenyl]-2-methyl glycidate. The substance was also subjected to a published hydrolysis and decarboxylation procedure and gave a high yield of the MDMA precursor chemical, 3,4-methylenedioxyphenyl-2-propanone, thereby establishing this material as a "precursor to a precursor." 相似文献
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Besides the cannabinoid mimetic JWH-073, a novel 4 methylnaphthoyl homologue of JWH-073 was detected in a herbal mixture. The structure of the compound was elucidated after thin layer chromatographic enrichment from the herbal mixture by nuclear magnetic resonance (NMR) and gas chromatographic mass spectrometric (GC-MS) analysis. The paper outlines data after GC-MS, liquid chromatography mass spectrometry (LC-MS) and NMR spectroscopy, and describes the structure elucidation. 相似文献
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Analogs and derivatives of traditional illicit drugs are ever increasing in variety and creativity. Staying abreast of the new developments is a constant challenge for every forensic laboratory. Recently, a seizure from Australian Customs Service presented our laboratory with the designer cathinone 3,4-dimethylmethcathinone (3,4-DMMC). Gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), nuclear magnetic resonance (NMR) spectroscopy, infrared (IR) spectroscopy, and ultraviolet (UV) spectrophotometry were employed to analyze the spectroscopic characteristics of this cathinone. As an analog, 3,4-DMMC exhibits similar if not identical IR and UV profiles to mephedrone (4-MMC) and methcathinone; however, the retention time from GC is unique as expected, and the electron impact fragmentation pattern is consistent with the fragmentation pattern of other cathinones. The chemical shifts of the carbons and hydrogens were assigned by both one- and two-dimensional NMR techniques, while the molecular weight was confirmed by LC/MS. 相似文献
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胶体金免疫层析一步法快速检测G1m(3)因子 总被引:1,自引:0,他引:1
建立一种简便快速的胶体金免疫层析一步法 ,用于检测 G1m(3)因子。采用柠檬酸三钠还原法制备胶体金颗粒 ,标记抗人 G1m(3)单克隆抗体 ,研制出抗人 G1m(3)因子免疫层析检测试剂盒。样品的 G1m(3)因子 ,与测试条上的金标记抗体结合后沿着反应膜移动 ,再与膜上固相抗体结合形成肉眼可见的红色反应带。使用该方法可检出 10万倍稀释的血清样品 ,整个试验只需 5 min完成。对常见的 2 3种动物血 (痕 )检验 ,未出现交叉反应。在 10 0例样品的检测中 ,本方法的检测结果 ,与 Dot- EL ISA的检测结果的符合率为 10 0 %。该方法适用于法医物证快速检验。 相似文献
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A market study of 40 different green spray paints was carried out using infrared (FTIR) and Raman spectroscopy. The infrared technique distinguished between the 12 main groups based on their binder and extender composition. After visual comparison of the spectra 22 subgroups were observed. Raman spectroscopy was also carried out on the 40 reference paints in order to determine the pigment content. Analyses were undertaken using two different excitation sources: Argon ion (514.5 nm) and Helium-Neon (632.8 nm). The first generated strong fluorescence for most of the samples and created eight groups. Using the red laser, 15 classes were observed. Finally, using an analytical sequence starting with infrared spectroscopy followed by Raman Helium-Neon and then by Raman Argon laser, most of the paints were differentiated. In this study infrared and Raman spectroscopy complemented each other. FTIR supplied information about the binder and some extenders, and Raman provided information on the main organic pigments present. 相似文献
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Headspace solid-phase microextraction (HS-SPME) is assessed as an alternative to liquid-liquid extraction (LLE) currently used for 3,4-methylenedioxymethampethamine (MDMA) profiling. Both methods were compared evaluating their performance in discriminating and classifying samples. For this purpose 62 different seizures were analysed using both extraction techniques followed by gas chromatography-mass spectroscopy (GC-MS). A previously validated method provided data for HS-SPME, whereas LLE data were collected applying a harmonized methodology developed and used in the European project CHAMP. After suitable pre-treatment, similarities between sample pairs were studied using the Pearson correlation. Both methods enable to distinguish between samples coming from the same pre-tabletting batches and samples coming from different pre-tabletting batches. This finding emphasizes the use of HS-SPME as an effective alternative to LLE, with additional advantages such as sample preparation and a solvent-free process. 相似文献
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Andrzej Kwiatkowski M.Eng. Małgorzata Czerwicka Ph.D. Janusz Smulko Ph.D. Piotr Stepnowski Ph.D. 《Journal of forensic sciences》2014,59(5):1358-1363
Illegal alcoholic beverages are often introduced into market using cheap technical alcohol, which is contaminated by denatonium benzoate (Bitrex) of very small concentration. Bitrex is the most bitter chemical compound and has to be removed before alcohol consumption. The home‐made methods utilize sodium hypochlorite to disintegrate particles of denatonium benzoate in alcohol and to remove bitter taste before trading. In this experimental studies, we propose a novel method that detects in a fast way the remnants of denatonium benzoate in dubious alcohol samples by Raman spectroscopy. This method applies a portable Raman spectrometer of excitation wavelength 785 nm and utilizes the effect of surface‐enhanced Raman spectroscopy (SERS) to recognize the suspected alcoholic beverages. High effectiveness (over 98%) of YES/NO classification of the investigated samples was observed when the nonlinear algorithm support vector machine (SVM) was exploited at carefully adjusted detection parameters. The method can identify illicit alcohol within minutes. 相似文献
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Identification and quantitation of gamma-hydroxybutyrate (NaGHB) by nuclear magnetic resonance spectroscopy 总被引:2,自引:0,他引:2
The most common means of identification of gamma-hydroxybutyrate (NaGHB) involves using Fourier transform infrared spectroscopy (FTIR) or gas chromatography-mass spectrometry (GC-MS) of a suitable derivative. However, these methods may be complicated by possible shifts in chemical equilibrium between gamma-hydroxybutyric acid (GHB), GHB salts and the precursor lactone, gamma-butyrolactone (GBL). This paper addresses the technique of proton and carbon nuclear magnetic resonance spectroscopy (1H and 13C NMR) for the direct and accurate identification of GHB and GBL. The application of 1H NMR for GHB quantitation is also discussed. 相似文献