枸杞子粗提物对小鼠T淋巴细胞的免疫增强作用

枸杞子粗提物(简称LBP)5～50mg/kg/天×7天,ip,显著增加正常小鼠的脾重和提高外周血T淋巴细胞百分数。LBP5和10mg/kg对胸腺重量无明显影响,增加剂量到25～50mg/kg可使胸腺重量降低。LBP50～200mg/kg增强~3H-TdR掺入胸腺细胞的数量,在5～100mg/kg时,又可增强由ConA诱导的淋巴细胞增殖反应。在ConA一般浓度(5～40μg/ml)时,LBP25、50和100mg/kg对胸腺淋巴细胞增殖均有增强作用;在高浓度GonA60μg/ml时,大剂量LBP比低剂量对胸腺淋巴细胞增殖反应具有更强的作用。这些结果说明,在适宜剂量范围,LBP可以增强T淋巴细胞的增殖和作为一种免疫增强剂。     

促有丝分裂原对辐射引起人T淋巴细胞间期死亡的防护效应

辐射引起人T淋巴细胞的间期死亡可因用植物血凝素(PNA)和刀豆素A(Con A)处理而得到保护。先把T淋巴细胞和T细胞亚群(即早T和晚T细胞)同能粘附于玻璃的单核细胞分开,继而用lsopa-que-Ficoll梯度分离法和玫瑰花形成法的联合技术进一步提纯。提纯的T淋巴细胞,先用1%的PHA-M,5μg/ml的Con A或10μg/ml的脂多糖(LPS)处理,然后以0～600伦剂量进行照射,再置于CO_2培养箱中孵育。根据核固缩变化来测定淋巴细胞的死亡,并在照射后20小时时,测定其活存部分。据观察,用PHA处理过的T淋巴细胞的培养物中,细胞活存数比对照培养物高出15～20%。在早T和晚T细胞亚群中,均可看到PHA防护淋巴细胞间期死亡的效应。在辐射引起核固缩变化上,用Con A处理的防护效应,晚T细胞比早T细胞亚群更明显。LPS没有防止早T细胞亚群间期死亡的效应。     

消炎痛对小鼠造血组织的辐射防护作用

作者探讨消炎痛(INDO)对造血组织的辐射防护作用及其机理.方法:1.小鼠(雌性,C3Hf/kam,3月龄)受250kV的X线全身照射6.6～8.9Gy,剂量率为1.7Gy/min,分别测定INDO组(照前饮用35μg/ml INDO溶液6天)与对照组的LD50/30)2.小鼠X线照射6.5～9.5Gy,分INDO组(照前或照后饮用INDO溶液1～6天)与对照组,照前30min均腹腔注射WR-2721(400mg/kg),照后第9天测定内源脾结节数;3.INDO组(饮用INDO溶液6天)与对照组小鼠各照射1.5、2、3、4、5及6Gy,照后立即制备双侧股骨骨髓细胞悬液,以2×10~4～2.8×10~6的细胞数静脉注射给受8.5Gy照后1天的小鼠,注射后8天测定外源脾结节数.结果:1.INDO组与对照组内源脾结节数均随     

Study of the effects of Moerella iridescens polysaccharide on hepatitis B virus transfected HepG2 cells

目的 研究彩虹明樱蛤多糖(MIP)对转染HBV的HepG2细胞表达功能的影响.方法 以不同浓度( 10-2～ 104μg/ml) MIP作用于体外培养的HepG2.2.15细胞,采用MTT法、时间分辨免疫分析法(TRHA)和ELISA法检测MIP对HepG2.2.15细胞的毒性作用及HBsAg、HBeAg、Pre-S1Ag表达的影响,采用FQPCR检测MIP对HBV DNA复制的抑制作用.结果 MIP在10-2～102μg/ml浓度范围内对HepG2.2.15细胞无毒性作用,TC50为4633μg/ml.MIP在10-2～102μg/ml浓度范围内,对HBsAg、HBeAg、Pre-S1Ag以及HBV DNA均有一定抑制作用,相应的治疗指数分别为129、28、20和83.结论 MIP具有一定的抗HBV活性.     

低剂量X射线增强丝裂原诱导大鼠脾细胞增殖效应

实验选用8周龄F344/NSIC大鼠固定在2mm厚的丙烯笼内,置于距照射源40cm的照射台上进行全身照射,剂量率分别为0.01、0.025,0.05,0.1和0.2Gy/分,照射时间为1、5和10分钟,同时设空白对照组.照射剂量以大鼠背部皮肤量计算.在照射不同时间将大鼠乙醚麻醉取出胸腺、脾脏,分别置于盛有MEM液的陪替氏培养皿中制成细胞悬液,台盼蓝染色计数后离心,从MEM中移入含有10%小牛血清的RPMI 1640培养液中,最终浓度为5×10~6个/ml.在96孔平皿内加100μl含2倍浓度促细胞分裂剂的1640培养液,37.5℃、5%CO_2条件平衡后,向每孔内加100μl细胞悬液.上述条件下培养第44h加入~3H-TdR、48h收集细胞,液闪计数仪测~3H-TdR掺入量,时间为1分钟.结果如下:     

优化胞质分裂阻滞实验方案用于核质桥分析的可行性

目的探索优化胞质分裂阻滞实验方案用于辐射诱导核质桥分析的可行性,为以核质桥为指标进行生物剂量估算提供科学依据。方法用2 Gy ⁶⁰Coγ射线(剂量率为1 Gy/min)照射人离体外周血(设0 Gy对照),照射后28 h在细胞样品中加入终浓度为6μg/ml的松胞素B,培养48、56、68和72 h后收获;或照射后加入终浓度为0.6、1、2、6、10μg/ml的松胞素B,培养68 h后收获。应用胞质分裂阻滞法进行标本制备,分析单核细胞、双核细胞、多核细胞的比例,以及辐射诱导核质桥率及微核率。结果对不同细胞培养时间,随细胞培养时间的增加,0和2 Gy核分裂指数和双核细胞比例具有升高的趋势;2 Gy核质桥率无明显变化规律(0.0230~0.0330/细胞),差异无统计学意义(P>0.05),微核率差异亦无统计学意义(P>0.05)。对不同浓度松胞素B处理组,随松胞素B浓度的增加,0和2 Gy时核分裂指数和双核细胞比例有所升高;2 Gy核质桥率无明显变化规律(0.0230~0.0470/细胞),差异无统计学意义(P>0.05);与6μg/ml组相比,10μg/ml组微核率显著降低(U=2.74,P<0.01)。结论不同细胞培养时间组和不同松胞素B浓度组的辐射诱导核质桥率差异无统计学意义。适当缩短细胞培养时间可提前得到核质桥分析结果;培养开始加入松胞素B可简化实验步骤,但可供分析的细胞数过少,用于剂量估算的可行性尚需进一步研究。     

彩虹明樱蛤多糖对转染HBV的HepG2细胞表达功能的影响

目的研究彩虹明樱蛤多糖(MIP)对转染HBV的HepG2细胞表达功能的影响。方法以不同浓度(10-2~104μg/ml)MIP作用于体外培养的HepG2.2.15细胞,采用MTT法、时间分辨免疫分析法(TRFIA)和ELISA法检测MIP对HepG2.2.15细胞的毒性作用及HBsAg、HBeAg、Pre-S1Ag表达的影响,采用FQ PCR检测MIP对HBV DNA复制的抑制作用。结果 MIP在10-2~102μg/ml浓度范围内对HepG2.2.15细胞无毒性作用,TC50为4633μg/ml。MIP在10-2~102μg/ml浓度范围内,对HBsAg、HBeAg、Pre-S1Ag以及HBV DNA均有一定抑制作用,相应的治疗指数分别为129、28、20和83。结论 MIP具有一定的抗HBV活性。     

熊胆对HL-60细胞系的分化诱导作用

本文首次报道熊胆对人早幼粒白血病细胞系HL-60的分化诱导作用.HL-60细胞(1.6×10~5/ml)在含有熊胆(400μg/ml)的RPMI 1640培养液中37℃孵育5天左右,80%以上的细胞分化为具有单核-巨噬细胞特征的细胞.细胞增殖明显受到抑制.分化的细胞具有还原硝基蓝四唑和贴壁能力,显示吞噬乳胶颗粒的功能,并基本失去自发形成集落的能力.细胞的α-萘酚醋酸酯酶(α-NAE)和酸性磷酸酶(ACP)活性显著增加.     

低浓度超顺磁性氧化铁标记兔骨髓间充质干细胞的体外磁共振成像研究

目的:以浓度为25μg Fe/ml的超顺磁性氧化铁纳米粒子(SPIO)体外标记兔骨髓间充质干细胞(BMSCs),并探讨1.5 T核磁共振仪成像的特征和成像所需最低标记细胞浓度,以及在标记后1 d、1周、2周、3周、4周的信号变化特征。方法:分离、纯化、培养兔BMSCs并以25μg Fe/ml的SPIO培养液浓度标记,对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察,并进行MR成像,测量不同序列下不同浓度标记细胞管的信号强度,以确定扫描敏感序列及成像所需最低标记细胞浓度;再测量不同细胞浓度不同时相信号强度,来观察信号强度随时间变化的规律,并进行统计学分析。结果:浓度为25μg Fe/ml的超顺磁性氧化铁纳米粒子标记BMSCs的有效率接近100%,普鲁士蓝染色见细胞浆内有大小不等的蓝染铁颗粒,且在标记后4周内细胞仍具有活力,标记后的BMSCs在T2WI、尤其是GRE(T2*WI)序列信号明显降低;并且细胞浓度越高信号降低越明显,GRE序列MR成像的最低细胞浓度为5×104/ml。当标记细胞浓度为5×104/ml时,信号在T2*WI序列的降低2周后失去统计学意义;而在细胞浓度为5×105/ml时,标记3周后,信号在T2*WI序列的降低才失去统计学意义。结论:25μg/ml铁浓度标记干细胞不仅标记效率高,而且对细胞生长及增殖活力无明显影响,标记后MR信号改变与干细胞数目及标记时间相关。     

血小板膜GPⅡb/Ⅲa单抗及水蛭素对损伤动脉内膜血小板沉积影响的实验研究

本研究在单条动脉实验模型上,应用Ⅰ标记血小板观察了CD_(w41)及本蛭素对模拟血管成形术后血小板沉积量的影响.结果表明,仅用肝素抗凝组(2单位/ml)损伤段血小板沉积量为:9.42±2.01×10~6个血小板/cm~2和402.37±72.85×10~6个血小板/g;未损伤段血小板沉积量为:1.25±0.57×10~6个血小板/cm~2和67.53±18.46×10~6个血小板/g(P<0.01).CD_(w41)及水蛭素组损伤段血小板沉积量与对照组损伤段相比分别减少了73%和43%.     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

The role of interventional radiology in trauma

Introduction Interventional Radiology has evolved into a specialty having enormous input into the care of the traumatized patient.In all hospitals,regardless of size,the Interventional Radiologist must consider their relationships with the trauma service in order to     

人体中的镭-226、镭-228、钋-210、铅-210

本文报道了广东阳江高本底地区6名、对照地区8名人尸体的骨²²⁶Ra、²²⁶Ra的浓度以及部分居民内脏器官中。²¹⁰Po、²¹⁰Pb的浓度。结果轰明阳江高本底地区和对照地区居民骨镭-226、镭-228的浓度分别为29.9pCi/kg, 26.9pCi/kgl 8.7pCi/kg, 8.2pCi/kg.由此估算出阳江高本底地区屠民骨中²²⁶Ra、²²⁸Ra的负薄璧及对骨衬、骨髓所产生的剂量当量分别为对照地区民民的3.4倍, 3.3倍。两地区居民内脏器官中^{210Po、210Pb的测定分析铡数较少但仍看出, 高本底地区均明显高于对照地区.}     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).