Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress

The sea cucumber Apostichopus japonicus plays important roles in marine benthic ecosystem as environmental cleaners, and it is the important aquaculture species in China. High water temperature poses critical threat for the survival of A. japonicus, which has resulted in extensive death in summer. To explore the genes expression profiles under different levels of heat stress, the high-throughput RNA-seq was applied in this study. Our results revealed a total of 1 371, 1 225 and 1 408 differentia...     

基于中国北极考察布放浮标观测和数值模拟的海冰和积雪厚度变化分析

Sea ice and the snow pack on top of it were investigated using Chinese National Arctic Research Expedition(CHINARE) buoy data.Two polar hydrometeorological drifters,known as Zeno? ice stations,were deployed during CHINARE 2003.A new type of high-resolution Snow and Ice Mass Balance Arrays,known as SIMBA buoys,were deployed during CHINARE 2014.Data from those buoys were applied to investigate the thickness of sea ice and snow in the CHINARE domain.A simple approach was applied to estimate the average snow thickness on the basis of Zeno~ temperature data.Snow and ice thicknesses were also derived from vertical temperature profile data based on the SIMBA buoys.A one-dimensional snow and ice thermodynamic model(HIGHTSI) was applied to calculate the snow and ice thickness along the buoy drift trajectories.The model forcing was based on forecasts and analyses of the European Centre for Medium-Range Weather Forecasts(ECMWF).The Zeno~ buoys drifted in a confined area during 2003–2004.The snow thickness modelled applying HIGHTSI was consistent with results based on Zeno~ buoy data.The SIMBA buoys drifted from 81.1°N,157.4°W to 73.5°N,134.9°W in 15 months during2014–2015.The total ice thickness increased from an initial August 2014 value of 1.97 m to a maximum value of2.45 m before the onset of snow melt in May 2015;the last observation was approximately 1 m in late November2015.The ice thickness based on HIGHTSI agreed with SIMBA measurements,in particular when the seasonal variation of oceanic heat flux was taken into account,but the modelled snow thickness differed from the observed one.Sea ice thickness derived from SIMBA data was reasonably good in cold conditions,but challenges remain in both snow and ice thickness in summer.     

Analysis and comparison of heat flux of landfast ice during 2016 in the Prydz Bay,Antarctica

Long term in situ atmospheric observation of the landfast ice nearby Zhongshan Station in the Prydz Bay was performed from April to November 2016. The in situ observation, including the conventional meteorological elements and turbulent flux, enabled this study to evaluate the sea ice surface energy budget process. Using in situ observations, three different reanalysis datasets from the European Centre for Medium-Range Weather Forecasts Interim Re-analysis(ERA-Interim), National Centers for Environmental Prediction Reanalysis2(NCEP R2), and Japanese 55-year Reanalysis(JRA55), and the Los Alamos sea ice model, CICE, output for surface fluxes were evaluated. The observed sensible heat flux(SH) and net longwave radiation showed seasonal variation with increasing temperature. Air temperature rose from the middle of October as the solar elevation angle increased.The ice surface lost more energy by outgoing longwave radiation as temperature increased, while the shortwave radiation showed obvious increases from the middle of October. The oceanic heat flux demonstrated seasonal variation and decreased with time, where the average values were 21 W/m~2 and 11 W/m~2, before and after August,respectively. The comparisons with in situ observations show that, SH and LE(latent heat flux) of JRA55 dataset had the smallest bias and mean absolute error(MAE), and those of NCEP R2 data show the largest differences.The ERA-Interim dataset had the highest spatial resolution, but performance was modest with bias and MAE between JRA55 and NCEP R2 compare with in situ observation. The CICE results(SH and LE) were consistent with the observed data but did not demonstrate the amplitude of inner seasonal variation. The comparison revealed better shortwave and longwave radiation stimulation based on the ERA-Interim forcing in CICE than the radiation of ERA-Interim. The average sea ice temperature decreased in June and July and increased after September,which was similar to the temperature measured by buoys, with a bias and MAE of 0.9°C and 1.0°C, respectively.     

基于MODIS热红外数据的渤海海冰厚度反演

Level ice thickness distribution pattern in the Bohai Sea in the winter of 2009–2010 was investigated in this paper using MODIS night-time thermal infrared imagery.The cloud cover in the imagery was masked out manually.Level ice thickness was calculated using MODIS ice surface temperature and an ice surface heat balance equation.Weather forcing data was from the European Centre for Medium-Range Weather Forecasts(ECMWF) analyses.The retrieved ice thickness agreed reasonable well with in situ observations from two off-shore oil platforms.The overall bias and the root mean square error of the MODIS ice thickness are –1.4 cm and 3.9 cm,respectively.The MODIS results under cold conditions(air temperature –10°C) also agree with the estimated ice growth from Lebedev and Zubov models.The MODIS ice thickness is sensitive to the changes of the sea ice and air temperature,in particular when the sea ice is relatively thin.It is less sensitive to the wind speed.Our method is feasible for the Bohai Sea operational ice thickness analyses during cold freezing seasons.     

Western Indian Ocean SST signal and anomalous Antarctic sea-ice concentration variation

Teleconnection between El Nino/La Nina-Southern Oscillation (ENSO) phenomenon and anomalous Antarctic sea-ice variation has been studied extensively.In this study,impacts of sea surface temperature in the Indian Ocean on Antarctic sea-ice change were investigated during Janaury 1979 and October 2009.Based on previous research results,sea areas in the western Indian Ocean (WIO;50°–70°E,10 °–20 °S) are selected for the resreach.All variables showed 1-10 year interannual timescales by Fast Founer Tranaform (FFT) transformation.Results show that i) strong WIO signals emerged in the anomalous changes of Antarctic sea-ice concentration;ii) significant positive correlations occurred around the Antarctic Peninsula,Ross Sea and its northwest peripheral sea region iii) negative correlation occurred in the Indian Ocean section of the Southern Ocean,Amundsen Seas,and the sea area over northern Ross Sea;and iv) the atmospheric anomalies associated with the WIO including wind,meridional heat flux,and surface air temperature over southern high latitudes were the possible factors for the teleconnection.     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

北极夏季海冰单轴抗压强度研究

The results on the uniaxial compressive strength of Arctic summer sea ice are presented based on the samples collected during the fifth Chinese National Arctic Research Expedition in 2012(CHINARE-2012). Experimental studies were carried out at different testing temperatures(-3,-6 and-9°C), and vertical samples were loaded at stress rates ranging from 0.001 to 1 MPa/s. The temperature, density, and salinity of the ice were measured to calculate the total porosity of the ice. In order to study the effects of the total porosity and the density on the uniaxial compressive strength, the measured strengths for a narrow range of stress rates from 0.01 to 0.03 MPa/s were analyzed. The results show that the uniaxial compressive strength decreases linearly with increasing total porosity, and when the density was lower than 0.86 g/cm3, the uniaxial compressive strength increases in a power-law manner with density. The uniaxial compressive behavior of the Arctic summer sea ice is sensitive to the loading rate, and the peak uniaxial compressive strength is reached in the brittle-ductile transition range. The dependence of the strength on the temperature shows that the calculated average strength in the brittle-ductile transition range, which was considered as the peak uniaxial compressive strength, increases steadily in the temperature range from-3 to-9°C.     

转录组学分析B-3胞外多糖对巨噬细胞RAW264.7基因表达的影响

B-3胞外多糖是从一株南极嗜冷菌Psychrobacter sp.B-3发酵液中提取的胞外多糖,前期研究发现该多糖能够激活巨噬细胞并影响其免疫调控活动。为探明其免疫调控途径,采用转录组学分析对经B-3多糖刺激的RAW264.7巨噬细胞系进行了差异表达基因分析。研究结果表明B-3多糖的刺激引起了420个基因的差异表达,其中上调178个、下调242个。差异表达基因与细胞功能存在诸多关联,但主要集中在代谢和免疫两个通路上：其中在多个免疫相关通路上,富集最显著的是抗原处理和表达途径;另一个明显富集的则是代谢通路,B-3多糖处理引起了大量参与代谢的酶类的表达变化,包括氨基酸,碳水化合物,脂质和核苷酸代谢相关基因均发生了一定程度的改变。因此,B-3胞外多糖作为一种免疫激活剂直接影响了RAW264.7巨噬细胞系的免疫功能,同时也对其胞内的代谢途径产生了影响。本论文首次开展了南极嗜冷细菌胞外多糖对RAW264.7巨噬细胞系的影响,该研究为不同来源的活性多糖对巨噬细胞免疫激活研究提供了科学依据。     

Indices based on silicoflagellate assemblages offer potential for paleo-reconstructions of the main oceanographic zones of the Southern Ocean

This study reports detailed silicoflagellate assemblage composition and annual seasonal flux from sediment traps at four locations along a transect across the Southern Ocean frontal systems. The four traps sampled the central Subantarctic Zone (SAZ, 47°S site), the Subantarctic Front (SAF, 51°S site), the Polar Frontal Zone (54°S site) and the Antarctic Zone (61°S site) across the 140°E longitude. Annual silicoflagellate fluxes to the deep ocean exhibited a similar latitudinal trend to those of diatom fluxes reported in previous work, with maxima in the Antarctic Zone and minima in the Subantarctic Zone. The data suggest that, along with diatoms, silicoflagellates are important contributors to biogenic silica export at all sites, particularly in the Subantarctic Zone. Two main silicoflagellate genera were observed, with Stephanocha sp. (previously known as Distephanus) dominating polar waters and Dictyocha sp. important in sub-polar waters. This is consistent with previous use of the Dictyocha / Stephanocha ratio to infer paleotemperatures and monitor shifts in the position of the Polar Frontal Zone in the sedimentary record. It appears possible to further refine the application of this approach by using the ratio between two Dictyocha species, because Dictyocha aculeata dominated at the Subantarctic Front, while Dictyocha stapedia dominated in the central Subantarctic Front. Given the well-defined environmental affinities of both species, a new SAF silicoflagellate index (SAF-SI) based on this ratio is proposed as a useful diagnostic for SAF and SAZ water mass signatures in the Plio-Pleistocene and Holocene sedimentary record.     

偏顶蛤（Modiolus modiolus）转录组组装及其与深海偏顶蛤（Bathymodiolus platifrons）比较转录组研究

双壳类适应性进化遗传基础尚未有大量研究。而生活在沿海潮间带和深海环境中的双壳贝类适应性进化的分子机制更鲜有报道。我们首次测序组装了潮间带生活的偏顶蛤Modiolus modiolus的转录组序列。同时与生活在深海热液冷泉喷口的偏顶蛤Bathymodiolus platifrons进行了比较转录组分析,阐明了这两个物种在不同环境中的适应性进化机制。M. modiolus和B. platifrons的转录组组装共产生182,476和156,261个转录本,N50值分别为1,769和1,545。同时注释到27,868和23,588个基因。GO富集分析表明这些基因有相似的富集模式。两物种进化分析鉴定到10,245个直系同源基因,其中26个基因受到强烈正选择（Ka/Ks＞1）,12个基因受到中度正选择（0.5M. modiolus的转录组组装拼接序列,同时通过比较转录组学分析阐明了其与近缘物种B. platifrons的适应性进化机制。这为两个物种后续研究提供了序列资源和研究基础。     

南极冰藻Chlamydomonas sp. ICE-L过氧化氢酶的热胁迫响应

为了研究南极冰藻的热胁迫应答机制,本文根据转录组测序得到的冰藻Chlamydomonas sp. ICE-L过氧化氢酶CiCAT基因分析了其编码蛋白的特征,同时研究了CiCAT基因表达和过氧化氢酶活性在培养温度升高时的响应变化情况。结果表明:CiCAT基因序列全长为2 066 bp,编码492个氨基酸。在过氧化氢酶的系统进化树中,南极冰藻与其他绿藻聚类为一个分支。CiCAT编码的蛋白序列与盐藻和红球藻的过氧化氢酶序列相似性较高,分别为80.5%和78.9%。当南极冰藻处于热胁迫条件下,CiCAT基因的相对表达量和过氧化氢酶活均呈现出先上升后下降的趋势,但在胁迫24 h时CiCAT基因的表达量变化不明显,而实验组的酶活显著高于对照组。在胁迫72 h时,基因表达量和酶活均达到最高值。研究初步表明,与在常温藻类和高等植物中的功能相似,在经受热胁迫的情况下,南极冰藻中的抗氧化酶系统也发挥着重要作用。     

南海北部亚热带滨珊瑚对低温的耐受能力

Marginal scleractinian corals growing at their latitudinal limits should be quite sensitive to variations in winter sea surface temperatures(SSTs). An extreme cold event occurring in early 2008 offered a unique opportunity to examine the effect of cold-water anomalies on Porites lutea corals and their physiological tolerance and acclimation in the subtropical northern South China Sea(NSCS). Besides in-situ observation, a subsequent aquarium-based experiment was designed for reproducing the chilling process and a 50-year-long Sr/Ca ratio profile from two P. lutea skeletal slabs was analyzed for reconstructed the historical annual minimum SSTs which ceased Porites calcification. The 2008 low-temperature anomaly caused the minimum daily mean SSTs dropped below 13°C in the Daya Bay. The stress symptoms displayed by local P. lutea colonies included polyp retraction, reduced coloration and pale, but none showed tissue sloughing. The ability of P. lutea to survive implied its tolerance of extreme low temperatures. Here we suggest a model on the tolerance of high-latitude Porites under low-temperature stresses, which is when SSTs drop below 18°C, Porites corals contract their tentacles(losing heterotrophic capability), then cease calcification(reducing energy consumption), and meanwhile maintain relatively high levels of zooxanthellae density(sustaining host's life via photosynthetic capacity of symbiotic zooxanthellae). This study revealed remarkable acclimatization of P. lutea corals to low temperature extremes. This acclimatization is beneficial for Porites corals in the NSCS to expand their living ranges towards the higher-latitude areas and have the potential to be the incipient reef former.     

北极细菌Pseudoalteromonas sp.A2对H2O2胁迫的转录组分析

海水中的高溶解氧浓度、低温和UV辐射导致了氧胁迫是北极海洋细菌面临的主要胁迫因素之一。本文对北极细菌Pseudoalteromonas sp.A2对H₂O₂导致的氧胁迫的应答特征进行了转录组测序和基因差异表达的比较分析,以期发现与氧胁迫相关的关键功能基因。研究表明,与对照组相比,1 mmol/L的H₂O₂可导致菌株A2转录组的很大变化,包括109个基因的显著上调与174个基因的显著下调。COG分析表明,在功能已知的基因中,与转录后修饰、蛋白质转换和分子伴侣相关的基因大部分显著上调,而与氨基酸运输和代谢等相关基因则大部分显著下调。值得指出的是,有18个与鞭毛相关的基因和4个与热激蛋白相关的基因显著上调;同时,有9个与细胞色素和细胞色素氧化酶相关的基因和5个与TonB依赖受体相关的基因显著下调。在GO分析表明具有抗氧化活性的18个基因中,只有1个基因的表达显著上调,而有2个显著下调。简言之,RNA-Seq表明,除了传统的抗氧化基因和应激蛋白外,鞭毛相关基因和功能未知基因在菌株Pseudoalteromonas sp.A2的氧胁迫适应性中起着重要作用。该转录组分析和氧胁迫相关基因的发现有助于了解北极细菌的氧胁迫适应机制。     

温度、盐度和光照对浒苔生长和光合作用的影响

Intensive Pyropia aquaculture in the coast of southwestern Yellow Sea and its subsequent waste, including disposed Ulva prolifera, was speculated to be one of the major sources for the large-scale green tide proceeding in the Yellow Sea since 2007. It was, however, unclear how the detached U. prolifera responded and resumed growing after they detached from its original habitat. In this study, we investigated the growth and photosynthetic response of the detached U. prolifera to various temperature, salinity and irradiance in the laboratory. The photosynthetic rate of the detached U. prolifera was significantly higher at moderate temperature levels(14–27°C)and high salinity(26–32), with optimum at 23°C and 32. Both low(14°C) and highest temperature(40°C), as well as low salinity(8) had adverse effects on the photosynthesis. Compared with the other Ulva species, U. prolifera showed higher saturated irradiance and no significant photoinhibition at high irradiance, indicating the great tolerance of U. prolifera to the high irradiance. The dense branch and complex structure of floating mats could help protect the thalli and reduce photoinhibition in field. Furthermore, temperature exerted a stronger influence on the growth rate of the detached U. prolifera compared to salinity. Overall, the high growth rate of this detached U. prolifera(10.6%–16.7% d~(–1)) at a wide range of temperature(5–32°C) and salinity(14–32) implied its blooming tendency with fluctuated salinity and temperature during floating. The environmental parameters in the southwestern Yellow Sea at the beginning of green tide were coincident with the optimal conditions for the detached U. prolifera.     

刺参(Apostichopus japonicus)高温胁迫消减cDNA文库的构建与分析

采用抑制性消减杂交技术(SSH)研究刺参高温胁迫下差异表达的基因.分别以高温实验组为检测组(tester)、常温对照组为驱动组(driver),进行正向抑制性消减杂交;以常温组为tester、高温组为driver,进行反向消减杂交,成功构建了刺参正反双向差异消减文库.从两个文库随机挑选384个白斑克隆进行斑点杂交进一步...