宫颈解脲支原体感染与输卵管妊娠的关系探讨

目的探讨宫颈解脲支原体感染与输卵管妊娠的关系。方法对72例输卵管妊娠患者(观察组)和46例早孕妇女(对照组)进行宫颈分泌物解脲支原体培养,观察两组检测对象宫颈解脲支原体感染情况。结果输卵管妊娠患者宫颈分泌物解脲支原体阳性率为44.4%,对照组宫颈分泌物解脲支原体阳性率为21.7%,两组解脲支原体感染率间差异有统计学意义(P<0.01)。结论宫颈解脲支原体感染与输卵管妊娠有较密切的关系,防治解脲支原体是减低输卵管妊娠发病率和彻底治疗输卵管妊娠的重要措施之一。     

穿通支原体在宫颈癌患者血液和肿瘤组织中的分离检出

目的 探讨穿通支原体（Mycoplasma penetrans，Mpe）分离检出与宫颈癌发生的相关性。方法 将55例宫颈浸润癌、37例宫颈上皮内瘤变（CIN）及24例对照的宫颈组织、血液标本接种在改良的SP-4、Uu、Mh等液体培养基进行支原体分离培养，可疑阳性菌株用PCR予以证实。MpePCR阳性患者的组织和血液标本超薄切片．电镜下观察Mpe颗粒的存在以及细胞超微结构改变。结果 55例宫颈浸润癌患者中共分离到Mpe25例，分离率为45．5％（25／55），37例宫颈CIN患者中共分离到Mpe9例，分离率为24．3％（9／37），24例对照组中有1例从血液和组织同时分离到Mpe（4．2％，1／24），各组Mpe分离率比较均有统计学意义。电镜下发现，Mpe阳性的宫颈鳞状细胞癌患者的血液、组织中均有典型葫芦状、三层膜结构的穿通支原体存在。结论 宫颈浸润癌、宫颈CIN患者的组织、血液中Mpe分离阳性率高，提示Mpe感染与宫颈癌的发生有一定关联。     

沙眼衣原体和解脲支原体培养结果分析

目的　通过培养了解由沙眼衣原体 (CT)、解脲支原体 (Uu)引起非淋菌性尿道 (宫颈 )炎感染的现状和特点。方法　对 135 0例临床拟诊非淋菌性尿道 (宫颈 )炎患者的生殖道拭子 ,同时采用CT细胞培养及Uu液体培养。结果　总阳性率为 35 .0 % ,CT阳性率为 8.0 %。Uu为 2 0 .3% ,混合阳性率 6 .8%。男女性别间感染率差异有非常显著的统计学意义 (χ2 =6 0 .4 4 ,P <0 .0 1)。初诊患者 5 17例 ,其中阳性 2 4 5例 ,阳性率为 4 7.4 %。复诊患者833例 ,阳性 2 2 8例 ,阳性率为 2 7.4 %。两者差异有非常显著的统计学意义 (P <0 .0 1)。结论　广州市皮肤病防治所性病门诊拟诊非淋菌性尿道 (宫颈 )炎患者 135 0例中 ,检出阳性 4 73例 ,尤其混合性感染应引起重视。     

解脲脲原体的血清型聚类分析

近年来,非淋球菌性尿道(阴道)炎(NGU)发病人数迅速增加,其报告病例数在许多城市已经超过淋病,跃居8种法定报告性病的首位。在导致NGU的病原体中,人们对解脲脲原体(Ureaplasma urealyticum,Uu)的争议较大,这与Uu血清型众多、基因分型及检出方法不同有关。为了更好地阐明Uu的致病性,本文拟从不同人群、不同地区及不同疾病中Uu各型别的分布特征出发,利用统计学聚类分析法,探讨Uu各血清型的致病规律。1材料与方法1.1资料来源检索《中国期刊网》中有关Uu血清学分型研究报道,共23篇,根据资料的完整性和有效性,纳入12篇有效文献〔1-12〕,进行…     

支原体感染与新生儿疾病关系的研究

目的　研究支原体感染与新生儿疾病之间的关系。方法　采用nPCR对 15 6例新生儿患儿及 5 0例正常新生儿的咽拭子 ,进行解脲脲原体 (Uu)、人型支原体 (Mh)、生殖支原体 (Mg)和发酵支原体 (Mf)特异性核酸检测。 结果　 15 6例新生儿患儿中检出Mh ,Mu ,Mg和Mf的阳性率分别为 84 .6 % ,4 6 .7% ,1.92 % ,0 %。 (Uu +Mg)双重同时感染率为 4 0 .3%(6 3/ 15 6 ) ,(Uu +Mh +Mg)三重感染率为 1.92 % (3/ 15 6 )。 5 0例正常新生儿仅 1例检出Uu。结论　同新生儿疾病支原体感染显著相关。     

女性生殖道解脲脲原体感染状况及其分群价值的研究

目的研究女性生殖道解脲脲原体(Uu)的感染状况和分群。方法选择2009年8~12月拟诊为尿道炎、阴道炎的女性患者668例为病例组,同期体检女性396例为对照组,取宫颈管分泌物同时进行Uu液体培养和固体培养,阳性标本采用PCR技术进行分群,再随机挑选部分阳性标本进行DNA测序。结果病例组Uu阳性率39.67%(265/668),对照组Uu阳性率34.34%(136/396),差异无统计学意义(P0.05);病例组检出生物群206例(77.74%),对照组检出生物群101例(74.26%),差异无统计学意义(P0.05);病例组Uu感染者中检出生物1群146例(占70.87%),检出生物2群48例(占23.30%),混合感染12例(占5.83%),对照组Uu感染者中检出生物1群85例(占84.16%),检出生物2群10例(占9.90%),混合感染6例(占5.94%),差异有统计学意义(P0.05)。结论健康女性泌尿生殖道可存在无症状的Uu携带状态。由于反复感染,存在未定型的解脲脲原体。Uu生物1群可能为泌尿生殖道的正常寄生微生物,而Uu生物2群可能是致病群。     

膀胱移行上皮细胞癌中穿通支原体的检出及意义

目的探讨穿通支原体(Mycoplasma penetrans,Mpe)在膀胱移行上皮细胞癌患者血液和肿瘤组织中的分离检出及意义。方法将55例膀胱移行上皮细胞癌患者的膀胱组织标本、血液标本分别接种于改良SP-4液体培养基中进行支原体分离培养,可疑阳性菌株用PCR予以证实。结果55例患者的Mpe累计分离率为41.8%(23/55),对照组的分离率为11.1%(3/27),两组比较有统计学意义(P<0.05)。将患者按UICC-TNM标准进行分期,浸润癌(T2~T4)组Mpe分离率为69.0%(9/13),非浸润癌(Ta~T1)组为33.0%(14/42),两组比较差异也有统计学意义(P<0.05)。结论膀胱移行上皮细胞癌患者的膀胱组织、血液中Mpe分离阳性率高,且浸润性膀胱癌组较非浸润癌组高,提示Mpe感染与膀胱移行上皮细胞癌的发生有一定关联,与肿瘤是否浸润也存在一定关系。     

乌鲁木齐高考学生输血传播病毒感染的比较分析

目的 研究输血传播病毒(TTV)在新疆乌鲁木齐市体检高考学生和HBV携带考生中的感染情况,以探讨输血传播病毒的致病特性.方法 以公布的TTV第一读码区序列设计两对寡核苷酸引物,用套式PCR方法,对120例体检HBsAg阴性考生和46例HBV携带考生血清进行TTV- DNA的检测.结果 120例体检HBV阴性考生中检出4例TTV-DNA阳性血清,阳性率为3.3%;46例HBV携带考生中检出8例TTV-DNA阳性血清,TTV-DNA阳性率为17.4%,两组相比差异有统计学意义(χ2=7.81, P﹤0.01).结论 新疆考生中存在TTV的感染, HBV携带考生中TTV的感染率较高.     

贵阳市健康妇女UU和Mh携带率的流行病学调查

解脲支原体(UU)和人型支原体(Mh)是非淋菌性尿道炎(NGU)的主要病原体之一,同时它们也可存在于健康人群中。为了解贵阳市健康妇女UU和Mh的携带率,于2004年8月对贵州省水泥厂410名已婚女工进行健康体检,筛选出157名健康妇女进行宫颈分泌物UU和Mh培养检测。1对象与方法1·1调查对象157名女工均为已婚妇女,其性伴只有配偶,均无自觉症状。年龄25~67岁,平均42·94岁。汉族146人,少数民族11人。有孕产史者153人,占97·5%;放置宫内节育器者70人,占44·59%。所有女工均通过妇检、B超排除宫颈炎、盆腔炎等妇科炎症和肿瘤,通过白带常规检查排除阴…     

淋病患者AIDS相关支原体的分离培养与核酸检测

目的　探讨淋病患者泌尿生殖道是否存在AIDS相关支原体。方法　对 15 6例男女性淋病患者泌尿生殖道分泌物进行了生殖支原体 (Mycoplasmagenitalium ,Mg)、发酵支原体 (M fermentans,Mf)、穿通支原体 (M penetrans,Mpe)、梨支原体 (M pirum ,Mpi)等 4种支原体分离培养和套式聚合酶链反应 (nPCR)核酸检测。 结果　 15 6例患者中共分离出Mg7株(4 5 % )、Mf1株 (0 6 % )、Mpe2株 (1 3% ) ,nPCR检测结果与之相同。结论　我国淋病患者中存在AIDS相关支原体感染。     

重庆江北地区非淋菌性尿道炎病原体检测分析及药敏分析

目的通过分析我院2007年度收治的非淋菌性尿道炎患者衣原体和支原体感染及药敏情况,为临床提供最新的流行病学资料及合理用药的依据。方法采用衣原体快速检测,支原体培养、药敏一体化方法检测我院妇产科、皮肤科、泌尿外科送检的泌尿生殖道分泌物,分析就诊患者的性别、年龄、衣原体检测、支原体培养药敏试验结果等资料。结果2007年全院检测疑似非淋菌性尿道炎患者1446例,衣原体感染阳性率为10．93％,支原体感染阳性率为23．24％。支原体药敏结果表明,对多西环素、交沙霉素、克拉霉素比较敏感,对四环素、氧氟沙星耐药明显。结论支原体（尤其是解脲支原体）感染已成为非淋球菌性泌尿生殖系统炎症的主要病原体之一,临床治疗应根据药敏结果合理用药,以减少耐药菌株产生。     

Mycoplasma hominis and Ureaplasma urealyticum in patients with sterile pyuria

OBJECTIVES: Mycoplasma hominis and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. The aim of this study was to asses the prevalence of these organisms in patients with sterile pyuria. METHODS: From December 2000 to June 2001 all urine samples sent in to microbiological diagnosis, which yielded > or =500 leucocytes/ml and <10(4) bacteria/ml, were collected for this study. Thirty-three samples from 30 patients (female: 21, male: nine; median age: 60 years, range: 23-91 years) met these criteria and were subjected to PCR for detection of M. hominis and U. urealyticum, respectively. RESULTS: M. hominis and U. urealyticum were detected in 2 (7%) and 6 samples (20%), respectively. With regard to the underlying diseases of the patients, glomerulonephritis was significantly associated with the detection of urogenital mycoplasmas (p=0.02). CONCLUSION: The prevalence of M. hominis and U. urealyticum found in this study corresponds to the expected prevalence in the general population. This finding does not indicate an association of sterile pyuria with urogenital mycoplasma infection/colonization.     

非淋球菌性尿道炎中生殖支原体的检测

为了探讨生殖支原体（Ｍｇ）与非淋球菌性尿道炎（ＮＧＵ）的关系,我们建立了套式ＰＣＲ法对２１６例ＮＧＵ病人尿道（阴道）分泌物标本做了Ｍｇ检测。分别扩增得到２９６ｂｐ和３７１ｂｐ片段。套式ＰＣＲ扩增片段经Ｅ．ｃｏＲＩ酶切和与γ３２Ｐ－ＡＴＰ标记的ＭＧＳ－Ｉ探针做斑点杂交,均证明扩增片段为Ｍｇ－Ｐａ。此方法灵敏度为１０３ｃｆｕ／ｍｌ。用此引物对其它８种支原体扩增均为阴性,具有高度的特异性。实验结果表明,ＮＧＵ患者Ｍｇ－ＰａＤＮＡ总阳性率为７．８％（１７／２１６）正常人群阳性率１．２％（１／８１）,两组有显著性差异（ｘ２＝３．９８５,Ｐ＜０．００５）。从不同性别比较,男性ＮＧＵＭｇ－ＤＮＡ检测阳性率１０．２％（７／６８）,女性６．７％（１０／１４８）,男性略高于女性。成人与儿童比较,成人ＮＧＵＭｇ－ＤＮＡ检测阳性率１０．３％（１１／１０６）儿童检测阳性率５．４％（６／１１０）,成人略高于儿童,统计学亦无显著性差异。结果提示Ｍｇ是ＮＧＵ病原体之一,在正常人群中也检测出阳性,说明它有正确寄居的可能性。     

Mycoplasma genitalium in non-gonococcal urethritis--a study in Swedish male STD patients

Urethral swab specimens obtained from 101 men attending an STD clinic were examined for the presence of Mycoplasma genitalium by polymerase chain reaction (PCR) amplification. Fifty patients had non-gonococcal urethritis (NGU), and 51 patients were included as controls without urethritis. M. genitalium DNA was detected in 13 (26%) of the urethritis patients and in 5 (10%) of the control patients (P=0.06). No patient positive for M. genitalium had a simultaneous chlamydial infection. Thus, in the 36 patients with non-chlamydial NGU, the prevalence of M. genitalium infection was 36% (P=0.007 compared with controls). All patients with M. genitalium positive urethritis had a high grade urethritis defined as >10 polymorphonuclear cells per high power microscopical field. Compared with the control group, those with M. genitalium positive urethritis had more often had a history of urethritis than had those with chlamydial NGU or those with M. genitalium negative, non-chlamydial NGU.     

The association of Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma genitalium with bacterial vaginosis: observations on heterosexual women and their male partners

The prevalence of 3 mycoplasmas (Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma genitalium) was determined in a cohort of women with or without bacterial vaginosis (BV) and in their respective male partners. Heterosexual women with or without BV and their male partners were recruited and genital sampling for these microorganisms was performed. Seventeen women with BV and 21 women with normal flora, and their respective male partners, were recruited. M. hominis was present in 9 (53%) of 17 women with BV compared with none of 21 women without BV (P=0.0001). Of the 17 male partners of women with BV, 8 (47%) had M. hominis compared to 5 (24%) of 21 male partners of women without BV (not significant [n/s]). U. urealyticum was detected in 11 (65%) of 17 women with BV in comparison with 10 (48%) of 21 women without BV (n/s). U. urealyticum was present in 4 (24%) of 17 male partners of women with BV compared to 6 (29%) of 21 male partners of women without BV (n/s). M. genitalium was not detected in any of 15 women with BV and in only 2 (12%) of 17 women without BV (n/s). M. genitalium was present in 4 (25%) male partners of 16 women with BV in comparison with 3 (16%) male partners of 19 women without BV (n/s). Thus, M. hominis was the only mycoplasma detected significantly more often in women with, rather than in those without, BV. None of the mycoplasmas was found significantly more often in male partners of women with, rather than those without, BV. Overall, M. genitalium behaved somewhat similar to Chlamydia trachomatis. It was the least commonly occurring mycoplasma, a reflection perhaps of the relatively low incidence of partner change in this study population.     

Genital mycoplasmas in women attending a family planning clinic in Guiné-Bissau and their susceptibility to antimicrobial agents

A study on the prevalence of genital mycoplasmas and their susceptibility to the most common antimicrobial agents used for treating the infection was conducted on 94 women attending a family planning clinic in Guiné-Bissau. Fifty-four women (57.4%) were positive for Mycoplasma hominis and/or Ureaplasma urealyticum. M. hominis and U. urealyticum separately isolated from infected women yielded frequencies of 31.5 and 27.8%, respectively, the remainder were infected with both species. No strain was found to be resistant to all three commonly employed antibiotics for the management of these infections (erythromycin, tetracycline and ofloxacin), although multiple resistance to two antibiotics was frequent, especially when both genital mycoplasmas were present. Some 90.7 and 24.1% of all isolates were resistant to erythromycin and tetracycline, respectively. No resistance was observed to ofloxacin, although 50% of the strains had intermediate resistance. The high prevalence of genital mycoplasmas in women attending a family planning clinic in Guiné-Bissau, as demonstrated in this study, appears to be associated with trichomonosis and bacterial vaginosis. These infections were also found to be highly resistant to erythromycin and tetracycline and to have intermediate resistance to ofloxacin. However, further studies are necessary to establish the burden of infection due to antibiotic resistant genital mycoplasmas.     

成人咽部解脲支原体定植与性行为的关系

对１６３名性乱者咽部和尿生殖道标本和６３名健康成人咽部标本进行解脲支原体（Ｕｒｅａｐｉａｓｍａｕｒｅａｌｙｔｉｃｕｍ，Ｕｕ）检测，结果表明性乱人群咽部Ｕｕ定植率（４１．１％）极显著高于健康成人（６．３％），（Ｐ＜０．００１），咽部Ｕｕ定植与性活动、多性伴、性乱交和口交等不洁性行为以及Ｕｕ尿道炎有明显关系，性乱人群咽部Ｕｕ阳性者中，７３．１％的人有直接不洁性行为史，４４．７％的人有Ｕｕ尿生殖道感染，２９．６％和８５．２％的女性患者有间接不洁性行为史和阴道炎。但咽部Ｕｕ阳性者咽部自觉症状不明显，仅１０．４％的人有咽部充血或咽炎表现。提示Ｕｕ在成人咽部可能以共生形式定植，其定植率随性活动而增加，并可通过性活动而传播     

生殖支原体、解脲脲原体对12种抗生素的药物敏感性分析

目的提高非淋菌性尿道炎（NGU）临床合理用药率。方法对61株生殖支原体（Mg）、252株解脲脲原体（Uu）行12种抗生素的药物敏感性试验。结果 Mg对阿奇霉素、美满霉素、克拉霉素、强力霉素、罗红霉素、交沙霉素的敏感性较高。Uu对强力霉素、美满霉素、克拉霉素、交沙霉素、四环素、阿奇霉素的敏感性较高。Mg、Uu对喹诺酮类药物均不敏感。结论对NGU患者不宜采用喹诺酮类药物作为经验治疗;宜根据药物敏感性结果足量足疗程使用抗菌药物,无药物敏感试验参考时,可使用美满霉素、克拉霉素、强力霉素、交沙霉素、阿奇霉素治疗。     

Chlamydia trachomatis in the pharynx and rectum of heterosexual patients at risk for genital infection

Although urogenital infections with Chlamydia trachomatis are well recognized, less is known about infection at other body sites in adults. Pharyngeal specimens obtained from 706 heterosexual men and 686 women, and rectal specimens obtained from 1223 women who were at risk for chlamydia infection were cultured for C. trachomatis. Urogenital specimens were obtained from all patients. Chlamydia trachomatis was isolated from the pharynx in 3.7% of men and 3.2% of women. Recovery of chlamydiae was not associated with the presence of pharyngeal symptoms, but in women, but not men, it was associated with a history of oral-genital sex. The organism was also recovered from the rectum of 5.2% of the women. Rectal isolation did not correlate with a history of rectal symptoms or rectal sex but did correlate with concurrent genital infection. Infection at these sites may be important in the transmission or persistence of C. trachomatis infections.