牛和猪人工感染牛带绦虫的囊尾蚴形态与肝组织的病理变化

将试验动物牛和猪分为4组：A组(从江牛带绦虫感染牛组)、B组(从江牛带绦虫感染猪组)、C组(都匀亚洲牛带绦虫感染牛组)及D组(都匀亚洲牛带绦虫感染猪组)。分别灌喂从江牛带绦虫和都匀亚洲牛带绦虫的孕节，并于感染后第67d剖检，对牛带绦虫人工感染牛、猪的囊尾蚴形态与肝组织的病理变化进行了观察比较。结果显示，A组、B组、C组囊尾蚴分布在肝，C组囊尾蚴分布在肝、心、肾及四肢肌肉等；A组、B组囊尾蚴外有少量纤维组织包绕，A组囊尾蚴囊壁向囊腔内突出生长，周围有少量嗜酸性粒细胞浸润；B组、D组囊尾蚴发生坏死、钙化，周围有大量嗜酸性粒细胞及中性粒细胞浸润，D组肉芽肿形成，B组、D组均有大量纤维组织增生。     

山羊细颈囊尾蚴病例的诊疗

细颈囊尾蚴是由寄生于犬的小肠内的泡状带绦虫的幼虫感染引起的,寄生于易感动物的肝脏、胃网膜和肠系膜等处的一种寄生虫病,主要侵害2-12月龄的绵羊和猪,山羊、牛、鹿等也可感染。绵羊细颈囊尾蚴的感染率25%,以牧区感染较重,但死亡率不高。9月5日,笔者诊治了一起由细颈囊尾蚴引起的山羊失明等症状病例。现报告如下：     

牛带绦虫囊尾蚴头节翻出的实验观察

目的 观察在不同浓度猪胆汁、生理盐水中牛带绦虫囊尾蚴头节翻出情况。方法 用牛带绦虫虫卵感染乳牛66d后剖检收集的囊尾蚴，分别放入不同浓度的胆汁、生理盐水中，37℃恒温箱培养，计算头节翻出率。结果 在100％和50％胆汁中于10min和20min头节翻出率为100％，而在浓度为25％、12％、6％胆汁、生理盐水中30min，头节翻出率分别是100％、70％、50％和10％。结论 在不同浓度胆汁、生理盐水中头节翻出率各不相同，最快1min，且随时间增加，头节翻出率越高。     

牛带绦虫病和牛囊尾蚴病的防治

正牛带绦虫病为寄生在人体小肠当中的人畜共患绦虫病,在世界各地均有分布,其中在东非、中东以及近东等国家、拉美等国家比较流行。在我国的广西、云南和贵州等地比较流行。其中绦期的幼虫-牛囊尾蚴能够在牛的肌肉中寄生,如果人吃了生的或半生不熟的感染牛肉,便会被感染。1牛带绦虫病的防治1.1感染症状牛带绦虫病为由牛带绦虫成虫寄生人体小肠引起的一种肠绦虫病,又称牛肉绦虫病、肥胖带绦虫病。该病的感染不     

两种带绦虫感染家猪的病理学比较研究

为了探明亚洲带绦虫和牛带绦虫在家猪体内的发育规律及致病性,试验用两种带绦虫卵分别灌胃感染家猪,于不同时间剖检,观察两种带绦虫在家猪体内的发育情况,并取肝组织进行病理检查.结果表明:家猪对两种带绦虫卵均易感,是亚洲带绦虫的适宜中间宿主,牛带绦虫的非适宜中间宿主;亚洲带绦虫囊尾蚴寄生数量多,各期发育良好;而牛带绦虫囊尾蚴寄...     

猪囊尾蚴基质金属蛋白酶-9在猪肌肉和脑组织的表达分布

应用免疫组化分析猪囊尾蚴基质金属蛋白酶-9(MMP-9)的表达.用猪带绦虫卵1 mL(8万个/mL)灌胃20 d龄健康乳猪6头,于感染后40、80、120 d分别宰杀2头猪并取含囊尾蚴的肌肉及脑组织制作4μm厚石蜡切片,采用Envision二步法,观察不同时期寄生在肌肉及脑组织的猪囊尾蚴基质金属蛋白酶-9的表达.结果显...     

牦牛囊尾蚴病一例

牛囊尾蚴是寄生在人体的无钩绦虫的蚴虫,多寄生于牛的咬肌、舌肌、心肌、肩胛肌、颈肌及臀肌等处,眼观形态与猪囊虫相似,但在镜检时可见到头节上仅有4个吸盘,而无顶突和小钩,一般在牛胴体中寄生的牛囊尾蚴数量很少,且多在深层肌肉中。笔者遇到一例牦牛囊尾蚴病一例,而该病在本地区屠宰牦牛胴体中很少见,现介绍如下。     

一例猪囊尾蚴病的诊治报告

<正>猪囊尾蚴病是一种世界性分布的人畜共患病寄生虫病。猪囊尾病是由猪带绦虫,链状带绦虫的中绦期幼虫猪囊尾蚴寄生于人、猪、犬、猫的肌肉、成虫寄生在人的小肠而引起的。感染者是主要传染源,通过排出的孕节和虫卵感染易感对象。本病的易感者是人和猪,包括野猪,人对猪囊尾蚴普遍易感。人是终末宿主,自然条件下是猪带绦虫的唯     

屠宰猪细颈囊尾蚴病的调查与思考

猪细颈囊尾蚴病是由带科泡状带绦虫(Taenia hydatigena)的幼虫阶段——细颈囊尾蚴(Cysticercus tenuicollis)所引起的。幼虫虫体俗称“水铃铛”，呈囊泡状，大小如黄豆至鸡蛋大不等，囊壁乳白色，囊内含透明液体和一个乳白色头节。虫体寄生于猪的肝脏浆膜、网膜及肠系膜等处，除影响猪的生     

北京桑普科澳饲料有限公司

细颈囊尾蚴病的病原体是泡状带绦虫的幼虫阶段——细颈囊尾蚴所引起的。成虫寄生在犬、狼等肉食兽的小肠，幼虫寄生在猪、牛、羊等多种动物的肝脏浆膜、网膜和肠系膜等处，也可寄生在肺部。     

Bovine cysticercosis in cattle slaughtered at Awassa municipal abattoir, Ethiopia: prevalence, cyst viability, distribution and its public health implication

A cross-sectional study was conducted from October 2005 to April 2006 on bovine cysticercosis in cattle slaughtered at Awassa municipal abattoir with the objective of determining the prevalence of Taenia saginata cysticercosis, cyst viability, distribution and its public health implication. Questionnaire survey involving 120 respondents was also conducted on human taeniasis. A total of 400 carcasses were examined during the study period, of which 105 (26.25%) were infected with T. saginata metacestodes. From a total of 3200 samples inspected, 500 cysticerci were detected in 141 samples, of which 221 (44.2%) were alive. The anatomical distribution of cysticerci were 65 (29.2%) heart, 56 (25.3%) shoulder muscle, 59 (26.7%) masseter, 23 (10.4%) tongue, 12 (5.4%) diaphragm, three (1.4%) liver, two (0.9%) lung and one (0.5%) kidney samples. The prevalence varied significantly between local and crossbred animals (OR = 3.15, P < 0.05), but not varied between sex, age groups and origin of the animals. T. saginata taeniasis was a widespread public health problem in the town with an overall prevalence of 64.2% (77 of 120). The potential risk factors for disease contraction were raw meat consumption, religion and occupational risks. In conclusion, the study revealed high prevalence of T. saginata metacestodes throughout the edible organs together with existence of deep-rooted tradition of raw meat consumption. This may magnify the public health hazards of T. saginata in the study area. As a result, the disease deserves due attention to safeguard the public health and further promote beef industry in the country.     

Prevalence of Cysticercus bovis in Australian cattle

Objective The first national abattoir survey of Cysticercus bovis (‘beef measles’) in cattle was conducted in February 2008. Methods During the data collection period, 493,316 cattle were subjected to standard postmortem procedures, including incision of the masseter and heart muscles. On-site veterinarians were asked to record the location of any C. bovis cysts, as well as the National Livestock Identification System ear tag numbers of infected animals. Veterinarians were asked to submit samples for laboratory confirmation by histology and polymerase chain reaction testing. Results Of the 23 samples submitted, none was positive for C. bovis by either diagnostic method. Conclusions Occasional, isolated diagnoses of beef measles are still made in most states of Australia, but since the last regional surveys were conducted 30 years ago, when the estimated prevalence was 50 to 200 per 100,000 cattle slaughtered, the parasite has become extremely rare.     

Bovine cysticercosis in Denmark. A study of possible causes of infection in farms with heavily infected animals

Epidemiological studies were made on 14 farms from which, during a 2 year period, 38 cattle had been condemned at slaughter, due to massive infections with Cysticercus bovis. By on-site investigations and interviews, attempts were made to identify the transmission routes of Taenia saginata eggs from human faeces into the environment and further on to cattle. The most frequent sources of infection were found to be sludge from septic tanks illegally applied on pasture or crops, in some cases after having been mixed with animal slurry. Animals in permanently housed herds were infected through the fodder or by contamination of the indoor environment by such slurry containing Taenia eggs. Other herds were infected by grazing pastures in close proximity to municipal sewage treatment plants. In contrast to earlier Danish observations, application on farmland of sewage sludge from municipal treatment plants was not involved in any of the reported outbreaks. This apparent change coincides with the implementation of more restrictive legislation for the agricultural use of sewage sludge in Denmark.     

Infection of normal C3H/HeN mice with Taenia saginata asiatica oncospheres

Normal C3H/HeN female mice were used to develop an animal model of Taenia saginata asiatica oncosphere infection. The host cellular immune response in this model was analyzed by a cytokine enzyme-linked immunosorbent assay (cytokine ELISA) and flow cytometry. Tumor-like cysts containing cysticerci were recovered from the inoculation sites of female mice 7 weeks postinfection with the T. saginata asiatica oncospheres. A sharp increase and sustained elevation in the ability of spleen cells to produce interferon-γ and interleukin (IL)-2 revealed that cellular immunity played an important role during the infection. An immediate increase in the levels of IL-6 at 1 week postinfection indicated the induction of a local acute inflammatory response. However, no significant change in the levels of IL-10 indicated that Th2 cells were not involved in this immune response. The patterns of cell distribution revealed by flow cytometry also supported the same finding. These results suggested that Th1 cells played a major role in the immune response in C3H/HeN mice during the early stages of the oncosphere infection and that the Th2 response was not induced during the stage of cysticercus formation.     

Taenia coenurus in the orbit of a chinchilla

A 4-year old, male intact, captive-bred chinchilla (Chinchilla lanigera) was presented due to progressive exophthalmos of the right eye over a 5-month period. Ophthalmic examination revealed exophthalmos with dorsal displacement of the right globe. Retropulsion was decreased and a fluctuant, subcutaneous mass could be palpated posterior and dorsal to the central aspect of the zygomatic bone. Transdermal ultrasonography revealed a fluid-filled mass consistent with a cyst located within the ventral right orbit. Computed tomography demonstrated dorsal displacement of the globe, lateral displacement of the zygomatic arch, and numerous mineral-dense foci within the lumen of the cyst. The cyst was removed en bloc by ventral transpalpebral orbitotomy. Histopathology revealed a single capsulated cyst with multiple invaginated protoscolices, characterized by a prominent scolex with refractile hooklets, suckers, and abundant calcareous corpuscles consistent with a Taenia coenurus. Exophthalmos resolved with surgical therapy and there was no evidence of recurrence or postoperative complications over a period of 2 years. To the authors' knowledge, this is the first reported case of an orbital cyst of parasitic origin in a chinchilla.     

Taenia crassiceps Infection in the Woodchuck: A Retrospective Study of 13 Cases

Abstract— Large, fluctuant, fluid-filled subcutaneous masses containing hundreds of Taenia crassiceps cysticerci were detected in 13 woodchucks trapped in central New York and Maryland. The axillary region (7 of 13 animals) and adjacent lateral thoracic wall (4 of 13 animals) were most frequently affected. One animal had a focal parasitic granuloma in the lung and another had numerous cysticerci present within the hepatic parenchyma. Histologically, parasite sections were surrounded by varying degrees of fibrosis and mild scattered to coalescing lymphocytic aggregates were present in the adjacent subcutaneous tissues. There was minimal infiltration of subcutaneous lesions into the underlying musculature. Scanning electron microscopy of the parasites indicated the presence of numerous developing buds in the area of the posterior bladder. Scanning and transmission electron microscopy indicated that cysticerci were covered with a dense coating of microtriches. Taenia crassiceps lesions were found in 13 (2.6 per cent) of 501 trapped woodchucks, and in none of 293 colony-born woodchucks. No relationship between Taenia crassiceps infection and woodchuck hepatitis virus infection could be established. Résumé— Des masses sous-cutanées volumineuses, fluctuantes, remplies de liquides, contenant des centaines de cysticerques de Taenia crassiceps furent découvertes chez 13 marmottes capturées au centre de l'Etat de New-York et dans le Maryland. La région axillaire (7 des 13 animaux) et la paroi thoracique latérale adjacente (4 des 13 animaux) étaient les plus fréquemment atteintes. Un animal avait un granulome parasitaire focal dans un poumon et un autre avaient de nombreux cysticerques dans le parenchyme hépatique. D'un point de vue histologique, les sections de parasite étaient entourées par une fibrose d'importance variable, et des aggrégats lymphocytaires modérés, dispersés ou coalescents, étaient présents dans les tissus sous-cutanés adjacents. Il y avait une infiltration minimale des lésions sous-cutanées dans les muscles sous-jacents. La microscopie électronique à balayage montra la présence de nombreux bourgeons en développement dans la zone postérieure de la vessie. La microscopie électronique à balayage et par transmission indiqua que les cysticerques étaient couverts par un enduit dense de microtriches. Des lésions dûes àTanenia crassiceps furent découvertes chez 2.6% (13 sur 501) des marmottes capturées, et 0% (0 sur 293) des marmottes nées en captivité. Aucune relation entre l'infestation par Taenia crassiceps et l'hépatite infectieuse virale de la marmotte ne put être établie. Zusammenfassung— Bei 13 im Zentrum von New York und Maryland gefangenen Waldmurmeltieren wurden große, fluktuierende, flüssigkeits-gefüllte subcutane Massen festgestellt, die Hunderte von Taenia crossiceps cysticerci enthielten. Am häufigsten waren der Axillarbereich (boi 7 von 13 Tieren) und die angrenzende seitliche Brustwand (bei 4 von 13 Tieren) betroffen. Ein Murmeltier hatte ein fokales parasitäres Granulom in der Lunge, ein anderes zahlreiche Cysticerci im Leberparenchym. Im histologischen Bild zeigten sich von verschieden starken Fibrosen umgebene Parasitenausschnitte und leicht verstreute bis konfluierende Lymphozytenaggregate im angrenzenden subcutanen Gewebe. Die darunterliegende Muskulatur wurde nur minimal von den subcutanen Läsionen infiltriert. Unter dem Rasterelektronenmikroskop zeigten die Parasiten zahlreiche sich entwickelnde Knospen im Bereich der hinteren Blase. Im Rasterelektronenmikroskop und im Transmissions-Elektronenmikroskop waren die Cysticeri mit einer dichten Schicht aus Mikrotrichen bedeckt. Taenia crassiceps-Läsionen wurden bei 2.6% (13 von 501) der gefangenen und bei 0% (0 von 293) der koloniegeborenen Waldmurmeltiere nachgewiesen. Ein Zusammenhang zwischen der Taenia crassiceps - Infektion und einer Waldmurmeltier-Hepatitis-Virusinfektion konnte nicht festgestellt werden. Resumen En 13 marmotas capturados en Nueva York y en Maryland se detectaron masas subcutáneas de gran tamaño, llenas de líquido y que contenían cientos de cisticercos de Taenia crassiceps. Las zonas más afectadas fueron la región axilar (7 de 13 animales) y la pared torácica adyacente (4 de 13 animales). Un animal presentaba un granuloma parasitario focal en un pulmón y otro presentaba numerosos cisticercos en el parénquima hepático. Histológicamente, los parásitos aparecían rodeados por una reacción fibrosa de diferente intensidad y en el tejido subcutáneo próximo se observaban agregados linfocitarios. La musculatura subyacente no aparecía afectada. Estudios de microscopía electrónica de transmisión y de barrido demostraron que los cisticercos estaben recubiertos por una densa capa de microtricos. Lesiones producidas par Taenia crassiceps se detectaron en el 2,6% (13 de 501) de los capturados y en el 0% (0 de 293) de los procedentes de un criadero. No se pudo demostrar relación alguna entre la infestación con Taenia crassiceps y la infección con el virus de la hepatitis de las marmotas.     

泡状带绦虫线粒体部分基因克隆及序列比对分析

采集甘肃兰州市区宠物犬体内两株泡状带绦虫,运用分子生物学方法对其线粒体部分基因进行克隆,并使用基因分析软件Larsergene V7.1进行序列分析,结果发现这两株虫体同源性达到99.6%,与国内的泡状带绦虫广东虫株和甘肃虫株同源性分别达到98.7%和98.4%,鉴定为泡状带绦虫;与其他带科绦虫同源性均高于85%,低于88%。其中,与国内牛带绦虫同源性最高,为88%;与国内巨颈带绦虫同源性最低,只有85.1%。推断不同带科绦虫的cox1基因种间差异明显,在带科绦虫的分类上意义较大。