补益中药复方对青少年哮喘患者缓解期IgE、IL-5Rα、HLA-A的影响

目的 研究复方中药敏康片治疗青少年哮喘的疗效和作用机理.方法 选择20例青少年哮喘患者进行治疗,19例青少年为正常对照组.哮喘组疗程为6个月,观察血清总IgE及白介素-5受体α链基因(IL-5Rα)、人类白细胞抗原(HLA-A)基因表达改变情况.结果 哮喘组IgE治疗前后有显著差异(P<0.01).IL-5Rα基因吸光度值治疗前较正常组高,治疗后降低.HLA-A基因吸光度值治疗前较正常组高,治疗后降低.结论 补益中药可以调整青少年哮喘患者相关基因表达,改善患者过敏状态,缓解临床症状.     

扶正治标法对青少年花粉症患者相关基因表达的影响

目的:研究中药治疗花粉症的作用机理。方法:选择50例花粉症患者,口服敏康片,治疗6个月后观察疗效及FcεRIβ,IL-4Rα,IL-5Rα,HLA-A基因表达情况,并以20例变应性皮炎和19例正常人为对照,用Northern blot检测FcεRIβ,IL-4Rα,IL-5Rα的变化。用RT-PCR法检测HLA-A,计算相对光密度值。结果:花粉症组患者症状有明显改善(P<0.05),治疗前后血清总IgE比较差异有统计学意义(P<0.01),花粉症组4个基因的表达治疗前与正常人比较,差异均具有统计学意义(P<0.01),治疗后与治疗前比较差异有统计学意义(P<0.01)。变应性皮炎组治疗前FcεRIβ,IL-4Rα与正常组比较差异有统计学意义(P<0.05),治疗前后FcεRIβ比较,差异有统计学意义(P<0.05)。鼻炎组、皮炎组HLA-A相对光密度值均较正常组高,两组治疗前后也有不同程度的改善(P<0.05)。结论:中药可以调整花粉症患者相关基因表达,改善患者过敏体质,缓解临床症状。     

介导过敏反应的自身抗体在自身免疫性疾病中的临床意义

自身免疫性荨麻疹的发病与机体产生针对IgE高亲和力受体α链（FcCRIα）或IgE的自身抗体、补体等免疫分子有关,同时抗FcεRIα自身抗体可能与内源性哮喘、伴有过敏症状的其他疾病密切相关。该抗体的检测将有助于相关疾病的深入研究与诊断治疗     

白细胞介素4受体基因多态性与支气管哮喘的相关性(摘要)

目的：探讨白细胞介素4受体（IL-4R）基因多态性与儿童支气管哮喘的相关性。方法：用聚合酶链反应／限制性片段长度多态性分析方法（PCR/RFLP）检测30例哮喘儿童和30例正常对照儿童IL-4R Q576R的多态性，并用酶联免疫吸附法（ELISA）检测两组儿童血浆总IgE的水平，然后进行χ^2检验及成组t检验。结果：（1）IL-4R杂合突变基因型Q576R、突变等位基因R576的分布频率在哮喘儿童及正常对照儿童中差异有显著性，     

IgE低亲和力受体基因mRNA表达水平与哮喘的关系

目的 探讨IgE低亲和力受c体(FcεRⅡ,CD23)基因表达水平在哮喘组与正常组间有无差异及基因表达与TlgE水平有无相关.方法 逆转录-聚合酶链反应(RT-Pc)检测FcεRⅡ基因表达水平,并用t检验作组间比较;直线相关分析基因表达与TlgE水平的相关性.结果 哮喘组TIgE水平与正常对照组之间差异有显著性(t=5.15,P＜0.01);FcεRⅡ基因表达水平在哮喘组与正常组之间差异有显著性(t=4.20,P＜0.01);在哮喘组与正常对照组中,FcεRⅡ基因表达水平均与TIgE水平间呈正的直线相关.结论 FcεRⅡ基因对血浆TIgE水平有调节作用,其功能变化在哮喘发病中可能有重要作用.     

哮喘患儿血清TNF-α、IL-8、IgE的动态变化及其临床意义

目的探讨哮喘儿童血清TNF-α、IL-8、IgE的动态变化及其临床意义。方法哮喘患者急性发作组88例及缓解期组78例、正常对照组70例,分别抽取其静脉血测定血清TNF-α、IL-8、IgE,IL-8、IgE应用双抗体夹心ELISA法检测,TNF-α应用ELISA法检测。结果哮喘急性发作组及缓解期组血清IgE、IL-8、TNF-α水平均明显高于对照组（P〈0．01）;哮喘急性发作期组血清IgE、IL-8、TNF-α水平也均明显高于缓解期组（P〈0．01）。结论IL-8可能参与了哮喘急性发作患儿的发病过程,可能发挥正反馈调节作用,促进哮喘患儿IgE分泌;哮喘缓解期仍存在炎症反应。TNF-α在启动哮喘气道炎症并使其持续存在中起关键作用。     

哮喘患者IL-4R基因多态性与sIL-4R、TIgE水平相关性研究

目的：探讨白细胞介素4受体（IL-4R）Ile50 Val多态性与哮喘患者血浆可溶性白细胞介素4受体（sIL-4R）、血浆IgE（IgE）水平及其相关性.方法：用等位基因特异性聚合酶链反应（Allele-speccifc PCR）检测哮喘与对照组位点多态性,用酶联免疫分析法（ELSIA）测定哮喘与对照组血浆IgE、可溶性白细胞介素4受体（sIL-4R）水平.结果：IL-4Rα链Ile50Val位点等位基因在哮喘组及对照组间分布的差异有显著性（P＜0.01）,等位基因Ile携带者其哮喘患病危险性较非携带者高2.64倍（P＜0.01）,三种基因型的分布在两组间的分布有显著性差异（P＜0.01）.在同一组内,Ile/Ile基因型血浆IgE水平高于Ile/Val及Val/Val基因型（P均＜0.01）,对血浆sIL-4R的影响相反.Ile/Val基因型血浆IgE、sIL-4R水平与Val/Val基因型之间无显著差异（P＞0.05）.结论：IL-4Rα链Ile50Val基因多态性是影响哮喘的重要候选基因.     

咳嗽变异型哮喘患者脱敏治疗前后γ干扰素和IL-4的水平

对30例咳嗽变异型哮喘患者脱敏治疗前和治疗1年后检测血清中γ干扰素（IFN-γ）、白细胞介素4（IL-4）以及总IgE的水平，并与30例对照者进行比较。结果显示脱敏治疗1年后，血清IFN-γ水平明显增高，IL-4和总IgE水平明显降低，治疗前后差异有统计学意义。提示脱敏治疗可调节咳嗽变异型哮喘患者血清IFN-γ和IL-4水平，降低总IgE，有效控制临床症状。     

哮喘患儿血清IL-4、TNF-α与IgE水平的探讨

目的 探讨IgE、IL-4与TNF-α在儿童哮喘发病中的作用以及IgE与IL-4的相关性。方法 采用酶联免疫吸附试验检测哮喘发作期40例、缓解期30例患儿及健康对照30例儿童IL-4、TNF-α水平和IgE水平。结果 哮喘组的IgE、IL－4与TNF-α水平均较对照组高，且发作期与缓解期、缓解期与对照组比较均有极显著性差异（P〈0．01）。IgE与IL-4呈正相关。结论 IgE、IL-4和TNF-α在儿童哮喘发病中起着重要作用，而且与哮喘的病情有一定的关系。     

细胞因子和IgE检测在支气管哮喘诊断中的价值

目的探讨支气管哮喘患者血清白细胞介素一6（IL-6）、白细胞介素一10（IL-10）、白细胞介素-16（IL-16）和免疫球蛋白IgE的动态变化和检测的临床意义。方法收集30例支气管哮喘发作期患者（哮喘组）和30例正常对照者（对照组）的血清,采用酶联免疫吸附试验（ELISA）测定血清中IL-6、IL-10、IL-16和总IgE。结果与正常对照组比较,哮喘组血清IL-6、IL-16和总IgE水平明显增高,相比较有显著性差异（P〈0．05）;而IL-10水平明显降低,相比较有显著性差异（P〈0．05）;哮喘组总IgE与IL-6、IL-16之间呈正相关（r=0．6871,r=0．8633,P〈0．01）;与IL-10之间存在负相关（r=-0．4782,P〈0．01）。结论IL-6、IL-10、IL-16和总IgE与哮喘发病机制有关,动态测定这些指标有助于病情评估。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.