新疆藁本有效成分研究

从新疆藁本(ConiselinumvaginatumThell)的根茎中分到七个化合物,根据理化常数和光谱解析,分别鉴定为coniselin(Ⅰ),(E)-3-methoxy-4,5-methyenedioxycinnamicaldehyde(Ⅱ),(E)-3-methoxy-4,5-methylenedioxycinnamicalcohol(Ⅲ),(E)-3-methoxy-4,5-methylenedoxycinnamicacid(Ⅳ),肉豆劳动蔻酸(myristicicacid,V),阿魏酸(ferulicacid,Ⅵ)和香草醛(vanilin,Ⅶ)。其中Ⅰ为新化合物,其余均为首次从该植物中分到。经药理实验表明化合物Ⅱ～Ⅴ对四氯化碳引起的小鼠转氨酶升高有降低作用,对丙酸杆菌引起的小鼠免疫性肝损伤有保护作用。     

GC法测定制剂中的咖啡因、醋氨酚和异丙安替比林

咖啡因(Ⅰ)、醋氨酚(Ⅱ)和异丙安替比林(Ⅲ)是退热剂的成分,用 GC 法对含有上述药的混合物进行了分离和测定,二乙氨乙茶碱(Ⅳ)为内标。(Ⅰ),(Ⅱ)和(Ⅲ)的保留时间分别为3.06,2.38和4.46min。其校正曲线呈直线的浓度范围分别为:(Ⅰ)0.77～2.15×10~(-3)mol/L,(Ⅱ)2.64～10.57×10~(-3)mol/L,(Ⅲ)1.73～7.78×10~(-3)mol/L。试验证明可同时测定混合物中的(Ⅰ),(Ⅱ)和(Ⅲ)。标准溶液用甲醇分别配制成如下标准溶液:(Ⅰ)1.56 mmol/L,(Ⅱ)7.89mmol/L,(Ⅲ)5.28 mmol/L 和(Ⅳ)6.33mmol/L。     

肝炎新药——联苯双酯的气液色谱分析

α-联苯双酯[4,4′-二甲氧基-5,6,5′,6′-二次甲二氧基-2,2′-二甲氧羰基联苯(Ⅱ)]系我所合成的新药之一,有明显的降谷丙转氨酶作用,对慢性迁延性和慢性活动性肝炎有显著疗效。有关该药的质量规格已制订。本品(Ⅱ)系用纯溴化物(Ⅰ-a)进行Ullmann反应制得,如果用溴化物粗品(其中除Ⅰ-a外,尚有Ⅰ-b等)则除(Ⅱ)外,将得(Ⅲ)和(Ⅳ)等同分异     

乌司他丁对大鼠实验性溃疡性结肠炎的作用

目的 探讨乌司他丁对大鼠实验性溃疡性结肠炎(UC)的作用及机制.方法 120只SD大鼠随机分为4组,每组各30只.使用三硝基苯磺酸钠( TNBS)建立UC模型,Ⅰ组不使用乌司他丁,为造膜对照组;Ⅱ、Ⅲ、Ⅳ组分别于造模0、6、12 h使用乌司他丁,剂量为2万单位/kg,为研究组.检测分析各组肠静脉内毒素、肿瘤坏死因子α(TNF-α)和UC模型症状积分(实验开始24、72、168 h各观察10只大鼠).结果 ①在实验各时段,使用乌司他丁组UC大鼠病变积分均低于Ⅰ组(均P＜0.05),且24、72 h时Ⅱ、Ⅲ、Ⅳ组差异均有统计学意义[24 h:(1.8±0.4),(2.7±0.3),(3.0±0.1);72 h:(3.7±2.3),(4.2±1.2),(5.0±0.9),均P＜0.05],但168 h时Ⅱ、Ⅲ、Ⅳ组未见明显差异.②使用乌司他丁组肠静脉内毒素含量在实验各时期内均低于Ⅰ组,24h时Ⅱ组与Ⅲ组、Ⅳ组之间差异均有统计学意义[(97.7±11.7) ng/L比(117.1±9.7) ng/L,( 124.3 ±4.9) ng/L,均P＜0.05],72、168 h时Ⅱ、Ⅲ、Ⅳ组肠静脉血内毒素含量差异无统计学意义.③24h时Ⅱ组TNF-α低于Ⅰ组及Ⅲ、Ⅳ组[(6.7±2.6) pg/100 μg比(13.5±1.7),(11.8±2.8),(12.4±2.7)pg/100 μg,均P＜0.05)],但72、168 h使用乌司他丁各组低于Ⅰ组.结论 乌司他丁对TNBS导致的大鼠UC有明显防护作用,其机制可能通过抑制内毒素水平而降低免疫异常水平.     

金阳乌头生物碱的研究

从金阳乌头根中分得四种生物碱:光翠雀碱(Ⅰ),14-乙酰新乌碱(Ⅱ),金乌碱(Ⅲ)(新的C₂₀二萜生物碱)和碱Ⅳ(结构待定)。药理试验显示Ⅰ对乌头碱引起的心率不齐有预防作用。     

千金藤属(Stephania)生物硷的研究——Ⅰ.地不容(S.epigeae)中生物硷的分离与鉴定

从地不容中分得四种生物硷,根据其理化性质及光谱数据,分别鉴定为轮环藤宁(Cycleanine)(Ⅰ),千金藤素(Cepharanthine)(Ⅱ),1-箭毒硷(1-Curine)(Ⅲ)和异紫堇定硷(isocorydine)(Ⅳ)。(Ⅱ)可用于防治辐射和化学物质引起的白细胞减少症,(Ⅰ),(Ⅲ)的碘甲烷化合物可作肌肉松弛剂,(Ⅲ)的作用强度与d-筒箭毒硷相似,其二甲醚碘甲烷衍生物的肌松作用较原硷碘甲烷盐强三倍。     

千金藤属(Stephania)生物硷的研究——Ⅰ.地不容(S.epigeae)中生物硷的分离与鉴定

从地不容中分得四种生物硷,根据其理化性质及光谱数据,分别鉴定为轮环藤宁(Cycleanine)(Ⅰ),千金藤素(Cepharanthine)(Ⅱ),1-箭毒硷(1-Curine)(Ⅲ)和异紫堇定硷(isocorydine)(Ⅳ)。(Ⅱ)可用于防治辐射和化学物质引起的白细胞减少症,(Ⅰ),(Ⅲ)的碘甲烷化合物可作肌肉松弛剂,(Ⅲ)的作用强度与d-筒箭毒硷相似,其二甲醚碘甲烷衍生物的肌松作用较原硷碘甲烷盐强三倍。     

茜草中萘酸酯类成分的研究

从茜草根中分离出四个萘酸甲酯化合物。经光谱分析及化学方法鉴定,化合物Ⅰ为一新成分,命名为茜草内酯(rubilactone,Ⅰ),化合物Ⅱ～Ⅳ分别为已知物3’-甲氧羰基-4’-羟基-萘骈[1’,2’-2,3]呋喃(furomollugin,Ⅱ),二氢大叶茜草素(dihydromollugin,Ⅲ),2-(3’-羟基)异戊基-3-甲氧羰基-1,4-萘氢醌-1-O-β-D-吡喃葡萄糖甙(Ⅳ)。其中Ⅱ系自茜草属中首次分得。     

复方乙酰水杨酸片灼烧物中止泻成分研究

从复方乙酰水杨酸片(APC)灼烧物中分离出六个化合物,其中五个化合物分别鉴定为阿斯匹林(Ⅰ),非那西丁(Ⅱ),水杨酸(Ⅲ),水杨酸乙酯(Ⅳ)及水杨酸苯酯(Ⅴ)。第六个化合物系苯酰苯胺衍生物,命名为 N-(4-乙氧苯基)-2-羟基苯酰胺(益肤酰胺,Ⅵ)。药理实验结果表明,化合物(Ⅰ),(Ⅴ)及 APC 片灼烧物具有明显的胃肠道蠕动抑制作用;益肤酰胺(Ⅵ)具有较强的免疫抑制作用。     

穆坪马兜铃化学成分的研究

自穆坪马兜铃(Aristolochia moupinensis Franch)根、茎中分得十三个化合物,其中化合物Ⅰ～Ⅻ经鉴定分別为马兜铃酸Ⅰ(aristolochic acid Ⅰ)(Ⅰ),尿囊素(allantoin)(Ⅱ),紫丁香酸(syringic acid)(Ⅲ),香豆酸(P-coumaric acid)(Ⅳ),马兜铃酸Ⅳ(aristolochic acid Ⅳ)(Ⅴ),β-谷甾醇(Ⅵ),木兰碱(magnoflorine)(Ⅶ),马兜铃酸Ⅳ甲醚aristolochic acid Ⅳ methyl ether(Ⅵ),棕榈酸(Ⅸ),马兜铃酸Ⅱ(aristolochic acid Ⅱ)(Ⅹ),N(Phydroxyphenethy1)P-coumaramide(Ⅺ),马兜铃酸Ⅳ甲醚甲酯(aristolochic acid Ⅳ methyl ether methyl ester)(Ⅻ),化合物ⅩⅢ为新化合物,经光谱分析和化学合成等方法证明其结构为N(P-hydroxyphenethyl)-ferulamide,命名为穆坪马兜铃酰胺(moupinamide)。 初步药理试验表明穆坪马兜铃酰胺体外有抑制血小板聚集和影响血小板内前列腺素合成的作用。     

The effect of continuous positive airway pressure on heart rate variability during the night in patients with chronic heart failure and central sleep apnoea

Continuous positive airway pressure (CPAP) improves autonomic activity in patients with chronic heart failure (CHF) and central sleep apnoea (CSA), but its effect on heart rate variability (HRV) during therapy has not been reported. We hypothesized that CPAP may decrease HRV, despite its beneficial effects on sympathetic overactivation, due to the expected stabilization of breathing. Sixty‐seven CHF patients underwent polysomnography (PSG). Ten of them presented with CSA (age 66.1±8.5 years, apnoea‐hypopnea index [AHI]=57.6±23.3, central AHI [cAHI]=41.6±24.6 [mean±SD]) and were subjected to a second PSG with manual CPAP titration. Beat‐to‐beat heart intervals for a 6‐hour period of sleep were extracted from each recording and HRV was analysed. CPAP significantly reduced AHI (AHI=23.1±18.3 P=.004). Standard deviation of normal‐normal interbeat interval (SDNN) (61.5±29.0 vs 49.5±19.3 ms, P=.021), root mean square of successive differences (RMSSD) (21.8±9.2 vs 16.4±7.1 ms, P=.042), total power (lnTP=7.8±1.1 vs 7.4±0.8 ms², P=.037), low frequency power (lnLF=5.5±1.5 vs 5.0±1.4 ms², P=.003) and high frequency power (lnHF=4.6±1.0 vs 4.0±1.0 ms², P=.024) were decreased. There was a strong correlation between the decrease in AHI and the decrease in lnHF (Spearman's ρ=.782). CPAP leads to a decrease in spectral and time domain parameters of HRV during therapy in CHF patients with CSA. These changes are best explained by the effect which CPAP‐influenced breathing pattern and lowered AHI exert on HRV.     

<Emphasis Type="Italic">In-vitro</Emphasis> evaluation of antioxidant potential of <Emphasis Type="Italic">rauwolfia vomitoria</Emphasis> root extract and its inhibitory effect on lipid peroxidation as indication of aphrodisiac properties

Rauwolfia vomitoria (RV) Afzel (Apocynaceae) is a medicinal plant used in traditional medicinal practice for the treatment of hypertension. This research is devoted to phytochemical constituents, in particular, some specific alkaloids present in the RV root extract. The phytochemical evaluation revealed the presence of alkaloids, tannins, saponins, and flavonoids in this extract. The antioxidant activity of the RV root extract was also evaluated in a series of in vitro assays involving free radicals. The extract exhibited significant hydrogen peroxide scavenging effect relative to ascorbic acid (p < 0.05, IC₅₀ = 98 μg/ml), nitric oxide scavenging effect (50.37 ± 0.4% after 150 min), and metal chelating activity (89.08 ± 2.62%). In addition, it exhibited significant ferric reducing power relative to ascorbic acid (p < 0.05). The total content of phenolic substances was 233.3 ± 2.9 mg/g. The extract was also studied for its inhibitory capacity on lipid peroxidation as a possible mechanism of its aphrodisiac effect, by measuring thiobarbituric acid reactive substances in various male-cow tissues incubated in a 5% solution of the RV root extract, distilled water, and antioxidant vitamins C and E upon keeping the samples frozen for 35 days. Tissues incubated in the test solution had lower levels of malondialdehyde (MDA) compared to those in the samples incubated in distilled water. Results obtained from this study indicate that the RV root extract can be a potential source of natural antioxidants.     

Toxicological effects of pet food ingredients on canine bone marrow‐derived mesenchymal stem cells and enterocyte‐like cells

We developed an in vitro method to assess pet food ingredients safety. Canine bone marrow‐derived mesenchymal stem cells (BMSC) were differentiated into enterocyte‐like cells (ELC) to assess toxicity in cells representing similar patterns of exposure in vivo. The toxicological profile of clove leave oil, eugenol, guanosine monophosphate (GMP), GMP + inosine monophosphate, sorbose, ginger root extract, cinnamon bark oil, cinnamaldehyde, thyme oil, thymol and citric acid was assessed in BMSC and ELC. The LC₅₀ for GMP + inosine monophosphate was 59.42 ± 0.90 and 56.7 ± 3.5 mg ml^–1 for BMSC and ELC; 56.84 ± 0.95 and 53.66 ± 1.36 mg ml^–1 for GMP; 0.02 ± 0.001 and 1.25 ± 0.47 mg ml^–1 for citric acid; 0.077 ± 0.002 and 0.037 ± 0.01 mg ml^–1 for cinnamaldehyde; 0.002 ± 0.0001 and 0.002 ± 0.0008 mg ml^–1 for thymol; 0.080 ± 0.003 and 0.059 ± 0.001 mg ml^–1 for thyme oil; 0.111 ± 0.002 and 0.054 ± 0.01 mg ml^–1 for cinnamon bark oil; 0.119 ± 0.0004 and 0.099 ± 0.011 mg ml^–1 for clove leave oil; 0.04 ± 0.001 and 0.028 ± 0.002 mg ml^–1 for eugenol; 2.80 ± 0.11 and 1.75 ± 0.51 mg ml^–1 for ginger root extract; > 200 and 116.78 ± 7.35 mg ml^–1 for sorbose. Lemon grass oil was evaluated at 0.003–0.9 in BMSC and .03‐0.9 mg ml^–1 in ELC and its mechanistic effect was investigated. The gene toxicology studies showed regulation of 61% genes in CYP450 pathway, 37% in cholestasis and 33% in immunotoxicity pathways for BMSC. For ELC, 80% for heat shock response, 69% for beta‐oxidation and 65% for mitochondrial energy metabolism. In conclusion, these studies provide a baseline against which differential toxicity of dietary feed ingredients can be assessed in vitro for direct effects on canine cells and demonstrate differential toxicity in differentiated cells that represent gastrointestinal epithelial cells. Copyright © 2015 John Wiley & Sons, Ltd.     

Anticancer effect of Cenchrus ciliaris L

Cenchrus ciliaris L total alcohol and successive extracts of both aerial and root parts were tested for their anticancer activities against lung (A-549), intestinal (CACO), colon (HCT-116), cervical (Hela), hepatocellular (HepG-2), and breast (MCF-7) (PC3) cell lines and compared with the standard drug vinblastine sulphate. The obtained results exhibited direct cytotoxic effect with variable inhibiting effect on the growth of the listed cell lines comparing to vinblastine sulphate as reference standard drug, these effects showed different IC₅₀ ranged from 11.1?±?0.3 to 267?±?µg/ml.All root extracts showed the best activities against most of the tested cell lines specially HepG-2 (Hepatocellular carcinoma) (9?±?2.1?µg/ml) which was somewhat closely related to the effect of vinblastine sulphate (2.93?±?0.3?µg/ml).The highest anticancer effect of Cenchrus ciliaris L aerial parts and root extracts were recorded on HepG-2 (Hepatocellular carcinoma) their IC₅₀ were 12?±?0.8 & 9?±?2.1 respectively, CACO (colorectal carcinoma) their IC₅₀ were 27.2?±?1.6 & 20.5?±?0.6 respectively, A-549 (Lung carcinoma) their IC₅₀ were 14.5?±?0.7& 11.1?±?0.3 respectively which were better than the standard drug especially in case the anticancer effect on CACO (colorectal carcinoma) and A-549 (Lung carcinoma). Chloroform extracts of both aerial and roots achieved the best anticancer activities on all of the cell lines especially with colorectal (CACO) and Lung carcinoma (A-549). Cenchrus ciliaris could be a promising source of new chemical moieties used to target cancer cells.     

Potential of Vitex negundo roots in the treatment of ulcerative colitis in mice

Context: Vitex negundo Linn. (Verbenaceae) is an indigenous tree species in India. This tree species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcer and colic pain.

Objective: The present work was undertaken to validate its folk use in the treatment of ulcerative colitis (UC) by using the method of acetic acid-induced colitis in mice.

Materials and methods: Ethanol and aqueous extracts of roots of V. negundo (100?mg/kg) were screened for use in the treatment of UC by the method of acetic acid-induced UC in mice. Macroscopical study of the colon, level of myeloperoxidase (MPO), malondialdehyde (MDA) in colon tissue and blood and histopathology of the colon tissue were studied for the assessment.

Results: Ethanol extract (100?mg/kg) reduced the level of MPO in blood from 355?±?0.39 to 240?±?0.36?U/mL and from 385?±?0.35 to 257?±?0.36?U/mg in tissue. Similarly, it reduced the level of MDA in blood from 9.40?±?0.42 to 6.10?±?0.36 nmol/mL and from 9.38?±?0.56 to 5.89?±?0.56?U/mg in tissue. Both the results are comparable with the standard drug, prednisolone (5?mg/kg). This preventive effect was observed by morphological and histopathological study.

Discussion and conclusion: Results showed that ethanol extract of V. negundo root is effective in the treatment of UC and results are comparable with the standard drug, prednisolone, and thus possessing a great potential in the treatment of UC.     

Six new C-21 steroidal glycosides from Dregea sinensis Hemsl

Six new C-21 steroidal glycosides (1–6) were separated from the root of Dregea sinensis Hemsl. and their structures were elucidated using extensive nuclear magnetic resonance, mass spectrometry, and infrared spectral analyses. Isolated compounds were evaluated for antitumor activity, which showed that compound 3 had moderate activity in Jurkat cells (IC₅₀ 19.54 ± 0.91 μM), and compounds 1–4 had significant effects against IL-2R and TNFR2 (IC₅₀ 1.518 ± 0.06 μM to 5.9 ± 0.07 μM).     

黄芪配伍熟地对去势大鼠骨质疏松的治疗作用

目的观察黄芪配伍熟地对去势大鼠骨密度及骨质病理改变的影响。方法采用切除雌性未孕大鼠两侧卵巢的方法制备去势大鼠骨质疏松模型。模型大鼠按照分组分别ig给予黄芪5.4g·kg-1,熟地5.4g·kg-1,黄芪+熟地(含黄芪2.7g·kg-1和熟地2.7g·kg-1),雌激素0.18mg·kg-1,每2周1次连续给药16周。制作骨骼切片,检测去势大鼠骨密度及骨质病理的改变。结果与假手术组比较,模型组的体质量显著增加,股骨质量〔(1.05±0.11)g〕明显降低,骨量丧失较为明显(P<0.05)。与模型组比较,黄芪组〔(373±63)g〕、熟地组〔(370±46)g〕及黄芪+熟地组的体质量〔(370±60)g〕均明显增加(P<0.05),但股骨量无显著变化;黄芪+熟地组的股骨密度(0.1470±0.0373)g·cm-1和椎骨骨密度(0.1350±0.0402)g·cm-1均明显增加(P<0.05),骨皮质厚度(0.852±0.151)g·cm-1及骨小梁直径(0.073±0.015)g·cm-1显著性增加(P<0.05)。结论黄芪配伍熟地能够增加骨密度、促进骨形成,使骨结构得到改善,对去卵巢大鼠骨质疏松具有一定的治疗作用。     

Enrichment of Bifidobacterium longum subsp. infantis ATCC 15697 within the human gut microbiota using alginate-poly-l-lysine-alginate microencapsulation oral delivery system: an in vitro analysis using a computer-controlled dynamic human gastrointestinal model

Abstract

This study evaluates alginate-poly-l-lysine-alginate Bifidobacterium longum subsp. infantis ATCC 15697-loaded microcapsules to enrich the human gut microbiota. The cell survival of alginate-poly-l-lysine-alginate microencapsulated B. infantis ATCC 15697 in gastric acid, bile, and through human gastrointestinal transit was investigated, as well as the formulation’s effect on the gut microbiota. Results show that microencapsulation increases B. infantis ATCC 15697 cell survival at pH1.0 (33.54?±?2.80% versus <1.00?±?0.00%), pH1.5 (41.15?±?2.06% versus <1.00?±?0.00%), pH2.0 (60.88?±?1.73% versus 36.01?±?2.63%), pH3.0 (75.43?±?1.23% versus 46.30?±?1.43%), pH4.0 (71.40?±?2.02% versus 47.75?±?3.12%) and pH5.0 (73.88?±?3.79% versus 58.93?±?2.26%) (p?<?0.05). In addition, microencapsulation increases cell survival at 0.5% (76.85?±?0.80% versus 70.77?±?0.64%), 1.0% (59.99?±?0.97% versus 53.47?±?0.58%) and 2.0% (53.10?±?1.87% versus 44.59?±?1.52%) (p?<?0.05) (w/v) bile. Finally, daily administration of alginate-poly-l-lysine-alginate microencapsulated B. infantis ATCC 15697 in a human gastrointestinal model induces a significant enrichment of B. infantis within the ascending (184.51?±?17.30% versus 53.83?±?17.82%; p?<?0.05), transverse (174.79?±?25.32% versus 73.17?±?15.30%; p?<?0.05) and descending (94.90?±?25.22% versus 46.37?±?18.93%; p?>?0.05) colonic microbiota.     

热牙胶充填与侧压充填治疗对急性牙髓炎和根尖周炎患者SBI、PD及GI值的影响

目的 分析热牙胶充填与侧压充填治疗对急性牙髓炎和根尖周炎患者龈沟出血指数(SBI)、牙周袋深度(PD)、牙龈指数(GI)值的影响.方法 选取在本院进行治疗的急性牙髓炎和根尖周炎患者84例,按随机数表法分为两组,每组42例.对照组实施侧压充填治疗,观察组采用热牙胶充填治疗.比较两组患者SBI、PD及GI值、疗效、根管充填时间及牙周疼痛情况.结果 治疗后,观察组SBI、PD、GI值分别为(1.6±0.5)mm、(4.0±0.4) mm、(1.4±0.3) mm,明显低于对照组的(2.4±0.5)mm、(5.8±1.0)mm、(2.1±0.4)mm,差异有统计学意义(P＜0.05);观察组根管充填时间为(17.5±4.1)min,明显短于对照组的(26.8±5.4) min,差异有统计学意义(P＜0.05);观察组治疗总有效率明显高于对照组(95.2％比78.6％),牙周疼痛情况明显改善(2t4％比19.0％),差异有统计学意义(P＜0.05).结论 热牙胶充填治疗急性牙髓炎和根尖周炎效果明显优于侧压充填治疗,可缩短患者根管充填时间,降低龈沟出血等指数,缓解牙周疼痛.     

Sulphation of resveratrol,a natural compound present in wine,and its inhibition by natural flavonoids

1. Resveratrol, a polyphenolic compound present in grape and wine, has beneficial effects against cancer and protective effects on the cardiovascular system. Resveratrol is sulphated, and the hepatic and duodenal sulphation might limit the bioavailability of this compound. The aim of this study was to see whether natural flavonoids present in wine, fruits and vegetables inhibit the sulphation of resveratrol in the human liver and duodenum. 2. In the liver, IC₅₀ for the inhibition of resveratrol sulphation was 12 ± 2 pM (quercetin), 1.0 ± 0.04 μM (fisetin), 1.4 ± 0.1 μM (myricetin), 2.2 ± 0.1 μM (kaempferol) and 2.8 ± 0.2 μM (apigenin). Similarly, in the duodenum, IC₅₀ was 15 ± 2 pM (quercetin), 1.3 ± 0.1 μM (apigenin), 1.3 ± 0.5 μM (fisetin), 2.3 ± 0.1 μM (kaempferol) and 2.5 ± 0.3 μM (myricetin). 3. The type of inhibition of quercetin on resveratrol sulphation was studied in three liver samples and was determined to be non-competitive and mixed in nature. K_m (mean ± SD; μM) was 0.23 ± 0.07 (control), 0.40 ± 0.08 (5 pM quercetin) and 0.56 ± 0.09 (10 pM quercetin). V_max (mean ± SD; pmol·min^?1·mg^?1) was 99 ± 11 (control), 73 ± 15 (5 pM quercetin) and 57 ± 10 (10 pM quercetin). K₁ and K_1es estimates (mean ± SD) were 3.7 ± 1.8 pM and 12.1 ± 1.7 pM respectively (p = 0.010). 4. Chrysin was a substrate for the sulphotransferase(s) and an assay was developed for measuring the chrysin sulphation rate in human liver. The enzyme followed Michaelis‐Menten kinetics and K_m and V_max (mean ± SD) measured in four livers were 0.29 ± 0.07 μM and 43.1 ± 1.9 pmol·min^?1·mg^?1 respectively. 5. Catechin was neither an inhibitor of resveratrol sulphation nor a substrate of sulphotransferase. 6. These results are consistent with the view that many, but not all, flavonoids inhibit the hepatic and duodenal sulphation of resveratrol, and such inhibition might improve the bioavailability of this compound.