The effects of metal ions on the biodegradation of benzene and toluene were investigated. Among 12 tested metal ions, Cu2+, Ni2+, Co2+ and A+g inhibited the degradation of benzene and toluene severely byAlcaligenes xylosaxidans Y234. Cu2+ was found to inhibit catechol 1,2-dioxygenase in the degradation of benzene and toluene. Co2+ and Ni2+ were supposed to inhibit benzoate 1,2-dioxygenase, while Ag+ was supposed to inhibit benzaldehyde dehydrogenase in the degradation of toluene. The inhibition effect caused by these metal
ions could be overcome both by microbial adaptation and by adding specific aromatic compounds to the broth. 相似文献
The Self-cycling fermentation (SCF) technique was applied to the biodegradation of several aromatic compounds by Pseudomonas putida and Pseudomonas fluorescens. The SCF technique was shown to have several advantages over the sequential batch reactor and other methods of biodegradation. The SCF technique allowed stable and repeatable performance over an extended period of time, with almost complete substrate consumption. The system was flexible and easily adapted to changes in concentration and/or type of substrate. The rates of consumption were considerably higher than reported for any other biodegradation technique. 相似文献
Strontium plays important physicochemical and biological roles in the applications of bone repair materials. The available methods of Sr doping in bone cements were believed to make a key effect on the biodegradation and Sr ion release behaviors of cements. In this work, Sr‐doped octacalcium phosphate (Sr‐OCP), Sr‐doped α‐tricalcium phosphate (Sr‐α‐TCP), SrCO3, and SrCl2 with different actual availability of Sr2+ were imported into α‐TCP bone cements, and their effects on the biodegradation and ions release of cements were comparatively investigated. Incorporation of different Sr carriers had led to distinct hydration morphologies, crystal evolutions, degradation rates, and microenvironments of bone cements during their in vitro biodegradation. Compared with other Sr carriers, Sr‐OCP facilitated the hydration reaction of α‐TCP, which induced the enhanced degradation and Sr ion release behaviors. In conclusion, Sr‐OCP was supposed to be a more potential Sr carrier applied in the synthesis of biodegradable Sr‐doped calcium phosphate bone cements. 相似文献
In the present investigation bio‐degradation of anthracene, a polyaromatic hydrocarbon, from its simulated mixture in methanol, has been studied using a monoculture strain, (Acinetobacter sp. (ATCC No. 14293)), within the concentration range 500‐800 mg/dm3. In a separate attempt bioremediation of diesel contaminated soil to reduce total aromatic content using the same bacterial strain has been carried out. The main emphasis of this investigation is to understand the complex reaction engineering behaviour involved in both the above bioprocess systems. It is observed that while Monod's classical substrate uninhibited model can be used for simulation purpose for the biodegradation of anthracene, the reaction engineering behaviour of the bioremediation of soil can be expressed by coupling Monod's classical equation with first order cell decay rate. In both the cases the concerned intrinsic kinetic parameters have been evaluated. 相似文献
Operation of the scanning electrochemical microscope used in feedback mode over a coated metal allows changes in the state of the coating surface to be monitored during immersion in aqueous electrolytes. This paper reports changes in the coating induced by specific anions in the electrolyte in situ during immersion. Significant surface roughening is observed for immersion times shorter than 1 day when the electrolyte contains chloride ions. This effect is also observed when the oxygen dissolved in the electrolytic phase is employed as redox mediator for SECM imaging. The coated system exposed to chloride-free electrolytes containing sulphate or nitrate maintains a featureless topography within the same time scale. The observed features are due to the nucleation and growth of blisters at the metal/coating interface induced by chloride ions in the environment. The implication is that ionic migration occurs simultaneously with the absorption of water by the coating already from the beginning of exposure to the aqueous environment. The unique role of chloride ions compared with sulphate or nitrate ions towards coating performance has been established at a very early stage following immersion of the sample. 相似文献
This study encompasses the biodegradation capacity of Anabaena fertilissima to model PAH (Polycyclic Aromatic Hydrocarbon) compounds namely Anthracene (ANT) and Pyrene (PYR) at different LC50 concentrations viz., 5.0 mg/L and 3.0 mg/L, respectively, on growth in terms of Chlorophyll-a and protein. Depletion in chlorophyll-a and protein content was registered with rise in PAHs concentration while the inhibition of nitrogen fixing enzymes such as nitrate reductase and glutamine synthetase activity was also concentration dependent and showed more sensitivity for high molecular weight aromatic compound PYR as compared to ANT. GC/MS analysis explained the degradation of ANT by 46% and PYR by 33%, at 5.0 mg/L and 3.0 mg/L, respectively. Moreover, the common degraded product for ANT was 2, 4-Dimethyl-1-heptene and for PYR it was 2, 3, 4-Trimethylhexane. Results indicate that PYR was more stable and recalcitrant compared to ANT. This study suggests that Anabaena fertilissima could be used for bioremediation of ANT and PYR pollution in surface waters and terrestrial environments. 相似文献
A promising gram-negative bacterial strain for the biodegradation of aniline as the sole carbon, nitrogen and energy sources was successfully isolated and identified as Delftia sp. XYJ6. The optimal temperature and pH for both the growth of Delftia sp. XYJ6 and the biodegradation of aniline were 30°C and 7.0, respectively. Initial aniline of 2000 mg8226;L-1 could be completely removed by the strain at 22 h, which showed that Delftia sp. XYJ6 had a strong ability in the biodegradation of aniline. It indicated that aniline was firstly converted to catechol catalyzed by aniline dioxygenase as a first product, which was then further biodegraded to cis,cis-muconic acid catalyzed by the catechol 1,2-dioxygenase of Delftia sp. XYJ6 as a second product. Cis,cis-muconic acid could also be further biodegraded to other small compound again. The pathway for the biodegradation of aniline by Delftia sp. XYJ6 was not previously reported. 相似文献
Out of 10 bacterial strains isolated out of wastewater of a pharmaceutical industry the most efficient bacterium, which could tolerate 5,000 mgl?1 of phenol in the minimal medium, was identified to be Corynebacterium sp.DST1 through 16S rDNA analysis. This strain could remove 99.4% of phenol from the minimal medium containing 4,000 mgl?1 of phenol. The ortho catalytic pathway using the enzyme catechol 1, 2- dioxygenase was found to be the major pathway for phenol degradation in the bacterium. The gene for catechol 1, 2-dioxygenase (catA) could also be amplified using degenerate primers. Corynebacterium sp.DST1 cells were attached to sawdust and removal of phenol from industrial effluents was evaluated. About 92.7% of phenol could be removed from the effluent of a heavy electrical industry. The results of this study not only suggest the potentiality of the strain to remove phenol efficiently from crude industrial effluents but also display a cheap method of bioremediation using sawdust. 相似文献
Abstract Thorium phosphate papers have been prepared by treatment with thorium nitrate and phosphoric acid solutions. Several metal ions have been chromatographed on thorium phosphate paper. The effect of pH on RF values has been investigated. Some useful analytical separations of metal ions have been achieved by using only dilute mineral acid and a mixed solvent system. 相似文献
Catechol was found to be a common intermediate in the degradation of benzene and toluene byAlcaligenes xyhsoxidans Y234, and the ring cleavage of the catechol mediated by catechol 1,2-dioxygenase was a rate-determining step. Since benzene
induced higher level of catechol 1,2-dioxygenase than toluene, the cells pre-adapted to benzene showed higher degradation
rate of benzene and toluene. The degradation rate ofm-xylene was also increased significantly when benzene-adapted cells were inoculated.m-Xylene was metabolized via 3-methyl catechol which was effectively cleaved by catechol 1,2-dioxygenase. 相似文献
Pt/Mg/Al metal oxide catalysts were prepared by impregnation and co-precipitation methods, respectively. These samples were
characterized by BET, XRD and NO-TPD; their NOX storage property and adsorbing intermediate species were investigated with NSC and FTIR. The results showed that the prepared
methods exert significant influence on the physical structure properties and the adsorption abilities of NO. (Pt)/Mg/Al samples
prepared by impregnation (IM) have larger specific areas and higher NOX storage capacity than (Pt)/Mg/Al catalysts prepared by co-precipitation (CP). The intermediate species of NO adsorbing process
indicated that NO was firstly adsorbed as bridged nitrites both on Pt/Mg/Al (IM) and on Pt/Mg/Al (CP), then on Pt/Mg/Al (IM)
the nitrites transferred into monodentate and bidentate nitrate species while on Pt/Mg/Al (CP) the nitrites only transferred
into monodentate nitrate species. 相似文献
A new mathematical model for n-alkane biodegradation in crude oil, heavy oil and paraffinic mixtures is described. The pattern of n-alkane degradation as a function of the inverse of hydrocarbon chain length reported in this paper can be considered as general behaviour for many aerobic n-alkane biodegradation processes. A new interpretation of n-alkane biodegradation as a function of surface tension, is given. A mathematical expression was obtained starting from the degradation values of n-alkane and relative surface tension, which is a parameter independent of fermentation conditions. An interesting parameter, b, was identified which represented the accelerating conversion factor for n-alkane biodegradation. The findings suggested that the n-alkane biodegradation. The findings suggested that he n-alkane biodegradation rate may be affected by the fermentation condition (agitation, aeration, etc.) and by the strain of microorganism, while the behaviour pattern of n-alkane degradation was essentially linked to the substrate characteristics (molecular structure, molecular weight and density). 相似文献
One native fungal strain, designated ZH-H2, was isolated from an agricultural soil contaminated by HMW-PAHs in a typical coal mining area of Hebei, China. The filamentous fungus was identified as a Fusarium sp. ZH-H2 was able to survive not only in the presence of the individual HMW-PAHs of Chry, BaA, B(K)F, BaP, DB(a,h)A, InP, InP and B(g,h,i)p but in the presence of a mixture of the above seven HMW-PAHs with a total concentration of up to 10 mg L?1. Biodegradation experiments demonstrated that Fusarium sp. (ZH-H2) was able to degrade the aforementioned individual HMW-PAHs, with a degradation percentage of 77%, 85%, 91%, 42%, 56%, 42% and 38%, respectively, and degrade the aforementioned seven PAHs mixture with a degradation percentage of 48%. The effect of starch addition on the biodegradation efficiency of the PAH mixture was also investigated. The results showed a significant improvement in the degradation extent of the PAH mixture with the increase of starch concentration. The greatest degradation rate (DR; 89%) in 7 d was obtained when starch was added at 1.0 g L?1, about 2-fold than was achieved without starch. This study implicates that Fusarium sp. (ZH-H2), a potential biodegrader, is suitable for practical field application in effective bioremediation of soils that have been simultaneously contaminated by several HMW-PAHs for a long time. 相似文献
The preparation of gelatinous aluminium hydroxide from aqueous solutions containing a mixture of the chloride, nitrate or sulphate of aluminium and urea by heating at 95°C has been investigated under different conditions. The pH value of aqueous solutions, on heating for a given period of time, gradually increases, rises steeply at pH 4.0–7.5 and finally approaches a constant value. The precipitate appears at about pH 7 in the presence of chloride or nitrate ions and about pH 4 in the presence of sulphate ions. Although the gelatinous precipitates in the chloride and nitrate systems are apparently different from the granular, filterable one in the sulphate system, their compositions are not influenced by the species of aluminium salt. The fresh precipitates exist in an amorphous state, and go to pseudoboehmite by ageing. It seems that the amount of pseudoboehmite increases as the concentration of aluminium salt in aqueous solution decreases. However, the transformation from amorphous aluminium hydroxide to pseudoboehmite is reduced in the presence of sulphate ions. Furthermore, it is found that the X-ray diffraction peak for the (200) plane, as an orthorhombic structure, in the pseudoboehmite precipitated from chloride or nitrate solution is more intense than that from sulphate solution. 相似文献
Heavy hydrocarbons (HHCs) in soils impacted by crude oil spills are generally recalcitrant to biodegradation due to their low bioavailability and complex chemical structure. In this study, soils were pretreated with varying concentrations of ultraviolet radiation A (UVA) or ultraviolet radiation C (UVC) activated titanium dioxide (TiO2) (1%–5%) under varying moisture conditions (0%–300% water holding capacity (WHC)) to enhance biodegradation of HCCs and shorten remediation timeframes. We demonstrate that pretreatment of impacted soils with UVC-activated TiO2 in soil slurries could enhance bioremediation of HHCs. Total petroleum hydrocarbon (TPH) removal after 24 h exposure to UVC (254 nm and 4.8 mW/cm2) was (19.1 ± 1.6)% in slurries with 300% WHC and 5 wt-% TiO2. TPH removal was non-selective in the C15-C36 range and increased with moisture content and TiO2 concentration. In a 10-d bioremediation test, TPH removal in treated soil increased to (26.0 ± 0.9)%, compared to (15.4 ± 0.8)% for controls without photocatalytic pre-treatment. Enhanced biodegradation was also confirmed by respirometry. This suggests that addition of UVC-activated TiO2 to soil slurries can transform recalcitrant hydrocarbons into more bioavailable and biodegradable byproducts and increase the rate of subsequent biodegradation. However, similar results were not observed for soils pretreated with UVA activated TiO2. This suggests that activation of TiO2 by sunlight and direct addition of TiO2 to unsaturated soils within landfarming setting may not be a feasible approach. Nevertheless, less than 1% of UVA (7.5 mW/cm2) or UVC (1.4 mW/cm2) penetrated beyond 0.3 cm soil depth, indicating that limited light penetration through soil would hinder the ability of TiO2 to enhance soil bioremediation under land farming conditions.
Due to natural attenuation, anionic detergents in surface waters are not inferred as big environmental issues. However, the effluents from large industrial areas with high detergent concentrations can have significant local impacts. These circumstances can be diminished by using efficient detergent‐degrading bacterial isolates through bioaugmentation. In this study, detergent plant effluents were analysed by using a methylene blue active substance assay to determine detergent content during natural attenuation processes, and after single augmentations of 12 anionic detergent‐degrading bacterial isolates with high detergent tolerating abilities in batch microcosms. Maximum bioattenuation of detergents was determined as 56 % after 66 h incubation under the conditions that mimicked the natural environment. Bioattenuation was enhanced as much as 83 and 91 % in 78 h incubation time through single microbial augmentations of filter‐sterilized and non‐sterilized effluents, respectively. Eight Pseudomonas and one Aeromonas species were found to be highly competitive by showing high biodegradation abilities in pure culture experiments as well as enhancing degradation of detergents in both filter‐sterilized and non‐sterilized effluents through their single augmentations. Although remaining three isolates, namely Pseudomonas fluorescens SDS6, P. resinovorans SDS10‐2, and P. corrugata SDS10‐3 displayed lower degrading abilities in pure culture experiments than the natural attenuation, they later turned out to be actively enhancing the degradation of detergents during their single augmentations. 相似文献