水稻施用硅肥效果

通过对盘山县的胡家、大荒、沙岭等农场施用硅肥效果调查 ,证明了施用硅肥对水稻的增产效果及抗逆能力 ;同时 ,分析论证了水稻施用硅肥增产、抗逆的机理     

水稻施用硅肥效果

通过对盘山县的胡家、大荒、沙岭等农场施用硅肥效果调查，证明了施用硅肥对水稻的增产效果及抗逆能力；同时，分析论证了水稻施用硅肥增产、抗逆的机理。     

硅肥在寒地水稻上追施效果试验总结

对寒地水稻进行不同用量追施硅肥试验,研究硅肥对水稻生长发育和产量的影响。结果表明:硅肥作为追肥施用后,水稻的生育期有所缩短,提高了水稻的抗性,平均产量增加4.06%,在4个处理中,以追施硅肥旺35 kg/667 m2增产效果最明显,增产5.18%。     

水稻施硅肥试验研究

对水稻施用硅(Si)肥进行试验研究,初步探索出水稻施用硅(Si)肥最佳量,及对水稻生育、产量的影响。结果表明:施用硅(Si)肥最佳量为25 kg/667 m~2,增产幅度列为第一位。硅(Si)肥一次施用可保持2 a,即降低成本,又有较好的经济收入。     

几种生物有机硅肥在水稻上应用效果研究

采用大区对比的方法,对八一农大、许博士、秦皇岛领先科技、佳木斯三兴4种生物硅肥开展了肥效对比试验。结果表明,施用硅肥能在一定程度上提高水稻抗病性,但效果不显著;施用硅肥能有效提高水稻产量构成因子,实现增产作用。四种硅肥中,八一农大生物硅肥增产效果最明显,基肥施硅处理产达605.5 kg/667 m^2,比对照增加55.4 kg/667 m^2。     

不同硅肥对水稻产量和抗病性的影响

为研究硅肥对水稻产量及抗病性的影响,特选取五种不同比例的硅肥进行对比试验。结果表明:水稻施用硅肥可增产0.73﹪～12.55﹪,使褐变穗病情指数下降9.16﹪～21.06﹪。综合效果看,"森屿牌"硅肥300㎏/hm2做底肥效果最好,增产955.35㎏/hm2,增产率为12.55﹪,增效1 624.10元/hm2。     

硅肥对本溪地区水稻生长的影响

从生育期、生物学特性、产量、抗倒伏、抗病虫等方面对水稻施用硅肥进行研究,结果表明,硅肥对水稻的增产效果明显,并在抗倒伏、抗病虫、生育期提前等方面也有较大作用。     

施用硅肥对水稻稻瘟病、生长及产量的影响

用3个不同的硅肥施用量对7个生产上的水稻主栽品种进行了稻瘟病、生长及产量田间试验,结果表明：施用硅肥对穗颈瘟具有一定的防治效果,对水稻的生长具有一定的促进作用和增产效应,但存在品种间差异,其中以施用225 kg/hm2效果最好;施用硅肥防控稻瘟病安全环保,有利于绿色食品的生产。     

水稻应用硅钙肥生产试验

开展硅钙肥对水稻的生育性状及产量影响试验研究,结果表明:硅钙肥对水稻具有一定的增产作用,使用本地常规硅肥相对于对照产量略有提高,在常规施肥的基础上施用硅钙肥5 kg/667 m~2产量最高,为483 kg/667 m~2,较对照增产5.7%,增效益59.8元/667 m~2。     

水稻施硅肥试验总结

通过对不同的硅肥、不同施入量的田间对比试验,结果表明:水稻施用硅肥,可提高整体素质,增产增收,稀林索硅肥用量50kg/667m2增产效果明显。     

Variation in Contents of Total Phenolics and Flavonoids and Antioxidant Activities in the Leaves of 11 <Emphasis Type="Italic">Eriobotrya</Emphasis> Species

Eriobotrya plants are known to have significant amounts of phenolics and flavonoids, and exhibit a strong antioxidant activity. Experiments were conducted to examine variation in the contents of total phenolics and flavonoids, and antioxidant activities in the leaves of 11 Eriobotrya species (Tibet loquat, Daduhe loquat, Hengchun loquat, Taiwan loquat, Oak leaf loquat, Bengal loquat, Fragrant loquat, Guangxi loquat, Obovate loquat, Big flower loquat, and common loquat, the last species include two materials, one is a cultivar ‘Zaozhong 6’, another is a wild tree). In these species, ‘Zaozhong 6′ loquat is a cultivar. The leaf extracts of ‘Tibet’, ‘Obovate’, ‘Taiwan’, ‘Bengal’ and ‘Hengchun’ loquats exhibited significantly higher contents of total flavonoids and total phenolics, compared with those of other species. Of these 11 species, the highest contents of total phenolics and total flavonoids were observed in ‘Tibet’ and ‘Obovatae’ loquats, respectively. The significantly stronger antioxidant abilities assessed by the DPPH radical scavenging activity and reducing power were obtained in the leaf extracts of ‘Taiwan’, ‘Tibet’, ‘Bengal’, ‘Oak leaf’, ‘Hengchun’ and ‘Obovate’ loquats, compared with the other species. In addition, significant correlations were found between the contents of total phenolics or flavonoids and DPPH radical scavenging activity/reducing power. This work indicates that the leaf extracts of the wild Eriobotrya species, ‘Tibet’, ‘Obovatae’, ‘Taiwan’, ‘Bengal’, ‘Oak leaf’ and ‘Hengchun’ loquats, exhibited significantly higher levels of total phenolics and flavonoids, and significantly stronger antioxidant activities, compared with the cultivated species, ‘Zaozhong 6′ loquat, which suggests that these wild species have a better utilization value.     

Quantifying the influence of sward height, concentrate level and initial milk yield on the milk production and grazing behaviour of continuously stocked dairy cows

Two factorial design experiments were carried out in the spring of 1994 and 1995, each of 6 weeks, to quantify the effects of sward height (SH), concentrate level (CL) and initial milk yield (IMY) on milk production and grazing behaviour of continuously stocked dairy cows. In Experiment 1, forty‐five Holstein Friesian cows were in five groups with initial milk yields of 16·9, 21·1, 28·0, 31·5 and 35·5 kg d^–1, grazed sward heights were 3–5, 5–7 and 7–9 cm (LSH, MSH and HSH respectively), and concentrates were fed at rates of 0, 3 and 6 kg d^–1. In Experiment 2, 48 cows were in two groups with IMY of 21·3 and 35·5 kg d^–1, grazed sward heights were 3–5 and 7–9 cm (LSH and HSH), and concentrates were fed at 0 and 6 kg d^–1 and ad libitum. Multiple regression models were used to quantify the effects of the three variables on milk yield persistency (MYP), estimated herbage dry‐matter (DM) intake (HDMI), grazing time (GT) and rate of DM intake (RI). The partial regression coefficients showed that increased SH led to increased MYP (Experiment 1 P < 0·001, Experiment 2 P < 0·05), increased HDMI (P < 0·01, P < 0·01), increased GT (P < 0·001, P < 0·05) and increased RI (P < 0·001, P < 0·05). Increasing CL led to increased MYP (NS, P < 0·001), decreased HDMI (P < 0·001, P < 0·001), decreased GT (NS, P < 0·001) and decreased RI (P < 0·001, P < 0·001). Higher IMY level of cows decreased MYP (P < 0·001, P < 0·001), increased HDMI (P < 0·001, P < 0·001), increased GT (P < 0·001, P < 0·05) and increased RI (P < 0·05, P < 0·01). The models were highly significant (P < 0·001), and accounted for 0·48–0·87 of the total variance. The partial regression coefficients quantified the extent to which GT and RI by cows respond positively to higher IMY, and negatively to increased CL, but respond differently (GT declines in response to a higher RI) with increasing SH.     

Inclination Angle Affects Ozone Injury in the Flag Leaf of Rice

Abstract

To evaluate the effect of inclination angle of the leaf on the leaf injury of rice by O₃, we examined the effect of a 5-hr exposure to 0, 0.1, 0.3 cm³ m-³ O₃, abbreviated as O⁰, O^0.1 and O^0.3, respectively, of the flag leaf using natural-light gas-exposure chambers. The middle portion of the flag leaf was set horizontally using lead weights (H plant) and it was compared with a non-treated, erect leaf (E plant). Gas exchange and chlorophyll fluorescence were measured immediately before, immediately after, and 1 and 3 d after the start of the exposure. The net photosynthetic rate, stomatal conductance and operating quantum efficiency of photosystem II (F_q’/F_m’) were decreased by the exposure to O₃. Furthermore, the inhibitory effects of O^0.1 and O^0.3 on the H plants were severer than those on the E plants. The maximum quantum efficiency of photosystem II (F_v/F_m) in the leaf of H plants decreased immediately after exposure to O₃. These results indicate that the weaker inhibitory effect of O₃ on the erect leaf depends on the lower light intensity at the leaf surface, rather than the horizontal leaf position.

Key words: Ascorbic acid, Chlorophyll fluorescence, Gas exchange, Leaf inclination angle, Oryza sativa, O₃, Quantum efficiency.     

Molecular phylogenetics of Pleosporales: Melanommataceae and Lophiostomataceae re-circumscribed (Pleosporomycetidae,Dothideomycetes, Ascomycota)

The classification of Pleosporales has posed major challenges due to the lack of clear understanding of the importance of the morphological characters used to distinguish between different groups in the order. This has resulted in varied taxonomic treatments of many families in the group including Melanommataceae and Lophiostomataceae. In this study we employ two nuclear DNA gene markers, nuclear ribosomal large subunit DNA and translation elongation factor 1-alpha in order to examine the molecular phylogenetics of Pleosporales with strong emphasis on the families Melanommataceae and Lophiostomataceae. Phylogenetic analyses recovered Melanommataceae, Lophiostomataceae, Hypsostromataceae, and a few others as strongly supported clades within the Pleosporales. Melanommataceae as currently circumscribed was found to be polyphyletic. The genera Byssosphaeria, Melanomma, and Pseudotrichia were recovered within the family, while others such as Ostropella and Xenolophium nested outside in a weakly supported group along with Platystomum compressum and Pseudotrichia guatopoensis that may correspond to the family Platystomaceae. The genus Byssosphaeria was recovered as a strongly supported group within the Melanommataceae while Melanomma was weakly supported with unclear relationships among the species. The genera Herpotrichia and Bertiella were also found to belong in the Melanommataceae. Lophiostomataceae occurs as a strongly supported group but its concept is here expanded to include a new genus Misturatosphaeria that bears morphology traditionally not known to occur in the family. The strongly supported clade of Misturatosphaeria contains nine species that have gregarious, papillate ascomata with lighter coloured apices and plugged ostioles and that vary in ascospore morphology from 1- to 3-septate to muriform. Along with a strongly supported Lophiostoma clade, also within the family are Thyridaria macrostomoides based on new sequences from Kenyan collections and Massariosphaeria triseptata, M. grandispora, Westerdykella cylindrica and Preussia terricola based on GenBank sequences. The family Hypsostromataceae was recovered as a strongly supported monophyletic group nested within the Pleosporales.Taxonomic novelties: Misturatosphaeria Mugambi & Huhndorf, gen. nov., M. aurantonotata Mugambi & Huhndorf, sp. nov., M. claviformis Mugambi & Huhndorf, sp. nov., M. cruciformis Mugambi & Huhndorf, sp. nov., M. kenyensis Mugambi & Huhndorf, sp. nov., M. minima Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. tennesseensis Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. uniseptata Mugambi, A.N. Mill. & Huhndorf, sp. nov., M. uniseriata Mugambi, A.N. Mill. & Huhndorf, sp. nov.     

Unravelling the phylogenetic relationships of lichenised fungi in Dothideomyceta

We present a revised phylogeny of lichenised Dothideomyceta (Arthoniomycetes and Dothideomycetes) based on a combined data set of nuclear large subunit (nuLSU) and mitochondrial small subunit (mtSSU) rDNA data. Dothideomyceta is supported as monophyletic with monophyletic classes Arthoniomycetes and Dothideomycetes; the latter, however, lacking support in this study. The phylogeny of lichenised Arthoniomycetes supports the current division into three families: Chrysothrichaceae (Chrysothrix), Arthoniaceae (Arthonia s. l., Cryptothecia, Herpothallon), and Roccellaceae (Chiodecton, Combea, Dendrographa, Dichosporidium, Enterographa, Erythrodecton, Lecanactis, Opegrapha, Roccella, Roccellographa, Schismatomma, Simonyella). The widespread and common Arthonia caesia is strongly supported as a (non-pigmented) member of Chrysothrix. Monoblastiaceae, Strigulaceae, and Trypetheliaceae are recovered as unrelated, monophyletic clades within Dothideomycetes. Also, the genera Arthopyrenia (Arthopyreniaceae) and Cystocoleus and Racodium (Capnodiales) are confirmed as Dothideomycetes but unrelated to each other. Mycomicrothelia is shown to be unrelated to Arthopyrenia s.str., but is supported as a monophyletic clade sister to Trypetheliaceae, which is supported by hamathecium characters. The generic concept in several groups is in need of revision, as indicated by non-monophyly of genera, such as Arthonia, Astrothelium, Cryptothecia, Cryptothelium, Enterographa, Opegrapha, and Trypethelium in our analyses.     

Antihyperglycemic,Antioxidant and Antiglycation Activities of Mulberry Leaf Extract in Streptozotocin-Induced Chronic Diabetic Rats

In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33 and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A_1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group (6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC₅₀ of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic diabetic rats, which may be beneficial as food supplement for diabetics.     

Time-mortality relationships of larvae and adults of grain beetles exposed to extreme cold

Mortality effects of low temperature exposure to −16 °C were investigated on larvae and adults of the grain beetles Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) and Trogoderma granarium (Everts) (Coleoptera: Dermestidae), in the laboratory. The main effects and interactions of exposure time (0.2, 0.5, 1, 2, 4, 8, 12, 24 and 48 h), developmental stage (larva and adult) and individual’s age (young and old) were examined. After 4 h of exposure 100% mortality has been achieved in most of the treatments. The only exceptions were that of old larvae of T. granarium and O. surinamensis, and adults of T. granarium which needed 48, 8, and 24 h, respectively. T. granarium was the most cold-hardy species irrespective of developmental stage, and age, followed in decreasing order by O. surinamensis and T. confusum. Younger adults were generally more susceptible to cold in the cases of O. surinamensis and T. confusum. On the contrary, older adults of T. granarium suffered higher mortality than younger ones. Larvae of T. granarium were generally more cold-tolerant than adults but the opposite pattern was observed in O. surinamensis and T. confusum. Main effects of exposure time, developmental stage and individual’s age on mortality proved to be significant for all species, with the exception of T. granarium where the effect of developmental stage proved to be insignificant. Lethal time values were estimated via probit analysis. Values of LT₅₀ ranged between 0.7 and 1.0 h for T. confusum, 0.7-1.9 h for O. surinamensis and 1.2-3.4 h for T. granarium. Our results are discussed with findings from relevant studies and the possibility of including extreme cooling to IPM programs against stored product pests is investigated.     

Dissection of antioxidant activity of durum wheat (Triticum durum Desf.) grains as evaluated by the new LOX/RNO method

Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative LOX/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay, one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and lipophilic compounds were extracted from eight different whole flour samples; extracts were analyzed for AA and their content in several antioxidants. The LOX/RNO method measured very high AA values, with the highest ones [850–1500 μmol Trolox eq./g whole flour (dry weight)] for insoluble-bound phenolic extracts, highly correlated to total phenolic (r = 0.761, P < 0.001) and ferulic acid (r = 0.816, P < 0.001) contents. Hydrophilic and lipophilic extracts showed lower AA [70-140 and 40–60 μmol Trolox eq./g (dry weight), respectively], highly correlated to flavonoid (r = 0.583, P < 0.01) and protein (r = 0.602, P < 0.01), as well as β-tocotrienol (r = 0.684, P < 0.05) contents, respectively. Interestingly, the LOX/RNO method suggests that insoluble-bound phenolic compounds may exert very strong synergistic interactions within the extract. Contrarily, the TEAC assay did not correlate to any antioxidant content, resulted unable to highlight differences among samples, measured much lower AA values and did not suggest synergism. The use of the LOX/RNO method is useful to unearth new properties of phytochemicals from durum wheat grains, potentially giving health benefits.