人脑星形细胞瘤环氧化酶-2的表达及与细胞增殖凋亡的关系

目的:检测人脑星形细胞瘤环氧化酶-2(cyclooxygenase-2, COX-2)表达,探讨COX-2与肿瘤恶性程度、细胞增殖活性及凋亡程度的关系.方法:采用免疫组化方法检测星形细胞瘤组织中COX-2及增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)、Mcl-1癌基因的表达情况.结果: 68例星形细胞瘤组织COX-2的表达阳性率为61.8%,COX-2的表达强度和病理分级呈正相关(χ2=14.24, P＜0.01).COX-2与Mcl-1、PCNA蛋白表达均呈正相关性.结论:COX-2蛋白在星形细胞瘤过度表达与病理分级及细胞增殖、凋亡关密切相关.     

星形细胞瘤发生的分子生物学机制及与COX-2 mRNA表达的相关性

目的:探讨环氧合酶2(COX-2)mRNA的表达与星形细胞瘤发生、发展的关系以及星形细胞瘤发生的分子生物学机制。方法:应用RT-PCR技术检测各级别星形细胞瘤组织及非肿瘤脑组织中COX-2mRNA的表达水平。结果:COX-2mRNA表达阳性率肿瘤组织为83.3%,非肿瘤脑组织为28.6%;COX-2mRNA的相对含量肿瘤组织为119.67±46.56,非肿瘤脑组织为43.14±19.26;COX-2mRNA在肿瘤组织中的表达显著高于其在非肿瘤脑组织中的表达(P<0.05)。结论:COX-2mRNA表达与星形细胞瘤的发生、发展呈显著正相关,其过表达是星形细胞瘤的重要分子生物学特征。     

星形细胞瘤中环氧化酶-2 mRNA及蛋白表达的意义

目的 :探讨星形细胞瘤组织中环氧化酶 2 (COX2 )蛋白、mRNA的表达及其与病理级别的相关性。方法 :用原位杂交的方法检测星形细胞瘤组织中COX2mRNA的表达情况 ;用免疫组化SP法检测同等标本中COX2蛋白的表达情况 ;并将表达情况与星形细胞瘤病理级别作相关性分析。结果 :COX2蛋白在星形细胞瘤表达的染色积分为 4 .5 6± 2 .32 ,正常脑组织染色积分为 1.2 1± 1.18,二者相比较有显著性差异 (P <0 .0 1) ;低度恶性组(Ⅰ ,Ⅱ级 )染色积分为 1.84± 1.4 6 ,高度恶性组 (Ⅲ ,Ⅳ级 )染色积分为 6 .37± 3.6 2 ,二者相比较有显著性差异 (P <0 .0 1)。COX2mRNA在星形细胞瘤表达率为 6 2 .6 9% ,在正常脑组织中呈阴性表达 ,二者比较有显著性差异 (P <0 .0 1) ;COX2mRNA在低度恶性组和高度恶性组中表达率分别为 37.14 % ,87.5 % ,二者比较有显著性差异 (P <0 .0 1)。结论 :COX2蛋白、mRNA在星形细胞瘤组织中高度表达 ,且表达率与病理级别密切相关 ;从基因水平和蛋白水平说明COX2基因在星形细胞瘤的生长和病理进程中发挥着重要作用。     

人脑胶质瘤中环氧化酶-2的表达和作用研究

目的研究环氧化酶-2（COX-2）在人脑胶质瘤中的表达状况，探讨其作用。方法对62例脑胶质瘤患者和3例脑外伤患者（对照组）运用免疫组化及Western blot方法检测COX-2蛋白表达。结果在正常脑组织中，仅少数神经细胞COX-2染色阳性；在脑胶质瘤中，都发现有较多肿瘤细胞COX-2染色阳性。脑胶质瘤中COX-2染色的表达强度与胶质瘤的病理分级呈正相关（1=-0．874，P〈0．01）。正常脑组织与胶质母细胞瘤、间变性星形细胞瘤和星形细胞瘤之间，COX-2表达强度的差异均有统计学意义（均P〈0．01）。在坏死区域周围肿瘤细胞有COX-2的高表达。正常脑组织与肿瘤组织的新生血管内皮细胞发现有COX-2染色阳性，在血管周围有COX-2染色阳性的肿瘤细胞聚集。结论COX-2在各级别的脑胶质瘤细胞中有表达，和脑胶质瘤的发生和发展有关系；COX-2在肿瘤的坏死区周围有强表达，在肿瘤细胞的凋亡和死亡过程起作用；COX-2在肿瘤的新生血管内皮上有表达，在血管周围有COX-2阳性的肿瘤细胞积聚，可促进肿瘤组织新生血管的生成；COX-2可作为判断脑胶质瘤恶性程度的辅助生物学标志。     

星形细胞肿瘤中RCAS1的mRNA及蛋白表达的临床意义

目的:探讨RCAS1在人星形细胞肿瘤中的基因及蛋白表达,及其与肿瘤发生、发展的关系.方法:RT-PCR法检测59例新鲜星形细胞肿瘤组织及6例正常脑组织中RCAS1的mRNA表达.免疫组织化学法检测71例星形细胞肿瘤及6例正常脑组织病理切片中RCAS1的蛋白表达.结果:弥漫型星形细胞瘤(GradeⅡ)和间变型星形细胞瘤之间(Grade Ⅲ)、间变型星形细胞瘤和胶质母细胞瘤(Grade Ⅳ)之间RCAS1 mRNA的表达有统计学差异(P＜0.05),而RCAS1蛋白表达仅在间变型星形细胞瘤和胶质母细胞瘤之间存在统计学差异(P＜0.01),RCAS1蛋白表达与肿瘤级别呈正相关(r=0.573,P＜0.001).在正常脑组织中未检测到RCAS1蛋白.结论:RCAS1的表达与星形细胞肿瘤的病理分级有关.星形细胞肿瘤中RCAS1的表达在转录和转录后水平受到调节.     

Oncol2012: RNA结合蛋白RBM3在人脑星形细胞瘤中的表达研究

背景：在低温和缺氧诱导的条件下,RNA结合蛋白RBM3（RNA结合基序蛋白3）的转录水平升高,近些年来在人类许多系统肿瘤中发现其转录水平也发生上调。然而,其在人脑星形细胞瘤中的表达情况尚缺乏相关研究,本文主要针对RBM3在不同病理级别星形细胞瘤和正常脑组织中的表达情况进行实验研究。 方法：本实验采用实时定量PCR,免疫组化和蛋白印迹的方法分别对RBM3 mRNA及蛋白水平进行检测。统计学方法采用方差分析（ANOVA）对实时定量PCR的数据进行分析,表示为均值?标准差。采用Fisher确切概率法用于免疫组化结果的分析。其中P值小于0.05,认为差异有统计学意义。 结果：一方面,首先对X-染色体相关的RBM基因（RBMX,RBM3,RBM10）进行实时定量PCR检测,结果表明,RBMX和RBM10的 mRNA水平在不同级别的星形细胞瘤和正常脑组织中无显著性差异。然而,实时定量PCR结果显示在高级别星形细胞瘤中RBM3 mRNA水平上调,其中高级别（WHO III-IV级）与低级别（WHO I-II级）星形细胞瘤相比差异有统计学意义（5.06?0.66比1.60?0.58,P<0.05）,高级别星形细胞瘤与正常脑组织相比差异也存在统计学意义（5.06?0.66比1.03?0.22,P<0.05）。另一方面,免疫组化结果显示RBM3在星形细胞瘤和正常脑组织中呈细胞核染色,并且随着病理级别的升高其表达水平也逐渐增高（阳性率：星形细胞瘤Ⅳ级92.9％,Ⅲ级81.8％,I-II级：50％,正常脑组织：37.5％;高级别星形细胞瘤与正常脑组织相比：P <0.05;高级别与低级别星形细胞瘤相比：P<0.05）。同时蛋白印迹的结果再次验证了RBM3在高、低级别星形细胞瘤与正常脑组织中的表达差异变化趋势。 结论：本研究初步认为RBM3在人脑星形细胞瘤和正常脑组织中存在一定程度的表达差异,并且随着星形细胞瘤恶性程度的加剧其表达逐渐增高提示其可能影响星形细胞瘤形成、恶性进展及预后等诸多环节。     

环氧合酶-2在星形细胞肿瘤组织中的表达及临床意义

目的探讨COX-2的表达与星形细胞肿瘤发生、发展的关系。方法应用免疫组化技术和RT-PCR技术检测各级别星形细胞肿瘤组织及非肿瘤组织中COX-2蛋白和mRNA的表达水平。结果COX-2蛋白在各组中的表达强度(IODA值)分别为:非肿瘤组织(2.19±0.88),肿瘤组织(6.38±3.68);COX-2mRNA在各组中的表达阳性率分别为:非肿瘤组织(33.3%),肿瘤组织(83.3%);COX-2mRNA的相对含量分别为:非肿瘤组织(43.49±19.73),肿瘤组织(119.67±46.56);COX-2在肿瘤组织中的表达显著高于其在非肿瘤组织中的表达(P<0.05)。结论COX-2的过表达与星形细胞肿瘤的发生、发展关系密切,是引起肿瘤细胞恶性表型的相关基因之一;毛细胞型星形细胞瘤与弥漫性浸润性星形细胞肿瘤的遗传基础不同,是一种在生物学和形态学上所发生的基因改变不同于其它胶质瘤的星形细胞肿瘤;COX-2除参与肿瘤形成、炎症的发生等病理过程外,在人体器官发育及正常生理功能的维持过程中也起到比预期更为复杂的作用。     

人脑星形细胞瘤组织Survivin与VEGF表达及意义

目的 探讨凋亡抑制基因Survivin与血管内皮生长因子(VEGF)在人脑星形细胞瘤中的表达及意义.方法 采用免疫组织化学SP法检测60例人脑星形细胞瘤组织和12例正常脑组织中Survivin、VEGF基因的表达,并分析其与临床病理特征的关系以及两者间的相互关系.结果 正常脑组织中仅2例VEGF呈弱阳性表达,阳性率为16.7%;Survivin均未见阳性表达.人脑星形细胞瘤组织中Survivin、VEGF表达的阳性率分别为63.3%(38/60)、70.0%(42/60), 其表达强度均显著强于正常脑组织(Uc=3.668、3.403,P<0.01).高级别星形细胞瘤Survivin、VEGF的表达阳性率及阳性强度均高于低级别组(χ2=4.341、6.609,Uc=2.318、3.141;P<0.05、0.01).Survivin、VEGF在脑星形细胞瘤组织中的表达呈显著正相关(rs=0.71,P<0.001).结论 Survivin、VEGF的表达与人脑星形细胞瘤的发生发展有关,且二者具有正相关性;二者的检测可能成为评价脑星形细胞瘤恶性程度与预后的有价值指标.     

人脑星形细胞瘤组织中转化生长因子α与肿瘤增殖细胞核抗原检测

目的:探讨人脑星形细胞瘤组织中转化生长因子α(TGF-α)蛋白的表达与肿瘤增殖细胞核抗原(PC-NA)及预后的关系.方法:采用免疫组化SP法检测50例人脑星形细胞瘤(其中星形细胞瘤18例,间变性星形细胞瘤20例,多形性胶质母细胞瘤12例)石蜡标本和10例正常脑组织中TGF-α蛋白表达和PCNA标记指数(PCNA-LI),并随访比较50例人脑星形细胞瘤患者术后1 a的生存率.结果:10例正常脑组织中TGF-α、PCNA-LI蛋白无表达;50例人脑星形细胞瘤组织中TGF-α蛋白表达总阳性率为60.0%(30/50),总PCNA-LI为(67.86±28.68)%;随着肿瘤病理分级的增高,TGF-α蛋白表达和PCNA-LI也显著增高,各级别组间差异有统计学意义(P＜0.05);TGF-α蛋白表达和PCNA-LI呈正相关(r=0.864,P＜0.01),TGF-α蛋白表达强弱和PCNA-LI高低反映患者预后(P＜0.01).结论:人脑星形细胞瘤中存在TGF-α和PCNA蛋白高表达,联合检测TGF-α蛋白和PCNA-LI对评估人脑星形细胞瘤的生物学行为具有重要意义.     

脑星形细胞瘤组织p73和p53蛋白的表达

目的 :探讨脑星形细胞瘤组织p73和p5 3蛋白的表达及其与星形细胞瘤的病理发生的关系。方法 :利用免疫组化方法检测了 6 3例脑星形细胞瘤组织 (I级 8例 ,II级 2 1例 ,III级 2 4例 ,IV级 10例 )及 10例正常脑组织p73、p5 3蛋白表达。结果 :17例脑星形细胞瘤组织呈p73蛋白阳性表达 ,高病理级别的星形细胞瘤组织p73蛋白阳性表达率 (III、IV级 ,38.2 % )明显高于低病理级别的星形细胞瘤组织 (I、II级 ,13.8% ) (χ2 =4.75 ,P <0 .0 5 ) ,10例正常脑组织均呈阴性表达。在 17例p73蛋白阳性表达的脑星形细胞瘤组织中 ,12例呈p5 3蛋白过度表达。结论 :野生型p73蛋白表达异常可能参与脑星形细胞瘤的病理发生 ,p5 3突变可能与p73表达异常有关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.