ERβ蛋白和COX-2蛋白在结直肠癌中的表达及临床意义

目的:探讨雌激素受体β(ERβ)和环氧化酶-2(COX-2)在结直肠癌中的表达及其临床意义。方法:采用免疫组化法检测ERβ、COX-2在62例结直肠腺癌组织标本中的表达,以其中大肠腺瘤13例及远癌正常大肠组织10例作对照。结果:ERβ及COX-2在远癌组织中阳性率低于腺瘤性息肉组及腺癌组的表达,其中远癌组织组与腺癌组有统计学差异(P<0.05),腺瘤性息肉组与腺癌组表达率无统计学差异(P>0.05)。在结直肠癌病例中ERβ、COX-2表达与结直肠癌患者性别、肿瘤分化程度、肿瘤浸润层次无关(P>0.05),而与Dukes分期、有无转移淋巴结相关。结论:ERβ、COX-2在正常组织、腺瘤性息肉及腺癌中的表达逐步上调,ERβ和COX-2在结直肠癌组织中表达明显升高,可能促进了大肠癌的转移、进展。ERβ、COX-2蛋白在结直肠癌的演进中起着协同作用。     

环氧化酶-2基因在肺癌组织中的表达及其意义

目的:研究环氧化酶-2(COX-2)基因在肺癌中的表达及其与临床病理特征之间的关系。方法:RT-PCR检测56例肺癌组织及相对应的癌旁正常组织、12例肺良性病变组织的COX-2mRNA表达。结果:肺癌组织COX-2基因表达阳性率(60.7%)高于相应癌旁正常组织(10.7%)和肺良性病变(25.0%)(P<0.01;P<0.05),腺癌高于鳞癌(P<0.01),但与患者的年龄、性别、TNM分期及肿瘤组织的分化程度无明显相关(P>0.05)。结论:COX-2基因在肺癌组织尤其是肺腺癌中表达上调,提示该基因对肺癌的发生、发展起重要作用,COX-2基因有望成为肺癌基因治疗的靶位点。     

大肠癌组织COX-2的表达及其与肿瘤细胞增生和凋亡的关系

目的 探讨大肠癌中环氧化酶-2（c yclooxygenase-2,COX-2）表达及其与癌细胞增生和凋亡的关系.方法 采用免疫组织化学法检测60例大肠癌、20例大肠腺瘤和20例癌旁正常大肠黏膜中COX-2和PCNA的表达,并采用TUNEL法检测原位细胞凋亡水平.结果 大肠癌组织中COX-2阳性表达、PI及AI均显著高于大肠腺瘤及正常大肠黏膜组织（P＜0.05）;COX-2的高表达与大肠癌肿瘤大小、淋巴结转移和临床分期有关,与肿瘤生长部位、分化程度无关.COX-2阳性大肠癌组中PI高于COX-2阴性大肠癌组,AI低于COX-2阴性大肠癌组（P＜0.05）.结论 COX-2的过度表达可促进大肠癌细胞增生、抑制细胞凋亡,在大肠癌的发生、发展过程中起着重要的作用.     

HER-2/neu COX-2 PGE2 P450arom在子宫内膜癌组织的表达及其相关性研究

探讨子宫内膜腺癌组织中HER-2/neu、COX-2、PGE2、P450arom 4种生物学指标的表达,分析这4种生物学指标表达强度之间相关性及与临床病理因素的相关性。方法:应用逆转录-聚合酶链反应（RT-PCR）技术及酶联免疫吸附试验（ELISA）检测HER-2/neu、COX-2、P450arom及PGE2在正常子宫内膜、子宫内膜不典型增生和子宫内膜腺癌组织中的表达情况。结果:HER-2/neu、COX-2、P450arom及PGE2在癌组织中表达高于非癌组织,有显著性差异（P<0.01）。癌组织中HER-2/neu与COX-2、P450arom表达强度呈正相关（r值分别为0.931,0.934,P<0.05）,COX-2与P450arom表达强度亦呈正相关（r=0.908,P<0.05）。COX-2mRNA和PGE2表达强度变化呈正相关（r=0.552,P<0.05）。结论:HER-2/neu、COX-2、P450arom及PGE2可能存在协同作用,共同促进子宫内膜腺癌的发生发展。      

非小细胞肺癌患者肺组织环氧合酶-2表达的变化及其临床意义

目的探讨非小细胞肺癌(NSCLC)患者肺组织中环氧合酶-2(COX-2)的表达和血浆PGE 2含量的变化及其与临床病理参数之间的关系.方法采用逆转录聚合酶联反应(RT-PCR)和放射免疫法,分别检测38例NSCLC患者和正常健康者肺组织中COX-2 mRNA的表达以及血浆PGE 2含量的变化.结果 38例肺癌患者肺癌组织和正常肺组织中分别有34例和3例检测到COX-2 mRNA表达,阳性表达率分别为13.16%和89.47%;半定量分析肺癌组织中的COX-2 mRNA平均吸光度显著高于正常肺组织(P＜0.001).肺癌患者和正常健康者血浆中PGE 2浓度分别为(42.33±6.13)μg/L和(5.87±1.40)μg/L,二者比较,差异亦具有显著性(P＜0.01).直线相关分析肺癌组织中COX-2 mRNA的表达和PGE 2浓度的变化呈正相关(r=0.552,P＜0.05).COX-2 mRNA表达在不同性别、年龄、组织分型、TNM分期和分化程度之间差异分别均无显著性(P＞0.05).结论 NSCLC患者肺癌级织中COX-2 mRNA和PGE 2表达增强,可能在NSCLC的发生和发展中具有重要作用.     

环氧合酶-2 mRNA在胃腺癌组织中表达的实验研究

目的探讨环氧合酶-2(COX-2)mRNA在胃腺癌组织及癌旁的正常胃粘膜组织中的表达及其意义.方法应用半定量逆转录-多聚酶链反应(RT-PCR)检测胃腺癌组织及病灶旁的正常胃粘膜组织中COX-2 mRNA的表达水平.应用Molecular Analyst软件定量分析RT-PCR产物电泳带,COX-2指数由COX-2和β-actin条带的积分吸光度值的比值来确定.COX-2指数大说明组织中COX-2 mRNA的表达水平高.结果胃腺癌组织的COX-2指数明显高于正常胃粘膜(P＜0.01).有淋巴结转移的胃癌组织COX-2指数显著高于无淋巴结转移者(P＜0.01);Ⅲ期和Ⅳ期的COX-2指数分别明显高于Ⅰ期及Ⅱ期(P＜0.05及P＜0.05).癌组织中COX-2 mRNA的表达水平与肿瘤的Borrmann分型、肿瘤浸润深度、分化程度、有无远处转移及肿瘤长径间无相关性(P＞0.05).结论 COX-2 mRNA的表达水平在胃腺癌组织中明显增高;其高表达与淋巴转移及临床TNM分期相关.     

COX-2、CD4及CD8在胃癌组织中的表达及其意义

目的探讨胃癌中COX-2、CD4及CD8表达的关系及意义。方法采用免疫组织化学SP法检测62例胃腺癌、22例腺上皮异型增生组织及20例正常胃组织中COX-2、CD4及CD8的表达,并分析其与临床病理特征的关系。结果腺癌组织与腺上皮异型增生组织的COX-2表达高于正常胃组织,差异有统计学意义(P<0.05);腺癌组织与腺上皮异型增生组织的CD4、CD8表达低于正常胃上皮组织,差异有统计学意义(P<0.05);胃癌中淋巴结转移者及分化较低者COX-2表达低(P<0.05);COX-2与CD4及CD8的表达呈负相关。结论 COX-2高表达可能是胃癌变过程中的早期事件。COX-2促进胃癌变过程可能与其抑制机体免疫反应有关。     

大肠癌中Fas、FasL、bcl-2蛋白的表达及意义

 目的　探讨Fas、FasL、bcl 2在大肠癌中表达及意义。方法　用免疫组化S P法检测 15例大肠腺瘤、4 0例大肠腺癌及 15例大肠正常组织Fas、FasL、bcl 2蛋白表达 ,以TUNEL法测定细胞凋亡。结果　腺瘤和腺癌凋亡指数高于正常组织 ,腺瘤组高于腺癌组 (P <0 .0 5 )。在大肠正常组织、腺瘤、腺癌中Fas阳性率下降 ,FasL阳性率上升 ,有显著性差异 (P <0 .0 5 )。腺瘤组和腺癌组bcl 2表达高于正常组 (P <0 .0 5 )。bcl 2阳性组FasL阳性率高于bcl 2阴性组 (P <0 .0 5 )。结论　瘤细胞Fas FasL表达异常使肿瘤逃脱机体自身免疫攻击 ,促使肿瘤的发生、发展 ,对预测大肠癌的预后有重要参考价值。bcl 2高表达是癌变过程中的早期事件 ,有利肿瘤的早期诊断。     

胃癌组织和胃周淋巴结COX-2 mRNA的表达及其意义

目的:探讨胃癌组织和胃周淋巴结内环氧化酶-2(COX-2)mRNA的表达特点及其意义。方法:选取胃癌患者43例和正常胃粘膜组织15例,取癌灶、癌旁、淋巴结和正常胃粘膜标本,通过采用PCR(Real-timeSemi-quantitativePCR)方法检测COX-2mRNA表达。结果:1)癌灶处COX-2mRNA表达高于癌旁和正常胃粘膜组织的表达;2)癌旁COX-2mRNA表达高于正常胃粘膜组织的表达(P<0.05);3)侵透浆膜的胃癌组织COX-2mRNA表达高于未透浆膜的表达;4)弥漫生长的胃癌组织COX-2mRNA表达高于团块状及巢状生长者的表达(P<0.05);5)淋巴结转移阳性的淋巴结组织COX-2表达高于淋巴结转移阴性的表达(P<0.05)。结论:胃癌组织COX-2mRNA的表达明显升高,其表达与胃癌生物学行为相关;胃癌周围淋巴结组织COX-2mRNA的表达情况可作为推测癌周淋巴结有无微小转移癌的一个具有提示意义的参考指标。     

NF-кB和CXCR4在大肠腺癌中的表达及临床意义

目的:探讨NF-кB和CXCR4在大肠腺癌发生、发展、浸润转移中的作用及临床意义.方法:应用免疫组织化学S-P法检测60例大肠腺癌、23例大肠管状腺瘤、25例正常大肠黏膜组织中NF-кB和CXCR4的蛋白表达情况.结果:大肠腺癌组中的阳性表达均高于大肠管状腺瘤组和正常大肠黏膜组,大肠管状腺瘤组中NF-кB和CXCR4的阳性表达均高于正常大肠黏膜组,且大肠腺癌组、大肠管状腺瘤组、正常大肠黏膜组中的阳性表达差异均有统计学意义(P均<0.05);大肠腺癌组中NF-кB和CXCR4的阳性表达均与癌组织的浸润深度、有无淋巴结转移有关(P均<0.05),与患者的性别、年龄及癌组织的分化程度无关(P均>0.05);大肠腺癌组织中NF-кB和CXCR4的表达呈正相关(r=0.346,P=0.007).结论:NF-кB和CXCR4的高表达与大肠腺癌的发生、发展、浸润转移密切相关.     

Expression of Bcl-2 and c-ErbB-2 in colorectal neoplasia

Several studies have been demonstrated the value of c-ErbB-2 and Bcl-2 in predicting the biological behaviour of tumors. The aim of this study was to investigate Bcl-2 and c-ErbB-2 expression in colorectal carcinomas and the correlation between their presence and other clinicopathologic parameters. Eighty-six colorectal carcinomas and 17 adenomas were stained with Bcl-2 and c-ErbB-2 immunohistochemically. Staining patterns were assessed semi-quantitatively and correlated with tumor size, Duke s classification, tumor differentiation, mucinous characteristic and anatomic locations. We detected Bcl-2 expression in 10 of 17 adenomas (58.8 %) and 31 of 86 carcinomas (36.04 %). Positive staining in normal mucosa was observed only in the compartment of cryptic cells. However neither the difference in the rates of Bcl-2 positivity in adenoma and carcinoma groups, nor the correlation with other mentioned clinicopathological parameters, were found statistically significant. Bcl-2 expression was found to be significantly high in mucinous carcinomas. Expression of c-ErbB-2 was observed in 12 of 86 (13.95 %) carcinomas. It was not detected in adenomas and normal mucosa. Although the incidence of c-ErbB-2 in nonmucinous carcinoma was higher than that of mucinous carcinoma, this was not significant. In addition we were unable to show any significant relation between c-ErbB-2 expression and other clinicopathologic features. Our result suggest that c-ErbB-2 protein expression in colorectal carcinomas, is not very frequent event. There is no correlation between c-ErbB-2 expression and malignant potential of colorectal carcinomas. Higher expressions of Bcl-2 in adenomas than carcinomas suggest us a possible role of Bcl-2 in early carcinogenesis of colon. However since we were unable to find any significant correlation between Bcl-2 expression and other parameters the impact of this gene on biological behavior is still unclear for us.     

大肠癌COX-2与MMP-2的基因表达及其意义

目的探讨COX_2和MMP_2蛋白在大肠癌组织中的表达、相互关系及意义。方法采用链菌素亲生物素-过氧化物酶法(S_P法)检测68例大肠癌及20例正常大肠组织COX_2、MMP_2蛋白的表达情况。结果大肠癌组织COX_2的阳性表达为79.4%(54/68),正常大肠组织COX_2的阳性表达为10.0%(2/20),COX_2在大肠癌组织中的阳性表达显著高于正常组织(P<0.01);大肠癌组织中MMP_2的阳性表达为73.5%(50/68),正常大肠组织MMP_2阳性表达为20.0%(4/20),MMP_2在大肠癌组织中的阳性表达显著高于正常组织(P<0.01);在大肠癌组织中COX_2、MMP_2蛋白的表达之间存在显著正相关(γ=0.627,P<0.01)。结论大肠癌组织中存在COX_2、MMP_2的高表达,且两者之间的表达强度具有等级相关性。COX_2蛋白可通过诱导MMP_2蛋白的表达上调,增加大肠癌细胞的侵袭力,从而成为其促进大肠癌浸润、转移的途径之一。     

IGFBP-2与结直肠癌的研究进展

胰岛素样生长因子结合蛋白2（IGFBP-2）是胰岛素样生长因子系统的一员,IGFBP-2在神经胶质瘤、结直肠癌、前列腺癌、卵巢癌、乳腺癌等多种恶性肿瘤细胞株、患者血清及其肿瘤组织中均有表达或表达水平升高。大量的临床实验及流行病学研究涉及了IGFBP-2与结直肠癌之间的关系,全文就目前IGFBP-2与结直肠癌的研究进展作一综述。     

COX-2及其抑制剂与结直肠肿瘤

动物实验、细胞学研究、流行病学研究和临床资料皆证明COX 2抑制剂可以在早期阶段阻止结直肠癌的发生。就COX 2理化特征、表达与调控、与结直肠癌的关系及其可能的机制予以综述     

Expression of ABCG2 and its Significance in Colorectal Cancer

The expression of ABCG2 in colorectal cancer and its relationship with invasion and metastasis is still not clear. In our study, immunohistochemical staining of ABCG2 was therefore performed for 60 cases of primary colorectal cancer. ABCG2 positive cancer cells were found to be mainly positioned in the front of carcinomatous tissue or between carcinomatous and non-carcinomatous margin tissues. In carcinomatous tissues and non-carcinomatous margin tissues, high expression rates forABCG2 were 36.7% (22/60) and 3.3% (2/60) respectively, with significant difference (χ2=5773.3, P<0.001). The rates of high expression of ABCG2 were 30% (9/30) and 6.7% (2/30) in 30 cases with and without positive lymph nodes, respectively. (χ2=5.45, P<0.025). From the present results expression of ABCG2 may be important in the progression and metastasis of colorectal cancer.     

Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer

The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer.After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice.From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector.When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control).This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer.     

Expression of Cyclooxygenase-2 (COX-2) in Colorectal Adenocarcinoma: an Immunohistochemical and Histopathological Study

Background: The aim of this study was to evaluate cyclooxygenase-2 (COX-2) immunoreactivity in colorectal adenocarcinomas and to find correlations with different pathological features. Materials and Methods: This study included 35 cases of colorectal carcinoma foir which surgical colectomy specimens were collected. Immunohistochemical staining of COX-2 (cyclooxygenase-2) is done by using the Streptavidin-biotin technique. Results: This work reveals that COX-2 is positive in most cases of colorectal carcinoma and negative in normal colon tissue with statistically non significant relations between COX-2 immunostaining and different pathological features. Conclusions: Our data suggest over expression of COX-2 protein in colorectal carcinoma in contrast to normal mucosa, with a possible role in cell proliferation in carcinogenesis.     

结直肠癌组织中RUNX3和hMSH2的研究进展

结直肠癌的发生、发展需要受多种基因的调控,人类RUNT相关转录因子3（RUNX3）和人类错配修复基因（hMSH2）在结直肠癌的发生、发展、转移及预后中起重要作用。RUNX3基因启动子区改变导致RUNX3基因转录表达下调及失活,参与多种肿瘤的发生、发展。hMSH2是错配修复基因,hMSH2蛋白在错配修复过程中通过核苷酸聚合酶而起作用,它失活后可引起复制错误的累积,从而引起突变表型,为探讨对RUNX3和hMSH2的一些相关性研究结直肠癌发生、发展机制奠定了基础。本文就国内外对RUNX3、hMSH2表达的异常与结直肠癌发生发展关系的研究进展作一综述。     

Genotype Frequencies of Cyclooxygenase 2 (COX2) Rare Polymorphisms for Japanese with and without Colorectal Cancer

Cyclooxygenase 2 (COX2) is an inducible enzyme synthesizing prostaglandins from arachidonic acid, which isthought to play an important role in colorectal carcinogenesis. Since the COX2 polymorphisms, if functional, maymodify the carcinogenesis pathway, the associations between the reported polymorphisms and colorectal cancer riskwere examined in a hospital-based case-control study. Six polymorphisms of the gene encoding COX2 were genotypedfor 241 non-cancer individuals (controls) and 148 colorectal cancer patients (74 colon cancer, 73 rectal cancer, and 1colorectal cancer date, 22 polymorphisms including the above six have been reported for COX2. The otherpolymorphisms remain to be examined.     

环氧合酶-2在大肠癌中的表达及其与预后的关系

目的探讨环氧合酶-2（COX-2）在大肠癌组织中的表达及其意义。方法应用S-P免疫组化法检测40例大肠癌石蜡标本、20例正常大肠组织中COX-2的表达。结果COX-2蛋自在40例大肠癌组织中高表达（36／40）,阳性表达率90％,与正常大肠组织相比差异非常显著（P〈0．01）;COX．2蛋白的表达与Dukes分期呈正相关（P〈0．05）;淋巴结有转移组与无转移组也有显著性差异（P〈0．05）。结论COX-2在大肠癌的发生及转移过程中发挥着重要作用,可作为预测大肠癌预后的临床指标之一。