沙眼衣原体、解脲支原体、淋病奈瑟氏菌感染状况分析

目的　探讨本地STD主要病原体的感染状况。方法　采用PCR方法检测我院门诊病人尿道和生殖道标本中沙眼衣原体 (CT)、解脲支原体 (UU)、淋病奈瑟氏菌 (Ng)。结果　男性患者CT、UU和Ng分别为 8 4 % ,9 0 %和2 2 0 % ,女性患者CT、UU和Ng感染率感染率分别为 8 2 % ,17 0 %和 8 3%。结论　本地区男女的STD主要病原体的感染谱不同 ,男性以Ng感染为主 ,女性以UU感染为主 ,UU已取代Ng成为女性STD首要病原体。     

男性淋病患者泌尿生殖道衣原体感染的调查

目的　调查男性淋病患者不同感染时期其合并沙眼衣原体感染的状况 ,为性传播疾病的预防、控制提供参考依据。方法　应用聚合酶链反应技术检测了 34 9例男性淋病患者及 5 3例健康男性 (对照组 )。结果　病例组和对照组衣原体感染率分别为 2 8 1% ,3 8% (P <0 .0 0 1)。处于不同时期的男性淋病患者衣原体感染率分别为 31 3% ,2 6 3% ,2 0 0 %(P >0 .0 5 )。结论　男性淋病患者不同的病程时期均可以合并沙眼衣原体感染且有较高的感染率 ;淋病患者是沙眼衣原体感染的危险因素之一     

实时荧光定量PCR法在检测泌尿生殖道感染病原体中的应用

目的评价荧光定量PCR（FQ-PCR）在实验室检测泌尿生殖道病原体、淋病奈瑟菌（NG）、解脲脲原体（UU）和沙眼衣原体（CT）中的应用。方法 464例拟诊为泌尿生殖道感染者的临床标本,用培养法检测NG、UU病原体和胶体金免疫层析法检测CT抗原,对同一标本同时用FQ-PCR检测NG、UU或CT的核酸。结果 FQ-PCR和培养法检出NG阳性率分别为28.4%（63/222）和14.9%（33/222）,P〈0.01,检测UU的阳性率分别为41.7%（126/302）和32.5%（98/302）,P〈0.05,FQ-PCR和金标法检测CT阳性率分别为18.2%（57/314）和6.1%（19/314）,P〈0.001,FQ-PCR对3种病原体的检出率均明显高于培养法或金标法。结论 FQ-PCR法检测NG、CT以及UU在临床诊断NG,UU和CT感染中具有重要应用价值,是常规方法的有效补充。     

PCR联合检测54例泌尿生殖道感染三种病原体的报告

淋球菌(NG),沙眼衣原体(Ct)和解脲支原体(Uu)混合感染越来越引起人们的重视。我们利用PCR对本所性病门诊1997-06～1998-03就诊54例泌尿生殖道感染患者同时进行了三种病原体联合检测,现报告如下。     

男性不育症与沙眼衣原体、解脲支原体感染（附92例分析）

目的：探讨男性不育与沙眼衣原体、解脲支原体感染的关系。方法：支原体分离培养法和衣原体胶体金法对巴州农村地区男性不育症患者进行沙眼衣原体、解脲支原体检测,分男性精液异常、性腺慢性炎症引起的不育组、与其他因素引起的不育组,进行沙眼衣原体、解脲支原体检测结果的比较。结果：男性沙眼衣原体、解脲支原体阳性检出率分别为46.56%40.22%。男性生殖道感染组沙眼衣原体、解脲支原体阳性检出率高于非感染因素组沙眼衣原体、解脲支原体阳性检出率,两组存在显著性差异（P〈0.05）。结论：沙眼衣原体和解脲支原体引起的男性生殖道感染、精液异常是造成不育的因素之一。     

女性尖锐湿疣合并其他性传播疾病124例的实验室检测

目的：探讨女性尖锐湿疣合并其他性传播疾病感染的发病率及流行情况。方法：对确诊尖锐湿疣的女性患者,进行淋病、非淋菌性宫颈炎、念珠菌病、梅毒等性传播疾病的实验室检测。结果：在124例入选病例中,合并感染57例,占45.97%。其中梅毒4例（3.23%）,沙眼衣原体12例（9.68%）,解脲脲原体38例（30.65%）,人型支原体18（14.52%）,淋球菌13例（10.48%）及念珠菌病13例（10.48%）,合并1种病原体感染26例（20.97%）,合并2种病原体感染19例（15.32%）,合并3种及3种以上病原体感染12例（9.68%）。HIV0例。结论：尖锐湿疣易合并其他性传播疾病感染,其他性传播疾病是引起尖锐湿疣的易感因素。尖锐湿疣患者应全面进行其他性病实验室项目及HIV抗体的检查。     

部分性病病原体在重庆地区流行情况分析

为了解淋球菌(NG)、解脲支原体(UU)、沙眼衣原体(CT)等性病病原体在重庆地区的感染率及在人群中的分布情况,随机收集我院2000-08～2001-04的门诊标本,采用荧光定量PCR方法检测上述病原体,并对这三种病原体的感染率以及感染者的性别差异、年龄差异分析如下:     

聚合酶链反应在诊断常见性传播疾病病原体中的应用

性传播疾病(sexually transmitted dis-ease,STD)是妇科门诊常见的疾病,近年来STD的报道有所增加,有关从临床检验学方面的报道也有增加。在西方国家,沙眼衣原体(chlanydia trachomatis,CT)已成为STD中最常见的一种病原体,淋病奈瑟菌(Neis-seria gonorrhoeae,NG)仅次于CT,而在中国,NG仍是STD最常见的一种病原体,但CT的感染呈迅速上升趋势。在STD治疗中,对病原体的明确断显得尤为重要,而在既往检测STD病原体的方法中均有明显不足。我们应用聚合酶链反应(Polymerasechairr reaction,PCR)检测190例STD病原体。现将结果报道如下。     

PCR检测淋病奈瑟氏菌、沙眼衣原体、解脲支原体、人乳头瘤病毒的核酸

1997-01～1998-07我院门诊对1435例泌尿生殖系统感染的尿道及阴道分泌物和慢性前列腺炎的前列腺液用PCR检测淋病奈瑟氏菌(NGH)、沙眼衣原体(CT)、解脲支原体(UU)及人类乳头瘤病毒(HPV)等四种病原体的核酸,结果报告如下。 材料与方法 ①研究对象:本组1435例病人为我院皮肤科、妇产科和泌尿外科就诊的泌尿生殖系统感染病例,其中男性427例,女性1008例。②标本采集:用加有无菌生理盐水之无菌有盖塑料离心管,     

纳米金新型基因芯片检测系统结合限制性酶切非PCR法同时检测多种病原性微生物的研究

目的 研究将纳米金新型基因芯片检测系统与限制性酶切非PCR法结合以实现同时对多种病原性微生物进行检测的方法.方法 以幽门螺杆菌、肺炎支原体、沙眼衣原体、白色念珠菌、解脲脲原体、EB病毒作为检测目标,选择Hha Ⅰ内切酶作为工具酶.对所有待检样本核酸进行Hha Ⅰ酶切,对酶切产物进行同聚A加尾处理,并结合纳米金新型芯片检测系统,分别与特异性探针及通用探针进行两次杂交以完成对目标微生物的检测.通过对168份临床样品的检测,考察新构建芯片的检测结果与荧光定量PCR检测结果的符合率;对构建芯片的稳定性及检测灵敏度进行测试.结果 新构建的基于限制性酶切非PCR法的纳米金芯片技术实现了对选定目标的检测.对168份临床样品的检测结果显示,该芯片检测与荧光定量PCR检测的结果有150例完全相符,符合率为89.2%;进一步芯片检测性能实验结果表明,该芯片检测稳定性为100%,检测敏感性为50pmol/L.结论 本研究构建的基于限制性酶切非PCR法的纳米金芯片技术可以同时实现对细菌、真菌、支原体、衣原体及病毒等微生物的检测.适用性广且对大幅度提高检测通量有积极意义.     

New Oily Agents for Targeting Chemoembolization for Hepatocellular Carcinoma

Purpose: The evaluation of new oily agents for targeting chemoembolization for hepatocellular carcinoma. Methods: Five types of oily preparation were injected into the hepatic artery of 54 rabbits inoculated with VX2 carcinoma cells in order to evaluate (1) the safety of these preparations, (2) their histologic distribution and the amount of agents remaining at tumor sites, and (3) computed tomographic (CT) images obtained. Of these preparations, three were made by mixing non-iodinated poppy seed oil and a thickener and then adjusted to have a viscosity lower than, equal to, or higher than that of lipiodol. A fourth preparation was a mixture of lipiodol and a thickener with a higher viscosity than lipiodol alone, and the fifth preparation was lipiodol alone. Results: (1) No injury to the hepatic parenchyma was observed hematologically or histologically. (2) With increase in the viscosity, a significantly larger amount of agent remained at the tumor site. No agent was present at normal sites 14 days after intraarterial injection, regardless of which preparation was given. (3) On CT scans following intraarterial injection, tumor cells were visibly deeply stained in the non-iodinated preparation groups, while the lipiodol groups were not evaluable because of excessively high attenuation. Conclusion: The non-iodinated oily preparations and highly viscous oily preparations developed in the present study were more useful than lipiodol for treatment of hepatic tumors.     

Novel Materials for Low-Contrast Phantoms for Computed Tomography

Acceptance testing and quality control of computed tomography (CT) scanners are of great importance. While most procedures and phantoms for testing other parameters are widely accepted, there is still discussion and uncertainty about low-contrast (LC) performance tests that measure the capability of a CT scanner to discriminate low-contrast objects. This work investigated the development of LC phantoms with available, low-cost polystyrene resin materials and some selected additives. We designed and tested phantoms with several different contrast steps by generating contrast in two different ways, one based on 'physical density difference' and the other on 'atomic number difference'. Physical density difference was achieved by adding a small amount of glycerin to the polystyrene resin, both having similar low atomic-number elements but differing in the density of their atoms. Atomic number difference was achieved by adding a small amount of iodobenzene to the resin, both having approximately the same physical density (less than 1% variation in density) but different atomic (i.e., elemental) composition. Prototypes were evaluated using a Philips Tomoscan LX system and varying beam properties. The behavior and validity of the results are discussed.     

Self-test software for PowerPoint: a tool for self-learning

RATIONALE AND OBJECTIVES: We developed self-test software to improve self-learning efficiency using Microsoft PowerPoint data files. CONCLUSION: This tool can be run on IBM-compatible computer under Microsoft Windows. It is a new useful and interactive tool for self-learning. This tool allows users to do view the cases in the PowerPoint data files by random or sequentially. Goal-oriented effective self-learning is possible from methods that conjecture the possible differential diagnosis without promptly seeing correct diagnosis. Thus effective and interactive self-learning is possible.