北虫草复合制剂对小鼠免疫功能的影响

探讨北虫草复合制剂对小鼠免疫功能的调节作用。建立小鼠免疫力降低的动物模型,实验分6组:对照组、衰老/免疫抑制模型组、白介素-2(IL-2)和北虫草复合制剂的不同剂量组。采用称重法测定免疫器官重量,计算胸腺指数和脾指数。小鼠溶血素抗体生成采用绵羊红细胞致敏法。北虫草复合制剂对小鼠脾脏指数和胸腺指数的影响:实验组(50.2±2.4与27.6±3.6)明显高于模型组(45.6±4.8与23.6±3.6),单位:(mg/10 g体重),差异有显著性(P<0.05)。对小鼠溶血素抗体生成的影响:实验组(53.53±7.8)高于药物对照组(36.50±7.3)。北虫草复合制剂能恢复衰老小鼠的胸腺指数和脾脏指数,增强免疫抑制小鼠血清溶血素含量,因此,对免疫功能低下的小鼠模型具有免疫调节作用。     

千斤拔多糖对小鼠免疫功能的调节作用

为研究千斤拔多糖对正常及免疫低下小鼠免疫功能的调节作用,该研究选用SPF级BALB/c小鼠,免疫抑制小鼠采用隔天皮下注射环磷酰胺(40 mg·kg~(-1))5次,通过测定脾脏、胸腺质量及计算脏器指数,采用碳粒廓清法计算单核巨噬细胞吞噬功能,在鸡红细胞免疫后测定小鼠血清溶血素抗体水平,同时观察高、低剂量千斤拔多糖(剂量分别为500、1 000 kg·d~(-1))对正常小鼠及免疫低下小鼠免疫调节的影响。结果表明:千斤拔多糖高、低剂量组对正常及免疫低下小鼠均有不同程度的增加脾脏指数、胸腺指数的作用;千斤拔多糖高、低剂量组对正常及免疫低下小鼠均能提高廓清指数,但没有统计学意义;千斤拔多糖高剂量组对正常及免疫低下小鼠的血清溶血素抗体水平都有显著性提高。这说明千斤拔多糖能有效提高正常及免疫低下小鼠免疫功能的作用。     

非洲乍得湖钝顶螺旋藻多糖对S_(180)腹水瘤小鼠免疫抗肿瘤作用的实验研究

采用小鼠S180腹水瘤模型,观察非洲乍得湖钝顶螺旋藻多糖不同剂量组小鼠的生存状况、瘤体外观,测定非洲乍得湖钝顶螺旋藻多糖对小鼠免疫器官指数及血清IL-2和TNF-α水平的影响,以探讨非洲乍得湖钝顶螺旋藻多糖对肿瘤的抑制作用及其对S180腹水瘤小鼠的免疫调节作用。结果表明:高、中、低三个剂量的非洲乍得湖钝顶螺旋藻多糖均能改善腹水瘤小鼠的生存状况,缩小肿瘤在体内扩散的范围;不同浓度的非洲乍得湖钝顶螺旋藻多糖组小鼠胸腺和脾脏指数均高于模型组,肝体/比均低于模型组,以中剂量组作用效果最为显著(P0.05);不同剂量的非洲乍得湖钝顶螺旋藻多糖均显著提高小鼠血清TNF-α的水平(P0.05),对IL-2含量的影响表现为三个剂量组的均高于模型组,且高、中剂量组的与模型组相比差异显著(P0.05);三种剂量组之间相比,中剂量组对IL-2和TNF-α的诱生作用最明显,与高、低剂量组相比差异均显著(P0.05)。研究结果提示,非洲乍得湖钝顶螺旋藻多糖能够促进小鼠免疫器官的生长发育从而增强免疫功能,也可通过促进小鼠免疫细胞分泌TNF-α和IL-2而发挥免疫调节作用。     

马衔山黄芪水提物抗肿瘤转移及生长初步研究

目的:研究马衔山黄芪对转移性肝癌的抑制及其生长影响。方法:经尾静脉接种H22肝癌细胞悬液建造肿瘤转移小鼠模型后,随机分为6组。1 d后,分别以生理盐水,环磷酰胺,低、中、高剂量马衔山黄芪水提物以及马衔山黄芪水提物合并环磷酰胺处理20 d后,处死小鼠测定体重、胸腺及脾脏重量以及巨噬细胞吞噬率,观察小鼠肺部转移瘤生长情况。结果:马衔山黄芪低、中、高剂量组以及马衔山黄芪合并环磷酰胺(CTX)组的瘤结节数少于N.S组,马衔山黄芪中、高剂量组的胸腺、脾脏指数,腹腔巨噬细胞吞噬率高于N.S组。结论:马衔山黄芪具有免疫调节功能,对肿瘤转移和生长有抑制作用。     

胶原蛋白肽增强小鼠免疫力的实验研究

目的 探讨用生物酶提取的胶原蛋白肽增强小鼠免疫力的作用及相关机制.方法 80只清洁级昆明小鼠被随机分成正常对照组、胶原蛋白肽低剂量组[3 g/(kg·bw)]、中剂量组[7.5 g/(kg·bw)]和高剂量组[12 g/(kg·bw)],每日给药2次,连续2周.检测免疫器官重量、抗体生成细胞数(Jerne改良玻片法)、半数溶血值(HC50)的测定及小鼠腹腔巨噬细胞吞噬鸡红细胞实验.结果 高剂量组可提高脾脏指数、胸腺指数、半数溶血值及吞噬细胞吞噬率和吞噬指数、可增加抗体生成细胞数;中剂量组可提高胸腺指数、增加抗体生成细胞数、提高小鼠半数溶血值及吞噬细胞吞噬率和吞噬指数;低剂量组可增加抗体生成细胞数、提高吞噬细胞的吞噬率和吞噬指数.结论 三种剂量胶原蛋白肽对正常小鼠的细胞免疫、体液免疫均有显著的增强作用,高剂量组效果最强.     

雪莲培养物对小鼠免疫功能的影响

考察雪莲细胞培养物对小鼠免疫功能的影响,对比了不同剂量组3.4、16.7、33.4mg/kg.d的雪莲培养物喂小鼠后,对测定指标的影响,结果显示,三个剂量组对脾脏指数无影响;剂量组3.4 mg/kg.d能提高巨噬细胞吞噬率和T淋巴细胞转化率;中、高剂量组对血清溶血素水平、巨噬细胞吞噬率,T淋巴细胞转化率等四项免疫指标有显著(p0.05)或极显著(p0.01)增高。说明雪莲培养物具有良好的增强免疫力的作用。     

参芝发酵产物的抗肿瘤活性及其对小鼠免疫功能的影响

为了探索红参水煎液的灵芝发酵产物对H22荷瘤小鼠的抗肿瘤活性及其对小鼠免疫功能的影响,通过体内抗肿瘤实验和增强免疫功能实验从抑瘤率、对免疫器官的影响指数、对非特异性免疫、体液免疫及细胞免疫的影响5个方面对该产物做了功能性评价。结果表明,在抗肿瘤实验中,参芝发酵产物高剂量组的抑瘤率达到51.65%,脾指数和胸腺指数均高于对照组和环磷酰胺(CTX)组;增强免疫功能实验中,3个实验的给药组小鼠和对照组小鼠相比都有显著性差异(P<0.01)。由此可见,将灵芝与人参在发酵层次上配伍具有显著的抑制荷瘤小鼠肿瘤生长及增强小鼠免疫功能的作用。     

复合益生菌粉对小鼠免疫机能的影响研究

目的：研究复合益生菌粉在增强免疫力功能方面的作用。方法：采用经口灌胃给予方式,设受试物组3组,分别为低、中、高剂量组,同时设阴性对照组（生理盐水）,从小鼠体重指标、细胞免疫、体液免疫、单核—吞噬细胞活性、NK细胞活性等方面综合评价复合益生菌对小鼠体内免疫机能的影响。结果：与空白对照组相比,中、高剂量下的益生菌粉组中的小鼠抗体生成细胞数显著增加（P<0.05）;低、中、高剂量益生菌组中的小鼠碳廓清功能显著提高（P<0.05）;高剂量组的NK细胞活性比对照组有显著性提高（P<0.01）。不同剂量的益生菌给予期间,均未见益生菌粉对小鼠体重、脾脏/体重比值、胸腺/体重比值、足趾肿胀度、淋巴细胞增殖能力、半数溶血、巨噬细胞吞噬鸡红细胞能力有影响。结论：该复合益生菌粉能够增强体液免疫、单核—吞噬细胞活性和NK细胞的活性,具有一定的免疫增强功能。     

蜜蜂巢脾提取物对小鼠免疫调节作用的影响

本研究旨在从体重、脏器/体重比值、体液免疫、细胞免疫和NK细胞活性等研究蜜蜂巢脾提取物对小鼠免疫调节作用的影响。采用160只雄性昆明种小鼠为研究对象,按体重随机分4批,每批4个组别,分别为阴性对照组、蜜蜂巢脾提取物高、中、低剂量组,测定免疫学指标。本研究结果显示,小鼠体重增长正常,各组小鼠脏器/体重比值、胸腺/体重比值无明显变化,均无显著性差异。高、中剂量组的小鼠脾淋巴细胞转化和血清溶血素高于阴性对照组且有显著性差异(P0.05),三个剂量组比阴性对照组的校正廓清指数α和NK细胞活性有显著差异(P0.05)。由此可见,蜜蜂巢脾提取物能显著提高小鼠的体液免疫和细胞免疫能力及抗氧化活性。     

蛋白质粉对小鼠免疫功能的影响

目的探讨蛋白质粉对正常小鼠免疫调节作用。方法将BALB/c小鼠随机分为3批,每批分为4组,分别进行了小鼠免疫器官/体质量比值测定和小鼠碳廓清实验;绵羊红细胞诱导小鼠DTH、抗体生成细胞检测和血清凝血素测定（HC50）;ConA诱导的小鼠脾淋巴细胞转化实验和乳酸锂脱氢酶法（LDH）测定NK细胞活性;小鼠腹腔巨噬细胞吞噬鸡红细胞实验。结果10.00 g/kg剂量的蛋白质粉可增强绵羊红细胞诱导小鼠DTH能力（P〈0.05）,促进抗体生成细胞数的生成（P〈0.01）。3.33 g/kg和10.00 g/kg剂量组能促进ConA诱导的小鼠脾淋巴细胞转化能力（P〈0.05或P〈0.01）和血清凝血素的生成（P〈0.05）;三个剂量组均能提高小鼠腹腔巨噬细胞吞噬鸡红细胞能力（P〈0.05或P〈0.01）;3.33 g/kg和10.00 g/kg剂量组能提高NK细胞活性（P〈0.05）;但对小鼠碳廓清能力和免疫器官/体重比值无明显影响。结论蛋白质粉对正常小鼠的细胞、体液免疫和单核-巨噬细胞功能和NK功能有促进作用,即具有增强免疫力功能。     

中国被毛孢和蝙蝠蛾拟青霉小鼠免疫功能调节作用的比较

目的观察中国被毛孢和蝙蝠蛾拟青霉对小鼠免疫功能的影响。方法分别给予中国被毛孢和蝙蝠蛾拟青霉后,测定观察对小鼠碳粒廓清功能、迟发性变态反应、溶血素抗体、淋巴细胞增殖和NK细胞活性等的影响。结果3．0g／kg中国被毛孢和蝙蝠蛾拟青霉可显著提高小鼠吞噬指数和半数溶血值（P〈0．01）、显著提高T、B淋巴细胞增殖能力（P〈0．01,P〈0．05）、显著提高NK细胞活性（P〈0．05）,0．5～1．5g／kg中国被毛孢都能显著抑制小鼠耳廓肿胀（P〈0．01,P〈0．05）,同时还能抑制脾脏和胸腺增大（P〈0．01,P〈0．05）。结论中国被毛孢和蝙蝠蛾拟青霉均具有免疫调节作用,而中国被毛孢免疫抑制及增强天然免疫系统作用要优于蝙蝠蛾拟青霉,而对适应性免疫系统增强作用蝙蝠蛾拟青霉优于中国被毛孢。     

冬虫夏草菌丝提取物对化学性肝损伤的辅助保护作用

为明确冬虫夏草菌丝提取物对急性肝损伤小鼠谷丙转氨酶（ALT）、谷草转氨酶（AST）、肝细胞变性及坏死程度的影响,采用四氯化碳（CCl4）诱导小鼠急性化学性肝损伤模型,将动物随机分成5组,分别是空白对照组、模型组、冬虫夏草菌丝提取物低剂量组（1.11g/kg BW）、中剂量组（3.33g/kg BW）、高剂量组（10.00g/kg BW）,检测血清ALT、AST值,并取肝脏作病理切片,观察肝脏的病理损伤情况。冬虫夏草菌丝提取物高剂量组能明显降低CCl4急性肝损伤小鼠血清ALT值,减轻肝细胞坏死程度,表明冬虫夏草菌丝提取物对化学性肝损伤有辅助保护功能。     

当归内酯对免疫抑制小鼠免疫功能的重建作用(英文)

探讨当归内酯(ASDL)对免疫抑制小鼠免疫功能的重构作用。通过小鼠腹腔注射环磷酰胺建立免疫抑制动物模型。采用免疫器官重量法和小鼠腹腔巨噬细胞吞噬鸡红细胞实验检测了ASDL对非特异性免疫功能的影响;用血清溶血素分光光度法检测了对体液免疫功能的作用;用MTT法进行了致分裂原诱导的小鼠脾淋巴细胞的增值反应实验,再用乳酸脱氢酶法测定了NK和CTL细胞活性,从而确定ASDL对小鼠细胞免疫功能的影响。结果表明:ASDL能够对免疫低下小鼠的非特性和特异性免疫功能起到一定的重构作用。但是这种效果并不是剂量依赖性的,20 mg/kg这个剂量的效果明显好于5和80 mg/kg这两个剂量。上述结果表明ASDL能够显著提高免疫低下小鼠的免疫功能。     

裂盖马鞍菌提取物体内抗氧化活性研究

以新疆特色食用菌裂盖马鞍菌为材料,研究裂盖马鞍菌乙醇提取物、乙酸乙酯、氯仿、石油醚萃取物对小鼠体内的抗氧化活性,分高（2g/kg）、中（1g／kg）、低（0．2g／kg）三个剂量对小鼠灌胃给药测定小鼠肝脏和血清中丙二醛（MDA）含量和超氧化物岐化酶（SOD）活力。结果表明,与阴性对照（生理盐水）相比裂盖马鞍菌提取物能明显提高小鼠肝脏和血清中SOD的活力,降低MDA的含量（P〈0．01）;与阳性对照维生素E（VE）（1g/kg）相比乙醇提取物、其他溶剂萃取物高浓度（2g／kg）作用极显著（P〈0．01）;与维生素C（VC,1g／kg）相比乙醇提取物高浓度（2g/kg）作用极显著（P〈0．01）,提示裂盖马鞍菌提取物具有抗氧化活性。     

Effect of dose schedule of vitamin E and hydroxethylruticide on intestinal toxicity induced by adriamycin

CDF1 mice receiving Adriamycin, 12 mg/kg IP develop a toxic GI mucositis. The mean survival in CDF1 mice after Adriamycin injection was found to be 6.5 +/- 2.0 weeks and could be increased by alcohol or acetate Vitamin E pretreatment (with 2 g/kg qDx7d) to 22.06 +/- 12.3 weeks or by treatment with Venoruton after Adriamycin (qDx7 with 1.5 g/kg) to 23.7 +/- 12.7 weeks. Other schedules were ineffective or harmful. The ability of Venoruton to enhance survival when given after Adriamycin encouraged us to proceed to tumor bearing mice. The maximum survival with CDF1 mice bearing 5 X 10(6) L1210 cells was 1 +/- 0.2 week which could be increased to 2.17 +/- 0.8 weeks with optimal dose Adriamycin (10 mg/kg). Optimum survival with Venoruton and a single dose of Adriamycin was 2.45 +/- 0.91 weeks with Venoruton, 1.5 g, qd X 14, and 12 mg/kg Adriamycin. Treatment of L1210 bearing mice with Adriamycin, 10 mg/kg on days 1 and 8, yielded a survival of 2.23 +/- 0.7 weeks. An equitoxic regimen of Adriamycin, 11 mg/kg on days 1 and 9, plus Venoruton, 1.5 g, qd X 14, increased survival 30% to 3.08 +/- 2.9 weeks. Venoruton is a promising agent to increase the therapeutic index of Adriamycin.     

Immune responses in mice to arecoline mediated by lymphocyte muscarinic acetylcholine receptor

There is evidence that lymphocytes possess all the components of the cholinergic system independent of neuronal innervations. Thus, potential therapeutic applications of drugs targeting the neuronal cholinergic system might have side effects on the immune system. This study investigated whether arecoline could affect immunological functions in mice and explored the mechanism of the effect of arecoline on the immune system. To investigate this, arecoline at the dose of 2mg/kg was administered subcutaneously in BALB/c mice for 4 weeks to evaluate changes in immunological function both in vivo and in vitro. Several indices were used to assess immunological activation, including the spleen index, serum hemolysin levels, interleukin (IL)-2 and splenocyte proliferation. Our results showed a significant reduction in treated animals with respect to the control group in the following tests: the spleen index (86%), hemolysin against sheep red blood cells (68%), IL-2 production (73%), and splenocyte proliferation induced by concanavalin A or lipopolysaccharide (76% and 74%, respectively). The muscarinic receptor antagonist atropine (1mg/kg) reversed the inhibition of the four immune-related parameters mentioned above. Chronic atropine alone did not significantly affect the immune response. To our knowledge, this is the first study to demonstrate that arecoline interferes with the immune system by targeting the muscarinic acetylcholine receptors of the non-neuronal cholinergic system.     

益寿汤对小鼠免疫和抗应激能力的影响

给小鼠益寿汤１ｇ／ｋｇ,２ｇ／ｋｇ,４ｇ／ｋｇ灌胃连续７ｄ,分别检测其抗应激能力和免疫功能的变化。结果表明：益寿汤４ｇ／ｋｇ组小鼠较对照组负重游泳耗竭时间和常压耐缺氧生存时间明显延长,益寿汤２ｇ／ｋｇ和４ｇ／ｋｇ可提高小鼠耐寒能力,各剂量益寿汤均可显著提高小鼠血清溶血素及碳粒廓清吞噬数,但对迟发型变态反应无明显影响。     

枸杞多糖抗氧化作用的研究

目的:研究枸杞粗多糖(Lycium Barbarum Polysaccharide,LBP)在小鼠体内的抗氧化作用。方法:用高(400mg/kg.d)、中(200mg/kg.d)、低(100mg/kg.d)剂量的枸杞粗多糖生理盐水溶液对D-半乳糖(100mg/kg.d)之衰老模型小鼠和正常小鼠灌胃。结果:枸杞粗多糖能较显著(P0.01)提高小鼠血清、肝脏及脑组织中SOD活性,降低MDA含量;极显著(P0.01)提高正常小鼠常压耐缺氧能力和游泳抗疲劳能力;此外小鼠的脾指数和胸腺指数均得到显著提高,表明枸杞粗多糖对提高小鼠的机体免疫水平具有重要的促进作用。结论:枸杞粗多糖对小鼠具有显著的抗氧化、抗衰老作用。     

The effects of vitamin E on antiacid stress ability in juvenile soft-shelled turtles (Pelodiscus sinensis)

We determined the effect of dietary supplementation with vitamin E (0-, 50-, 250-, 500-, 1000- and 5000-mg/kg diet for 4 weeks) on antistress ability in juvenile soft-shelled turtle (Pelodiscus sinensis). Half of the turtles per dose group were treated by acid stress for 24 h. The results showed that phagocytosis of blood cells in the control group significantly decreased after acid stress while the other five groups had no significant changes compared with those of before stress. Serum bacteriolytic activity in the control group and the group supplemented with 50-mg vitamin E/kg diet significantly decreased after acid stress. The other four groups showed no significant differences compared with those before stress. Serum bactericidal activities in all groups notably decreased after acid stress, but the difference of serum bactericidal activity in before and after stress had a decreased tendency from the control group to the highest dose group. Serum cortisol levels in the control group were significantly increased while the other five groups had no notable increases after acid stress. Liver vitamin E levels in all groups had no notable changes compared with those before stress but there was a tendency to decrease after acid stress. These results suggest that acid stress depress immune function and increase serum cortisol levels in turtles while vitamin E alleviate the adverse effects caused by acid stress.     

Activation of macrophages and the intestinal immune system by an orally administered decoction from cultured mycelia of Cordyceps sinensis

The effects of an orally administered hot-water extract (HW) from cultured mycelia of Cordyceps sinensis on the activation of macrophages and the intestinal immune system were studied in mice. The general composition of HW was 83.9% carbohydrate, 11.8% protein, 1.9% lipid and 2.4% ash, and the carbohydrates were mainly composed of glucose, mannose, galactose and arabinose (molar ratio of 1.0:0.8:0.5:0.1). HW stimulated the activation (1.7-fold of the saline control) of macrophages and IL-6 production (1.5-fold) at 2.0 g/kg/day. Analyzing the culture supernatant of Peyer's patch cells from C3H/HeJ mice that had been fed with HW at 1.0 g/kg/day for 7 days indicated that the bone marrow cells had significantly proliferated (1.9-fold). In addition, the amounts of GM-CSF and IL-6 in the culture supernatant of Peyer's patch cells at the same dose were significantly increased (1.8-fold and 2.2-fold, respectively). These results indicate that an oral administration of HW may modulate IL-6 production by the activation of macrophages, and also enhance the secretion of hematopoietic growth factors such as GM-CSF and IL-6 from Peyer's patch cells. Since such cytokines as GM-CSF and IL-6 from Peyer's patch cells act on the systemic immune system, it can be assumed that orally administered HW modulated not only the local but also systemic immune system.