慢性乙型肝炎患者外周血CD4~+CD25~+Treg与CD4~+和CD8~+ T淋巴细胞亚群的相关研究

目的:探讨慢性乙型肝炎患者外周血中CD4+CD25+调节性T细胞的含量和CD4+CD8+T淋巴细胞亚群分布,两者之间相关性及与HBV的相关性。方法:采用流式细胞术检测50例慢性乙型肝炎患者和20例健康对照者外周血中CD4+CD25high、CD4+CD25+Foxp3+Treg细胞表达及CD3/CD4/CD8 T淋巴细胞亚群,荧光定量PCR法检测HBV DNA含量。结果:慢性乙型肝炎患者外周血中CD4+CD25highTreg明显高于健康对照组(P0.01),且随HBV DNA载量增加,患者外周血中CD4+CD25highTreg细胞的水平逐渐升高。慢性乙型肝炎患者外周血中CD4+CD25+Foxp3+Treg细胞也相应增高,且与CD4+CD25highTreg细胞的变化成正相关(r=0.890,P0.001)。与健康对照组比较,患者组CD4+T细胞百分率及CD4+/CD8+比值均降低,而CD3+T细胞和CD8+T细胞百分率差异无显著性(P0.05)。CD4+CD25highTreg细胞与HBV DNA取对数后成正相关(r=0.782,P0.001),与谷丙转氨酶(ALT)成正相关(r=0.432,P0.005);与CD3+、CD4+、CD8+T细胞水平及CD4+/CD8+比值均无相关性(P0.05)。CD3+、CD4+、CD8+T淋巴细胞及CD4+/CD8+比值与HBV DNA载量之间亦无相关性(P0.05)。结论:慢性乙型肝炎患者外周血中CD4+CD25+Treg细胞增高,且与HBV的复制水平及ALT增高具有一致性,而T细胞亚群是否可作为监测CHB患者免疫状态的指标需进一步探讨。     

慢性乙型肝炎病毒感染患者外周血CD8~+ T淋巴细胞表面淋巴细胞活化基因3水平升高

目的观察慢性乙型肝炎病毒(HBV)感染处于不同自然史患者外周血T淋巴细胞表面淋巴细胞活化基因3(LAG-3)表达的变化,探讨抑制性受体LAG-3在T细胞的表达变化与处于不同自然史慢性HBV感染者血清丙氨酸氨基转移酶(ALT)、乙型肝炎病毒(HBV)e抗原(HBe Ag)和HBV DNA水平的关系。方法选取处于慢性HBV感染自然史为免疫耐受期(IT)患者24例、免疫清除期(IC)32例和非活动或低复制期(LR)患者31例,健康体检者30例为正常对照(HC)。应用流式细胞术检测外周血CD8+T细胞和CD4+T细胞表达LAG-3的比例;免疫分析法、生化分析法和PCR分别检测患者肝脏功能、HBV血清学标志物和HBV DNA水平。采用Mann-Whitney U检验分析各组间T细胞LAG-3表达率的差异;Spearman等级相关性分析各期慢性HBV感染患者外周血T细胞LAG-3表达阳性率与血清ALT、HBV DNA和HBe Ag水平的相关性。结果慢性HBV感染者外周血LAG-3+CD8+T细胞的比例较HC组显著增高,且处于IT期的患者外周血LAG-3+CD8+T细胞的比例明显高于IC期或LR期患者及HC组。处于IC期患者外周血LAG-3+CD8+T细胞和LAG-3+CD4+T细胞比例与患者血清中ALT水平呈显著负相关;IT组患者外周血LAG-3+CD8+T细胞和LAG-3+CD4+T细胞比例与血清中HBe Ag呈正相关。然而,不同自然史慢性HBV感染患者外周血LAG-3+CD4+T细胞比例和LAG-3+CD8+T细胞比例与血清HBV DNA水平无相关性。结论 LAG-3在慢性HBV感染患者外周血CD8+T细胞高表达,尤其是IT期患者CD8+T细胞高表达,可能是造成CD8+T细胞功能低下、HBV在肝脏长期复制的重要因素之一。     

CD30在肾综合征出血热患者T细胞表达的检测及其临床意义

目的 :研究CD3 0 在肾综合征出血热 (HFRS)患者急性期外周血T淋巴细胞亚群的表达及意义。方法 :应用免疫荧光抗体双重标记和流式细胞仪技术分析CD3 0 在HFRS患者急性期外周血CD 4,CD 8T细胞表达的水平。结果 :CD 4CD-3 0 细胞在重型组 ,中轻型组和正常组三组间均无显著差异 (P >0 0 5 ) ;患者CD 4CD 3 0 ,CD 8CD-3 0 细胞明显增加 ,且重型组 ,中轻型组和正常组三组间均有极显著差异 (P <0 0 1)。结论 :HFRS患者急性期存在体液免疫和细胞免疫功能亢进 ,各种T淋巴细胞亚群的比例失衡与该病免疫发病机制及病情的严重程度密切相关。     

慢性乙型肝炎患者肝功能及血清HBeAg和HBV DNA载量与肝组织病理相关性的研究

目的 探讨慢性乙型肝炎患者肝功能、HBeAg及HBV DNA水平与肝组织病理炎症分级和纤维化分期的关系.方法 选择233例慢性乙型肝炎患者进行肝穿病理学检查,同时所有患者检测HBV DNA、HBeAg及肝功能,比较患者的肝功能、HBeAg及HBV DNA水平在不同病理炎症分级及纤维化分期中的差异情况.结果 不同的炎症分级患者中,ALT以C3组最高,G0～1组最低,各组间比较差异有统计学意义(P =0.016);TBil以G4组最高,G0～1组最低,各组间比较差异有统计学意义(P=0.000);HBV DNA载量各组间差异无统计学意义.不同的纤维化分期患者中,ALT各组间比较差异无统计学意义;TBil以S4组最高,S2组最低,各组间比较差异有统计学意义(P=0.039);HBV DNA载量各组间差异无统计学意义.炎症分级为G3～4的患者比例在HBeAg阳性组与阴性组差异无统计学意义.纤维化分期S3～4的患者比例在HBeAS阳性组(38%)比HBeAg阴性组(53%)低,两组差异有统计学意义(P=0.025).结论 慢性乙型肝炎患者血清HBV DNA水平的高低不能反映其肝脏炎症及纤维化程度,HBeAg阴性慢乙肝患者肝组织纤维化程度较高,TBil水平与肝组织炎症分级及纤维化分期均有良好的相关性,ALT水平与炎症分级有一定的关联性,但与纤维化分期无关.     

乙型肝炎患者免疫功能的检测及其临床意义

研究乙型肝炎患者外周血T淋巴细胞亚群、NK细胞和血清免疫球蛋白的变化及临床意义.采用流式细胞仪检测150例乙肝患者和30名健康者(对照组)外周血T淋巴细胞亚群(CD3 CD4 、CD3 CD8 )、NK细胞,免疫散射法检测血清免疫球蛋白(IgG、IgM、IgA)变化.结果表明各临床类型乙肝患者NK细胞降低,与对照组比较有显著性差异(P<0.01);慢性乙型肝炎组、慢性重型乙型肝炎组、肝硬化组外周血CD3 CD4 、CD3 CD8 T细胞均下降,其中慢性重型乙型肝炎组外周血CD3 CD8 与对照组比较有显著性差异(P<0.01),急性乙型肝炎组外周血T细胞亚群变化不明显(P>0.05);各临床类型乙型肝炎患者血清免疫球蛋白IgG、IgA随着病情的进展逐渐升高,与对照组比较有显著性差异(P<0.01).因此认为慢性乙肝患者存在细胞免疫和体液免疫功能紊乱,免疫功能的检测对乙肝的诊断、治疗及预后的判断有着一定临床意义.     

重型乙型肝炎前期患者外周血免疫活性细胞的特点

目的 分析重型乙型肝炎前期(以下简称重肝前期)患者外周血免疫活性细胞的特点.方法 选取重肝前期患者48例,慢性乙型肝炎患者35例,健康志愿者20例,采用流式细胞仪检测外周血淋巴细胞CD3+、CD3+/CD4+、CD3 +/CD8+、CD4 +/CD25 +/CD45+等亚群表达百分比,计算各淋巴细胞亚群绝对值,并进行统计学分析.结果 与慢性乙肝组及健康对照组相比,重肝前期组CD3+T细胞、CD8+T细胞、CD4+ CD25+调节性T细胞(Tregs)的绝对值均明显下降(P＜0.01或P＜0.05);重肝前期组CD4+T细胞绝对值与慢性乙肝组差异无统计学意义(P＞0.05),但CD4+T细胞百分率明显高于慢性乙肝组(P＜0.05).此外,CD4 +/CD8+比值各组间存在显著差异,重肝前期组显著高于慢性乙肝组和健康对照组(P＜0.01或P＜0.05).结论 重型乙型肝炎前期存在一定程度的细胞免疫功能紊乱,其特征为CD4+T细胞占优势,CD8+T细胞和CD4+ CD25+ Tregs绝对值下降.     

慢性肾衰血透患者TTV检测及其与细胞免疫功能的关系

目的　观察慢性肾衰 (CRF)血液透析 (HD)患者中 ,输血传播病毒 (TTV)的感染与患者细胞免疫功能的关系。方法　应用巢式逆转录 -聚合酶链反应 (RT PCR) ,双抗体夹心ELISA法及流式细胞仪 ,分别检测了 90例CRF血透患者和 12 8例对照组血清TTV DNA ,可溶性白细胞介素 2受体 (sIL 2R)和外周血T淋巴细胞亚群。并随机选择对其中1株TTV的部分基因序列进行测定 ,分析TTV与细胞免疫功能、输血次数、HD时间和肝功能的关系。结果　⑴ 90例CRF血透患者中 ,TTV DNA阳性率为 46 6 7% ,明显高于正常对照组 (P <0 0 0 1) ,与日本报道的TTV DNA序列(AB0 0 8394)相应片段的同源性为 98 5 %。⑵CRF血透患者血清中 ,sIL 2R水平明显高于正常对照组 (P <0 0 1) ;CD3 ,CD4 和CD4 /CD8 T细胞比例明显降低 (P <0 0 1)。⑶TTV DNA阳性率与sIL 2R、输血次数及HD时间呈显著的正相关 (r =0 486 ,P <0 0 1;r =0 .5 86 ,P <0 0 0 1;r =0 492 ,P <0 0 1) ,与CD ,CD4 和CD4 /CD8 T细胞比例呈负相关 (r = 0 476 ,P <0 0 1;r = 0 483,P <0 0 1;r= 0 496 ,P <0 0 1) ,而与年龄、性别及手术史均无显著的差别和相关性。结论　CRF血透患者有严重的TTV感染 ;其高发生率可能与细胞免疫功能明显低下及其与血液紧密接触有关     

自身CD3AK细胞治疗乙型肝炎的临床观察（附58例报告）

目的 :探讨CD3AK细胞治疗慢性乙型肝炎的临床治疗效果。方法 :将 1 0 8例慢性乙型肝炎患者随机分为两组。治疗组 5 8例患者接受CD3AK细胞治疗 (1疗程回输细胞总数为 1 1～ 8×1 0 1 0 ) ,对照组 5 0例。结果 :5 8例患者经CD3AK细胞治疗后 ,HBeAg转阴率 36 .3% ,ALT复常率达86 .1 % ,HBVDNA转阴率 6 2 .1 % ,与对照组相比较有显著差异 ;随访一年后HBeAg转阴率 6 7.9% ,HBeAg转换率为 2 0 .7% ,ALT复常率达 97.2 % ,HBVDNA转阴率 86 .2 % ,与对照组相比较仍有显著差异。治疗组CD3和CD8T淋巴细胞绝对值在CD3AK细胞治疗后均增加在 85 %以上 ,前后对比有显著性差异 (P <0 .0 1 )。结论 :自身CD3AK细胞过继免疫治疗乙型肝炎可有效提高患者HBeAg阴转率和ALT的复常率 ;能明显增强患者的细胞免疫功能     

Ⅱ型树突状细胞在慢性乙型肝炎患者中的功能检测及其临床意义

目的 研究慢性乙型肝炎患者外周血中Ⅱ型树突状细胞(pDC2)的数量及其功能的变化与疾病的发生发展的关系.方法 采用流式细胞分析技术对27例慢性乙型肝炎患者和15例健康人外周血中pDC2数量的变化以及其产生α干扰素的能力进行了检测,同时与T淋巴细胞亚群CD4+/CD8+的比值进行了比较分析.结果 肝炎患者外周血pDC2的百分数(0.096±0.086)较正常人(0.304±0.093)明显降低,两者差异有统计学意义(P＜0.001),特别是慢性肝炎患者pDC2产生IL-12及IFNα的能力明显低于正常人群(P＜0.001);而CD4+/CD8+的比值则明显高于正常人(P＜0.01).同时发现在HBV DNA(+)的患者中其pDC2的百分数和CD4+/CD8+的比值分别为0.087±0.054和1.95±0.562,均明显高于HBV DNA(-)患者(分别为0.043±0.031和1.44±0.476),差异有统计学意义(P＜0.01).结论 与正常人相比,慢性乙型肝炎患者外周血中pDC2的数量和其产生细胞因子的能力降低,后者可能与慢性乙型肝炎患者病程的进展以及血清中HBV DNA阳性有关.     

慢性乙型肝炎患者HBV特异性细胞毒性T细胞PD-1的表达研究

目的 检测慢性乙型病毒性肝炎患者外周血中HBV特异性CD8 细胞毒性T淋巴细胞表面细胞程序性死亡受体1(programmed cell death 1,PD-1)的表达情况.方法 采集慢性乙型肝炎患者外周血,直接离体情况下利用MHC-I-肽-五聚体技术标定HBV表位特异性细胞毒性T淋巴细胞以及荧光抗体标记细胞表面PD-1分子,经流式细胞仪检测分析.结果 在慢性乙型肝炎患者,HBV核心抗原18-27特异性CTL表达PD-1上调,达(79.0±12.5)%,显著高于总CD8 T细胞(27.7±14.8)%,以及CMV特异性CTL(20.6±5.9)%.结论 慢性乙肝患者HBV特异性CTL高表达PD-1分子,可能与慢性乙肝患者HBV特异性CTL功能低下密切相关.     

Flow cytometry CD4+/CD8+ ratio of liver-derived lymphocytes correlates with viral replication in chronic hepatitis B.

T lymphocytes have been assumed to play an essential role in tissue injury in patients with chronic hepatitis B. As hepatitis B virus (HBV) is considered as a major factor controlling liver inflammation, we assessed whether a particular T lymphocyte subset could be preferentially detected in the liver in accordance with viral replication. Liver-derived lymphocytes and peripheral blood lymphocytes were analysed by flow cytometry in 21 patients with histologically confirmed chronic hepatitis B without cirrhosis. Viral replication was quantified by hybridization of serum HBV DNA. Eleven patients exhibited an active viral replication with serum HBV DNA ranging from 10 to 388 pg/ml at the time of the liver biopsy, whereas 10 patients had no detectable serum HBV DNA. In patients exhibiting viral replication, CD4+/CD8+ ratios of liver-derived lymphocytes were significantly higher (P < 0.05) than those obtained in patients without viral replication. In contrast, the percentage of T cells expressing the gamma/delta receptor and that of CD2+/CD57+ cells were similar in both groups of patients. Furthermore, in patients exhibiting viral replication, CD4+CD8+ ratios of liver-derived lymphocytes correlated with serum HBV DNA levels (P < 0.001). No relationship between CD4+/CD8+ ratio of liver-derived and peripheral blood lymphocytes was observed. Our data indicate that, in patients with chronic hepatitis B, the CD4+/CD8+ ratio of liver-derived lymphocytes correlates with viral replication. This suggests that in situ helper/inducer CD4+ T lymphocytes may positively regulate the cytotoxic T cell activity in patients with HBV-related chronic hepatitis.     

小儿慢性丙型肝炎外周血T细胞亚群和TH1/TH2型细胞因子的研究

目的通过研究小儿慢性丙型肝炎外周血T细胞亚群及TH1/TH2型细胞因子的表达,进一步探讨小儿慢性丙型肝炎的免疫发病机制。方法(1)流式细胞仪(FACS)检测16例慢性丙型肝炎患儿及10例正常对照外周血T细胞亚群。(2)将慢性丙型肝炎患儿和正常对照外周血单个核细胞(PBMC)体外培养72h后,用ELISA法检测培养上清中TH1型细胞因子(IFN-γ、IL-2、IL-12和TNF-γ)和TH2型细胞因子(IL-4、IL-10)的浓度。结果(1)CD4 细胞无明显变化。CD8 细胞与正常对照比较明显升高(P<0.05)。CD3 细胞升高,CD4 /CD8 比值下降,但与正常对照比较无统计学意义(P>0.05)。(2)PBMC培养上清中IFN-γ、IL-10和TNF-α的水平明显升高(P<0.01),而没有检测到IL-2、IL-4、IL-12的基础分泌。结论慢性丙型肝炎患儿体内T淋巴细胞存在数量和功能的异常,CD8 细胞数升高,CD4 细胞功能异常,表现在以TH2型细胞因子的分泌为主。这可能与丙肝病毒(HCV)感染的慢性化有关。     

荧光定量聚合酶链反应检测慢性乙肝患者HBV DNA的意义

目的探讨荧光定量聚合酶链反应(FQ-PCR)检测慢性乙肝患者血清乙型肝炎病毒(HBV)脱氧核糖核苷酸(DNA)的临床意义。方法回顾性分析248例慢性乙肝患者的资料,均采用FQ-PCR技术检测血清HBV DNA,检测乙肝病毒标志物(HBV-M)并对比不同HBV-M患者血清HBV DNA水平;对比不同病情患者血清HBV DNA水平;对比不同HBV DNA表达患者外周血T淋巴细胞亚群水平及异常率,分析患者血清HBV DNA水平与外周血T淋巴细胞亚群水平的关系。结果不同HBV-M患者血清HBV DNA水平对比:HBsAg+HBeAg+HBcAb>HBsAg+HBeAg>HBsAg+HBsAb+HBcAb>HBsAg+HBeAb>HBsAb+HBeAb+HBcAb>HBcAb/HBsAb+HBeAb/HBeAb+HBcAb,除HBcAb、HBsAb+HBeAb、HBeAb+HBcAb血清HBV DNA水平差异无统计学意义(P>0.05),其余每2样本比较差异均有统计学意义(P<0.05);不同病情患者血清HBV DNA水平对比:重度病情患者>中度病情患者>轻度病情患者(P<0.05);不同HBV DNA表达患者CD3+、CD4+、CD4+/CD8+对比,HBV DNA阴性患者>低拷贝患者>高拷贝患者(P<0.05),CD3+、CD4+、CD4+/CD8+异常率对比,HBV DNA阴性患者<低拷贝患者<高拷贝患者(P<0.01);本组患者血清HBV DNA水平与外周血CD3+、CD4+、CD4+/CD8+均呈负相关(r=-0.789、-0.812、-0.706,P=0.012、0.007、0.001)。结论在慢性乙肝患者中FQ-PCR检测血清HBV DNA水平与HBV-M、病情和外周血T淋巴细胞亚群水平均有密切关系。     

Lymphocyte and macrophage phenotypes in chronic hepatitis C infection. Correlation with disease activity.

The pathogenesis of chronic hepatitis C and the mechanisms underlying progressive liver disease in patients with chronic hepatitis C infection are poorly understood. To demonstrate which inflammatory cells might be responsible for the necroinflammatory damage in chronic hepatitis C infection, we have correlated the phenotype of the intrahepatic lymphocytes and macrophages with histological activity in liver biopsy and explant specimens from 19 patients with chronic hepatitis C infection. In all stages of disease, more CD8+ than CD4+ lymphocytes were found. However, histologically active versus histologically mild hepatitis was associated with a trend toward greater parenchymal concentrations of CD4+ lymphocytes (0.71 +/- 0.27 per 10(4) microns 2 versus 0.35 +/- 0.15; not significant), significantly less parenchymal CD8+ lymphocytes (0.90 +/- 0.1 versus 1.70 +/- 0.3; t = 2.32, P = 0.03) and a greater parenchymal CD4/CD8 ratio (4.1 +/- 2.8 versus 0.91 +/- 0.3; t = 1.65, P = 0.07). No difference was found in the number of cells containing cytotoxic granules between the two groups. Greater numbers of CD4+ lymphocytes were found in liver biopsy specimens with little or no staining for hepatitis C virus antigen (1.47 +/- 0.88 versus 0.27 +/- 0.27; t = 2.28, P < 0.05). No significant differences were found in the macrophage subsets between the three stages of disease. Our data suggest that active histological disease in chronic hepatitis C infection may be associated with an increase in CD4+ lymphocytes and suggest that CD4+ T cells may play an important role in the hepatic injury in these patients.     

乙型肝炎病毒感染者外周血CD4+T细胞CD25、CD127不同亚群表达的检测及临床意义

目的 研究乙型肝炎病毒(HBV)感染者外周血CD4+T细胞表面CD25、CD127不同亚群的表达情况及临床意义。方法 用荧光抗体CD127-FITC、CD4-PECY5、CD25-PE标记T细胞。用流式细胞仪分别测定53例慢性乙型肝炎患者和53例HBV携带者CD4+T细胞表面CD25、CD127不同亚群的表达情况。对20例HBV-DNA阳性乙型肝炎病毒感染者干扰素治疗进行随访。结果与健康对照组[7.26％(6.15％,8.50％)]比较,慢性乙型肝炎患者[11.23％(9.10％,14.86％)]、HBV携带者[13.34％( 10.73％,18.90％)]CD25-CD 127-均显著升高,差异均有统计学意义(Q=4.559,P＜0.05;Q=6.230,尸＜0.05)。慢性乙型肝炎患者CD25hiCD127low/-[8.78％ (7.62％,10.44％)]显著高于健康对照[6.76％(5.73％,8.23％)]和HBV携带者[6.99％(5.77％,9.34％)],差异均有统计学意义(Q=3.497,P＜0.05;Q=3.103,P＜0.05)。HBV-DNA阳性组CD25-CD127-显著低于阴性组,两者差异有统计学意义[(12.92±5.20)％比(15.78±6.91)％,t=2.290,P=0.024],而CD25+/-CD127+显著高于阴性组,两者差异有统计学意义[(79.27±5.20)％比(76.02±7.04)％,t=2.194,P=0.030]。与治疗前比较,干扰素治疗12周CD25hiCD127low/-显著升高[(9.29±2.51)％比(11.08±2.38)％,t=2.820,P=0.011],而CD4+CD25-CD127-显著降低,两者差异有统计学意义[(13.86±5.72)％比( 10.86±3.60)％,t=2.469,P=0.024]。结论 HBV感染者外周血CD4+T细胞中CD25-CD127-亚群的表达与病毒的感染和清除有关;CD25hiCD 127low/-亚群的表达升高与发病有关。外源性干扰素可升高CD25hiCD127low/-的表达,降低CD25-CD127-的表达,从而抑制免疫反应。     

Abnormalities in CD4+ T lymphocyte subsets in patients with common variable immunodeficiency.

In order to investigate whether deficient immunoglobulin production in common variable immunodeficiency (CVI) patients was related to defective T cells functions, phenotype and proliferative responses to mitogen of peripheral blood mononuclear cells (PBMC) were investigated in 9 patients with CVI. The results were compared to those of 12 age- and sex-matched normal controls. The numbers of CD3+ and CD8+ T cells in the patients were not different from those in the control group, but the numbers of CD4 T cells were decreased (511 +/- 237 vs 844 +/- 247/mm3; P less than 0.01). The decrease in CD4 T cells was due to a dramatic deficiency in the CD4+ CD45RA+ subset, observed as an absolute value of blood lymphocytes (126 +/- 91 vs 384 +/- 142; P less than 0.001) and as a percentage (9.0 +/- 7.1 vs 18.8 +/- 5.0; P less than 0.01). In contrast, the CD4+ CD29+ T cell subset was not different in CVI from those in the control group. Moreover, there was a strong positive correlation between the number of percentages of CD4+ CD45RA+ blood T cells and the proliferative response of PBMC to PHA (respectively, P less than or equal to 0.02 and P = 0.05) and to Con A (P less than or equal to 0.02). The decrease of CD4+ CD45RA+ T cells could reflect an abnormality in the physiological status of T cells and could be of critical importance in the antibody deficiency.     

Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects

BACKGROUND: Increased production of IL-4 and IL-5 and decreased production of IFN-gamma by CD4+ T cells has been implicated in asthma pathogenesis. However, CD8+ T cells also produce type 1 and type 2 cytokines and the relative roles of CD4+ and CD8+ T cell cytokine production in asthma have not been previously studied. OBJECTIVE: To determine the production of the type 1 and type 2 cytokines by CD4+ and CD8+ T cell subsets in asthmatic and normal subjects. METHODS: Intracellular cytokine staining for IL-4, -5, -10, -13 and IFN-gamma was analysed in peripheral blood CD4+ and CD8+ T cells from 24 atopic asthmatic and 20 normal subjects. RESULTS: Both subsets of T cells produced all cytokines studied and there were no significant differences between CD4+ and CD8+ T cells in their capacity to produce either type 1 or type 2 cytokines. There were significantly increased frequencies of IFN-gamma-positive CD4+ (13.1 +/- 2.4%, vs. 7.3 +/- 1.4%) and CD8+ (20.0 +/- 2.9%, vs. 9.6 +/- 2.1%) T cells in asthmatic subjects compared with normal subjects (P < 0.05), but not in frequencies of CD4+ or CD8+ T cells staining positively for IL-4, -5, -10 or -13. CONCLUSION: The frequencies of peripheral blood CD8+ T cells producing type 1 and type 2 cytokines are comparable with the frequencies of CD4+ T cells. There was an increased frequency of IFN-gamma producing CD4+ and CD8+ T cells in asthmatic compared with normal subjects. Further studies investigating T cells derived from the airways and investigating various stages within the disease process are required to further elucidate the importance of type 2 and type 1 T cell cytokine production in the pathogenesis of human allergic disease.     

慢性乙肝病毒携带者外周血皮质醇水平与T细胞亚群状态的研究

目的 探讨慢性乙肝病毒(HBV)携带者血浆皮质醇水平与细胞免疫状态的变化.方法 选取符合诊断标准的慢性HBV携带者60例,正常对照10例,放射免疫法测定血浆皮质醇水平,流式细胞仪检测外周血T细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+比例.结果 与正常对照组相比,慢性HBV携带者血浆皮质醇水平相对升高(P＜0.05),CD4+细胞比例无明显变化(P＞0.05),CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).HBeAg阳性组与HBeAg阴性组比较,HBeAg阳性组血浆皮质醇升高较为明显(P＜0.05),CD4+细胞比例无明显变化(P＞0.05).CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).结论 慢性乙肝病毒携带者存在着血浆皮质醇水平的增高及细胞免疫功能的失衡,慢性HBV感染过程中存在内分泌-免疫系统紊乱.     

重型乙型病毒性肝炎肝组织内T淋巴细胞亚群的观察

本文用抗CD_3,CD_4,CD_8McAb和ABC法检测21例重型乙肝患者肝大片,亚大片坏死区炎性浸润细胞中的T细胞亚群,CD_3~+细胞>70％,其中主要为CD_8~+细胞,CD_4~+细胞减少,CD_4~+/CD_8~+比值显著下降,与急黄肝和慢活肝比较差异显著。相反,非HBV感染性肝病患者非T细胞和CD_4~+细胞增加,CD_4~+/CD_8~+比值>1。提示重肝时T细胞可能参与了肝损伤,CD_8细胞亚群可能是介导肝细胞坏死的重要因素之一。还观察到相当数量的淋巴细胞和肝细胞HLA—DR抗原阳性,淋巴细胞与膜型HBAg(+)肝细胞密切接触。