Effects of 30 cases of nervous olfactory disorder treated with acupuncture at three nasal points and scalp acupuncture

Objective:To observe the clinical therapeutic effects on nervous olfactory disorder treated with acupuncture at three nasal points and scalp acupuncture.Methods:Acupuncture was applied in 30 eligible patients with nervous olfactory disorder.The points included three nasal points,i.e.,Yíngxiāng(迎香 LI 20),Shàngyíngxiāng(上迎香 EX-HN 8)and Yìntáng(印堂EX-HN 3),ézhōngxiàn(额中线 middle line of forehead,MS1)and bilateral Nièhòuxiàn(颖后线 posterior temporal line,MS11).The even-needling technique was used to stimulate the three nasal points to ensure deqi. In scalp acupuncture,the needle was rotated continuously at 200 times per min,for 2 min at each scalp acupuncture line.The needles were retained for 40 min at above points and lines and were manipulated once every 20 min during needle retaining.The treatment was given once a day,6-day treatment was as one course and the next course of treatment started after the interval of 1 day.Totally,4 courses of treatment were required.Before and after treatment,TT olfactory was adopted to test the olfactory function and the score was compared in order to evaluate the therapeutic effect.Results:Of 30 cases,8 cases were cured,20 cases remarkably effective and 2 cases failed.The total effective rate was 93%.Conclusion:Acupuncture at three nasal points and scalp acupuncture are effective approach to nervous olfactory disorder.     

Effect of externally applied Jidesheng anti-venom on skin and soft-tissue necrosis after Chinese cobra bite： a retrospective study

OBJECTIVE： To evaluate the effects of Jidesheng anti-venom used externally for skin and soft-tissue necrosis from Chinese cobra bite. METHODS： A retrospective review was performed according to the clinical data recorded from Janu- ary 2002 to December 2012. A total of 126 patients （116 females and 10 males） with skin and soft-tis- sue necrosis due to Chinese cobra bite were divided into two groups. The control group was treated externally with 40% glyceride magnesium sulfate （n=52）, and the treatment group was given Jidesheng anti-venom externally （n=74）. The data collected included maximum local necrotic area of skin and soft tissues, de-tumescence time, healing time, and skin-grafting rate. RESULTS： There were no significant differences in gender, age, and disease condition between the control and treatment groups （P〉0.05）. No statistically significant difference was found in de-tumes- cence time between the two groups （P〉0.05）. The maximum local necrotic area of skin and soft tissues was （19.9 ± 7.3） cm2 in the treatment group, while it was （23.3 ±6.4） cm2 in the control group. The healing time of the treatment group was shorter than that of the control group [（32.1 ± 3.7） vs （34.4±4.5） days）] The skin-grafting rate in the treatment group was lower than that of the control group （10.81% vs 25.00%）. There were statistically significant differences in maximum local necrotic area of skin and soft tissues, healing time, and skin-grafting rate between the control and treatment groups （all P〈0.05）. CONCLUSION： External application of Jidesheng anti-venom may help to promote wound healing and reduce the skin-grafting rate in cases of skin and soft-tissue necrosis due to Chinese cobra bite.     

Baduanjin exercise improved premenstrual syndrome symptoms in Macau women

OBJECTIVE： To investigate the effect of Baduanjin,a traditional Chinese medical exercise, on improving premenstrual syndrome（PMS） symptoms in women.METHODS： Forty reproductive age women with PMS in Macau practiced standardized Baduanjin exercise for three menstrual cycles. A questionnaire,the daily record of severity of problems（DRSP）, was used to measure symptom severity. DRSP was filled out every days tarting from the last cycle before exercise（i.e., the first menstrual cycle） to the third cycle after exercise initiation（i.e., the fourth menstrual cycle）. The total scores and the scores of each item during the 5 premenstrual days and follicular phase（5-9 postmenstrual days）were calculated.RESULTS： After exercise, the total DRSP scores dur-ing the 5 premenstrual days and differences in the total DRSP scores between the 5 premenstrual days and the follicular phase were both significantly reduced. Of note, the physical symptom total scores in the 5 premenstrual days and the differences between its total score in the 5 premenstrual days and in the follicular phase were both significantly reduced. The differences between the total scores of depressed mood, anxious mood, loss of interest,and reduction in social activity during the 5 premenstrual days and the follicular phase were also reduced.CONCLUSION： Baduanjin exercise was able to improve the mental and especially the physical symptoms of PMS.     

电针对喹啉酸毁损大鼠海马的保护作用

Objective To probe into the protective mechanism of electro-acupuncture （EA） on anti-oxygen stress in dementia-model rats. Methods A total of 30 male SD rats of cleaning grade were randomized into three groups, named sham-operation （n = 10）, model （n = 10）, model＋ EA （n = 10）. The dementia-model rat was made with hippocampal GA1 lesions by quinolinic acid （QA）. On the second day of modeling, in model＋ EA group, EA （3 Hz, 2-4 mA, continuous waves） was applied to ＂Dàzhuī＂ （大椎 GV 14） and ＂Shènshū＂ （肾俞 BL 23） for 10 min. The capability of learning and memory was evaluated by step-down test to observe the changes of nitric oxide （NO）, nitric oxide synthase （NOS） activity, superoxide dismutase （SOD） activity and malondialdehyde （MDA） of the hippocampal tissues in rats separately by biochemical methods. Results EA improved learning and memory of dementia rats with hippocampal CA1 lesions induced by QA at different degrees. It significantly increased SOD activity, reduced MDA content and improved NO level and NOS activity. Conclusion EA improves significantly the learning and memory of the dementia rats due to hippocampal CA1 lesions induced by QA, which is probably relevant with the anti-oxygen stress of EA.     

Evaluation of hypoglycemic and hypolipidemic activity of methanol extract of Brassica oleracea

AIM： The hypoglycemic and hypolipidemic effects of the methanol extract of Brassica oleracea var. capitata（MEB） was evaluated in alloxan-induced diabetic rabbits. METHOD： The study was conducted on twenty-eight healthy white rabbits of either sex. All animals were equally divided into four groups. After confirmation of hyperglycemia, the animals of the treated and standard groups were administered MEB（500 mg·kg-1） and glibenclamide（10 mg·kg-1）, respectively for 15 and 30 days. The animals of the normal and diabetic controls received normal saline 1 mL/day equivalent to the volume of doses given to the test and standard animals. Biochemical tests were performed at the end of dosing, i.e. the 16 th and 31 st days. RESULTS： The MEB revealed a decrease of 106.6 mg·dL-1 in fasting blood glucose as compared to diabetic control, which was almost comparable to glibenclamide; both of these changes were highly significant. The decrease in total cholesterol and low density lipoprotein was 94.3 and 96.5 mg·dL-1, respectively, whereas the high-density lipoprotein was increased by 26.7 mg·dL-1, as compared to diabetic control. All of the changes in lipid profile were statistically significant. CONCLUSION： These results suggest the potential of MEB as a hypoglycemic and hypolipidemic agent.     

An HPLC-MS/MS method for the quantitative determination of platy-codin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract简

AIMS： To develop an HPLC-MS/MS method for the quantification of platycodin D （PD） in rat plasma, and to acquire the main pharmacokinetic parameters of PD after oral administration of pure PD or of Platycodi Radix extract （PRE） containing PD. METHOD： Plasma samples were pretreated with solid-phase extraction using an Oasis HLB SPE cartridge. Madecassoside was used as the internal standard （IS）. Chromatographic separation was achieved on an ODS column （100 mm x 2.1 mm i.d., 3.5 μm） with a mobile phase consisting of acetonitrile/water （30 ： 70, V/F） containing 0,1 mmol·L-1 ammonium acetate at a flow rate of 0.25 mL.min-1. The detection was performed on a triple quadruple tandem mass spectrometer using an electros- pray ionization （ESI） source with a chromatographic rtm time of 3.0 rain. The detection was operated by multiple reaction monitoring （MRM） of the transitions of m/z 1 223.6→469.2 for PD and of m/z 973.6→469,2 for madecassoside （IS）, respec- tively. RESULTS： The calibration curve was linear from 5 to 2 000 ng.mL-1 （re 〉0.99） with a lower limit of quantification （LLOQ） of 5 ng.mL^-1. The intra- and inter-day precision （relative standard deviation, RSD） values were below 15% and the accuracy （relative error, RE） was from -15% to ＋15% at three quality control （QC） levels. Plasma concentrations of PD were deter- mined for 24 h after i.v. administration of PD, and oral administration of PD and PRE, respectively. The absolute oral bioavailability of PD in rats was found to be （0.48 ± 0.19）% when administered PD, and to be （1.81 ± 0.89）% when adminis- tered PRE. CONCLUSION： The developed HPLC-MS/MS method was successfully applied to assess the pharmacokinetic parameters and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract.     

11 CASES OF MOTOR INJURY OF ANTERIOR TALOFIBULAR LIGAMENT TREATED BY ACUPUNCTURE OF QIUXU(GB 40)-THROUGH-ZHAOHAI(KI 6)POINT

Injury of ankle is the most common in athletic injury.Because of anatomic particu- larity of ankle joint,i.e.lateral malleolus is long and thin and is located at the posterior and lower than medial malleolus.The medial malleolus is broad and flat and is located at the anterior.Lateral ligament is thinner and weaker than medial ligament and the muscle groups supporting strephexopodia is far inferi- or to the muscle groups supporting strephenopodia in strength,injury of ankle joint happens more at inversion sprain and in- jury of anterior talofibular ligament is induced often.Thus,rapid and effective treatment of anterior talofibular ligament injury can elevate better athletics of sportsman.Whenever the disease happened in students to exercise,the authors often used acupuncture of Qiuxu（GB 40）-through-Zhaohai（KI 6）point with strong stimulative manipulation,which could relieve more remakedly pain of the ankle joint caused by anterior talofibular ligament injury as re- ported in the     

Protective effect of turmeric extract on ethotrexate-induced intestinal damage and oxidative stress

AIM： The most important side effect of methotrexate （MTX） is mucositis. The purpose of this study was to evaluate the effect of turmeric extract on intestinal damage and oxidative stress in rats receiving methotrexate. METHODS： Experiments were per- formed on male Wistar albino rats divided into six groups. First group received normal saline orally, the second group received turmeric extract （100 mg.kg-1） orally for 30 days, the third group received turmeric extract （200 mg.kg-I） orally for 30 days, the fourth group re- ceived a single dose of methotrexate （20 mg.kg-~） i.p. at day 30, the fifth group received turmeric extract （100 mg.kg-1） orally for 30 days and a single dose of methotrexate （20 mg.kg-1） i.p. at day 30, and the sixth group received turmeric extract （200 mg.kg-1） orally for 30 days and single dose of methotrexate （20 mg.kg-1） i.p. at day 30. Four days after methotrexate injection, animals were anesthetized, blood samples were taken to determine total antioxidant status （TAS） and jejunum samples were taken for glutathione peroxidase （GPx）, super- oxidase dismutase （SOD）, catalase （CAT）, aldehyde malondialdehyde （MDA）, and histopathological assessment. RESULTS： Microscopic evaluation from intestinal tissues of the MTX treated group, showed severe villus shortening and blunting, inflammatory cell infiltration and hemorrhage in lamina propria, along with epithlial cell necrosis. Levels of SOD, GSH-Px and CAT decreased in the MTX received group, but increased significantly （P 〈 0.05） in the turmeric ＋ MTX groups. MTX increased lipid peroxidation, however, turmeric decreased peroxidation significantly （P 〈 0.05）. CONCLUSION： These results suggest that turmeric extract may protect the small intes- tine of rats from methotrexate-induced damage. Turmeric effects could result from its antioxidant properties.     

Busuishengxue ranules mediate their effects upon non-severe aplastic anemia via mitogen-activated protein kinase/extracellular signal-regulated kinase pathway

OBJECTIVE：To observe the clinical efficacy of Busu- ishengxue granules on non-severe aplastic anemia（NSAA） and investigate its effect on the mitogen-ac- tivated protein kinase/extracellular signal-regulat- ed kinase（MAPK/ERK） pathway. METHODS： Sixty NSAA patients were divided equally into two groups. Subjects in the experimen- tal group were treated with Busuishengxue gran- ules, and the control group with Zaizaoshengxue tablets.The treatment course was 6 months and cu-rative efficacy was compared between the two groups as well as with 10 healthy individuals. Flow cytometry（FCM） was used to detect the intracellu- lar concentration of Ca2 ＋（[Ca2 ＋]i）. Western blotting was employed to detect the expression of enzymes in the MAPK/ERK pathway. RESULTS： The efficacy of Busuishengxue granules was significantly better than that of Zaizaoshengx- ue tablets（P0.05）. Before treatment, expression of JNK, phospho-ERK 1/2 and p-JNK was higher, and [Ca2 ＋]i higher, than that of the control group（P 0.05）. After treatment with Busuishengxue gran- ules, expression of all enzymes related to signal transduction pathways in the blood cells of NSSA patients were altered to different degrees. CONCLUSION： Busuishengxue granules had a bet- ter effect with regard to improving symptom scores, increasing the number of blood leukocytes, and increasing hemoglobin levels than Zaizaosh- engxue tablets, and they differed slightly in terms of increasing the number of platelets.     

Hepatoprotective and in vivo antioxidant activities of the hydroethanolic leaf extract of Mucuna pruriens (Fabaceae) in antitubercular drugs and alcohol models

AIM： Hepatotoxicity is a significantly increasing health problem worldwide, and the extent of the problem has stimulated interest in the search for hepatotherapeutic agents from plants. This study investigated the hepatoprotective and in vivo antioxidant activities of the hydroethanolic extract of Mucuna pruriens leaves in antitubercular and alcohol-induced hepatotoxicity assays in rats. METHOD： In each of the models used, seven groups were allotted. The different groups received normal saline （10 mL·kg^-1, p.o.）; hepatotoxicant （isoniazid-rifampicin, INH-RIF, 100 mg·kg^-1, i.p. or 20% ethanol 5 g·kg^-1, p.o.） and normal saline （10 mL·kg^-1, p.o.）; hepatotoxicant and extract at doses of 100, 200, and 400 mg·kg^-1 p.o.; hepatotoxicant and silymarin 50 mg·kg^-1 p.o.; and extract at 400 mg·kg^-1 p.o.. On the 21st day of treatment, blood was collected for assessment of serum biochemical parameters and harvested liver samples were assessed for antioxidants. RESULTS： The hepatotoxicants significantly （P 〈 0.05-0.001） increased the levels of alanine transaminase （ALT）, aspartate transaminase （AST）, alkaline phosphatase （ALP）, bilirubin, and malondialdehyde （MDA）; and reduced the levels of catalase （CAT）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, and reduced glutathione GSH compared to control. M. pruriens significantly reversed （P 〈 0.05-0.001） the elevation in the level of ALT, AST, ALP, and bilirubin caused by the hepatotoxicants. The extract （200 and 400 mg·kg^-1） significantly reversed （P 〈 0.05） the diminution in the level of in vivo antioxidants and increased the level of MDA produced by INH-RIF. M. pruriens （100-400 mg·kg^-1） elicited significant reduction （P 〈 0.001） in the level of MDA compared to the alcohol group. Silymarin also reversed the deleterious effects of the hepatotoxicants. CONCLUSION： The hydroethanolic extract of Mucuna pruriens leaves possesses hepatoprotective activity with enhancement of in vivo antioxidants as a possible mechanism of action.     

Maslinic acid modulates glycogen metabolism by enhancing the insulin signaling pathway and inhibiting glycogen phosphorylase

AIM： To investigate the molecular signaling mechanism by which the plant-derived, pentacyclic triterpene maslinic acid （MA） exerts anti-diabetic effects. METHOD： HepG2 cells were stimulated with various concentrations of MA. The effects of MA on glycogen phosphorylase a （GPa） activity and the cellular glycogen content were measured. Western blot analyses were performed with anti-insulin receptor β （IRβ）, protein kinase B （also known as Akt）, and glycogen synthase kinase-3β （GSK3β） antibodies. Activation status of the insulin pathway was investigated using phospho-IRβ, as well as phospho-Akt, and phospho-GSK3β antibodies. The specific PI3-kinase inhibitor wortmannin was added to the cells to analyze the Akt expression. Enzyme-linked immunosorbent assay （ELISA） was used to measure the effect of MA on IRβ auto-phosphorylation. Furthermore, the effect of MA on glycogen metabolism was investigated in C57BL/6J mice fed with a high-fat diet （HFD）. RESULTS： The results showed that MA exerts anti-diabetic effects by increasing glycogen content and inhibiting glycogen phosphorylase activity in HepG2 cells. Furthermore, MA was shown to induce the phosphorylation level of IRβ-subunit, Akt, and GSK3β. The MA-induced activation of Akt appeared to be specific, since it could be blocked by wortmannin. Finally, MA treatment of mice fed with a high-fat diet reduced the model-associated adiposity and insulin resistance, and increased the accumulated hepatic glycogen content. CONCLUSION： The results suggested that maslinic acid modulates glycogen metabolism by enhancing the insulin signaling pathway and inhibiting glycogen phosphorylase.     

The Needle-Rolling Therapy for Treatment of Non-organic Chronic Insomnia in 90 Cases

Objective： To evaluate the therapeutic effects of needle-rolling therapy for chronic insomnia. Methods： In the present multi-central randomly controlled clinical study, 180 cases of chronic insomnia were randomly divided into the following two groups, a treatment group （90 cases） treated by the needle-rolling therapy and a control group （90 cases） treated with clonopin. The treatment course for both the two groups was 4 weeks. The therapeutic effects were evaluated based on improvement of the TCM symptoms and the Pittsburgs＇s sleep-quality index （PSQI）. Results： After treatment, there were significant differences between the two groups in the effective rate （P〈0.05）, and in the total score of PSQI and in the scores of the 4 sub-items, i.e. sleep-quality, sleep-efficiency, hypnotic and daytime function （P〈0.05）. Although there was no significant difference between the two groups in the effective rate after a 3-month follow-up period, significant differences still existed in the 3 sub-items of sleep-efficiency, hypnotic, and daytime function of the PSQI （P〈0.05）. Conclusion： As compared with hypnotics of the second generation, the needle-rolling therapy may show better therapeutic effects for chronic insomnia patients.     

Therapeutic efficacy observation on combining acupuncture and chinese herbal fumigation for cervical radiculopathy

Objective： To observe the clinical effect of needling eight neck points and Chinese herbal fumigation for cervical radiculopathy. Methods： A total of 85 cases who met the inclusion criteria of cervical radiculopathy were allocated into a Jiaji （EX-B 2） points group, an eight neck points group and a comprehensive therapy group according to single-blind randomized controlled trial design. The 27 cases in the Jiaji （EX-B 2） points group were treated with needling cervical Jiaji （EX-B 2） points. The 28 cases in the eight neck points group were treated with needling the eight neck points. The 30 cases in the comprehensive therapy group were treated with needling the eight neck points coupled with Chinese herbal fumigation over the affected area. Before and after treatment, the symptoms of traditional Chinese medicine （TCM） were graded and the short-form McGill pain questionnaire （SF-MQP） was employed to test pain rating index （PRI）, visual analogue scale （VAS） and present pain intensity （PPI）. In addition, therapeutic efficacies were compared among three groups. Results： After treatment, all SF-MPO, scores in three groups were significantly reduced, and there were inter-group statistical significant in comparison of scores differences between before and after treatment （P〈O.01）; there were inter-group statistical differences in the total effective rate （P〈O.05）. Conclusion： Needling the cervical Jiaji （EX-B 2） points and eight neck points can both alleviate pain in cervical radiculopathy patient. However, combining needling the eight neck points and Chinese herbal fumigation can obtain the better effect.     

Effect of moxibustion on the motility,absorption and activities of ATPase in small intestine of spleen-deficiency rats

Objective： To observe the effect of moxibustion on intestinal propulsion rate, content of serum D-xylose, content of adenosine triphosphate （ATP） and activities of adenosine triphosphatases （ATPase） in jejunum tissues of spleen-deficiency rats, and to explore the effect of moxibustion on the production of ATP and activities of membrane proteins, for analyzing the mechanism of moxibustion in reinforcing the spleen-stomach. Methods： Forty healthy Sprague Dawley rats were randomly divided into four groups, namely group A （blank control group）, group B （model control group）, group C （moxibustion group） and group D （herbs group）. The animal model of spleen-deficiency was established by intragastric administration with Da Huang （Radix et Rhizoma Rhei） infusion. The rats in group C received moxibustion at Zusanli （ST 36）, Zhongwan （CV 124 Guanyuan （CV 4）, Pishu （BL 20） and Weishu （BL 21）. The rats in group D received intragastric administration with 5i Jun Zi decoction. The intestinal propulsion was measured by toner method, and the content of D-xylose in serum was detected by phloroglucinol method. Colorimetry method was used to detect the content of ATP and activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in jejunum tissues. Results： Compared with group A, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATP in jejunum tissues decreased significantly in group B （P〈0.05 or P〈O.O1）. Compared with group B, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in jejunum tissues increased significantly in group C and D （P〈0.05 or P〈0.01）, while there were no significant differences between group C and group D. Conclusion： Moxibustion can increase the intestinal propulsion rate, content of D-xylose in serum and ATP in jejunum tissues, as well as the activities of Na~-K＋-ATPase and Ca^2＋-Mg^2＋-ATPase of spleen-deficiency rats, which suggests that moxibustion can enhance the motility and absorption functions of small intestine by promoting the production of ATP in intestinal epithelial cells, increasing the content of membrane proteins, and improving transmembrane transport.     

Effect of combining acupuncture and functional training on post-stroke functional impairment of hand

Objective： To observe the clinical effect of combining acupuncture and functional training on post-stroke functional impairment of hand. Methods： A total of 165 cases who met the inclusion criteria were randomized into a treatment group （85 cases） and a control group （80 cases）. Cases in the treatment group were treated with routine acupuncture points plus Shixuan （EX-UE 11） and Xiaohai （SI 8）, whereas cases in the control group were treated with routine acupuncture points alone. After acupuncture, cases in both groups conducted functional training in upper limbs. The finger grip strength and hand function were assessed before and after 3-course treatment. Results： Cases in the treatment group obtained better finger grip strength and hand function than those in the control group （both P〈0.05）. Conclusion： In addition to functional training, adding Shixuan （EX-UE 11） and Xiaohai （SI 8） to traditional acupuncture formulae works better for post-stroke functional impairment of hand.     

Regulatory Action of Charred Gossamer Urocteae on the Functions of Mouse Oral Fibroblasts

Objective： To explore the influence of charred Gossamer urocteae （CGU） on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing. Methods： CGU was extracted with different solvents and ethanol extract （EE）, ethyl acetate fraction （EF）, n-butanol fraction （BF） and aqueous fraction （AF） were obtained. The effects of different fractions on the proliferation, matrix metaUoproteinase-2,9 （MMP-2,9） activities, synthesis of collagen and tissue inhibitor of metalloproteinase 1 （TIMP-1） in the mouse oral fibroblasts were determined by MTT, gelatin zymography, chloramine-T method, and enzyme-linked immunosorbent assay （ELISA） respectively. Results： EE, EF and BF at high concentrations could significantly inhibit proliferation of fibroblasts （P〈0.05 or P〈0.01）, and at low concentrations EF and BF could promote proliferation of fibroblasts, and BF and AF could significantly inhibit collagen synthesis （P〈0.05 or P〈0.01）. EE, EF and AF at high concentrations could significantly increase the MMP-9 activity, and BF and AF could significantly inhibit synthesis of TIMP-1. Conclusion： CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen, and in healing of wound, CGU at high concentrations possibly has the functions of anti-fibrosis and anti-scar, and the mechanism to promote degradation of collagen is possibly related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.     

Observation on clinical effects of acupotomy plus cupping for knee osteoarthritis

Objective： To observe the clinical effect of acupotomy plus cupping for knee osteoarthritis（KOA）. Methods： Sixty cases with KOA were randomly divided into a treatment group and a control group, 30 cases in each group. The patients in the treatment group were given acupotomy and cupping, while the patients in the control group were given injection of Sodium Hyaluronate. The index of severity for osteoarthritis（ISOA）, the change of the effusion of knee joint and clinical effects were observed after treatment. Results： The total effective rate was respectively 96.7% in the treatment group and 66.7% in the control group. The difference in the clinical effects between the two groups was statistically significant（P〈0.05）. After treatment, ISOA scores and scores of knee effusion in the two groups were remarkably reduced than those before treatment（P〈0.05）. The changes of the scores of the two indexes were statistically significant（both P〈0.05）. Conclusion： Acupotomy plus cupping is better than injection of Sodium Hyaluronate in treatment of KOA.     

Treatment with Hqi Bushen Koufuye Combined with Chemotherapy for Preventing Postoperative Metastasis of Stomach Cancer ——A Clinical Observation of 28 Cases

To study the effect of Yiqi Bushen Koufuye （益气补肾口服液 Oral Liquid for Invigorating Qi and Tonifying the Kidney） combined with chemotherapy on postoperative metastasis of stomach cancer. Methods： The 47 cases of postoperative stomach cancer with the syndrome of deficiency of both the spleen and kidney were divided randomly into the treatment group （28 cases）, and the control group （19 cases）. The control group was treated simply by chemotherapy; while the treatment group, was treated with Yiqi Bushen Koufuye in addition to chemotherapy. The effect was observed 12 months later on local relapse and distal metastasis, the life quality, peripheral hemogram, and immunologic function. Results： The rates of postoperative relapse and metastasis of the treatment group were obviously lower than those of the control group （P〈0.05）. The Karnofasky scores, peripheral hemogram and immunologic function of the treatment group were obviously improved in comparison with the control group （P〈0.01 or P〈0.05）. Conclusion： Yiqi Bushen Koufuye combined with chemotherapy is effective in preventing postoperative metastasis of stomach cancer, increasing sensitivity and decreasing toxins, and improving the life quality and immunologic function of the patient.