Choroidal Neovascularization and Haller Vessel Morphology Associated with Vision and Treatment Number after 1 Year in Age-related Macular Degeneration

PurposeThe present study aimed to quantify various factors of vessel morphology, including vessel diameter, length, and complexity (fractal dimension and lacunarity) of both choroidal neovascularization (CNV) and en face Haller vessels using optical coherence tomography angiography (OCTA) and en face structural optical coherence tomography in typical neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV) and to identify factors associated with visual acuity (VA) loss and number of injections within a year after the day of OCTA.MethodsWe retrospectively analyzed 43 eyes of nAMD patients and 33 eyes of PCV patients whose OCTA was performed at least 12 months after an initial anti-vascular endothelial growth factor treatment. Quantitative parameters, including vessel area, vessel diameter, vessel length, fractal dimension, and lacunarity were analyzed from en face images of CNV and Haller vessels. Clinical information, including logarithm of the minimum angle of resolution visual acuity and injection number of anti-vascular endothelial growth factor were acquired after 12 months from OCTA date. Using logistic regression analyses, parameters associated with logarithm of the minimum angle of resolution VA loss of 0.2 or more (VA loss group) and a number of injections of four or more (unstable group) after 12 months were analyzed.ResultsIn typical nAMD, the VA loss group was associated with a smaller number of intersections of Haller vessels. The unstable group was associated with an increased lacunarity of CNV in typical nAMD. In PCV, both VA loss and unstable groups were associated with a higher maximal diameter of Haller vessels.ConclusionsVA loss and injection number of nAMD and PCV 12 months after OCTA imaging were associated with different morphological parameters of CNV and Haller vessels. Therefore, quantitative analyses of both CNV and Haller vessels from OCTA and en face optical coherence tomography might provide prognostic information about visual outcome and injection frequency within 12 months after OCTA imaging.     

Choroidal Vascular Abnormalities by Ultra-widefield Indocyanine Green Angiography in Polypoidal Choroidal Vasculopathy

PurposeTo evaluate vortex vein engorgement and choroidal vascular hyperpermeability in patients with polypoidal choroidal vasculopathy (PCV) using ultra-widefield indocyanine green angiography (ICGA).MethodsThis retrospective case control study included 51 patients with unilateral PCV, 7 patients with bilateral PCV, and 43 age-matched controls. The number of quadrants of vortex vein engorgement was evaluated in the middle phase of ICGA, which was classified as extended engorgement if the dilated choroidal vessels expanded to the macula. The area of choroidal vascular hyperpermeability was quantified stereographically from the late-phase ICGA and correlated with clinical and optical coherence tomography findings.ResultsAffected eyes had a larger choroidal hyperpermeability area and a thicker subfoveal choroid than eyes in the control group or fellow eyes (P < 0.001, P < 0.001). More quadrants with extended vortex vein engorgement were observed in affected eyes than in fellow eyes (P < 0.001). Significant differences were observed in the area of choroidal hyperpermeability, Haller layer thickness and greatest linear dimension according to the extended vortex vein engorgement in eyes with PCV (P < 0.001, P = 0.001, and P = 0.001, respectively). The area of choroidal hyperpermeability was significantly correlated with subfoveal choroidal thickness (P < 0.001, Pearson''s correlation coefficient = 0.471).ConclusionsUltra-widefield ICGA results revealed that patients with PCV had vortex vein engorgement and an increased choroidal hyperpermeability area. The results from this study provide substantial information to clarify the pathogenesis and predict the prognosis in the patients with PCV.     

康柏西普对实验性脉络膜新生血管的作用机制研究

目的　探究康柏西普对实验性脉络膜新生血管（choroidal neovascularization，CNV）的作用机制。方法　选取40只7周龄的雄性BN大鼠，采用多波长氪激光对豚鼠左眼行视网膜光凝，制作CNV大鼠模型，并随机分为4组，空白对照组、模型组、低剂量康柏西普组和高剂量康柏西普组；于造模后7 d、14 d注射康柏西普，并在造模后21 d进行右眼眼底彩照、眼底荧光素血管造影（fundus fluorescein angiography，FFA）检查，检测CNV 发生率及渗漏面积，使用FITC灌注脉络膜铺片测量CNV面积，使用HE染色观察大鼠视网膜结构，使用Western blot检测大鼠视网膜中血管内皮生长因子（vascular endothelial growth factor，VEGF）、缺氧诱导因子-1α（hypoxia-inducible factor-1α，HIF-1α）、丝氨酸/苏氨酸激酶 （serine/threonine kinase，AKT）表达情况。结果　除吲哚菁绿血管造影荧光点数、CNV发生率外，相比空白对照组，模型组大鼠CNV渗透面积，荧光素渗漏面积，脉络膜结构损伤程度，视网膜中 HIF-1α、AKT和VEGF蛋白的表达均显著升高，差异均有统计学意义（均为P<0.05）；相比模型组，康柏西普组大鼠上述各指标显著降低，高剂量康柏西普组上述各指标显著低于低剂量康柏西普组，差异均有统计学意义（均为P<0.05）。结论　康柏西普可以抑制脉络膜视网膜中 HIF-1α、AKT和VEGF蛋白的表达，从而抑制由氪激光光凝眼底而产生的实验性CNV生长。     

AAV介导的EPO基因下调对CNV的抑制作用

研究腺相关病毒（AAV）介导的促红细胞生成素（EPO）shRNA靶向视网膜色素上皮（RPE）的EPO表达水平下调对脉络膜新生血管（CNV）进展的影响，以期发现潜在的湿性年龄相关性黄斑变性（nAMD）的治疗靶点。方法：实验研究。在HEK293T细胞内对包含EPOshRNA片段的AAV质粒进行验证（采用Student's t检验）。选用2个月龄C57/B6J小鼠10只，右眼作为实验组，视网膜下注射AAV1-sCBA-GFP-EPO-shRNA；左眼作为对照组，视网膜下注射AAV1-sCBA-GFP。注射后3周，进行激光诱导的CNV造模，造模后拍摄眼底照片确认病毒转染及造模情况，造模后15 d RPE铺片以 测算CNV面积大小，并用Student's t检验方法进行组间比较。结果：EPO shRNA在HEK293T细胞内对EPO表达的下调效率为69.6%，且与对照组相比差异具有统计学意义（t=6.080，P=0.022）。眼底照片显示，注射了AAV的小鼠造模成功；视网膜有点状的绿色荧光蛋白表达，证实病毒转染成功。造模后15 d，RPE铺片显示实验组的平均CNV面积与对照组相比小44.7%（t=4.279，P=0.001）。结论：AAV介导的EPO shRNA对CNV的发生和发展起到显著的抑制作用，在未来可能作为nAMD的新治疗方法。     

Development of Secondary Choroidal Neovascularization in Focal Choroidal Excavation of Punctate Inner Choroidopathy

ABSTRACT

Purpose: To elaborate a case of focal choroidal excavation (FCE) in punctate inner choroidopathy (PIC) complicated by secondary choroidal neovascularization (CNV) based on multimodal imaging findings.

Methods: In this retrospective case report, multimodal imaging including near-infrared reflectance, blue peak autofluorescence, spectral domain-optical coherence tomography (OCT), fluorescein and indocyanine green angiography (Heidelberg Engineering GmbH, Germany), and swept source-OCT angiography (SS-OCTA; Topcon Corporation, Japan) was performed.

Patients: A 27-year-old moderate myopic woman presented with inactive CNV of unknown origin in her left eye, which had been previously treated with intravitreal anti-vascular endothelial growth factor.

Results: Multimodal imaging revealed PIC as the causative disease and systemic corticosteroids were administered. Similar complaints 13 months later showed new CNV formation at the already documented FCE. No sign of PIC could be detected at follow-up.

Conclusion: This well-documented case highlighted FCE as the preferential location for CNV development in PIC with multimodal imaging emphasizing a chorioretinal entity.

Summary: This case report demonstrated the clinical course of focal choroidal excavation in a patient initially diagnosed with punctate inner choroidopathy complicated by choroidal neovascularization and its treatment response, well documented by multimodal imaging including optical coherence tomography angiography.     

OCTA与ICGA检查在CNV和PCV中的影像对比分析

目的：对比观察脉络膜新生血管(CNV)与息肉状脉络膜血管病变(PCV)在吲哚菁绿血管造影(ICGA)与光学相干断层扫描血管成像(OCTA)中的特征性表现,探讨二者的区别和优缺点。

方法：回顾分析2018-09/2020-04在河北省眼科医院确诊的CNV患者26例34眼和PCV患者19例19眼的影像学资料,CNV患者中湿性年龄相关性黄斑变性(w-ARMD)20例28眼,慢性中心性浆液性脉络膜视网膜病变(CCSC)继发CNV 6例6眼。所有患者均行OCTA、荧光素眼底血管造影(FFA)+ICGA检查,分析其特征性改变。

结果：w-ARMD患者28眼OCTA检查示,除2眼因出血较多未见明显异常外,余26眼均可显示CNV形态,呈现效果形态更加锐利、立体,且能够分辨出CNV所在的解剖层面,其中11眼OCTA检查不仅能够很好地显示出CNV的形态、大小、范围,而且对于CNV中的滋养血管、新生血管及吻合支也都能较好地分辨。CCSC继发CNV患者6眼OCTA发现了FFA+ICGA未发现的CNV形态。PCV患者19眼OCTA检查异常脉络膜分支血管网(BVN)显示优于ICGA,但OCTA图像上末端囊袋样扩张(polyps)的显示及数量不如ICGA明显,部分不能显示。

结论：OCTA检测CNV阳性率较ICGA高,检测CNV和PCV病变范围较ICGA更清晰、立体,但在PCV中不能全部显示末端囊袋样扩张,且不能观察CNV或PCV渗漏情况及病变动态发展过程,较 FFA+ICGA尤为不足。OCTA作为无创检查可以随访观察CNV或PCV治疗前后病变发展情况。     

特发性息肉样脉络膜血管病变一例报告

目的：报道1例特发性息肉样脉络膜血管病变的眼底荧光血管造影与吲哚青绿血管造影的临床特点。方法：常规眼科检查、眼底照相、荧光血管造影、吲哚青绿血管造影分析以及文献复习。结果：特发性息肉样脉络膜血管病变患者缺乏典型的玻璃疣,但可在视乳头与黄斑间出现大量泥沙样黄色渗出,眼底荧光血管造影中没有老年性黄斑变性样的新生血管,吲哚青绿血管造影中可见典型的息肉样脉络膜血管扩张,可单眼发病。结论：特发性息肉样脉络膜血管病变在临床表现以及眼底血管造影方面均有特征性改变。区分特发性息肉样脉络膜血管病变与老年性黄斑变性在疾病的视力预后、转归以及治疗处理上都有实际的临床意义。     

当前监测新生血管性年龄相关性黄斑变性患者对侧眼的观念和方法——专家共识解读

新生血管性年龄相关性黄斑变性（nAMD）常双眼先后发病，一眼发病后如何保持对侧眼有用的视功能与患者的生活质量密切相关。因此，监测nAMD患者对侧眼的发病情况就尤为重要。近年，远景学院（Vision Academy）的眼底病专家发起了“当前监测nAMD患者对侧眼的观念和方法”专家共识，该共识提出早期发现nAMD患者对侧眼脉络膜新生血管的重要性，并对目前的监测方法进行了分析，推荐每3～4个月进行一次对侧眼的检查，并鼓励利用家用技术和设备进行监测，以期早期发现、及时治疗。     

Indocyanine green angiography in the juvenile haemorrhagic choroidopathy

Juvenile haemorrhagic choroidopathy (JHC) is an idiopathic syndrome marked by macular choroidal neovascularization (CNV) in patients under the age of 50. We used fluorescein angiography (FA) and indocyanine green angiography (ICGA) to examine 17 patients with macular CNV and JHC. CNV was always unilateral. On ICGA examination the CNV were weakly fluorescent in 59% of cases, hyperfluorescent in the remaining 41%.ICGA showed up the following alterations: a) areas with diffuse choroidal hyperfluorescence at the posterior pole or in the peripapillary region in 11 affected eyes (65%), in the fellow eye too in 5 patients; b) areas of choroidal hypofluorescence at the posterior pole but also outside the vascular arcades in 2 affected eyes (12%).In conclusion, ICGA does not appear indispensable for detecting CNV in JHC but this method does show up diffuse choroidal alterations not detectable with FA. The pathogenetic implications of the ICGA findings are discussed.     

Surgical Excision of Type 1 + 2 Choroidal Neovascularization in Age-Related Macular Degeneration

Purpose To evaluate the visual acuity outcome and the various factors influencing visual outcome in patients undergoing surgical removal of type 1 + 2 choroidal neovascularization (CNV) caused by age-related macular degeneration (AMD).Methods We studied the records of 54 patients (54 eyes) followed for at least 1 year after surgical excision of CNV associated with AMD.Results The final visual acuity was 0.4 or better in 4%, 0.1 to 0.3 in 46%, and worse than 0.1 in 50% of the patients. Final visual acuity was improved in 39%, stable in 37%, and worse in 24%. Stepwise regression showed that the distance between the center of the foveal avascular zone and the CNV margin had the greatest effect on final visual acuity.Conclusions Surgical excision of type1 + 2 CNV due to AMD may be indicated for preserving preoperative visual acuity. The distance between the center of the foveal avascular zone and the CNV margin is the most important factor affecting final visual acuity. Jpn J Ophthalmol 2005;49:324–326© Japanese Ophthalmological Society 2005     

中心性渗出性脉络膜视网膜病变的吲哚青绿造影特征

目的:分析中心性渗出性脉络膜视网膜病变(central exudative chorioretinopathy,CEC)吲哚青绿血管造影(indocyaninegreen angiography,ICGA)特点。方法:应用海德堡造影系统HRA2对24例24眼CEC患者进行荧光素、吲哚青绿眼底血管造影,并对ICGA与眼底荧光造影(fundus fluorescein angiography,FFA)结果进行对比分析。结果:CEC患者24眼FFA均证实为典型性脉络膜新生血管(choroidal neovascularization,CNV)。ICGA早期,24例CEC可见边界清晰的网状CNV。5例见CNV周围局限性脉络膜血管扩张。ICGA晚期可见CNV处染料渗漏或染色。20眼ICGA早期可见宽窄不一的暗环包绕CNV,4眼未见暗环。ICGA晚期,24眼CNV周围均可见暗环。1例患者光动力(photodynamic therapy,PDT)治疗后2mo,因视力继续下降复查ICGA,可见视网膜脉络膜血管吻合(retinal-choroidal anastomosis,RCA)。结论:由HRA2进行的ICGA对CEC的CNV的结构、边界、局部脉络膜静脉的改变、RCA的显示优于FFA。     

Polypoidal Choroidal Vasculopathy and Retinochoroidal Anastomosis in Japanese Patients Eligible for Photodynamic Therapy for Exudative Age-Related Macular Degeneration

Purpose

To determine the percentage of Japanese patients with age-related macular degeneration (AMD) who are eligible for photodynamic therapy (PDT) with verteporfin who have either polypoidal choroidal vasculopathy (PCV) or choroidal neovascularization (CNV) with retinochoroidal anastomosis (RCA).

Methods

The medical charts of 82 consecutive patients (83 eyes) with subfoveal CNV due to AMD were reviewed. Initially, we determined which of these eyes were eligible for PDT by using the criteria reported by two large randomized control studies, that is, the Treatment of Age-related Macular Degeneration with Photodynamic Therapy (TAP) study and the Verteporfin in Photodynamic Therapy (VIP) study. Among the PDT-eligible patients, the percentage of eyes with PCV or CNV with RCA was determined by indocyanine green angiography (ICGA).

Results

In total, 36 eyes (43%) of the 83 eyes were PDT-eligible; 17 (20%) based on the TAP study criteria, and 19 (23%) based on the VIP study criteria. Among these PDT-eligible eyes, ICGA revealed that 12 (33%) had PCV and 2 (6%) had CNV with RCA.

Conclusions

With ICGA, PCV or CNV with RCA were recognized in a substantial proportion of cases eligible for PDT based on the two clinical studies. Considering that the treatment efficacy of PDT for PCV or RCA has not been established, detection of PCV or RCA prior to PDT with ICGA is highly recommended.?Jpn J Ophthalmol 2006;50:354–360 © Japanese Ophthalmological Society 2006     

Effect of Quercetin on Formation of Choroidal Neovascularization (CNV) in Age-related Macular Degeneration(AMD)

Purpose: Age-related macular degeneration (AMD) as a disease entity is "dry" at early stage and made up of two main components at late stage:atrophic AMD and exudative AMD. Quercetin acts as an anti-oxidant to protect retinal pigment epithelial cells (RPE) from damaged by oxidative stress, but its effect on formation of choroidal neovascularization (CNV)in AMD is unclear. The aim of this study is to investigate the effect of quercetin on the formation of CNV in AMD. Methods:The development of CNV induced by laser was detected by fluorescein angiography (FA). Colored microsphere technique was used to determine the choroidal blood flow in ocular hypertensive rabbit eyes.In in vitro studies, HUVECs were treated with NaIO3, H2O2 and NaN3 to induce oxidative cell damages. The effect of quercetin on various oxidationsinduced injuries in HUVECs was measured by MTT assay. HUVECs migration was assessed using a wound healing assay. Results:Quercetin significantly inhibited the formation of laser-induced CNV.The choroidal blood flow in rabbit eyes was significantly increased after quercetin instillation. In vitro results showed quercetin enhanced various oxidations-induced injuries in HUVECs and inhibited migration of HUVECs during wound healing. Conclusion: Quercetin inhibited the formation of CNV both in vivo and in vitro and increased choroidal blood flow.It could become a promising candidate for the treatment of AMD.     

典型性脉络膜新生血管的眼底血管造影对比分析

目的 观察典型性脉络膜新生血管（CNV）的荧光素眼底血管造影（FFA）与吲哚青绿血管造影（ICGA）图像特征的异同。 方法 回顾分析34例（36只眼）典型性CNV患者的FFA和ICGA检查资料，并将FFA与ICGA结果进行对比分析。 结果 FFA显示典型性CNV的早期形态，在15只AMD患眼中有3只眼呈绒团状或车辐状轮廓，占20％；7只病理性近视患眼中有5只眼呈绒团状，占71.4％；14只中心性渗出性脉络膜视网膜病变中有9只眼呈绒团状，占64.3％。36只典型性CNV患眼，ICGA显示清楚的CNV20只眼，占55.6％；ICGA显示欠清楚的CNV15只眼，占41.6％；ICGA未能发现CNV的1只眼，占2.8％；ICGA可显示FFA不能显示的滋养血管6只眼，占16.7％。 结论 典型性CNV的FFA早期形态，AMD中多呈不规则形，而病理性近视和中心性渗出性脉络膜视网膜病变以绒团状居多。ICGA显示典型性CNV的轮廓边界不如FFA清楚，但可发现FFA显示不出的滋养血管。 (中华眼底病杂志, 2006, 22: 217-219)     

光动力联合TA后Tenon囊下注射治疗多灶性脉络膜炎合并CNV疗效观察

目的:评价光动力疗法(photodynamic therapy,PDT)联合曲安奈德(triamcinolone acetonide,TA)后Tenon囊下注射,治疗多灶性脉络膜炎(multifocal choroiditis,MC)合并脉络膜新生血管(choroidal neovascularization,CNV)的临床疗效。方法:回顾性分析8例8眼临床确诊为MC合并CNV患者的临床资料,对比分析PDT联合TA后Tenon囊下注射治疗前后患者最佳矫正视力(BCVA),FFA/ICGA和OCT等检查结果的变化。结果:联合治疗后随访6mo,所有患者BCVA与治疗前相比均明显提高,差异具有统计学意义(P<0.05)。FFA/ICGA检查显示,联合治疗后1moMC前节及眼底活动性炎症减退或停止者8眼(100%),CNV病灶渗漏停止或渗漏减少者7眼(88%)持续渗漏1眼(12%)。PDT治疗后6mo,MC炎症未发现复发,CNV病灶渗漏停止或渗漏减少者8眼(100%)。OCT显示CNV缩小,组织水肿减轻。结论:PDT联合TA后Tenon囊下注射治疗多灶性脉络膜炎合并脉络膜新生血管安全有效。     

Ultra-Widefield Indocyanine Green Angiography Reveals Patterns of Choroidal Venous Insufficiency Influencing Pachychoroid Disease

PurposeTo compare patterns of choroidal venous drainage in eyes with pachychoroid disease to those of healthy subjects using ultra-widefield indocyanine green angiography (UWF ICGA).MethodsPatients with pachychoroid disease and healthy controls were recruited at two referral centers. UWF ICGA images were used to evaluate the proportion of the postequatorial fundus drained by major vortex vein systems in each quadrant and to study the incidence and topography of choroidal vascular hyperpermeability (CVH) and intervortex venous anastomoses. Widefield swept-source optical coherence tomography (SS-OCT) was used to evaluate choroidal thickness at the posterior pole in eyes with pachychoroid disease.ResultsFifty-two pachychoroid eyes and 26 healthy eyes were evaluated. Eyes with pachychoroid disease showed a significant within-subject variance in the proportion of the postequatorial fundus drained by each vortex vein system (range, 4.1%–48.1%; P < 0.0001) that was not seen in controls (range, 17.3%–31.7%; P = 0.11). CVH was present in all pachychoroid disease eyes and three of 26 controls. Intervortex venous anastomoses were present in 46 of 52 pachychoroid disease eyes and nine of 26 control eyes. Vortex vein systems with large drainage areas showed greater density of CVH spots. SS-OCT demonstrated asymmetric choroidal drainage in the macula of 59% of pachychoroid eyes. CVH and intervortex venous anastomoses were more prominent in areas showing maximal choroidal thickness.ConclusionsIn eyes with pachychoroid disease, imbalanced choroidal venous drainage with congestion of specific vortex vein systems may contribute to a state of choroidal venous insufficiency characterized by regional choroidal thickening, CVH and remodeling of venous drainage routes.     

The Abnormal Choroidal Vessels in Aged Patients

Background:To show the abnormal choroidal vessels in aged patients with indocyanine-green angiography(ICGA).Methods:ICGA was performed in 350 patients with TOPCON TRC-50IA fundus camera.The images were recorded and retrospectively reviwed.Results:Five aged patients out of 350 cases were found to have abnormal choroidal vessels,The incidence was 1.43%,The abnormal choroidal vessels showed round-shapet,focal enlargement,abnormal shape and entrance,satellite appearance,and vascular loops.These might be due to congenital abnormality of choroid.ICGA could be used to observe the abnormal abnormal choridal vessels.     

Punctate Inner Choroidopathy and Choroidal Neovascularization in Korean Patients

ABSTRACT

Purpose: To describe the clinical features and outcomes of punctate inner choroidopathy (PIC) in Korean patients

Methods: We retrospectively reviewed the medical records of patients with PIC between 2004 and 2015. The main outcome measures included best-corrected visual acuity (BCVA), presence of choroidal neovascularization (CNV), and optical coherence tomography findings. Patients with and without CNV were compared.

Results: Forty eyes of 26 patients were included. The final BCVA was better than 20/40 in 33 eyes (82.5%). CNV was initially present in 12 eyes (30.0%). The mean initial and final logMAR BCVA was poorer for eyes with CNV than for eyes without CNV. Eyes with CNV exhibited a larger myopic refractive error, inflammatory lesions confined within the posterior pole, and a decreased final subfoveal choroidal thickness compared with eyes without CNV.

Conclusion: PIC in the Korean population generally exhibits favorable visual outcomes, and eyes with CNV show more abnormalities and poorer outcomes.     

Correlation Between Indocyanine Green Angiographic Findings and Histopathology of Polypoidal Choroidal Vasculopathy

Purpose To analyze the histopathology of polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV) developing from PCV, the authors evaluated correlations between pathological findings and the findings of preoperative indocyanine green angiography (IA).Methods Two specimens were obtained during CNV excision associated with PCV. PCV tissue was excised with the CNV. The specimens were examined by light microscopy.Results In one case, IA revealed polypoidal lesions exhibiting hyperfluorescence in both the early and the late phase, and in the affected area, abnormally dilated vessels were identified histologically underneath relatively healthy retinal pigment epithelium (RPE). In the other case, the polypoidal lesions seen on IA showed early hyperfluorescence and late isofluorescence, and dilated vessels were observed under the RPE; perivascular amorphous material was present. The RPE adhered to the side of the choroid, and there was CNV under the neurosensory retina in both cases. The CNV had numerous vascular lumens, was not surrounded by the RPE, and exhibited few fibrous components.Conclusions IA findings vary depending on the condition of the RPE located above the PCV and the extent of amorphous material around the PCV. Jpn J Ophthalmol 2004;48:249–255 © Japanese Ophthalmological Society 2004     

小鼠脉络膜血管新生模型中FLK-1的表达

目的:探讨小鼠脉络膜血管新生(choroidal neovascu larization, CNV)模型中FLK-1的表达.方法:采用激光击射的方法诱导成年C57BL/6小鼠CNV模型,术后行脉络膜灌流铺片及组织病理学检查观察CNV的发展.应用免疫组织化学染色探讨正常视网膜以及激光术后10d CNV模型中FLK-1的表达.结果:术后10d所有激光光斑均发展为实验性CNV,其中血管内皮细胞,类视网膜色素上皮细胞(retinal pigmented epithelium,RPE)及类成纤维细胞FLK-1表达强烈;在正常视网膜中,FLK-1仅在RPE、内核层及神经节细胞层有微弱的表达.结论:FLK-1参与了实验性CNV的发病过程,提示拮抗FLK-1可能成为防治CNV有效的生物学方法之一.