《植物检疫》征稿简则

《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物     

《植物检疫》征稿简则

《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检疫和病虫     

《植物检疫》征稿简则

《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检     

《植物检疫》征稿简则

正《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检疫和病     

《植物检疫》征稿简则

<正>《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检疫和病虫     

《植物检疫》征稿简则

《植物检疫》为国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检疫和病虫害疫情动态等信息;封面和封底刊登危险性有害生物的彩色图片。     

《植物检疫》征稿简则

<正>《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检     

《植物检疫》征稿简则

<正>《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查与监管的技术与方法,以及国际植物检疫和病     

《植物检疫》征稿简则

<正>《植物检疫》是国内外公开发行、全面反映国内外植物检疫的发展趋势和我国进出境植物检疫、农业植物检疫和森林植物检疫工作的技术性刊物。本刊主要刊登植物检疫和危险性有害生物的研究报告,有害生物检测鉴定、除害处理、产地检疫、调运检疫、疫情调查     

切实加强进出境木质包装检疫监管

近年来,进境货物木质包装成为携带林木有害生物在国际间传播的重要载体,正越来越受到世界许多国家的关注和重视,许多国家针对木质包装提出了严格的检疫措施,一方面对境外木质包装实行严格的检疫措施,保护本国的林木业;另一方面对出境的木包装按照输入国的要求实行除害处理,防止林木有害生物传出国门,南京检验检疫局多年来高度重视进出境木质包装检疫和除害处理,制定相关的管理办法和检疫措施,加强过程控制,强化检疫管理,采取多种切实有效办法,不断探索检疫监管新模式,收到了较好的效果.     

黄岩水果苗木与农产品检疫现状及对策建议

在1981-2018年黄岩水果类苗木及非种用农产品调运检疫中,共调出柑橘、杨梅和枇杷等苗木10 475批次,11 051.42万株;柑橘果实等非种用农产品147 689批次,852 920.95 t。基于柑橘等水果苗木调运检疫数量变化与水果产业发展变化关系,分析了果苗检疫存在的问题,包括无证育苗现象较普遍、检疫申报不规范、疫情信息不对称和漏检逃检等,提出了水果类苗木生产经营实行许可证制度、严格遵守植物检疫规程、建立种苗调运可追溯和到达地报告复检制度等建议,这对做好调运植物检疫工作有积极意义。     

柑橘溃疡病检疫与防治

细菌性溃疡病是严重危害世界柑橘产业的重大检疫性病害之一,柑橘溃疡病引起落叶、枯枝和落果,溃疡病斑导致果品质量降低,影响外贸出口。世界各国长期以来对病害采取严格苗木检疫、疫区病树铲除、零星病害药剂防控的综合治理措施;新近美国农业部推出"柑橘健康种植行动计划";2007年7月中国农业部正式启动"柑橘非疫区建设和维护"项目,总体目标在于防控柑橘溃疡病的发生和传播,确保柑橘产业的安全。     

Fenpropimorph: a promising fungicide for postharvest diseases in citrus fruits

Sour rot, green mold and blue mold are postharvest diseases of citrus fruit. Benzimidazole and imidazole fungicides control molds, and guazatine controls sour rot, but strains ofPenicillium spp. resistant to these chemicals have been reported. Aqueous formulations of fenpropimorph, a morpholine fungicide, are very active in controlling fungi attacking postharvest citrus fruits. The fungicidal effect of the chemical in the coating wax was investigated in combined treatments with guazatine in dip or spray applications on various citrus fruit varieties. Fenpropimorph alone was as good as or a better curative and protective treatment than the current commercial treatments applied in packinghouses in Israel for decay control.     

Citrus yellow mosaic badnavirus infecting <Emphasis Type="Italic">Citrus</Emphasis> sp.: a threat to the citrus industry and a quarantine issue

Among the virus diseases affecting the citrus industry in southern parts of India, the most devastation is caused by citrus tristeza virus (CTV) followed by citrus yellow mosaic badnavirus (CMBV). Research analysis reveals that the longevity of the trees, quality of fruit appearance, juice and ascorbic acid content are lowered in CMBV-infected citrus. CMBV is chiefly propagated through infected budwood, which is used in graft propagation of citrus species. Symptoms of the mosaic disease caused by the virus are always confused with those of mineral deficiencies and is the main reason for CMBV spread through graft propagation in nurseries. CMBV could also co-exist as a mixed infection with CTV. This mixed infection of CMBV and CTV causes even more devastation than CTV infection does alone. At present, this virus is limited to the southern parts of India. If CMBV is not properly diagnosed at the quarantine level, there is a high risk of virus movement to other citrus-growing countries of the world, especially for the border countries of India.     

柑桔溃疡病菌PCR快速检验检疫技术研究

 柑桔溃疡病是严重影响全世界柑桔生产的重大检疫性病害,根据柑桔溃疡病菌(Xanthomonas axonopodis pv. citri)新近公布的全基因组中独有的保守蛋白基因序列,设计筛选出一对种特异性引物(JYF5/JYR5),能专一地扩增检出柑桔组织表面所带溃疡病菌的DNA靶带(413 bp)。而柑桔叶面附生的非致病性黄单胞菌、野油菜黄单胞菌近缘种以及健康柑桔样品都不能扩增;靶细菌DNA检测下限1.56 pg/μL,靶细菌悬浮液检测下限10 cfu/μL;在不同PCR仪及各种控温方式下都能稳定地扩增出特征性靶带。这一特异、准确的柑桔溃疡病菌PCR检验技术和研制的预包被固相化PCR检测试剂盒已开始用于我国非疫生产区建设中柑桔苗木、果实的病害检疫检验。     

Potential distribution of citrus black spot in the United States based on climatic conditions

Citrus black spot (CBS), caused by Phyllosticta citricarpa McAlp Van der Aa, was recently detected in southern Florida in the US. In addition to infected plant propagation materials, movement of infected citrus fruit poses a concern for potential spread of the disease out of the current quarantine zone, because lesions with pycnidia and conidia could develop after harvest. The conditions conducive for mycelial growth and development of pycnidia and conidia are not well known. Therefore, effects of temperature and relative humidity on growth and conidial production of P. citricarpa were determined and used as parameter inputs in CLIMEX to predict potential establishment of CBS in North America. Colony growth and conidial production in vitro were optimal at 27 °C, whereas there was no growth below 4 °C and above 37 °C. On fruit, lesion development and conidial production were observed at 4 °C, though at a low rate, indicating a greater versatility of the fungus on fruit. More full pycnidia were produced on the CBS lesions at 91 % RH compared to 84 %. Input parameters for CBS risk in CLIMEX obtained from literature, which reflected conditions for infection in spring/summer in Florida, predicted potential establishment in Florida but not in California. However, altering the parameter values to account for survival of the pathogen in leaf litter in winter predicted potential establishment in California as well as Florida. Thus, P. citricarpa could possibly establish beyond Florida if this organism is transported outside of the current quarantine zone to other citrus production areas.     

寡毛实蝇的监测、鉴定和检疫处理研究

实蝇是水果和蔬菜的大害虫，在世界果蔬贸易中占有重要的地位。本研究采用国际通用的监测技术，利用Ｔｒｉｍｅｄｌｕｒｅ，Ｃｕｅｌｕｒｅ和Ｍｅｔｈｙｌｅｕｇｅｎｏｌ三种实蝇引诱剂于１９８０年开始至今，连续十多年对寡毛实蝇和地中海实蝇进行监测，同时也收集实蝇的寄主果实，开展周年季节性调查。经对收集的标本的鉴定，共发现寡毛实蝇２０种，其中有国内新记录６种。其详细描述收集在“实蝇及其防除”和“亚太地区寡毛实蝇”两本书中     

Citrus fruit flies of Sichuan Province (China)

The fruit fly pests on Sichuan citrus include Tetradacus citri and T. tsuneonis (Diptera: Trypetidae), regarded as distinct species in China. Special attention has been paid to the former, which is a quarantine pest in China. It is distributed in 18 counties of Sichuan Province and has caused serious losses in citrus orchards. Its hosts are all the citrus fruits, especially orange and pomelo. T. tsuneonis is distributed in only two counties and attacks only tangerine, but it is a potential hazard to all citrus crops in the province. Both species have only one generation per year. The pupae overwinter in soil. Emergence of T. citri begins at the end of April. Before oviposition the adults need supplementary nutrients. Twenty days after emergence, copulation begins and 15 days later the adult begins ovipositing (peak period mid-June to early July). The peak period for hatching is in early September. After three instars, the larva becomes mature in mid-October. The peak period of pupation is from the end of October to mid-November. Emergence of T. tsuneonis is about 15 days later than T. citri. The larvae bore the flesh of citrus fruits, causing great loss to citrus growers every year. Surveys are regularly conducted to check the distribution of the pests in Sichuan Province. Quarantine regulations enacted in 1982 and 1984 regulate the movements of articles that could spread the pests, with specified safeguards. Before female oviposition, spraying with trichlorfon in sugar solution gives satisfactory results in controlling citrus flies in orchards.     

柑橘鳞皮病研究进展

柑橘鳞皮病是一种分布在包括南美洲和地中海等广大地区的重要病毒性病害,随着大量国外优良柑橘品种的引进,增加了柑橘鳞皮病随同苗木、接穗传入我国的可能性。本文对柑橘鳞皮病的类型、研究历史、病原、传播方式、检测技术以及防治方法作一综述,为今后我国对柑橘鳞皮病的检疫和防治研究提供基础。     

Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp. citri under different treatments

Nucleic acid sequence based amplification (NASBA) is a method of amplifying RNA, for the detection of RNA viruses and human pathogenic bacteria. Recently, NASBA has also been employed for the detection of plant diseases caused by viruses and quarantine bacteria. A major citrus pathogen, Xanthomonas citri subsp. citri (Xcc), causal agent of citrus bacterial canker, is being studied in depth due to its economic importance, with recent focus concentrating on its viability and survival under different stress conditions and control treatments. In this work, a NASBA protocol using primers for gumD mRNA has been developed to assess the viability of this pathogen under different bacteriocidal treatments. This method is rapid, specific and sensitive, and is able to detect viable bacterial cells, using a hybridization device which allows the visualization of the results in only 30 min. The usefulness of the method has been confirmed with bacterial suspensions subjected to different heat treatments and to sodium orthophenylphenate.