乳宁外敷膏对家兔乳腺增生模型作用的实验研究

目的　观察乳宁外敷膏抑制和治疗乳腺增生病的效果。方法　选用健康雌性日本大耳白兔 30只 ,随机分成 6组 ,用己烯雌酚复制乳腺增生病模型。在制模过程中和制模成功后分别使用乳宁外敷膏和丙酸睾丸酮软膏外贴乳房部位 ,对照观察乳房外观形态、组织学形态和血浆中雌二醇的含量。结果　乳宁外敷膏能抑制兔乳腺增生 ,降低血浆中雌二醇含量。结论乳宁外敷膏对己烯雌酚所致的乳腺增生病有一定的治疗作用 ,可部分地使其恢复正常或减轻其增生程度 ,其作用效果大致与丙酸睾丸酮软膏相似     

乳贝洁片对家兔乳腺增生模型的影响

目的：通过观察乳贝洁片对家兔乳腺增生模型的影响,探讨该方的药效学作用,为临床治疗乳腺增生提供依据。方法：以雌二醇复制家兔乳腺增生模型,在造模成功后给家兔口服乳贝洁不同剂量,观察乳房的大体形态、病理组织学及血浆雌二醇的含量。结果：乳贝洁片明显抑制乳腺增生,同时可降低血浆中雌二醇含量。     

桂枝茯苓胶囊对大鼠乳腺增生病治疗作用的实验研究

目的:观察桂枝茯苓胶囊(桂枝、茯苓、牡丹皮、桃仁等)对实验性大鼠乳腺增生病的治疗作用,探讨其新疗效和作用机理.方法:本研究用肌注外源性苯甲酸雌二醇及黄体酮的方法复制大鼠乳腺增生模型,观察各组大鼠乳头形态学变化,用放射免疫方法测定各组实验性大鼠模型血浆内雌二醇(E2)、黄体酮(Pt)的含量,免疫组化方法观察乳腺组织中雌激素受体(ER)和孕激素受体(PR)的变化.结果:桂枝茯苓胶囊治疗组大鼠的乳头红肿、乳腺增大和显微小叶、腺泡、导管增生均显著轻于模型组;血液流变学指标、血浆雌二醇、乳腺组织中ER和PR含量显著低于模型组;血浆黄体酮含量则显著高于对照组.结论:桂枝茯苓胶囊对大鼠乳腺增生病有良好的治疗作用,其机理与改善血液流变、调节实验性大鼠性激素的血浓度及其受体的表达有关.     

乳癖消片治疗兔乳腺组织增生的形态学研究

目的:探讨乳癖消片治疗实验兔乳腺组织增生的疗效。方法:用苯甲酸雌二醇复制兔乳腺增生模型,分别饲喂乳癖消片和小金丸,观察实验兔乳房的大体形态和组织学形态变化。结果:乳癖消片能有效抑制乳腺导管上皮及腺沧上皮的增生。结论:乳癖消片对实验兔乳腺增生有明显的疗效。     

乳康酊的主要药效学研究

目的:观察乳康酊抑制乳腺增生及镇痛的作用。方法:采用大鼠苯甲酸雌二醇乳腺增生模型、热板及醋酸致痛等方法观察鼠乳头直径、血清雌二醇含量及痛阈组的变化。结果:乳康酊连续21 d对乳腺增生大鼠乳房皮肤给药,可明显减小乳头直径(P0.05)和降低血清雌二醇含量;升高小鼠热板致痛的痛阈值;延长小鼠醋酸致痛时间和减少扭体次数。结论:乳康酊具有抑制大鼠乳腺增生及镇痛作用。     

乳康酊的主要药效学研究

目的：观察乳康酊抑制乳腺增生及镇痛的作用。方法：采用大鼠苯甲酸雌二醇乳腺增生模型、热板及醋酸致痛等方法观察鼠乳头直径、血清雌二醇含量及痛阈组的变化。结果：乳康酊连续21 d对乳腺增生大鼠乳房皮肤给药,可明显减小乳头直径（P〈0.05）和降低血清雌二醇含量;升高小鼠热板致痛的痛阈值;延长小鼠醋酸致痛时间和减少扭体次数。结论：乳康酊具有抑制大鼠乳腺增生及镇痛作用。     

乳核速消胶囊对大鼠乳腺增生病治疗作用的实验研究

目的观察乳核速消胶囊对实验性大鼠乳腺增生病的治疗作用,探讨其疗效和作用机理。方法用肌注外源性苯甲酸雌二醇及黄体酮的方法复制大鼠乳腺增生模型,观察各组大鼠乳腺组织变化,用放射免疫法检测血清生殖激素三项(E2,P,PRL),免疫组化方法观察乳腺组织中雌激素受体(ER)和孕激素受体(PR)的变化。结果乳核速消胶囊治疗组大鼠的乳腺增大和腺泡、导管增生均显著轻于模型组;血浆雌二醇、乳腺组织中ER和PR含量显著低于模型组;血浆黄体酮含量则显著高于对照组。结论乳核速消胶囊对大鼠乳腺增生具有抑制作用,其机理与调节实验性大鼠性激素的血浓度及其受体的表达有关。     

鹿角胶抗乳腺增生的作用研究

目的:探讨鹿角胶对乳腺增生模型大鼠的治疗效果.方法:采用苯甲酸雌二醇联合黄体酮腹腔注射制备乳腺增生模型大鼠,观察鹿角胶对乳腺增生大鼠乳房直径和乳头高度变化,血清中孕酮(P)、黄体生成激素(LH)、促滤泡生成激素(FSH)、雌二醇(E2)含量变化及血液流变学指标的影响.结果:与模型对照组比较,鹿角胶能够明显调节血清中P、LH、FSH、E2的含量,改善血液流变学指标,降低乳腺增生大鼠乳房直径及乳头高度.结论:鹿角胶对乳腺增生模型大鼠具有一定的治疗作用.     

乳块消方对实验性乳腺增生大鼠的影响

目的：研究乳块消方(RKX)对实验性乳腺增生大鼠的影响,并探讨其作用机制。方法：60只大鼠随机分为乳块消方5.8,11.6,23.2 g·kg^-13个剂量组,三苯氧胺组,模型组和正常组。采用肌注苯甲酸雌二醇联合黄体酮的方法复制乳腺增生大鼠模型,造模同时给予药物治疗。测定乳房直径和乳头高度、脏器系数、血清性激素水平、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及血液流变性,镜下观察各组大鼠乳腺病理形态学变化。结果：乳块消方能明显减小大鼠乳房直径、乳头高度和乳腺小叶数、小叶内腺泡数,此外,明显降低血清雌二醇(E2)、催乳素(PRL)水平,提高孕酮(P)水平,增加SOD活性,抑制胸腺系数减少。结论：乳块消方对大鼠乳腺增生病有良好的治疗作用。     

小叶增生方外用对乳腺增生大鼠乳腺病理形态的影响

目的:研究小叶增生方外用对乳腺增生病模型大鼠乳腺病理形态的影响。方法:采用苯甲酸雌二醇序贯黄体酮肌肉注射建立乳腺增生病模型,模型建立后予外用中药贴敷第3对乳房,观察给药13 d后各组大鼠乳头直径及大鼠乳腺腺泡面积、导管内径的变化。结果:给药13 d后,给药组大鼠乳头直径缩小(P0.05),各组大鼠乳腺组织形态均恢复正常,各组比较,差异均无统计学意义(P0.05)。结论:小叶增生方外用能够促进乳腺增生病模型大鼠乳头直径缩小,但对乳腺病理形态未显示出调节作用。     

The anti-hyperplasia of mammary gland effect of Thladiantha dubia root ethanol extract in rats reduced by estrogen and progestogen

Aim of the study

Thladiantha dubia (Cucurbitaceae) has been used in Chinese manchu nationality folk medicine for treatment of various diseases, such as scrofula, carbuncle, lactation deficiency, etc. Its effects include softening hard masses, heat-clearing and detoxifying, reducing swelling, eliminating carbuncle, and promoting lactation. It has a history of use in the treatment of breast disorders and as an agent to increase lactation. The aim of the present study was to evaluate the anti-hyperplasia of mammary (HMG) effect of T. dubia root ethanol extract (TD-E) in estrogen combined with progestogen induced HMG rats.

Materials and methods

Forty virgin female Wistar rats were randomly divided into blank control, HMG model, Rupixiao Tablet (RPXT, positive control), and TD-E groups, 10 in each. Injection of estrogen and progestogen were given to establish rat models of HMG, RPXT and TD-E were given at the same time. Changes of nipple heights were measured; uterus and ovory index were calculated; sex hormone levels (LH, E₂, and P) in serum were estimated. Pathologic changes of MG in rats were also observed under light microscope.

Results and Conclusion

TD-E could decrease the increased nipple height, reduce the numbers of MG lobules and relieve the pathologic changes of HMG in rats. It could also decrease E₂ and LH levels in serum compared with the control group, nipple height, uterus index, and the levels of E₂ and LH in the HMG model group increased significantly, the level of P decreased significantly. Compared with the HMG model group, nipple height, uterus index, and the levels of E₂ and LH in the TD-E group decreased, and no obvious effect on P content. The histopathological analysis suggested that TD-E obviously alleviated the degree of HMG. Those results suggest TD-E has protective and therapeutic effects on HMG rats induced by estrogen and progestogen.     

Efficacy of moxibustion by stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) on hyperplasia of mammary gland in rats

OBJECTIVE: To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) in rats with hyperplasia of mammary gland(HMG) which induced by estrogen and progestogen.METHODS: Thirty female Sprague-Dawley rats were randomly divided into saline control group,HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progesto-gen with moxibustion at the same time. The Changes of nipple diameter and height were measured.The rats' skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.RESULTS: The diameter and height of the nipples in model group were prominently bigger and higher than that in control group(P 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group(P 0.01), and there was no significant difference between moxibustion group and control group.Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong(CV 17) and Ganshu(BL 18) decreased prominently in model group(P 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently(P 0.05).CONCLUSION: Treatment with moxibustion can effectively decrease the HMG rats' nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia.     

大鼠乳腺癌癌前病变肝郁证造模的研究

目的:通过大鼠乳腺癌癌前病变结合肝郁证造模研究,观察大鼠在癌前病变病证结合造模阶段生物学行为、激素水平、组织病理学的改变,探讨成模规律,健全造模评价指标。方法:实验大鼠随机分为空白对照组、二甲基苯蒽造模组、雌二醇造模组、二甲基苯蒽加夹尾造模组、雌二醇加夹尾造模组,采用二甲基苯蒽灌胃复制乳腺癌癌前病变大鼠模型,苯甲酸雌二醇和黄体酮肌注复制乳腺增生大鼠模型,夹尾激惹法复制肝郁证模型。观察各时期大鼠一般情况、5-羟色胺检测、组织病理学的改变。结果:①病证结合造模两组大鼠与单纯疾病造模两组大鼠比较,一般行为更符合肝郁证特点(P0.01),5-羟色胺检测有显著差异(P0.01);②在组织形态方面,第8周二甲基苯蒽加夹尾造模组癌前病变出现率明显高于二甲基苯蒽造模组(P0.05),第12周二甲基苯蒽加夹尾造模组与二甲基苯蒽造模组癌前病变和肿瘤出现率无显著差异(P0.05),但均明显高于雌二醇造模组和雌二醇加夹尾造模组(P0.01)。结论:二甲基苯蒽灌胃加夹尾激惹能成功复制大鼠乳腺癌癌前病变肝郁证模型。该模型大鼠的一般行为、神经内分泌表现符合中医肝郁证特点。这种病证结合造模方法能缩短癌前病变动物造模时间,提高造模成功率。     

Anorectic and behavioural effects of chronic Cissampelos sympodialis treatment in female and male rats

Male and female rats were treated daily for 13 weeks with an ethanol extract of Cissampelos sympodialis leaves (9, 45 and 225 mg[sol ]kg). The food consumption, body weight and behavioural effects in the open-field test were evaluated by weekly monitoring. The results showed that the extract chronic treatment in female rats (45 and 225 mg[sol ]kg) reduced significantly the food intake and the body weight, and produced several alterations in the open-field test. These findings indicate that repeated oral administration of the extract may produce a sex-dependent difference in anoretic and behavioural effects.     

消瘀通络散对乳腺增生大鼠乳腺组织形态与内分泌的影响

目的:观察自拟消瘀通络散对乳腺增生大鼠乳头直径、高度,血清E2/P、PRL、T水平以及乳腺组织形态的影响,探讨其治疗乳腺增生的作用机理。方法:将40只Wistar大鼠随机分为正常组、模型组、中药组和西药组4组。采用己烯雌酚联合黄体酮肌注的方法进行造模。中药组给予自拟消瘀通络散50mg/d,西药组予三苯氧胺1.8mg/kg,均灌胃治疗,1次/d,共30d。于治疗前后测量各组大鼠第2对乳头直径、高度,测定血清E2、P、PRL、T水平,计算E2/P数值,取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现。结果:治疗后乳头直径、高度比较,中药组和西药组均低于模型组(P0.05);2组之间比较,中药组低于西药组(P0.05)。血清各激素水平比较,中药组和西药组血清E2/P、PRL、T含量均低于模型组(P0.05);中药组与西药组相比无显著性差异(P0.05)。病理学比较,中药组和西药组大鼠乳腺组织病理表现相似,与模型组比较改善明显,与正常组相近。结论:自拟消瘀通络散具有缩小模型大鼠乳头直径、高度,改善内分泌紊乱状态和乳腺组织病理形态的作用。     

The inhibitory effect of bamboo extract on the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer

The incidence of breast cancer among women is high and increasing. This study investigated the inhibitory effect of an extract from bamboo Phyllostachys edulis on the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in female Sprague-Dawley rats. The rats were fed with bamboo extract (BEX) supplemented diet or control diet, and treated with DMBA after 3 weeks of the dietary regime. The incidence of mammary tumors was monitored by palpation for the next 11 weeks. At the end of the experiment, blood samples were collected for total antioxidant capacities (TAC) assay and liver samples for phase II enzyme activity assays. The TAC values, total contents of phenolics and flavonoids of BEX were also measured. The results showed that BEX delayed the onset of mammary tumor by 1 week, decreased the tumor incidence by 44% and tumor multiplicity by 67%, and increased the total sulfotransferases (SULT) activity by 63%. BEX showed high levels of TAC, total phenolic and total flavonoids. However, the serum TAC values were not affected by BEX supplementation. In summary, the results indicate that BEX possesses a potent anti-breast cancer effect, and the upregulation of SULT activity, therefore estrogen metabolism may be the underlying mechanism.     

血府逐瘀胶囊对流产大鼠血瘀型产后恶露不绝治疗作用研究

[目的]研究血府逐瘀胶囊对流产大鼠血瘀型产后恶露不绝的治疗作用。[方法]将实验动物按体质量随机分为正常对照组、模型对照组、阳性药对照组、血府逐瘀高、中、低剂量组。雌雄按2∶1比例合笼,雌鼠受孕第7天分别灌胃米非司酮和米索前列醇,复制大鼠早孕流产模型,造模后给予受试药7 d,测定血流变,血小板聚集率、血红蛋白含量、红细胞数目、子宫病理形态学,免疫组化法测定子宫微血管密度(MVD)。[结果]血府逐瘀胶囊高、中剂量组可以显著降低产后血瘀大鼠血小板聚集率(P0.05,P0.01),改善血流变学相关指标,降低全血及血浆黏度(P0.05,P0.01),增加血瘀大鼠的红细胞(RBC)及血红蛋白(HGB)含量(P0.05,P0.01)。改善模型大鼠生存状态、子宫形态,提高子宫MVD,促进血管再生。[结论]血府逐瘀胶囊对产后血瘀所致恶露不绝的大鼠有较为明显的治疗作用。     

健乳灵治疗乳腺增生病临床和实验研究

目的：探讨健乳灵治疗乳腺增生病的疗效和对性激素的调节机理。方法：观察健乳灵治疗前后乳腺增生病的临床变化及血清性激素变化。动物实验通过病理，电镜，放射免疫等法观察健乳灵治疗乳腺增生病的疗效和性激素的变化。结果：健乳灵治疗乳腺增生病，治疗组临床治愈率为７２５％，总有效率为９０．８％，与对照组比较有显著性差异，而且有调节性激素水平的作用；雌二醇和垂体催乳素明显降低；而孕酮和睾酮则有回升；     

柴瓜消结汤对实验性乳腺增生家兔性激素水平的影响

目的:探讨柴瓜消结汤治疗乳腺增生病的疗效和对性激素的调节作用.方法:通过动物实验观察柴瓜消结汤治疗乳腺增生病的疗效和性激素的变化.结果:家兔乳腺高度及乳腺直径均较模型对照组有明显缩小;雌二醇(E2)和垂体催乳素(PRL)明显降低(均P＜0.05),孕酮(P)和睾酮(T)则有回升(均P＜0.05),促卵泡成熟激素(FSH)和促黄体生成素(LH)改变无显著性差异(均P＞0.05).柴瓜消结汤对实验性增生家兔有明显防治作用,能调整家兔体内的性激素分泌.结论:柴瓜消结汤治疗乳腺增生病有显著的临床疗效,并能调整机体内源性激素水平.     

紫苏梗对大鼠离体结肠肌条收缩运动的影响

[目的]观察紫苏梗水提液对正常及肢体缺血-再灌注大鼠结肠环形肌条收缩波平均振幅和张力的影响。[方法]利用肢体缺血-再灌注大鼠制备胃肠动力障碍(FD)模型。取大鼠结肠环形肌条,置于恒温灌流肌槽内,通过多导生理记录仪记录紫苏梗在结肠环形肌条收缩活动中的作用。[结果]紫苏梗水提液能升高FD模型大鼠结肠环形肌条收缩波平均振幅、降低结肠张力,对正常大鼠则无明显影响。[结论]紫苏梗水提液对FD模型大鼠结肠环形肌条收缩运动具有明显兴奋作用,而对正常大鼠结肠收缩运动无兴奋作用。