胃癌微卫星DNA不稳定性和hMSH2 mRNA的表达

目的探讨胃癌微卫星DNA不稳定性与错配修复基因hMSH2 mRNA表达之间的关系. 方法采用PCR-变性聚丙烯酰胺凝胶电泳-银染技术,对20例胃癌及其癌旁组织D9s171、D9s1604微卫星位点杂合性缺失(LOH)进行分析,原位杂交检测27例胃癌、10例癌旁组织和19例正常胃黏膜中hMSH2 mRNA的表达.结果胃癌D9s171、D9s1604微卫星位点LOH发生率(13/20)较其癌旁组织(6/20)明显增高(P<0.05);两位点与LOH发生之间存在相关性(P<0.05).hMSH2 mRNA阳性表达细胞在胃癌及其癌旁组织中为(42.1±25.9)和(99.7±16.8),较正常标本的(175.8±26.4)明显减少;不同分化程度胃癌组织间hMSH2 mRNA表达阳性率不同, 胃癌组织LOH(+)标本hMSH2 mRNA阳性表达(25.5±10.7)较LOH(-)者(61.2±25.9)显著降低(P<0.05).结论 D9s171、 D9s1604微卫星位点的LOH与胃癌的发生发展有关,胃癌中微卫星DNA不稳定性的产生可能是DNA错配修复基因hMSH2 mRNA低表达所致.     

胃癌组织p16基因微卫星DNA的不稳定性

目的：检测胃癌组织中p16基因D9s171、D9s1604微卫星位点杂合性缺失（LOH）,探讨p16基因微卫星不稳定性（MI）与胃癌发生发展的关系。方法：采用PCR－变性聚丙烯酰胺凝胶电泳－银染技术对20例胃癌和癌旁组织LOH进行分析。结果：癌组织D9s171和D9s1604的LOH分别为3例和10例,癌旁组织分别为2例和4例。两位点LOH发生率差异无显著性（P＞0．05）。胃癌组织较癌旁组织LOH发生率明显增高（P＜0．05）;早期胃癌与进展期胃癌两个微卫星位点LOH的发生率分别为50％（4／8）和58．3％（7／12）（P＞0．05）;两位点LOH发生存在相关性（P＜0．05）。结论：D9s171,D9s1604微卫星位点的LOH与胃癌的发生发展有关,而与癌细胞的分化程度或胃癌的临床分期无显著相关性。     

胃癌组织p16基因微卫星DNA的不稳定性

目的 :检测胃癌组织中p16基因D9s171、D9s16 0 4微卫星位点杂合性缺失 (LOH) ,探讨p16基因微卫星不稳定性 (MI)与胃癌发生发展的关系。方法 :采用PCR 变性聚丙烯酰胺凝胶电泳 银染技术对 2 0例胃癌和癌旁组织LOH进行分析。结果 :癌组织D9s171和D9s16 0 4的LOH分别为 3例和 10例 ,癌旁组织分别为 2例和 4例。两位点LOH发生率差异无显著性 (P >0 .0 5 )。胃癌组织较癌旁组织LOH发生率明显增高 (P <0 .0 5 ) ;早期胃癌与进展期胃癌两个微卫星位点LOH的发生率分别为 5 0 % (4/ 8)和 5 8.3% (7/ 12 ) (P >0 0 5 ) ;两位点LOH发生存在相关性(P <0 0 5 )。结论 :D9s171,D9s16 0 4微卫星位点的LOH与胃癌的发生发展有关 ,而与癌细胞的分化程度或胃癌的临床分期无显著相关性     

胃癌组织中错配修复基因hMSH2 mRNA的表达

目的 :研究hMSH2mRNA在胃癌、癌旁及正常胃粘膜组织中的表达。方法 :应用原位杂交技术 ,检测了2 7例胃癌、10例癌旁组织和 19例正常胃粘膜组织中hMSH2mRNA的表达。计数阳性细胞数。结果 :胃癌、癌旁组织与正常胃粘膜组织hMSH2mRNA原位杂交阳性率分别为 74 1% ,6 0 0 % ,6 8 4%。胃癌杂交阳性细胞数 (42 1±2 5 9) ,较癌旁 (99 7± 16 8)与正常组织 (175 8± 2 6 4)显著减少 (P <0 0 1) ;不同临床分期胃癌组织间阳性细胞数差异无显著性 (P >0 0 5 )。结论 :DNA错配修复基因hMSH2mRNA低表达可能导致基因组不稳定性进而诱发肿瘤     

DNA错配修复基因hMSH2蛋白表达与胃癌发生关系的探讨

[目的]探讨DNA错配修复基因hMSH2蛋白表达与胃癌发生以及不同病理类型之间的关系.[方法]应用免疫组化技术检测hMSH2基因在38例胃癌患者的癌组织及其癌旁组织的蛋白表达.[结果]癌细胞阳性表达率(52.63%)明显高于癌旁上皮细胞(23.68%)(P＜0.05);同例中癌细胞和癌旁上皮细胞均表达的为15.79%,同例癌细胞表达而癌旁上皮细胞不表达的例数(14/38)明显多于癌旁上皮细胞表达而癌细胞不表达的例数(3/38)(P＜0.05);高中分化腺癌、低分化腺癌和粘液癌表达阳性率分别为33.33%、52.63%、和70%.[结论]DNA错配修复基因hMSH2蛋白表达与胃癌发生密切相关.     

不同分化程度胃癌组织中hMSH2 mRNA的表达

目的 :研究正常胃粘膜和不同分化程度胃癌胃粘膜组织中hMSH2mRNA的表达。方法 :应用原位杂交技术 ,对 2 7例胃癌 (高、中、低分化分别为 5例 ,9例与 13例 )和 19例正常胃粘膜组织中hMSH2mRNA表达进行检测。结果 :癌组织中杂交信号阳性细胞数与正常组织相比显著减少 (P <0 0 5 )。低分化胃癌 (2 1.8± 10 .6 )与中分化胃癌 (5 0 .9± 19.3)及高分化胃癌 (72 .5± 2 3.3)相比差异显著 (P <0 .0 1)。结论 :hMSH2mRNA表达与胃癌分化程度有关 ,胃癌细胞的分化程度愈低 ,其hMSH2mRNA表达愈低。hMSH2可能对胃癌细胞存在分化诱导或转录水平调控     

死亡相关蛋白激酶基因在胃癌组织中的表达

目的:研究死亡相关蛋白激酶(DAPk)基因在原发性胃癌组织中的表达,探讨其在胃癌发生发展中可能的作用。方法:收集62例原发性胃癌及相对应的癌旁正常组织。应用RT-PCR检测DAPk mRNA表达,免疫组化方法检测DAPk蛋白表达。结果:RT-PCR方法显示DAPk mRNA表达水平在胃癌组织中明显低于癌旁正常组织(OD值为0.2863±0.2027比0.5736±0.1968,P<0.0001)。免疫组化分析显示,DAPk在所有癌旁正常组织中表达均为阳性,积分范围为2-12,平均8.61±2.89;而在胃癌组织中表达则明显减弱或缺失,积分范围为0-9,平均2.90±3.38。DAPk在癌旁正常组织中的表达水平显著高于胃癌组织(P<0.0001)。结论:在原发性胃癌组织中,DAPk mRNA和蛋白表达明显缺失或低下。     

乳腺癌3p染色体微卫星杂合性缺失与血管生成

目的探讨乳腺癌染色体3p区域微卫星杂合性缺失(LOH)与血管内皮生长因子(VEGF)蛋白表达及临床病理特征之间的关系。方法选取乳腺浸润性导管癌病例石蜡组织切块40例。运用激光显微切割技术留取组织中导管癌及正常乳腺细胞,应用毛细管电泳测序技术进行4个微卫星位点的LOH检测。应用免疫组织化学技术SP法检测CD34、VEGF和Her2的表达,分析LOH与乳腺癌临床病理参数的关系。结果 40例乳腺癌患者中D3S1038的LOH发生率为20%(8/40),D3S1295的LOH发生率为37.5%(15/40),D3S1581的LOH发生率为17.5%(7/40),D3S3118的LOH发生率为5%(2/40)。LOH与VEGF的表达呈正相关(r=0.658 0,P<0.01),LOH和MVD也呈正相关(r=0.804 9,P<0.01)。LOH发生率与Her2表达(r=0.539 4)、组织学分级(r=0.497 2)、淋巴结转移(r=0.506 4)、TNM分期(r=0.596 0)均有相关性(均P<0.01)。结论乳腺癌染色体3p微卫星位点LOH与血管生成具有相关性,抑癌基因失活并通过对血管生成的影响可能为肿瘤生长与转移提供了条件;同时检测染色体3p微卫星位点LOH与血管生成情况,可能有助于更全面地了解乳腺癌的生物学特性。     

错配修复基因及微卫星DNA与B细胞非霍奇金淋巴瘤发病机制的关系研究

[摘要] 目的 探讨DNA错配修复基因系统(Mismatch repair gene system, MMR)功能缺陷及微卫星不稳定性( Microsatellite instability, MSI)在B细胞非霍奇金淋巴瘤（Non-Hodgkins lymphoma, NHL）发病机制中的作用。方法 对42例B细胞NHL肿瘤组织,运用免疫组织化学SP法检测hMSH2、hMLH1 蛋白表达情况并采用PCR技术检测4个微卫星位点D17S945、D17S938、D17S947、D17S926的微卫星不稳定性。结果 42例B细胞NHL的肿瘤组织中,hMSH2、 hMLH1蛋白表达缺失率分别为33.33%（14/42）、38.10%（16/42）,MSI阳性率为30.95%(13/42)。hMSH2、hMLH1 蛋白表达缺失及MSI与肿瘤是否发生于淋巴结无关。结论 DNA错配修复系统基因缺陷及微卫星不稳定性在B细胞NHL的发病机制中的作用有限。     

宫颈鳞癌和食管鳞癌共同相关的抑癌基因的研究

目的 比较食管鳞癌和宫颈鳞癌组织中9号染色体相同区域相同位点等位基因杂合性缺失(LOH)率,探索与二者共同相关的抑癌基因.方法 采用显微切割、PCR、变性聚丙烯酰胺凝胶电泳、AgNO3,染色等技术,检测45例食管鳞癌和46例宫颈鳞癌组织中的9号染色体上7个微卫星位点的LOH.结果 食管鳞癌总LOH率为25.8%(55/213),7个微卫星位点的LOH率从大到小依次为D9S753(42.9%)、D9S303(38.5%)、D9S171(35.7%)、D9S162(36.4%)、D9S242(16.7%)、D9S1748(16.7%)、D9S43(0).宫颈鳞癌中总LOH率为25.2%(54/214),7个微卫星位点的LOH率从大到小依次为D9S171(40.7%)、D9S43(33.3%)、D9S162(3114%)、D9S242(23.7%)、D9s303(17.2%)、D9s753(16.7%)、D9S1748(12.9%).结论 食管鳞癌和官颈鳞癌中存在相同的高频率的LOH位点,9号染色体上可能存在与二者发生发展共同相关的抑癌基因.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.