Compositional differences,antioxidant activity,angiotensin-converting enzyme inhibition,and sensory characteristics of kamaboko fermented in wheat,rice, brown rice,and soybean koji

Fisheries Science - Fermentation of kamaboko in koji with wheat (KW), rice (KR), brown rice (KB), or soybean (KS) was performed by simulation of tofuyo processing. Moisture and protein content...     

Prevalence of nematode parasite,Contracaecum, in Nile tilapia,African catfish and Barbus species in Lake Hawassa,Ethiopia

A cross‐sectional study was conducted from November 2017 to March 2018 at Lake Hawassa to determine the prevalence of larval Contracaecum infestation in Nile tilapia, (Oreochromis niloticus), African Catfish (Clarias gariepinus) and Barbus species (Barbus intermedius). Fifty‐two point six per cent (52.6%) previous prevalence and 5% precision were used to estimate the sample size. Accordingly, a total of 383 randomly sampled fish species comprising of 163 (42.6%) C. gariepinus, 159 (41.5%) O. niloticus and 61(15.9%) B. intermedius were examined. The length and weight of each sampled fish were recorded. All the sampled fish were examined for the evidence of Contracaecum parasites in Hawassa University Veterinary Parasitology and Pathology Laboratory. Chi‐square values and comparison of proportions were used to analyse the data. The overall prevalence of Contracaecum parasites of fish population was 31.6%. The distribution of parasite was significantly affected by fish species (p = .000), sexes (p = .018), length (p = .003) and weight classes of fish (p = .026). As a hygienic problem and gutting activity conducted at the sides of the Lake Hawassa and distribution of discarded fish wastes for surrounding piscivorous birds by butchers and other people, the life cycle of Contracaecum parasite was perpetuated. Thus, the parasite is of zoonotic significance. Therefore, awareness creation activities for societies and control of fish parasites should be conducted in the study area.     

青岛胶州湾铠平鲉年龄、生长和种群繁殖力

针对2013年和2014年采自青岛胶州湾的209尾铠平鲉(Sebastes hubbsi)样本开展了年龄、生长和种群繁殖力研究。耳石轮纹结构的年龄鉴定结果显示,铠平鲉的年龄分布范围为1~+~7~+龄,2~+~4~+龄为优势年龄组(77.5%)。体长与体重呈幂函数关系:W=8×10~(-5) L~(2.797)(R~2=0.861)。Von Bertalanffy体长生长方程为L_t=131.4×[1-e~(-0.269(t+2.697))],体重生长方程为W_t=63.6×[1-~(e-0.269(t+2.697))]~(2.797)。其生长过程可分为3个阶段:(1)1~+~3~+龄为快速生长期;(2)3~+~6~+龄为成熟稳定期;(3)6~+龄以后为高龄衰老期。所采样本的性比(2.12︰1)明显偏离1︰1(P0.05),雌性个体样本数在各体长段均高于雄性个体。性腺指数(GSI)和肝脏指数(HSI)变化趋势相反,肝脏可能为其性腺发育提供物质基础和能量来源。绝对怀卵量波动于482~6166粒,平均值为3266粒;相对体长怀卵量显著低于卵生鱼类,表明卵胎生鱼类体内胚胎发育、孵化的护卵方式可能使其以较少的怀卵量即可维持种的延续。结合拐点年龄对应的拐点体重及50%性成熟体长,建议以体长80 mm、体重20 g作为铠平鲉的开捕标准,以维护青岛胶州湾铠平鲉资源的可持续利用。     

Effect of Stocking Density on Growth,Yield, and Costs of Producing Rainbow Trout,Oncorhynchus mykiss,in Cages

Abstract

Rainbow trout, Oncorhynchus mykiss, were raised in culture cages (1 m³) to determine the effect of stocking density on growth, survival, and percentage of market-size fish. Large fingerling rainbow trout (20-25 cm, 232 g average weight) were stocked into six cages located in a 0.4-ha pond. Two stocking densities (100 or 200 fish/cage) were used, and fish were grown for 140 days (2000-April 2001). Average total harvest weight (35.0 kg) in the low-density cages was approximately one-half the average total harvest weight (61.2 kg) in the high-density cages. Average weight gain (11.7 kg to 15.1 kg) and feed conversion (1.2 to 1.5) were also smaller for the low-density cages. Average survival was 96.7% for the low-density cages and 94.2% for the high-density cages, with the percentage of market-size fish (< 29 cm) averaging 50.3% and 52.0%, respectively. Production costs for the actual experiment and the revenues from fish sold at the end of the study were collected. An enterprise budget based on the experimental results for the two densities was developed to determine if a culture operation of this size would produce a net return. Production costs and revenues from the experiment resulted in a large negative return (-$3,124) and high breakeven price ($13.53/kg).     

Apparent Digestibility Coefficients and Amino Acid Availability of Cricket Meal,Gryllus bimaculatus,and Fishmeal in African Catfish,Clarias gariepinus,Diet

A study was conducted on African catfish, Clarias gariepinus, to examine the apparent digestibility coefficient (ADC) of protein, dry matter, lipid, and energy, and the apparent availability coefficient (AAC) of essential amino acid (EAA) for a cricket meal (CM) diet, Gryllus bimaculatus, and fishmeal (FM). Both diets consisted of 30% isonitrogenous levels with a 70:30 ratio of basal diet to test ingredient. Chromic oxide (0.5%) was added to the diet as an inert marker. The ADCs of crude protein (CP) in CM (81.21%), lipid (89.82%), gross energy (64.42%), and dry matter (73.97%) was significantly higher (P < 0.05) than the value of CP (78.22%), lipid (82.03%), gross energy (56.52%), and dry matter (62.26%) in FM. All EAAs were present in both diets. The AACs for both diets ranged from 0.906 to 0.961 and 0.812 to 0.938 for the CM and FM diets, respectively. The growth performance of fish fed with CM exhibited significantly higher (P < 0.05) weight gain, feed conversion ratio, and protein efficiency ratio compared with FM. Overall, the results suggest that CM could be included in the African catfish diet without affecting their nutrient and amino acid digestibility as well as growth performance.     

Sperm Motilities in Waigieu Seaperch,Psammoperca waigiensis: Effects of Various Dilutions,pH, Temperature,Osmolality, and Cations

The objectives of this study were to evaluate the effects of varying dilutions, pH, temperature, osmolality, and cations on sperm motility parameters in waigieu seaperch, Psammoperca waigiensis. The maximum velocity of average in path (VAP), percentage of motile cells (MOT), and duration of sperm motility (DSM) were observed when semen was diluted in artificial seawater (ASW) at a ratio of 1:100 (144.9 ± 0.6 µm/sec, 95.6 ± 0.4%, and 230.3 ± 2.3 sec, respectively), at 30 C (142.0 ± 0.6 µm/sec, 93.6 ± 0.4%, and 238.3 ± 0.9 sec, respectively), and pH 8 (144.8 ± 0.6 µm/sec, 93.3 ± 0.4%, and 234.0 ± 1.5 sec, respectively). Maximum VAP, MOT, and DSM were obtained in each solution containing 0.6 M NaCl (143.8 ± 1.0 µm/sec, 91.3 ± 2.0%, and 230.6 ± 4.2 sec, respectively), 0.6 M KCl (135.1 ± 3.1 µm/sec, 91.1 ± 3.1%, and 230.3 ± 3.7sec, respectively), 0.2M CaCl₂ (105.3 ± 4.7μm/sec, 47.9 ± 2.7%, and 120.7 ± 1.3 sec, respectively), 0.2 M MgCl₂ (107.3 ± 3.0 m/s, 42.1 ± 3.3%, and 120.3 ± 4.8 sec, respectively), and osmolality of 400 mOsm/kg (145.1 ± 2.5 µm/sec, 93.0 ± 2.1%, and 346.5 ± 4.4s, respectively). We used these mediums as artificial insemination media for fertilizing matured eggs. The results showed that the fertilization and hatching rates in 0.6 M NaCl (75.3 ± 0.6% and 57.0 ± 2.4%, respectively), ASW (70.8 ± 1.2% and 51.2 ± 1.8%, respectively), or 400 mOsm/kg (72.9 ± 1.8% and 55.3 ± 1.6%, respectively) were higher than that in seawater (63.9 ± 1.2% and 39.2 ± 3.9%, respectively). In conclusion, using 0.6 M NaCl, ASW, or 400 mOsm/kg as an artificial insemination medium is effective for fertilizing of waigieu seaperch.     

Validation,use, and disadvantages of enzyme-linked immunosorbent assay kits for detection of cortisol in channel catfish,largemouth bass,red pacu,and golden shiners

Measurement of cortisol response is an important tool to asses stress in fisheries research. Radioimmunoassay (RIA) is a common method for the measure of cortisol in fish. Use of enzyme-linked immunosorbent assays (ELISA) to detect cortisol would eliminate health hazards, costs of handling radioisotopes, and the short stability time associated with RIA. Enzyme-linked immunosorbent assays have been used for the determination of cortisol in several fish species. However, the ELISA method of cortisol determination in fish lacks proper validation testing. We conducted validation procedures for multiple commercial cortisol ELISA kits and compared the results to RIA. The assays were tested for four species: (1) channel catfish Ictalurus punctatus, (2) largemouth bass Micropterus salmoides, (3) red pacu Piaractus brachypomus, and (4) golden shiners Notemigonus crysoleucas. We evaluated the ELISA methods against RIA, and determined that at least one kit is suitable (accuracy: mean recovery of spiked samples, 102.8%; reproducibility: interassay coefficient of variation < 10.5% for all species; precision: intra-assay coefficient of variation < 16.8% for all species; linearity: R ² > 0.96 for all species) for the measurement of cortisol response in fish and comparative determination of stress. All of the ELISA assay results varied by more than 10% from the cortisol concentrations detected by the RIA. The high variability of the kit results indicates that commercial ELISA kits could be utilized for qualitative determination of cortisol in fish, but should be fully validated in each laboratory for each species before being used for research.     

Presence and distribution of PAHs,PCBs and DDE in feed and sediments under salmon aquaculture cages in the Bay of Fundy,New Brunswick,Canada

• 1. Wastes from feed and faeces can result in the deposition of contaminants in sediments around aquaculture sites. Five types of feed pellet, a commercial fish oil and 76 sediment samples collected under and up to 100 m away from salmon aquaculture cages in the south‐western Bay of Fundy between 1998 and 2000 were analysed for polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and chlorinated pesticides.
• 2. Five alkylated naphthalenes (aNAs) were consistently detected in fish oil (116–180 ng g^?1, per aNA), in pellets (25–51 ng g^?1, dry weight, per aNA), and sediments (<1–45 ng g^?1, dry weight, per aNA). Other PAHs were detected at variable levels in feed or in sediments.
• 3. IUPAC congener 153 (a PCB) and p,p′‐dichlorodiphenyldichloroethylene (p,p′‐DDE) were detected more frequently than other chlorinated targets in oil (108 ng g^?1 and 176 ng g^?1 respectively), feed (<1–14 ng g^?1 and 1.7–28.2 ng g^?1 respectively) and sediments (<0.08–3 ng g^?1 and <0.5–7 ng g^?1 respectively).
• 4. Trends were observed during the first year of sediment sampling (1998), with higher organic carbon, PCBs and p,p′‐DDE levels below the cages than 50 m away. The PAHs other than aNA showed an opposite trend with distance. In 1999, levels of p,p′‐DDE and PCBs were somewhat reduced under the cages, but were detected up to 100 m away from the cages.
• 5. Levels of aNA tended to be higher at some sites in 1999 relative to 1998, reflecting variability in feed and/or differential transport or degradation of contaminants relative to the environmental background. When detected, mean levels of PCBs and DDE were 2–10 times lower in 2000 than in 1999, and PAHs were 30–40% lower in 2000 than in 1999.
• 6. Interpretation of the results is done cautiously, since the exact locations of the sites sampled is only known to an intermediate facilitating the work between farmers and scientists. Levels of contaminants are compared with international results, interpreted relative to potential toxicity, uptake from feed, from deposited sediments, and in the framework of an aquaculture decision‐support system published recently.

Copyright © 2005 John Wiley & Sons, Ltd.     

Isolation, cloning, sequencing of brain type aromatase and its expression in male and female Wrasse, Pseudolabrus sieboldi

Pseudolabrus sieboldi, wrasse being a diurnal spawner provides a good opportunity to study the endocrine mechanism of estrogen formation in brain and gonads. Moreover, an extremely large amount of E2 was produced in serum and testis of wrasse. It is assumed that the presence of E2 may play a major role in diurnal gametogenesis in male fish. In this study brain type aromatase have been isolated, cloned and sequenced from the brain of wrasse. Further, the expression pattern of brain type aromatase in gonads and adult tissue of male and female fish have been analyzed. In addition, the diurnal expression pattern of brain type aromatase in both male and female fish brain during spawning season have been analyzed. The P450arom (br) was isolated, cloned and sequenced from both male and female bamboleaf wrasse. The P450arom (br) gene (1877 sequenced nucleotide) contains an ORF of 1470 bp, a 5′-UTR of 18 bp and at least 407 bp in 3′-UTR. The amino acid sequence homology in the coding region of wrasse P450arom (br) is high compared to that of medaka, Oryzias latipes (80%), rainbow trout type 2, Oncorhynchu mykiss (78.2%), fugu, Takifugu ribripes (78%) rainbow trout type 1, (76%), goldfish, Carassius auratus (66.8%) and zebrafish, Danio rerio (66.2%). Expression study reveals that P450arom (br) mRNA were most abundant in brains of both male and female fish throughout the day during the spawning season. RT-PCR study revealed that P450arom (br) was expressed in skin, anal fin and tail fin of both male and female wrasse. P450arom (br) was not detected at any time of the spawning day in either ovary or testis of wrasse.     

Home range and seasonal movement of taimen,Hucho taimen,in Mongolia

Gilroy DJ, Jensen OP, Allen BC, Chandra S, Ganzorig B, Hogan Z, Maxted JT, Vander Zanden MJ. Home range and seasonal movement of taimen, Hucho taimen, in Mongolia.
Ecology of Freshwater Fish 2010: 19: 545–554. © 2010 John Wiley & Sons A/S Abstract –  Taimen, Hucho taimen, is the world’s largest salmonid and a prized sport fish. We used radio and acoustic telemetry to characterise movements of adult taimen in an extensive river system, the Eg–Uur, in north‐central Mongolia. Forty‐six taimen were tagged with transmitters (27 radio, 17 acoustic and 2 radio‐acoustic combined) and tracked from 2004 to 2008 using mobile surveys and fixed receivers. The mean home range of individual taimen tracked for an average of 2.4 years was 23 km (N = 41, range = 0.5–93.2 km). Of the fish with over 10 relocations (N = 16), 90% remained within a range of 38 km. Four distinct movement patterns were observed: (i) restricted core home range, (ii) core range with seasonal departures, (iii) core range with separate seasonal range and (iv) home range transfer. Movement was greatest in May and June (spawning and postspawning period) with another peak period of movement in September and October (water temperature cooling).     

Effects of levamisole,echinacea, and thyme oral administration on growth factors,blood parameters,and immunity in beluga,Huso huso

A total of 180 fish (95.50 g) were fed with 0.1% levamisole, 0.5% thyme, and 0.5% echinacea crude extract for 60 days. The control group received no stimulant. The results of growth parameters indicated a significant positive effect of levamisole in comparison with the control group, though it was not significantly different from echinacea and thyme treatments (P > 0.05). No mortalities were observed in the treatments. Feed conversion ratio and protein efficiency ratio did not differ significantly between the treatments. The highest numbers of white and red blood cells and the greatest amounts of hemoglobin and hematocrit were detected in the levamisole treatment, showing significant differences with the other groups in the percentage of hemoglobin only (P < 0.05). The highest levels of MCH and MCHC were also measured in the levamisole treatment. Differential count of white blood cells revealed no significant differences in neutrophil and lymphocyte levels with the control. There was a significant increase in the percentage of eosinophil in the levamisole treatment alone. No significant differences were recorded in total protein, albumin, and aspartate aminotransferase and alanine aminotransferase enzymes. Levamisole treatment displayed the highest activities of glutathione peroxidase and lysozyme as well as the greatest amount of immunoglobulin. So 0.1 levamisole yielded better results in the growth, blood, and immunity parameters and can be used as a stimulant without injuring body organs.     

Age, growth, and reproduction of silky sharks, Carcharhinus falciformis, in northeastern Taiwan waters

To examine the age, growth and reproduction of silky sharks, Carcharhinus falciformis, in the waters off northeastern Taiwan, 469 specimens (213 females and 256 males) were collected from August 2000 to January 2002 at the Nanfanao fish market, northeastern Taiwan. The relationship between body weight (W) and total length (TL) for both sexes combined was expressed as: W = 2.92 × 10⁻⁶ TL^3.15 (n = 469, p < 0.01). The relationship between TL and vertebral centrum radius (R) for both sexes combined data was estimated as: TL = 25.979 + 18.197R (n = 250, p < 0.01). Growth bands (including translucent and opaque zones) in precaudal vertebrae formed once a year between December and January and were counted up to 11 and 14 for females and males, respectively. The von Bertalanffy growth function (VBGF) was used to model the observed length at age data. The sexes combined VBGF predicted an asymptotic length (L_∞) = 332.0 cm TL, growth coefficient (k) = 0.0838 year⁻¹, age at zero length (t₀) = −2.761 year (n = 250, p < 0.01). Size at 50% maturity for males was estimated to be 212.5 cm based on the logistic curve, which corresponded to 9.3 years. Females matured at 210–220 cm, which correspond to 9.2–10.2 years. The length at birth was estimated to be 63.5–75.5 cm TL. The number of embryos per litter was 8–10 and sex ratio of embryos was 1:1.     

Age,growth, maturity and the overfishing of the iconic sciaenid,Argyrosomus japonicus,in south-eastern,Australia

Mulloway (Argyrosomus japonicus) occur in estuarine and coastal waters surrounding Australia, Africa, India, Pakistan, China, Korea and Japan, where they are important in fisheries. This study identified that mulloway in south-eastern Australia had similar growth rates, but matured at smaller lengths and younger ages, to those in South Africa and Western Australia. Growth of both sexes was similar to about 5 years, after which females grew faster and attained a greater maximum length than males. Female mulloway matured at 4–5 years of age with a L₅₀ of 68 cm, whereas males matured at 2–3 years of age with a L₅₀ of 51 cm. The commercial fishery in New South Wales was characterised by declining catches and a reduction in the proportion of mulloway of mature lengths in landings. During 2002–2005 commercial landings were dominated (83%) by fish within 15 cm of the current minimum legal total length of 45 cm and aged 2 and 3 years (>80%), even though mulloway can attain lengths of 200 cm and live >30 years. Estimates of the rates of instantaneous total mortality ranged between 0.34 and 0.45, whilst the rate of instantaneous natural mortality (M) was estimated to be approximately 0.12. Yield-per-recruit analyses indicated that mulloway in New South Wales are being growth overfished and substantial increases in yield could be achieved by increasing the length at first harvest. Values of the spawning potential ratio were below 0.2 under a range of mortality estimates, suggesting that mulloway are at risk of recruitment overfishing. These results suggest that the spawning stock of mulloway in south-eastern Australia has been depleted and that remedial management action is required to protect this iconic species.     

上海地区池塘沉积物中氮、磷、有机碳及重金属风险评价

为综合评估上海地区池塘沉积物环境质量状况,2016-2019年对上海地区36个养殖场池塘采集沉积物样品360个,检测和分析沉积物中总氮（TN）、总磷（TP）、总有机碳（TOC）及重金属Cu、Zn、Pb、Cd、Cr、Hg和As含量。研究结果表明,池塘0~10 cm层和10~20 cm层沉积物中TN、TP、TOC及各重金属均值分布无显著差异（P>0.05）,0~20 cm层沉积物（干重）中TN、TP和TOC平均含量分别为（873.37±352.45）mg/kg、（685.66±199.66）mg/kg和（6.62±3.05）mg/g,三者相关性显著。综合污染指数法和有机指数法评价结果表明,池塘沉积物中氮和有机物质的累积量较低,磷累积量相对较高,均低于其他地区高产池塘。池塘沉积物中Cr、Cd、Cu和Hg样品超标率分别为6.42%、3.21%、4.13%和1.38%,Zn、Pb和As无超标现象。地质累积指数、潜在生态危害指数法和一致性沉积物质量基准评价结果表明,上海地区池塘沉积物中重金属整体上处于清洁等级、低潜在生态危害状态,预测不会引发生物毒性效应。     

Nitrogen,Phosphorus, and Carbon Budgets in Polyculture Ponds of Indian Major Carps and Giant Freshwater Prawn in Orissa State,India

A nutrient budget was quantified for six polyculture ponds of giant freshwater prawn (Macrobrachium rosenbergii) and Indian major carps (Catla catla, Labeo rohita, and Cirrhinus mrigala). The initial stocking size of prawn postlarva was 16.50 ± 0.54 mm with a body weight of 0.05 ± 0.001 g. Body weight of major carps was 9.0 ± 0.89 g. The duration of culture was 280 days. Feed accounted for 82% of total nitrogen (N), 93% of total phosphorus (P), and 95% of organic carbon (OC) inputs. Harvest of prawn and carps recovered 44% N, 1% P, and 19% OC. N, P, and OC accumulated in sediment were 47%, 73%, and 69%, respectively. Nutrient load in the harvest water was 0.67 ± 0.21 kg inorganic N, 0.15 ± 0.01 kg P, and 7.72 ± 0.62 kg OC per ton of Indian major carps and prawn.     

Growth, Condition, and Size Distribution of Paddlefish, Polyodon spathula, Juveniles Reared in Ponds at Three Densities

Abstract.— A study was conducted to determine the effect of increasing density on growth and size distribution of paddlefish, Polyodon spathula, juveniles reared in ponds. Feed‐trained paddlefish of mean weight (±SE) 25.8 ± 1.1 g were randomly stocked into nine 0.02‐ha ponds at 12,355, 18,533, and 24,710 paddlefish/ha, three replications per treatment. The fish were fed daily in excess of what they would eat for 97 d, beginning with a floating trout diet containing 45% protein and 16% lipid and then transferring to a floating catfish diet containing 32% protein and 4.5% lipid. Survival at harvest was not significantly different (P > 0.05) among treatments and averaged 90%. Mean final weights (±SD) for the low‐, middle‐, and high‐density treatments were 205.2 ± 54.1, 174.8 ± 53.2, and 178.6 ± 51.4 g, respectively. Best‐fit distributions centered on these means were lognormal. The low‐density distribution was significantly different (P < 0.05) from the two higher densities, which were not significantly different from each other (P > 0.05). Paddlefish weight at the minimum target length of 35 cm was estimated to be 100 g by regression analysis. The probability of paddlefish reaching or exceeding 100 g was 90% for the low‐density treatment. For the two higher densities, probabilities were 79 and 78%, respectively. Mean Fulton’s condition factors (FCFs) (±SD) were 250 ± 19, 242 ± 4, and 256 ± 37 for the low‐, middle‐, and high‐density treatments, respectively. The FCF for the middle‐density treatment was significantly lower than for the low‐ and high‐density treatments (P < 0.05), which were not significantly different from each other (P > 0.05). CV, feed conversion ratio, and relative growth were not significantly different (P > 0.05) among treatments and averaged 0.43, 1.50, and 5.45, respectively. Monoculture of paddlefish juveniles in ponds results in a hierarchic size structure when density is at least greater than 12,355 paddlefish/ha. The effect is enhanced with increasing density but becomes asymptotic as density approaches 18,533 paddlefish/ha. Feeding in excess does not ameliorate the effect.     

Isolation, sequence analysis, and characterization of androgen receptor in Southern catfish, Silurus meridionalis

Androgen receptor (AR), the mediator of androgen, plays important roles in the androgen signal pathway. In the present study, we isolated and analyzed the cDNA sequence and tissue distribution of androgen receptor in Southern catfish (scAR). The full-length cDNA of scAR contains 3,116 bp with an open reading frame (ORF) of 2,415 bp, encoding a protein of 804 amino acids (aa). Tissue distribution analysis of scAR revealed that it was expressed in all tissues examined, with no sexual dimorphism in the ovary and testis. Phylogenetic analysis and multiple amino acids sequence alignment indicated the close relationship and high similarity of scAR with ARs from cypriniform species. In addition, partial sequences of ARs from 7 other siluriform species were also isolated. Comparison of catfish ARs with those from other vertebrates revealed that an extra C-terminal tail of about 20aa exists in all the ARs from siluriform fishes investigated, but not in other ARs. The extra sequence was resulted from a 4-bp insertion before the stop codon of other vertebrate ARs, and it was identical in ARs from siluriform species of the same genus but different among ARs from species of different genera. We report here for the first time that the ARs from siluriform species are longer in C-terminal than those from other vertebrates and it might be useful in reconstruction of the phylogenetic relationship among siluriform fishes. The significance of the extra C-terminal tail for AR function remains elusive.     

Effect of Shelters,Densities, and Weight Groups on Survival,Growth and Limb Loss in the Freshwater Prawn,Macrobrachium nobilii (Henderson and Matthai, 1910)

Abstract

Three weight groups of juvenile prawn, M. nobilii, were raised at three stocking densities (22,38, and 77 prawns/m²) under three substrate conditions (sand, pebbles, and pebbles with shelter in the form of PVC pipe). Stocking density had a significant (P < 0.001) influence on the survival and growth of the prawn. A higher incidence of limb autotomy was recorded at the higher stocking density. Provision of pebbles and PVC pipe increased the survival, and mean growth rates, and reduced the percentage of limb loss.     

饵料、家系及二者交互作用对中间球海胆生长、摄食与变异的影响

为研究饵料、家系及二者交互作用对中间球海胆(Strongylocentrotus intermedius)生长、摄食及变异的影响,利用两种饵料—裙带菜(Undaria pinnitafida)和配合饲料,对6个中间球海胆全同胞家系进行了60 d投喂,实验结束时测量了实验海胆的终末体重(FBW)和总摄食量,并计算了体重特定生长率(SGR)、饵料系数(FCR)和体重变异系数的变化(ΔCV),利用双因素及单因素方差分析的方法,检验了饵料、家系及二者交互作用对FBW、SGR、FCR及ΔCV的影响。结果表明,饵料与家系的交互作用对FBW和ΔCV无显著影响,摄食裙带菜的海胆较摄食配合饲料的海胆在终末体重(FBW)和整齐度(ΔCV)上表现出极显著的优势(P0.01),不同家系间海胆的FBW和ΔCV也有显著差异(P0.05)。饵料与家系的交互作用对体重SGR和FCR均有极显著影响(P0.01),在各家系内,裙带菜投喂下海胆体重SGR均极显著优于配合饲料投喂(P0.01);饵料系数(FCR)均极显著高于配合饲料投喂(P0.01);对于每种饵料,不同家系间SGR也均具有显著差异(P0.05),FCR亦是如此。两种饵料下家系SGR排位基本一致,说明家系与饵料在SGR上的交互作用是由于不同饵料下家系对SGR的影响程度有差异造成的,而家系FCR排位出现了较大变化则说明FCR受到交互作用的影响是由于不同饵料对不同家系的投喂效果不同造成的。本研究结果表明,家系选择可用于对中间球海胆生长、饵料系数和整齐度的遗传改良,饵料对这些性状的表现有显著的影响,对饵料系数进行家系选育时应注意家系与饵料的交互作用问题。