Comparative study of catheter-mediated gene transfer into heart

ObjectiveTo investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI).MethodsFifteen dogs were used, and 03ml (1×10［9］ pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods.The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis.ResultsThe results showed that ① the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also foundin both vessels within the myocardium and liver; ② gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; ③ LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart,and also in the myocardium underlying the visceral pericardium.ConclusionThree catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose     

Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer

Objective To evaluate the effects of hepatocyte growth factor (HGF) on the prevention of scar formation and the promotion of wound healing by gene transfer. Methods A total of 12 female New Zealand rabbits were used in this study. Rabbits were anesthetized with an intravenous injection of sodium pentobarbital, and identical wounds were made over the ventral surface of each ear. Five circular wounds, 7 mm in diameter, were created in each ear by excision through the skin to the underlying cartilage using sterile technique. After the surgical procedures, 10 of the rabbits were randomly allocated to five groups, with 2 rabbits in each group: Ad-HGF group 1, Ad-HGF group 2, Ad-HGF group 3, Ad-GFP (a reporter gene) group and the solvent group. Immediately after surgery, 6×10(7) pfu Ad-HGF, 6×10(8) pfu Ad-HGF, 6×10(9) pfu of Ad-HGF, 6×10(9) pfu of Ad-GFP, or same volume of solvent (PBS, pH 7.2) was applied once to each wound in groups 1 to 5, respectively. One additional rabbit was used to evaluate the transfer efficiency of the adenovirus vector by transferring Ad-GFP (6×10(9) pfu) into its wounds. Ice slides of wounds from this animal were observed under fluorescence microscopy. Another additional rabbit was used to evaluate the expression of HGF and TGFβ1 after transferring Ad-HGF (6×10(9) pfu) into each of its wound. Immunohistochemistry was used for detection. Results The effect of HGF on reducing excessive dermal scarring was observed by adenovirus-mediated gene transfer. Transfection of the human HGF cDNA into skin wounds through an adenoviral vector suppressed the over-expression of TGFβ1, which plays an essential role in the progression of dermal fibrogenesis. Application of HGF to the wounds significantly enhanced wound healing and inhibited over scarring.Conclusion HGF gene therapy could be a new approach for preventing excessive dermal scarring in wound healing.     

血红素加氧酶-1基因转染对非协调性异种心脏移植物的影响

目的 观察血红素加氧酶-1(HO-1)基因转染对非协调性异种心脏移植物的影响,并探讨其可能的保护机制.方法 采用颈部袖套血管吻合法联合应用中华眼镜蛇毒因子(CVF)建立稳定的豚鼠-SD大鼠非协调性异种异位心脏移植模型.供体豚鼠和受体SD大鼠各24只,每组各8只随机配对分为Ⅰ组(对照组)、Ⅱ组(空白载体组)和Ⅲ组(HO-...     

腺病毒介导的脑源性神经营养因子表达对大鼠损伤脊髓轴突的保护作用

目的：研究腺病毒介导的脑源性神经营养因子（ＢＤＮＦ）基因在大鼠损伤疹髓内的表达规律,观察外源性ＢＤＮＦ对损伤脊髓的作用。方法：利用激光束描记位移的重力打击装置,制备可靠 的定量损伤模型;以直接法将带有ＢＤＮＦ基因表达框的复制制陷重组腺病载体直接转移至大鼠损伤脊髓,用Ｘ－Ｇａｌ染色检测基因转移有效性,并观察损伤轴突计量形态学改变,抽伤脊髓ＢＤＮＦｍＲＮＡ表达,ＢＤＮＦ和神经中丝（Ｎｅｕｒｏｆｉｌａｎ     

心包腔内注入腺病毒-血管内皮生长因子165基因治疗心肌缺血的实验研究

目的:探讨经心包腔途径转染血管新生基因对缺血心肌的血管生成及舒缩功能的影响。方法:第一部分:随机将12头中国小型猪分为实验组和对照组,每组6头。两组猪均采用球囊堵塞前降支第一对角支远端以建立心肌梗死模型,心肌梗死模型建立后即刻,采用经皮剑突下穿刺方法,将中心静脉导管插入心包腔内转染Ad-LacZ。以胶原酶1200 u及透明质酸酶3000u预处理心包后,在心包腔内注射含Ad-LacZ基因2.0×109pfu。对照组心包腔内注射生理盐水。分别于注射后3天、7天及28天处死动物,对缺血心肌进行染色及病理观察。第二部分:随机将20头中国小型猪分为实验组和对照组,每组10头,每组又分3天(n=2)、7天(n=6)及28天(n=6)三个亚组。注射后3天、7天及28天分别用免疫组化、超声心动图对缺血心肌血管新生情况进行检测,并以酶联免疫吸附试验(ELISA)检测血浆、心包及心肌组织中Ad-VEGF165的表达。第三部分:20头小型猪随机分为心包转染组(心包组)和冠脉转染组(冠脉组)。心包组和冠脉组均注射Ad-VEGF1651.0 ml(2×109pfu),于注射前及其后3、7、28天分别测定组织内VEGF水平、微血管密度(MVD)、心功能。结果:①实验组注射Ad-LacZ基因后第3天、第7天及28天后X-gal染色有阳性细胞,以第7天最明显,对照组无阳性细胞。②Ad-VEGF165基因经心包腔转染缺血心肌组织后,在心包及组织中成高表达,于7天达到高峰,28天降至基线水平,血浆中无目的基因的表达;28天时,实验组缺血心肌微血管密度(MVD)、心功能均明显高于对照组[MVD,517.0±75.7/mm2vs 226.5±54.1/mm2,P=0.009;LVEF72.11±5.2%vs 55.14±4.37%,P=0.005]。③心包组和冠脉组的心脏均表达有VEGF165基因,组织内VEGF水平在7天时达高峰,28天时降至基线水平,前组高于后组(702±85pg/ml vs 592±59 pg/ml,P=0.026)。而两组的MVD、心功能随转染时间延长均明显增加,但心包组优于冠脉组(28d,MVD,517.0±75.7/mm2vs 326.4±24.1/mm2,P=0.001;FS,32.9±2.2%vs 30.6±2.1%,P=0.049;LVEF,72.11±5.2%vs 65.87±2.16%,P=0.034)。结论:①应用球囊堵塞法可成功建立猪急性心肌梗死模型,胶原酶及透明质酸酶预处理心包后,腺病毒载体可转染缺血心肌,并持续表达4周。②用胶原酶及透明质酸酶预处理心包腔后,经其转染Ad-VEGF165可以诱导急性心肌梗死模型局部VEGF蛋白表达,促进缺血心肌组织血管新生并能改善心功能。③导管介导的心包腔与冠脉转染Ad-VEGF165基因治疗心肌缺血是有效的、切实可行的,而前者可能是更有前途的新方法。     

骨形态发生蛋白-4基因重组腺病毒载体的构建

目的:通过Cre/LoxP位点特异性重组系统构建携带人骨形态发生蛋白-4基因的重组腺病毒载体(Ad.BMP4)。方法:根据GenBank获得BMP4的基因序列合成引物,经PCR扩增获得长度为1230bp的BMP4基因片断,经酶切、测序鉴定后,与腺病毒穿梭质粒pSuCMV连接,随后与5'缺陷型腺病毒右臂的质粒pBGHloxPΔE1E3通过脂质体Lipofectamine2000共转染至293细胞,获得重组腺病毒Ad.BMP4,并予以扩增、纯化、鉴定。结果:经酶切电泳和测序证明获得的BMP4基因片断序列、大小和方向正确,目的基因插入的位置、方向正确,经重组、扩增和纯化后获得病毒滴度为2.9×109pfu/ml的重组腺病毒Ad.BMP4,以PCR扩增和测序的方法证实了所构建病毒含有目的基因的片断,安全性检测证明所构建的腺病毒无野生性腺病毒的存在。结论:通过Cre/LoxP位点特异性重组系统构建了腺病毒Ad.BMP4。采用的腺病毒载体点特异性重组的方法极大地提高了包装成功率,且包装成功的病毒颗粒均为含有目的基因的重组病毒,从而保证了重组过程的快速和高效。     

Inhibition of allergic responsiveness in a murine asthma model via IFN-γ transgene expression

Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lungs and the effect of mIFN-γ transgene expression on allergen-induced pulmonary eosinophil infiltration in a murine asthmatic model. Methods LacZ marker gene was transduced into CD-1 mouse airway epithelial cells by installation of a replication-deficient adenovirus with LacZ gene (AdCMVLacZ) 5×10[9]plaque forming unit (pfu) in the intratrachea or nostril.C57 mice were sensitized intraperitoneally and challenged by aerosol with ovalbumin (OVA) to produce an asthmatic model.AdCMVmIFNγ 5×10[9] pfu was administered via nostril in asthmatic mice 48 h before OVA challenge.Sera, bronchial alveolar lavage (BAL) and lungs were recovered 48 h after OVA challenge.Results After administration with AdCMVLacZ by intratracheal installation or nose-drop, the lungs revealed a high level of widespread LacZ transduction with X-gal staining, mainly along airways.IFN-γ via adenoviral vector transduction could be overexpressed both in vitro and in vivo (1624.7±1321.5 pg/ml in BAL 96 h after AdCMVIFNγ infection).In AdCMVIFNγ treated asthmatic models, histological evaluation revealed marked suppression of eosinophil peribronchial and perivascular infiltration; the recoverable percentage of eosinophils in BAL was an average of 9.00%±4.58%, which was a statistically significant decrease versus that of the positive control group (75.13%±6.85%) (P<0.001).The total cell number in BAL ((145±55.6)×10[3 cells/ml) in AdCMVmIFNγ treated mice also was tremendously reduced compared to the positive control group ((216.6±71.1)×10[3 ]cells/ml).Conclusions Adenoviral vector was able to overexpress exogenous gene in murine lungs.IFN-γ overexpression via adenoviral vector in pulmonary epithelia in vivo can abrogate allergen-induced eosinophilic infiltration in lungs in an asthmatic model, which may suggest a new preventively therapeutic method for cytokine immunogenetic transfer in allergic asthma.     

成年大鼠侧脑室注射与脑实质注射腺病毒表达载体转染脑细胞的比较研究

目的比较观察脑室注射和脑实质注射腺病毒表达载体转染脑细胞的范围及表达产物的程度。方法分别将Ad5CMV LacZ表达载体（20μl,病毒滴度为1．01×10^5pfu／ml）注入成年SD大鼠右侧脑室、尾壳核、内侧隔核-斜角带复合体和背侧海马,在注射后3～28d将大鼠脑制作成为切片,进行X-gal组织化学反应,观察被转染和表达的细胞形态和数目。结果在侧脑室内注射后3d,右侧侧脑室室管膜细胞及其下层开始有病毒载体转染和表达,继之向对侧侧脑室、第三脑室和第四脑室转染和表达产物,脊髓中央管壁偶尔有被转染和表达的细胞;而将等体积和等滴度的AdSCMV LacZ载体注入尾壳核、内侧隔核-斜角带复合体和背侧海马,则均出现直径约2～5mm的转染范围,表达产物的细胞离注射原位最远处约为5—6mm,细胞表达产物很局限并相对集中;而侧脑室注射病毒载体可以随脑脊液流动而使转染范围广,但被转染细胞主要集中在室管膜及其下层,离脑室壁最远处也只在0．5—2mm范围内。结论侧脑室注射病毒表达载体便于基因治疗弥漫性脑疾病;而脑实质注射病毒表达载体则利于基因治疗局部性脑疾病。     

Protective effect of adenovirus-mediated BDNF gene expression on spinal axon following in rats after spinal cord injury

The routine treatments of spinal cord injury are palliative in Practice at Present. Their goal is only to preventthe secondal injeq and the complications, and then thepatients to adapt new life style under palalysis condition.The sprouting and the Plasticity of the neims in foe centlal nervous system (CNS) Of malnlnals has been ProVedll]. It is wen known that the neurntrOPhins facilitatethe renovation and regenerahon of the ne~ns in CNS after injuries. HoweVer the c~ive effect Of the ne…     

Ad/CMV- hTGF-β1 Treats Rabbit Intervertebral Discs Degeneration in Vivo

To investigate therapeutic efficiency of Ad/CMV- hTGF-β1 gene for rabbit intervertebral disc degeneration model. 60 Japanese white rabbits were selected to form the L5-L6 Anterior-Lateral-Anulus-Fibrosus-Incision-Induced model in order to simulate human intervertebral disc degeneration. 36 rabbits, whose corresponding intervertebral discs were injected with 20 μl (10 × 106 pfu)of Ad/CMV- hTGF-β1 gene, constituted the therapy group, 12 were injected with 20 μl (10 × 106pfu)of Ad/CMV-LacZ gene as comparison group, while 12 were only injected with equivalent capacity of saline for empty comparison group. 3 weeks after injection, examples were taken for investigation of HE staining, MRI, Western Blotting and immunobistochemical research TGF-β1.Wide distribution of TGF-β1 was detected by immunohistochemical research in the degenerated annulus fibrosus after injection. Western Blotting research showed significant increase of TGF-β1 content in intervertebral discs treated with TGF-β1 gene than comparison groups. MRI signal transformed from low to comparatively high and that intervertebral disc pathological degree improved.Ad/CMV- hTGF-β1 gene transfection is a potential method to increase TGF-β1 content and reverse intervertebral disc degeneration.     

腺病毒携带的LacZ基因静脉注射后在大鼠体内的表达

目的 :探讨周围静脉注射的腺病毒载体介导的外源基因在体内不同组织的表达。方法 :将重组LacZ腺病毒经尾静脉注射导入Wistar大鼠体内 ,以X -gal染色法明确腺病毒载体介导的标识基因 (LacZ)在大鼠体内的表达部位和时间。结果 :β- gal表达具有剂量依赖性 ,而且存在器官、组织和细胞三种水平的表达差异。剂量较小时 ,肺、肾、肝、脾优先表达 ,而心肌在 1× 1 0 1 1 pfu/kg剂量组才有少量表达 ,大血管和脑组织始终未见表达。注射后 1～ 2周 ,大鼠的肾、肺、肝、脾、肾上腺高表达 β -半乳糖苷酶 ,3～ 4周表达量减少 ,5周基本消失。 结论 :腺病毒介导的外源基因经静脉途径转移 ,可能是肾、肺、肝的部分疾病基因治疗的有效基因转移途径 ,而对心脑血管疾病不适合。     

多药耐药基因RNAi重组腺病毒构建

目的 构建抑制多药耐药基因(MDR1)表达的RNAi腺病毒载体,探讨基因治疗改善癫痫多重耐药现象的可行性.方法 根据大鼠MDR1基因序列,选择3个19nt的靶序列,设计并合成3对66nt含编码短发夹RNA(shRNA)序列的寡核苷酸,构建pSIREN-shuttle-MDR1重组质粒,测序分析正确后转染通过马桑内酯诱导的已表达多重耐药蛋白的大鼠星形胶质细胞,通过RT-PCR法测定多药耐药蛋白(P-gp)表达量,判断所设计的3条DNA序列对于P-gp表达的抑制作用.选择抑制效率最高的1个重组质粒,将其中的MDR1 shRNA表达结构酶切后插入腺病毒载体pAdeno-X,构建的pAdeno-MDR1经Pac1酶切后与脂质体共转染HEK293细胞进行病毒包装扩增纯化,所得病毒液作酶切电泳及测序分析正确后再转染大鼠星形胶质细胞模型.RT-PCR及免疫组织化学法分别测转染前后星形胶质细胞模型的MDR1及P-gp表达量.结果 重组质粒及pAdeno-MDR1病毒经PCR、酶切、测序分析证实构建正确.病毒滴度为6×109 pfu/mL.重组腺病毒转染星形胶质细胞后MDR1及P-gp表达量减少,干扰效率接近100%.结论 成功构建针对大鼠MDR1基因的RNAi腺病毒载体,并通过体外实验证实其对大鼠MDR1基因的高效抑制作用.为进一步探索难治性癫痫的多药耐药机制和基因治疗奠定了基础.     

Angiogenesis effects of adenovirus—mediated gene transfer of VEGF—B on chronic ischemic myocardium

Objective: To study the angiogenesis effects of adenovirus-mediated gene transfer of VEGF-B on chronic ischemic myocardium. Methods: Domestic pigs underwent thoracotomy and placement of an ameroid constrictor on the circumflex coronary artery. Four weeks later, Ad. VEGF-B, Ad. LacZ or PBS were administrated directly into the myocardium at 10 sites in the circumflex distribution (109 PFU or 100 μl) according to groups. Echocardiography and ex vivo coronary angiography were performed. The injection sites around myocardium were harvested and subjected to histological analysis and immunochemical staining. Results: E-chocardiography assessment 4 weeks after vector administration demonstrated significant improvement of regional wall systolic function. Collateral vessel development assessed by angiography was also significantly greater in Ad. VEGF-B animals than that in control animals. Vascular density analysis revealed a mean of 43±5 neovessels per high-power field in Ad. VEGF-B group versus 19±4 and 17±6 in     

hNET基因重组腺病毒载体构建和体外表达

目的以复制缺陷的腺病毒为载体,用细菌内同源重组法构建含有人的去甲肾上腺素转运子(human neurotransmitter transporter,hNET)基因的腺病毒载体。方法利用限制性内切酶KpnⅠ XbaⅠ从pCMV5质粒中切下目的基因hNET,亚克隆至经同样酶切的腺病毒穿梭质粒pAdtrack-CMV,形成重组穿梭质粒pAdtrack-CMV-hNET,PmeⅠ酶切线性化后,与pAdeasy-1在大肠杆菌BJ5183中进行同源重组,获得含目的基因的重组体质粒Ad-hNET,PacⅠ酶切后用脂质体Lipofectamine2000转染HEK293细胞,包装成重组体腺病毒。采用PCR技术和Western Blotting对重组体腺病毒进行鉴定,利用穿梭质粒pAdTrack-CMV中带有绿色荧光蛋白报告基因检测表达,经过扩增和纯化,测定病毒滴度。结果测序结果证明hNET序列的正确,PCR、PacⅠ酶切电泳和Western Blotting证实腺病毒重组质粒Ad-hNET构建成功。扩增纯化后测定重组病毒Ad-hNET的滴度为1.2×1010pfu/mL。结论成功构建了能表达hNET基因的重组腺病毒载体,为肿瘤的靶向治疗提供前期的研究工作。     

大鼠ZnT1基因重组腺病毒载体的构建及鉴定

目的利用GatewayTM技术构建含大鼠锌转运体1(ZnT1)基因重组腺病毒载体,鉴定外源基因在真核细胞中的良好表达。方法采用化学合成的方法,合成ZnT1/RES/EGFP目的基因片段,通过PCR方法在基因片段两端加入attB重组位点,与含有attP重组位点的pDonr221供体载体BP反应形成入门载体pDown-ZnT1。将含有attL重组位点的入门载体与含有attR位点目的载体Ad/CMV/V5-DEST通过LR反应形成腺病毒表达载体pAd-ZnT1。酶切和测序鉴定后,由PacⅠ酶切线性化转染HEK293A细胞包装,提取病毒颗粒。采用终点稀释法测定重组腺病毒滴度;Western blotting技术分析目的蛋白表达情况。结果目的基因按正确的方向重组入克隆载体中,重组腺病毒表达载体在HEK293A细胞中包装成功,获得成熟的腺病毒颗粒,病毒滴度为1.6×108 pfu/L,在HEK293A细胞中高表达。结论该实验采用GatewayTM技术构建了含有大鼠ZnT1基因的重组腺病毒载体,为下一步研究该基因在脊髓神经细胞中的表达以及与BDNF/TrkB信号调节通路的关系奠定了基础。     

小鼠BTLA基因重组慢病毒载体的构建及鉴定

目的 :构建小鼠B、T淋巴细胞衰减子(BTLA)基因重组慢病毒载体,探讨BTLA基因过表达对小鼠脾T淋巴细胞增殖及活化的影响。方法:以小鼠脾脏组织总RNA为模板,逆转录为c DNA,通过PCR技术扩增BTLA基因,构建p WPTS-m BTLA慢病毒载体,磷酸钙法感染人胚肾上皮细胞株293T细胞。RT-PCR及Western blot法检测BTLA m RNA和BTLA蛋白表达,50%组织培养感梁剂量(TCID50)法检测重组慢病毒滴度。通过感染p WPTS-m BTLA及p WPTS-GFP慢病毒载体的293T细胞与小鼠脾脏T淋巴细胞混合培养,初步研究BTLA基因过表达对小鼠脾T淋巴细胞活化与增殖的影响。结果:成功构建小鼠p WPTS-m BTLA慢病毒载体,并制备高滴度病毒颗粒(1.3×108pfu/ml)。通过对比实验组与对照组小鼠脾T淋巴细胞的增殖结果 ,发现4 d及8 d T细胞的增殖效应均明显受抑制,差异具有统计学意义(P<0.05),且这种抑制作用在0~8 d内具有时间依赖性。结论:过表达BTLA基因的293T细胞与小鼠脾T淋巴细胞混合培养后对其增殖及活化有抑制作用,提示成功构建具有生物学效应的小鼠BTLA基因重组慢病毒载体。     

重组胶质细胞源性神经营养因子腺病毒保护小鼠中脑多巴胺能神经元

目的：观察携带大鼠来源的胶质细胞源性神经营养因子(rGDNF)的重组腺病毒在小鼠帕金森模型中有无保护中脑多巴胺(DA)能神经元对抗神经毒素甲基-苯基-四氢吡啶(MPTP)损伤的作用。方法：将LacZ重组腺病毒(Ad-LacZ)通过脑立体定位仪注入小鼠单侧纹状体，分别于注射后24h、72h、10d和30d灌流取脑做X-Gal染色，观察报告基因在小鼠脑内的表达情况；将rGDNF重组腺病毒(Ad-rGDNF)通过脑立体定位仪注入小鼠单侧纹状体。两者均于72h后给予MPTP损伤中脑DA能神经元，1周后用高压液相色谱分析纹状体中DA、3，4-二羟基苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果：Ad-LacZ注射后24h表达较弱，72h逐渐增强，10d仍可见到较强表达，30d则逐渐减弱。LacZ基因的表达范围比较局限，胶质细胞和神经元都能被感染，并且未见明显的腺病毒载体引起的细胞毒性作用。注射Ad-rGDNF侧纹状体DA含量较MPTP损伤组和MPTP Ad-LacZ组显著升高，而未注射侧无明显升高。此外，DA主要代谢产物DOPAC和HVA的含量也明显升高，而DOPAC／DA、HVA／DA比值则明显降低。结论：Ad-rGDNF能显著保护中脑DA能神经元对抗MPTP的损伤。     

人凝血酶敏感蛋白1抗血管生成活性片段重组腺病毒高效制备

目的构建人凝血酶敏感蛋白1(thrombospondin1,TSP1)抗血管生成片段重组腺病毒。方法采用RT-PCR方法从正常人外周血单个核细胞扩增编码TSP1抗血管生成活性片段(TSP1f)的cDNA序列,并将其克隆至穿梭质粒pShuttle-CMV上,以线性化重组穿梭载体与超螺旋腺病毒骨架质粒pAdEasy-1共转化大肠杆菌BJ5183,进行同源重组并酶切鉴定筛选正确重组子,用线性化重组质粒转染人胚肾293细胞,制备重组腺病毒ADV-TSP1f,最后以PCR及Westernblot方法鉴定TSP1抗血管生成片段的表达。结果细菌内同源重组共获得43个卡那霉素抗性克隆,经酶切鉴定表明其中直径最小的10个均为正确重组子,该重组质粒转染293细胞所获得的重组腺病毒可高效表达TSP1抗血管生成片段,纯化后病毒滴度为1.0×1011pfu/mL。结论细菌内同源重组法构建重组腺病毒高效快捷,所获得的ADV-TSP1f可进一步应用于抗肿瘤血管生成的基因治疗研究。     

Effect of local viral transfer of interleukin 10 gene on a rabbit arthritis model induced by interleukin 1β

Backgroud Interleukin 16 （IL-1β） is the principal mediator in the pathogenesis of rheumatoid arthritis. Continuous injection of interleukin 1β （IL-1β） into the knee articular cavities of anamals can induce models that resemble rheumatoid arthritis. The objective of this study was to evaluate the feasibility of local recombinant retrovirus viral interleukin 10 （rRV-vlL-10） gene transfer treatment of a rabbit model of arthritis induced by IL-16. Methods An hlL-1β-induced rabbit rheumatoid arthritis model was established using the MFG-hlL-1β-neo-HIG-82 cell line, which is capable of continuous secretion of hlL-1β. After transfecting the rabbit synovial fibroblast cell line （MFG-hlL-1β-neo-HIG-82） with rRV-vlL-10, G418 was then added to identify the positive clone. The rRV-vlL-10 positive clone was injected into the established rabbit rheumatoid arthritis model through intra-articular injection. Successful gene transfer was determined by reverse transcripUon-polymerase chain reaction （RT-PCR） and immunohistochemistry. The levels of IL-1β before and after treatment were determined by enzyme- linked immunosorbent assay. Results Retrovirus vector was an effective vector both to synoviocytes in vitro and synovium tissue in vivo as confirmed by RT-PCR and immunohistochemistry. The rabbit arthritis model treated with rRV-vlL-10 showed a dramatic remission of arthritis and a decline in the level of cytokines such as IL-1β. Conclusions Retrovirus-mediated transfecUon of vlL-10 successfully transferred the gene into rabbit synovium ex vivo and was able to suppress intra-articular inflammation response to IL-1β.