RP-HPLC测定上感口服液中丹皮酚含量

应用RP -HPLC测定上感口服液中丹皮酚的含量。采用ODS色普柱 ( 2 5 0mm× 4 6mm ,5 μm) ,甲醇-水 -冰醋酸 ( 65∶35∶0 5 )为流动相 ,检测波长为 2 74nm。丹皮酚在 2 5～ 30 μg ml-1浓度范围内呈良好线性关系 (r =0 9999) ,平均回收率为 95 7%,RSD =2 1%(n =6)。方法准确、灵敏 ,回收率高。     

高效液相色谱法测定风湿定片中丹皮酚的含量

目的:对风湿定片中的丹皮酚进行含量测定.方法:采用HPLC法,以Diamonsil-C18为色谱柱,以甲醇-水(50:50)为流动相,流速为1.0 mL/min,检测波长为274姗.结果:丹皮酚在6～70 μg/mL范围内线性关系良好,相关系数r=0.9999;平均回收率为99.60%,RSD=1.23%(n=6).结论:本方法结果准确,重现性好,可用于风湿定片中丹皮酚的含量测定.     

利心丸中丹皮酚的含量测定方法研究

目的 建立利心丸的含量测定方法。方法 采用高效液相色谱法测定利心丸中丹皮酚的含量。采用Diamonsil C18(4.6×250 mm,5 μm)色谱柱,以甲醇-水作为流动相,流速0.8 mL.min-1,柱温35 ℃,检验波长为274 nm。结果 丹皮酚在0.020～0.503 μg范围内,线性关系良好(r=1.0000);平均回收率为102.4%,RSD为1.1%。结论 该方法操作简单、专属性强、重复性好,可作为利心丸中牡丹皮的质量控制。     

RP-HPLC法测定骨刺消痛胶囊中丹皮酚的含量

目的:建立以反相高效液相色谱法测定骨刺消痛胶囊中丹皮酚含量的方法。方法:色谱柱为Shim-pack C18(250mm×4.6mm,5μm),流动相为甲醇-水(50∶50),流速为1.0mL·min-1,检测波长为274nm。结果:丹皮酚进样量在0.452～2.260μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为97.26%,RSD=1.62%(n=9)。结论:本方法操作简便、快速、准确,可作为丹皮酚的定量分析方法。     

HPLC测定茂丹皮中的丹皮酚

目的 采用RP-HPLC测定茂丹皮中的丹皮酚.方法 以水蒸气蒸馏法处理样品;选用C18色谱柱,流动相为甲醇-水(55:45),流速0.9 mL·min-1,检测波长274nm,柱温35℃.结果 进样量0.0221～2.2120μg与峰面积线性关系良好(r=0.9999);回收率平均为98.15%,RSD=2.03%(n=5).结论 所用方法准确、重复性好,可定量分析茂丹皮中的丹皮酚.     

HPLC同时测定桂枝茯苓丸中的肉桂酸和丹皮酚

目的 建立桂枝茯苓丸中肉桂酸和丹皮酚的测定方法.方法 采用HPLC法,色谱柱为Phenomenex C18(250 mm × 4.6mm,5 μm),流动相为乙腈-水-冰醋酸(30:70:0.5),流速为1.0 ml·min-1,柱温为25℃,检测波长为274 nm.结果 肉桂酸和丹皮酚的线性范围分别为5.105～51.050 ng(r=0.9998)和97～970 ng(r=0.9998),平均回收率分别为97.6%(RSD=1.54%)和96.9%(RSD=1.73%).结论 所建方法准确、简便、快速,适用于桂枝茯苓丸的质量控制.     

高效液相色谱法测定珍珠胃安丸中丹皮酚含量

目的建立测定珍珠胃安丸中丹皮酚含量的高效液相色谱法。方法采用Apollo C18-A色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(50∶50)为流动相,检测波长274 nm,流速1.0 mL/min。结果丹皮酚进样量在0.050 7～1.014μg范围内与峰面积线性关系良好,r=0.999 8(n=6);平均回收率为98.32%,RSD=1.14%(n=6)。结论高效液相色谱法简便、灵敏、稳定、准确,可作为珍珠胃安丸中丹皮酚含量的测定方法。     

HPLC法测定复方姜黄降脂片中丹皮酚的含量

目的:建立复方姜黄降脂片中丹皮酚的含量测定方法.方法:采用迪马C18色谱柱(200nm×4.6mm,5μm),以甲醇一水(72:28)为流动相,检测波长:274nm.结果:丹皮酚的含量测定线性范围为0.05034 μg～0.5034 μg(r=0.9999,n=6),平均回收率为98.25%,RSD=0.66%(n=5).结论:所建立的方法简便、准确可靠,重现性好,可作为复方姜黄降脂片中的丹皮酚的定量分析方法.     

HPLC测定养阴清肺糖浆中丹皮酚的含量

目的 建立HPLC测定养阴清肺糖浆中丹皮酚的含量.方法 用Dikma-C18色谱柱,以甲醇-水(70:30)为流动相,检测波长为274 nm.结果 养阴清肺糖浆中丹皮酚的线性范围:4～8 μg·ml-1(r=0.999);加样平均回收率为99.61%,RSD=0.76%.结论 本法专属性强,简便,准确,稳定,可用于养阴清肺糖浆的质量控制.     

高效液相色谱法测定云香精中丹皮酚含量

目的 建立测定云香精中丹皮酚含量的高效液相色谱法.方法 采用Agilent TC-C18柱(150mm×4.6mm,5 μm),流动相为乙腈-水-三乙胺-磷酸(28:72:0.1:0.1),检测波长为274 nm.结果 丹皮酚进样量线性范围为0.10～1.20μg,平均加样回收率为99.89%,RSD为1.27%(n=6).结论 该方法简便可行、结果可靠,可控制云香精的质量.     

丹皮酚的药理作用、提取及含量测定方法研究进展

牡丹皮是中国传统大宗中药材,具有很高的药用价值,其主要药用成分丹皮酚近年来也受到广泛关注。该文就近年来有关丹皮酚的理化性质、药理作用、检测提取等方面的研究成果进行了总结,并对其未来研究方向进行了展望。     

丹皮酚的研究进展

牡丹皮是一种传统中药,丹皮酚是其主要药效成分。临床上丹皮酚主要外用抗过敏,但近年来研究发现丹皮酚对多种疾病有良好的治疗作用。本文主要综述近年来研究发现的在心血管系统、肝纤维化、抗肿瘤治疗等方面丹皮酚的治疗作用。     

Diuretic action of paeonol.

Effects of paeonol, a major component of the root bark of PAEONIA SUFFRUTICOSA, on urinary excretion of water (UV), sodium (U (Na)V), potassium (U (K)V), chloride (U (cl)V) and osmolality (U (osm)V) were investigated in bicarbonate saline loaded rats. Paeonol at the doses of 62.5 to 250 mg/kg p.o. produced dose-dependent increases in UV, U (Na)V and U (Cl)V (P < 0.05 or 0.01 vs. control). U (K)V was unchanged at the lower doses and decreased at the highest dose. U (osm)V was also increased by paeonol (P < 0.05 or 0.01). The lowest effective dose of paeonol in diuretic action was 62.5 mg/kg which is somewhat lower than those reported to produce an antiinflammatory action or a reduction of gastric secretion (125 to 500 mg/kg). The highest dose of paeonol produced the same degree of increase in UV as hydrochlorothiazide (HTZ) (10 mg/kg), while the increases in electrolytes excretion by HTZ were significantly greater than by paeonol. These data demonstrate that paeonol has a diuretic action, and suggest that its sites of action in the kidney are different from those of HTZ.     

丹皮酚脂质体凝胶的质量控制研究

目的 建立丹皮酚脂质体凝胶的质量控制方法.方法 采用薄层色谱(TLC)法对凝胶中丹皮酚进行定性鉴别;采用高效液相色谱法测定凝胶中丹皮酚.结果 TLC斑点清晰,专属性强,阴性无干扰;丹皮酚质量浓度在8.32-49.92μg/mL与峰面积积分值呈良好的线性关系,平均回收率为99.14%,RSD为0.86%.结论 该定性鉴别...     

Study on the pharmacokinetics and metabolism of paeonol in rats treated with pure paeonol and an herbal preparation containing paeonol by using HPLC-DAD-MS method

Paeonol, a principal bioactive component of the Chinese herb Moutan Cortex with anti-inflammatory and analgesic effects, was comparatively studied to determine its pharmacokinetic behavior and metabolic profile in rat following oral administration of the pure paeonol alone and an herbal preparation "Qingfu Guanjieshu" (QFGJS) containing paeonol. An HPLC-DAD method was developed and validated for determining the concentration of paeonol in rat plasma. The in vivo time curves and AUC of paeonol at three doses were increased in a dose-dependent manner, while the pharmacokinetic parameters of paeonol in QFGJS at a comparable dosage were significantly elevated in comparison with those of pure paeonol. By using LC-Q/TOF-MS technique, four metabolites of paeonol were identified in plasma at 5min after dosing, with T(max) around 20min after treatment with the pure paeonol or QFGJS. Interestingly, relative concentrations of metabolites P2, P3 and P5 were markedly increased in plasma of rats treated with QFGJS compared with those of pure paeonol. These results indicate that other components in QFGJS could effectively influence the pharmacokinetic behavior and metabolic profile of paeonol in rats. The current studies emphasize the significance of the research toward an understanding of pharmacokinetic interactions of the co-existing components in the herbal preparations.     

Anxiolytic-like effect of paeonol in mice

The present study in mice compared the putative anxiolytic-like effect of paeonol, a phenolic component from the root bark of Paeonia moutan, with the benzodiazepine diazepam in the elevated plus maze and the light/dark box-test. The comparison was also with regard to locomotor activity (open-field test) and myorelaxant potential (inclined plane test). As with 2 mg/kg diazepam, paeonol (at 17.5 mg/kg) increased the percentage of time spent on open arms in the elevated plus maze and increased the time spent in the light area of the light/dark box (at 8.75 and 17.5 mg/kg). Since paeonol, in contrast to diazepam, had no effect on either the number of squares entered in the open-field test or in the inclined plane test, its side-effect profile is considered as superior to the benzodiazepine.     

丹皮酚前体脂质体的研制及其特性

目的：研究丹皮酚前体脂质体的制备方法，提高丹皮酚脂质体的稳定性。方法：采用薄膜一超声一冷冻干燥法。选择合适的冻干保护剂制备丹皮酚前体脂质体，并对水合后脂质体的形态、粒径、包封率和稳定性进行考察。结果：选择10％的蔗糖为保护剂。制得的丹皮酚前体脂质体经水合后主要为单室脂质体，粒径分布较均匀。平均粒径149．7nm．药物包封率为73．9％，25℃贮存6个月，外观、含量及包封率无明显变化。结论：该方法制备丹皮酚前体脂质体可行，包封率较高且有良好的稳定性。     

丹皮酚抗肿瘤作用机制的研究进展

丹皮酚是牡丹皮的主要活性成分,其抗肿瘤作用的研究已经受到广泛关注。研究报道了丹皮酚对肝癌、胃癌、乳腺癌、卵巢癌等多种肿瘤均具有抗癌作用。丹皮酚主要通过抑制肿瘤细胞增殖,影响肿瘤细胞周期,调控凋亡相关因子及相关通路,抑制肿瘤细胞转移,逆转化疗药物耐药性及放疗增敏发挥抗肿瘤作用。主要综述了丹皮酚抗肿瘤作用机制的最新研究进展。目前丹皮酚抗肿瘤的研究仍处在初步阶段,还需进一步加强对信号转导通路、调控靶点、抑癌基因、联合用药及体内实验等方面的研究。     

HPLC法测定复方丹皮汤中丹皮酚的含量

目的建立用HPLC法测定复方丹皮汤中丹皮酚含量的方法。方法以十八烷基硅烷键合硅胶为填充剂;甲醇-水（50：50）为流动相;检测波长为274nm。结果丹皮酚含量在5．36～26．8mg·L-1浓度范围线性关系良好,r=0．9997,平均回收率为98．71％,RSD=1．95％（n=6）。结论该方法方便快捷,准确,灵敏度高,重现性好。     

六味地黄丸中丹皮酚的电化学检测

目的建立六味地黄丸中丹皮酚的电化学检测方法。方法循环伏安(CV)、差分脉冲(DPV)及紫外和高效液相色谱等。结果在0.1 mol.L-1的NaOH-NaH2PO4(pH=11)底液中、100 mv.s-1的扫描速度下丹皮酚在铂电极上有一灵敏的氧化峰,峰电流与其浓度在5.0×10-6~1×10-3mol.L-1的范围内呈良好的线性关系,线性回归方程为:I=0.23-4082.64×C(r=0.998 4),检出限为1×10-6mol.L-1。应用于六味地黄丸中丹皮酚的检测,结果令人满意。结论该方法准确可靠,操作简便、快速,重现性好,无干扰。