3种银汞黏接剂抗微渗漏的效果研究

目的检验三种银汞黏接剂应用于粘接银汞修复时,其抗微渗漏的能力,寻找一种简单有效的黏接材料。方法选28颗新鲜前磨牙,在其近中及远中邻面制备边长分别是2 mm和4 mm的长方形,使其龈壁位于牙本质-牙骨质界,牙合壁位于牙釉质。28颗前磨牙按黏接材料的不同分为4组:空白对照组,树脂加强玻璃离子水门汀组,玻璃离子水门汀组,树脂型银汞黏接剂组。充填后,37℃水浴7 d,然后浸入亚甲蓝染色液中,37℃恒温染色48 h,统计其微渗漏结果。结果非参数统计显示实验组微渗漏小(P<0.01),树脂加强型玻璃离子组无微渗漏,与其他组有着显著差异(P<0.05),釉质侧的渗漏低于牙本质-牙骨质侧。结论粘接银汞修复是一项有效的技术,并且用树脂加强型玻璃离子作黏接剂有着更有效的防微渗漏效果,值得在临床推广应用。     

Microleakage in conventional and bonded amalgam restorations: influence of cavity volume

This study verified the relationship between the volume and microleakage of conventional and bonded amalgam restorations. Also, the microleakage influence of intermediate materials, substrates and the direction of sectioning was investigated. Fifty-six bovine incisors were selected. Standard Class V cavities were prepared in buccal and lingual surfaces. For each tooth, two cavity sizes were prepared, corresponding to two cavity volumes: one larger (A) and the other smaller (B). The cervical wall was located in cementum/dentin and the incisal wall in enamel. The teeth were distributed in four groups (n=28) according to the intermediate material employed (glass-ionomer cement, resin cement, adhesive system and copal varnish-control). The materials were applied following manufacturers' directions. After restoration, the teeth were submitted to thermal cycling. They were then immersed in a dye solution and sectioned in two directions inciso-cervical (IC) and mesio-distal (MD) sections to evaluate the microleakage. Data were subjected to non-parametric statistical analysis (Wilcoxon's paired test and Kruskal-Wallis test). No significant difference was found between the two cavity sizes. Leakage in enamel was statistically lower than in the cementum/dentin interface (p < 0.05). In some situations, glass-ionomer or resin cement lined amalgam restorations presented less dye leakage than copal varnish lined restorations (p < 0.05). No significant difference was observed in microleakage between IC or MD sectioning. Within the limitations of this study, it was concluded that cavity size and direction of section were not significant factors for microleakage, while substrate and intermediate materials had a significant effect on the sealing ability in amalgam restorations.     

Comparison of microleakage of three acid-base luting cements versus one resin-bonded cement for Class V direct composite inlays

STATEMENT OF PROBLEM: Demineralized dentin beneath set cement may adversely affect microleakage under fixed restorations. PURPOSE: Microleakage of direct composite inlays cemented with acid-base cements and a methyl methacrylate resin cement were evaluated to determine their effect on the integrity of the underlying hybridized dentin. MATERIALS AND METHODS: Sixty Class V box preparations (3 mm x 3 mm x 1.5 mm) were precisely prepared in previously frozen bovine teeth with one margin in enamel and another margin in dentin. Direct composite inlays (EPIC-TMPT) for each preparation were divided into 4 groups of 15 specimens each and cemented with 3 acid-base cements (control group): Elite, Ketac-Cem, Hy-Bond Carbo-Cem, and 1 adhesive resin cement: C&B Metabond. All specimens were stored in distilled water for 24 hours at 37 degrees C before immersion in 0.5% basic fuchsin for 24 hours. The dye penetration was measured on the sectioned specimens at the tooth-cement interface of enamel and cementum margins and recorded with graded criteria under light microscopy (Olympus Vanox-T) at original magnification x 50, 100, and 200. A Kruskal-Wallis and the Mann-Whitney test at P<.05 were used to analyze leakage score. RESULTS: All cementum margins of the 3 acid-base cements tested demonstrated significantly higher leakage scores than cementum margins for inlays cemented with the resin cement tested(P<.01). No leakage along the tooth-cement interface was found for inlays retained with the adhesive resin cement. CONCLUSIONS: Within the limitations of this study, the 3 acid-base cements tested exhibited greater microleakage at the cementum margins than did the adhesive resin cement that was tested.     

Influence of flowable liner and margin location on microleakage of conventional and packable class II resin composites

This in vitro study evaluated gingival wall microleakage in packable and microhybrid conventional composite restorations with and without a flowable composite liner. Each group was evaluated with gingival margins situated in both enamel and cementum/dentin. Two hundred and forty Class II cavities were prepared in extracted third molars, half with gingival margins in enamel and half with margins in dentin/cementum. In groups of 30, restoration was undertaken with packable alone (3M Filtek P60), conventional alone (3M Z250), packable plus flowable liner (3M Filtek Flow) and conventional plus flowable liner. All used 37% phosphoric acid etch and Scotchbond 1 (3M) as the bonding system. After restoration, the teeth were thermocycled (between 5 degrees C, 37 degrees C and 60 degrees C) 1,500 times, soaked in 0.1% methylene blue, sectioned and microleakage from the gingival margin scored. Statistical analysis was performed using Kruskal Wallis and Mann-Whitney U tests. There was no significant difference between systems in terms of leakage scores when gingival margins were situated in enamel (p=0.70). All restorations with margins in cementum/dentin leaked significantly more than those with margins in enamel (p<0.001). There was no significant difference between leakage scores of 3M Z250 and Filtek P60 with cementum/dentin gingival margins (p=0.68). Use of a flowable composite liner (3M Filtek Flow) against cementum/dentin was associated with increased microleakage (p<0.001). In this study, leakage scores suggest that gingival margins should be placed in enamel. The conventional and packable resin composites tested were not associated with differences in microleakage. Leakage data do not support the use of flowable resin composite linings in Class II resin composite restorations.     

Microleakage and SEM interfacial micromorphology of amalgam restorations using three adhesive systems

OBJECTIVE: This study evaluated the microleakage and interfacial micromorphology of Class V cervical amalgam restorations lined with OptiBond, AElitebond, or Panavia 21. METHODS: Unlined amalgams served as control. Cavities were treated with each dentin bonding system according to the manufacturers' instructions and restored with Tytin non-gamma 2 spherical amalgam. After one week of storage in tap water at 37 degrees C, the specimens were thermocycled (1000 cycles, 6-60 degrees C, 30s dwell time). Microleakage was assessed by means of basic fuchsin dye penetration and recorded according to an ordinal scale. RESULTS: None of the systems tested in this study completely eliminated microleakage. Kruskal-Wallis one-way ANOVA and Mann-Whitney U test found that on the occlusal wall, Panavia 21 and the control group had the least leakage (P<0.05). No statistically significant differences were found at dentin margins (P>0.05). Wilcoxon matched pairs signed rank test found that Panavia 21 and the control group had less leakage at the occlusal than at the dentin margins (P<0.05); when AElitebond and OptiBond groups were evaluated, microleakage at the enamel and at the dentin margins was similar for each group. With the adhesive systems, perhaps the hydrophilic bonding agents incorporated the dye during specimen immersion and/or sectioning. CONCLUSIONS: The use of adhesives may not be as worthy as resin cements for sealing and bonding amalgam restorations to enamel and dentin.     

Microleakage of all-ceramic crowns using self-etching resin luting agents

Self-etching adhesive systems are a new generation of materials that possess acidic methacrylates that can generate self-adhesion. There is limited data reported on the marginal leakage of ceramic restorations bonded with self-etching adhesive materials. This study assessed and compared the amount of microleakage of bonded ceramic crowns using three different types of self-etching adhesive systems with and without a die spacer. Eighteen human molars were prepared for all-ceramic IPS Empress crowns and the teeth were randomly assigned to each experimental group. The buccal side had the preparation finish line 1.5 mm below the CEJ, and the lingual finish line was 1.5 mm above the CEJ, creating margins in enamel and dentin. Two die-spacing techniques were used (three layers or no layer of die spacer). Each crown restoration was cemented with one of three self-etching resin luting agents (Panavia F 2.0, Multilink and RelyX Unicem). The specimens were thermally cycled for 1000 cycles, then immersed in a 5% methylene blue dye solution for 24 hours. The teeth were then rinsed, embedded in clear epoxy resin and sectioned. A total of 60 sections were evaluated for each type of resin luting agent using digital image analysis at 70x magnification. A novel formula, using mean percentage of microleakage, was developed by dividing the extent of dye penetration along the tooth/resin luting cement interphase and the total perimeter of the tooth crown surface. The data were analyzed using three-way analysis of variance at the 0.05 level of significance. Fisher's PLSD intervals were calculated for comparing significant means. Panavia F 2.0 showed a lower degree of microleakage than RelyX Unicem and Multilink at both the enamel and dentin margins. Interactions of the main effects (cement, margin and die spacer technique) were all highly significant (p< or =0.004). The degree of microleakage was higher on the dentin margins than on the enamel margins (p<0.0001). The degree of microleakage for the die spacer group was not significantly different from the group with no die spacer technique (p>0.1). Overall, Panavia F 2.0 showed the least microleakage, followed by RelyX Unicem and Multilink, respectively.     

Microleakage of Class V restorations using two different compomer systems: an in vitro study

This study compared the marginal microleakage of Class V cavities restored with Dyract-AP and F2000. Forty Class V cavity preparations were performed on extracted human teeth. As a negative control, twenty teeth were used without Class V preparations. The apical foramina of the teeth were sealed with a layer of varnish and amalgam restorations. Class V cavity preparations with occlusal margins in enamel, and gingival margins in cementum or dentin that measured approximately three millimeters in width (gingival-occlusal) and length (mesial-distal), were prepared on the buccal surface of the teeth. Samples were divided randomly into 2 groups of 15, and restored per manufacturer's instructions using experimental primer/conditioner (PCC, D/C) and Single Bond Adhesive (3M). All restorations were polished with an abrasive finishing kit. After storage in 37 degrees C water for 24 hours, all specimens were thermocycled between 5 degrees C and 55 degrees C for 500 cycles with a 30-second dwell time, followed by immersion in 0.2% basic fucsine for 24 hours. Teeth were then embedded in cold cure acrylic resin, sectioned longitudinally, and the dye penetration at the enamel and cementum margins were scored at 30x magnification. Evaluations were rated from 0 to 3 (0 = no leakage; 1 = dye penetration up to one-half of the preparation depth; 2 = dye penetration more than one-half preparation depth, but less than the axial wall; 3 = dye penetration along the axial wall). Both F-2000 and Dyract-AP indicated no leakage in the enamel margins. Dyract-AP showed no leakage at either the enamel or dentin margins. Fisher's Exact Test revealed that this difference in dentin margins was statistically significant (p < 0.05). Under the given conditions, Dyract-AP and F-2000 demonstrated resistance to microleakage in enamel, and showed Dyract-AP to be more resistant to microleakage in dentin than F-2000.     

Microleakage in MOD resin composite with three dentin bonding agents

The extent of microleakage under MOD composites was studied when an aluminum oxalate dentin bonding agent (Tenure), a phosphonated resin bonding agent (Bondlite), and a glass-ionomer cement (Ketac Silver) were used. Three groups of 10 extracted molars were prepared with MOD cavities; one box ended on enamel, the other on cementum. In Group 1, Bondlite was applied to dentin and etched enamel before the sample was restored with a light-cured hybrid composite. In Group 2, a 2-mm increment of Ketac Silver was placed in each box before Bondlite and composite. In Group 3, Tenure was applied to dentin before being restored. Teeth were thermal-cycled, stained in silver nitrate, sectioned, and scored for microleakage. Microleakage along the gingival floor was significantly less at enamel margins than at cementum margins in all three groups. All groups showed severe marginal microleakage on cementum.     

Microleakage study of flowable composite resin systems

The purpose of this in vitro microleakage study was to evaluate four low-viscosity composite resin systems. Each resin system included the corresponding bonding agent for each respective flowable composite. A hybrid material was used as a control. A total of 75 noncarious, freshly extracted human teeth were prepared with a Class 5 occlusal preparation and a gingival preparation at the cementoenamel junction. The materials were inserted according to the manufacturers' recommendations using the single-component bonding agent for each system. Teeth were thermocycled 800 times between 5 degrees C and 55 degrees C with 30-second dwell times. The teeth were then coated with nail polish 1 mm short of the restoration, placed in a basic fuchsin dye for 24 hours, and sectioned with a diamond wheel. Enamel and dentin/cementum margins were analyzed for microleakage on a scale of 0 (no leakage) to 3 (axial wall). Results were evaluated with the Fisher's exact test. The results of the study indicate that there was no leakage at the enamel margin of any restorations. No statistically significant differences in microleakage were found in dentin/cementum margins among the samples in the experimental group or between the experimental group and control group. The results indicate flowable composites demonstrate resistance to microleakage in both enamel and cementum/dentin margins similar to TPH hybrid composite.     

Evaluation of the cervical integrity during occlusal loading of Class II restorations

There are many concerns regarding the clinical behavior of packable composite restorations in Class II cavities, particularly when those restorations are subjected to axial mechanical loads. This study evaluated microleakage in vitro in proximal vertical "slot"-type cavities with walls located in enamel and dentin, filled with packable composite, associated or not associated with a flowable composite, a reinforced light-curing glass-ionomer or a compomer, after being submitted to occlusal load cycling. These preparations were subjected to either occlusal load cycling or no occlusal load cycling. Eighty human molars with enamel and dentin margins were treated with standardized cavity preparations (proximal vertical "slot" preparations). After completing the filling process using a packable composite (Filtek P60) with or without a cervical increment of flowable composite (Filtek flow), light-curing glass-ionomer (Vitremer) or compomer (Dyract AP), the molars were separated into two groups: control (without occlusal loading) and test, in which 4,000 one-second cycles of 150 N occlusal loading were applied. All 80 teeth were submitted to a microleakage test, then evaluated utilizing silver nitrate dye penetration. Significant statistical differences (Wilcoxon test, p<0.05) in the amount of leakage in enamel and dentin were found in both the control and test groups. After a paired comparison of the control and test groups, a significant statistical difference was found at the enamel level (Mann-Whitney test, p<0.05). In dentin, the only statistically significant difference found was the relation to the flow material. The Kruskal-Wallis test did not detect any statistically significant difference in the amount of leakage among the four materials studied, with a 5% level of significance for both enamel and dentin. Based on this data, it was concluded that restorations with margins located in dentin had greater microleakage than those restorations with margins located in enamel. When the samples were submitted to occlusal loading, they were negatively influenced, which increased microleakage values in enamel and dentin. There was no statistically significant difference among the four tested materials, when comparing their performance.     

Microleakage of various cementing agents for full cast crowns.

OBJECTIVES: To evaluate microleakage and marginal gaps in full cast crown restorations bonded with six different types of cementing agents. METHODS: Sixty non-carious human premolars and molars were prepared in a standardized manner for full cast crown restorations. The mesial and distal margins were located in dentin, while the vestibular and palatal/lingual margins were located in enamel. Crowns were made from a high-gold alloy using a standardized technique. The specimens were randomized to six groups of cementing agents: one zinc-phosphate cement (Harvard cement), one conventional glass-ionomer cement (Fuji I), one resin-modified glass-ionomer cement (Fuji Plus), two standard resin cements (RelyX ARC, Panavia F), and one self-adhesive universal resin cement (RelyX Unicem). After 4 weeks of storage in distilled water at 37 degrees C, the specimens were subjected to 5000 thermocycles ranging from 5 to 55 degrees C. Then, they were placed in a silver nitrate solution, embedded in resin blocks, and vertically cut in buccolingual and mesiodistal direction. Subsequently, the objects were evaluated for microleakage and marginal gap using a high-resolution digital microscope camera. RESULTS: A number of inter-group differences were statistically significant. RelyX Unicem showed the smallest degree of microleakage both in enamel and in dentin. Panavia F und RelyX Unicem were associated with significantly larger marginal gaps than all other cementing agents. No association was observed between microleakage and marginal gap other than a weak direct correlation when using Harvard cement on enamel. SIGNIFICANCE: The cementing agents investigated revealed different sealing abilities. These differences were not associated with specific types of materials.     

Microleakage around a low-shrinkage composite cured with a high-performance light

PURPOSE: To compare microleakage of Class V restorations filled with a novel low-shrinkage resin-based composite cured by a high intensity light and a combination of controls including a conventional composite cured by conventional and soft-start regimes. METHODS: Forty-two intact premolar and molar teeth were randomly assigned to seven groups and filled with a low-shrinkage composite or a conventional composite, bonded with one of two bonding materials (Excite or Prime&Bond NT) and cured with either a high intensity light, a soft-start regime or a conventional light regime. Cavities were cut in both the buccal and lingual aspects of each tooth with half of the preparation in enamel and half in dentin/cementum. The teeth were thermocycled and the restorations examined microscopically for leakage using Procion Brilliant Red as a marker. RESULTS: None of the groups showed microleakage at the enamel margins. All groups showed leakage at the gingival margins. At the gingival margins there was no significant difference between the low-shrinkage composite, bonded with the manufacturer's adhesive, and cured by high intensity regime, and the conventional composite bonded by the manufacture's adhesive and cured by one of two soft start regimes. Nor was there a significant difference between the low-shrinkage composite, bonded by the manufacturer's adhesive and cured by either the high intensity regime or one of two soft start regimes. The low-shrinkage material, bonded by the manufacturer's adhesive and cured by the high intensity regime showed significantly less microleakage than the conventional composite, bonded by the manufacturer's adhesive and cured with a conventional light source. CLINICAL SIGNIFICANCE: Gingival marginal microleakage is not prevented by the use of a low-shrinkage composite cured by a high intensity light. If time were an essential consideration, the low shrinkage composite cured by a high intensity regime may offer clinical advantages.     

An in vitro comparison of marginal microleakage of alternative restorative treatment and conventional glass ionomer restorations in extracted permanent molars

PURPOSE: The objective of this study was to compare the marginal leakage of cervical restorations made using alternative restorative treatment (ART) and conventional glass ionomer restorations. METHODS: Sixteen permanent maxillary and mandibular first and second molars extracted for periodontal reasons with Class V carious dentin on the buccal surfaces were prepared using ART while a second set of 29 noncarious molars had Class V preparations made with a high-speed handpiece. The occlusal margin was located in the enamel, and the gingival margin was located in the dentin/cementum. All teeth were restored with glass ionomer cement (GIC). The teeth were thermally stressed for 300 cycles and stained with methylene blue. Samples were sectioned and evaluated for microleakage. RESULTS: One-way analysis of variance on ranks revealed no significant difference in leakage at both the dentin and enamel margins between the conventional and ART groups. The microleakage at the dentin margin, however, was significantly greater (P < .001) than at the enamel margins in the conventional group. CONCLUSION: Alternative restorative treatment with GIC provides enamel and dentin margins that show comparable marginal leakage to conventionally restored permanent teeth. For the conventional restorations, leakage at the dentin margins occurs to a significantly higher extent than at the enamel margins.     

Marginal permeability of self-etch and total-etch adhesive systems

This study evaluated microleakage in vitro of self-etch and multi-step, total-etch adhesive systems. Ninety-six extracted non-carious human molars were randomly assigned to eight groups (n=12) and restored with different adhesive systems: Optibond Solo Plus, iBond, Adper Prompt L-Pop, Xeno III, Simplicity, Nano-Bond, Adper Scotchbond Multi-Purpose and Touch & Bond. Each group was treated following the manufacturer's instructions. Class V cavities were prepared on the facial or lingual surfaces of each tooth with coronal margins in enamel and apical margins in cementum (dentin). The teeth were restored with Z-100 resin composite. After polishing with Sof-Lex disks, the teeth were thermocycled for 1000 cycles and coated with nail varnish to within 1.0 mm of the restoration. The teeth were stained in 1% methylene blue dye for 24 hours and sectioned from the facial to lingual surface. Dye penetration (microleakage) was examined with a 20x binocular microscope. Enamel and dentin margin leakage was scored on a 0 to 3 ordinal scale. Data were analyzed using Kruskal-Wallis Analysis of Variance and Mann-Whitney U tests. Comparison of the adhesive groups at the enamel margin revealed: 1) Adper Scotchbond Multi-Purpose exhibited significantly less leakage than the other adhesive groups (except iBond); 2) among the self-etch adhesive groups, iBond exhibited significantly less leakage than Nano-Bond and 3) the other adhesive groups clustered intermediately. In contrast, there were no significant differences among the adhesive groups when the dentin margin was evaluated. A Wilcoxin signed rank test showed significantly less leakage at the enamel margins compared to the dentin margins of the eight adhesive systems tested. All data were submitted to statistical analysis at p<0.05 level of significance.     

The influence of matrix use on microleakage in Class 5 glass-ionomer restorations.

This in vitro study examined the relationship of matrix use to microleakage in class 5 Ketal-Fil glass-ionomer restorations. Class 5 glass-ionomer restorations were placed on the facial and lingual surfaces of 40 extracted human molars. The occlusal margin was located on enamel, and the cervical margin was located on cementum or dentin. Each tooth had one restoration placed with and without the aid of a soft metal matrix. Specimens were thermocycled (1234 cycles, 6 degrees C -60 degrees C, 30-second dwell time) and immersed in 5% methylene blue dye for four hours. The teeth were sectioned occlusogingivally through the center of each restoration, viewed with an optical microscope (X10), and each restoration was scored for dye penetration around the cavity walls. The enamel and cementum margins were scored separately for the extent of marginal leakage. No difference in leakage was found between restorations placed with or without a matrix (P > 0.05). Enamel restorations leaked significantly less than nonenamel margins, regardless of matrix use (P > 0.05).     

Microleakage patterns of porcelain and castable ceramic laminate veneers

This investigation evaluated the microleakage of conventional porcelain and Dicor castable ceramic laminate veneers bonded either entirely on acid-etched enamel or with the cervical margins on dentin. Three brands of light-activated composite resin cement systems were used and compared. Laminate veneers were bonded to extracted human maxillary central incisors and the extent of the marginal microleakage was measured. The findings indicated minimal marginal microleakage under both types of ceramic veneers bonded to all-enamel preparations. A markedly greater leakage at the dentin-composite resin interface was observed in veneers with cervical margins placed on dentin.     

The effect of different types of flowable restorative resins on microleakage of Class V cavities

This study evaluated the microleakage of Class V cavities restored with three different types of flowable resin restorative material and compared the effects of using their respective manufacturer's dentin adhesive or a different brand. Class V cavities with the occlusal margin in enamel and the gingival margin in dentin were prepared on the buccal and lingual surfaces of 48 non-carious human molars. The teeth were randomly assigned to three equal groups of 16. The first eight teeth in each group were restored with one of the flowable restorative materials (Filtek Flow, Dyract Flow, Admira Flow) using the manufacturer's recommended dentin adhesive (Single Bond, Prime & Bond NT, Admira Bond), and the remaining eight molars were restored using a different brand of dentin adhesive (Gluma Comfort Bond). The samples were thermocycled 200 times (5 degrees C-55 degrees C) with a one-minute dwell time. They were then immersed in a 2% basic fuchsin solution for 24 hours, sectioned and analyzed by stereomicroscopy. There was no statistically significant difference at the occlusal margins for either restoration used with its respective dentin adhesive. At the gingival margins, there was a significant difference among all groups. Flowable ormocer (Admira Flow/Admira Bond) displayed the least leakage at the gingival margins. When these flowable restoratives were used with a different brand of dentin adhesive, statistically significant differences were observed both on enamel and dentin. None of the restoratives tested fully prevented leakage at the gingival margins. No significant differences in microleakage were observed among the restorative materials used with respect to the manufacturer's dentin adhesive or a different brand except for Admira Flow restorative at the gingival margins. The gingival margins had significantly more microleakage than the occlusal margins (p < 0.05) except in the Admira Flow group, where microleakage at the occlusal and gingival margins was almost equal.     

Microleakage of seven adhesive systems in enamel and dentin

AIM: The aim of this study was to evaluate the microleakage of seven adhesive systems on two substrates (enamel and dentin). METHODS AND MATERIALS: Class V cavities were performed in buccal and lingual surfaces of 56 bovine incisors. The cervical margin was located in dentin and the incisal margin in enamel. The specimens were randomly divided into seven groups (n=16), according to the adhesive system employed: Single Bond; Excite; One Step Plus; Gluma One Bond; Magic Bond; One Up Bond F; and One Coat Bond. The cavities were incrementally filled with a hybrid composite Filtek Z250 and polymerized with a XL 3000 light curing unit. After polishing, the specimens were submitted to thermal cycling followed by dye immersion. Leakage was evaluated under magnification (40X) based on a standard ranking. Data were subjected to statistical analysis (Kruskal-Wallis). RESULTS: Enamel margins exhibited lower leakage than dentin margins (p<0.01). The majority of the specimens were leakage-free and materials performed similarly. Conversely, in dentin most of the specimens exhibited the highest leakage degree and significant differences among materials (p<0.05) were found, with Excite exhibiting the lowest leakage degree. It was concluded enamel provided better sealing and the adhesive system was a significant factor only in dentin.     

Influence of different restorative techniques on marginal seal of class II composite restorations

Objective

To evaluate the gingival marginal seal in class II composite restorations using different restorative techniques.

Material and Methods

Class II box cavities were prepared in both proximal faces of 32 sound human third molars with gingival margins located in either enamel or dentin/cementum. Restorations were performed as follows: G1 (control): composite, conventional light curing technique; G2: composite, soft-start technique; G3: amalgam/composite association (amalcomp); and G4: resin-modified glass ionomer cement/ composite, open sandwich technique. The restored specimens were thermocycled. Epoxy resin replicas were made and coated for scanning electron microscopy examination. For microleakage evaluation, teeth were coated with nail polish and immersed in dye solution. Teeth were cut in 3 slices and dye penetration was recorded (mm), digitized and analyzed with Image Tool software. Microleakage data were analyzed statistically by non-parametric Kruskal-Wallis and Mann-Whitney tests.

Results

Leakage in enamel was lower than in dentin (p<0.001). G2 exhibited the lowest leakage values (p<0.05) in enamel margins, with no differences between the other groups. In dentin margins, groups G1 and G2 had similar behavior and both showed less leakage (p<0.05) than groups G3 and G4. SEM micrographs revealed different marginal adaptation patterns for the different techniques and for the different substrates.

Conclusion

The soft-start technique showed no leakage in enamel margins and produced similar values to those of the conventional (control) technique for dentin margins.     

Microleakage of light-cured resin and resin-modified glass-ionomer dentin bonding agents applied with co-cure vs pre-cure technique

This in vitro study evaluated the effect of dentin bonding agents in reducing microleakage after three months in Class V restorations restored with Z100 resin composite. Materials tested were three types of resin-based dentin bonding agents: a multi-step (Scotchbond Multi-Purpose); a one-step (Scotchbond One-Step); a self-etching, self-priming (Clearfil Liner Bond) and a resin-modified glass ionomer (GC Fuji Bond LC). Class V cavity preparations with occlusal margins in enamel and gingival margins in cementum were prepared both on labial and lingual surfaces of extracted premolar teeth. Restorations (two per tooth) were distributed randomly into nine test groups (n = 10) consisting of the various DBAs applied with co-cure and pre-cure techniques, and no dentin bonding as a negative control group. Samples were stored in saline for three months, thermocycled, stained with silver nitrate, then sectioned through the middle of the preparation to facilitate the removal of the composite resin restoration. For groups treated with the pre-cure technique, the differences between the enamel leakage values of SBMP-control, CFLB-control and SB1S-control subgroups were significant (p < 0.05). For enamel leakage values of groups treated with the co-cure technique, the differences between the SBMP-control, SB1S-control, CFLB-control and Fuji LC-control subgroups were significant (p < 0.05). For cementum leakage values of groups treated with pre-cure technique, the difference between the CFLB-control and the Fuji, SBMP and SB1S groups was significant (p < 0.05). No significant differences could be detected between the cementum leakage values of groups treated with the co-cure technique (p > 0.05). The differences between the values obtained with application of CFLB with the pre-cure and co-cure techniques at the cementum margins were found to be statistically significant (p = 0.02). No statistically significant differences could be detected between the pre-cure and co-cure values of the other test materials. Generally for every group, cementum microleakage values were greater than enamel microleakage values (p < 0.05). The use of Scotchbond Multi-Purpose, Scotchbond One-Step and Fuji Bond LC with the co-cure technique to decrease the application time did not cause any significant increase in microleakage. Only pre-curing using Clearfil Liner Bond provided better microleakage properties than the other pre-cured adhesives.