腹腔镜阑尾切除与开腹阑尾切除术的对比研究及Meta分析

目的比较腹腔镜与开腹阑尾切除术对急性阑尾炎的疗效和手术安全性。方法收集2006年10月至2007年10月问120例急性阑尾炎患者进行前瞻性研究,随机分为两组,分别采用腹腔镜阑尾切除（LA）术式和开腹阑尾切除（OA）术式,对手术时间、术中出血量、术后胃肠功能恢复时间、止痛药物应用时间、开始进食时间、住院天数和术后并发症等指标进行比较分析。并对1996年1月至2008年12月期间的该类前瞻性随机对照研究（RCT）文献进行Meta分析,比较两种术式手术时间、住院天数和并发症等指标。结果本组资料表明,两组手术时间、出血量无明显差异,而LA组肠功能恢复时间、止痛药物应用时间、住院天数明显短于OA组,LA组手术并发症也少于OA组;Meta分析有6组资料纳入,LA住院天数和手术并发症少于OA组,而两组的手术时间无明显差异。结论腹腔镜阑尾切除术具有恢复时间短、术后疼痛轻、住院时间短和并发症发生率低等优点,值得推荐。     

阑尾切除常规手术与腹腔镜手术的临床比较

目的 探讨腹腔镜阑尾切除术（laparoscopic appendectomy，LA）与开腹阑尾切除术（open appendectomy，OA）优缺点，为选择正确的手术方法提供相关依据。方法 回顾性分析LA 53例与同期OA 64例，采用SPSS 13.0软件比较两组临床疗效。结果 LA 与OA除手术时间无明显差异外，LA组的住院时间、术后恢复时间、术后并发症等方面明显优于OA，P〈0.05。结论 LA与OA比较，具有明显的优势，可作为治疗急慢性阑尾炎的首选手术方式。     

腹腔镜复杂性阑尾切除术的体会

目的:探讨腹腔镜在复杂性阑尾切除术中的应用价值。方法:回顾分析1999～2009年施行907例复杂性阑尾切除术的临床资料,其中482例行腹腔镜阑尾切除术(laparoscopic appendectomy,LA),425例行传统开腹阑尾切除术(open ap-pendectomy,OA),对比分析两组手术情况及手术效果。结果:OA组在手术时间、住院费用方面优于LA组,但差异无统计学意义(P>0.05);在肛门恢复排气时间、住院时间、术后切口感染率、术后腹腔内残余脓肿发生率及术后粘连性肠梗阻发生率方面LA组优于OA组,差异有统计学意义(P<0.05);LA组术后肠梗阻形成时间优于OA组,但差异无统计学意义(P>0.05)。结论:与传统开腹阑尾切除术相比,LA在复杂性阑尾切除术中具有明显优势,具有术后肛门排气快、住院时间短、术后感染性并发症发生率低等优点,且并不明显增加住院费用及手术时间,可作为处理复杂阑尾炎时的首选术式。     

腹腔镜下阑尾手术与传统开腹手术的比较分析

目的 探讨腹腔镜阑尾切除术（LA）治疗急性阑尾炎的可行性及疗效。方法 32例急性阑尾炎行LA（LA组）和同期55例作传统开腹手术（OA）治疗（OA组）进行比较研究。结果 LA组病例术后愈合良好,未出现严重并发症,在术后止痛药使用率、术后下床活动时间、术后肛门排气时间、伤口感染率和住院时间方面有明显差异（P〈0.05）。结论 LA治疗急性阑尾炎是安全、可行的,相对于传统开腹手术有明显优势。     

腹腔镜下阑尾切除术治疗坏疽性阑尾炎

目的探讨腹腔镜下阑尾切除术治疗坏疽性阑尾炎的价值。方法回顾性分析113例坏疽性阑尾炎病人的临床资料,按手术方式分为腹腔镜下阑尾切除组(laparoscopic appendectomy,LA)及开腹阑尾切除组(open appendectomy,OA),分析两组间在一般临床资料、手术时间、腹腔引流管放置、术后疼痛评分(visual analogue scale,VAS)、术后进食时间、术后并发症(切口感染、腹腔脓肿、麻痹性肠梗阻)、住院时间等方面的差异。结果两组间在一般临床资料方面差异无统计学意义(P0.05)。在腹腔引流管放置、术后24小时疼痛评分(visual analogue scale,VAS)、术后并发症(切口感染、腹腔脓肿、麻痹性肠梗阻)方面差异具有明显统计学意义,LA组明显优于OA组(P0.05)。手术时间上OA略低于LA组,但两组未显示明显统计学差异(P=0.073)。两组在住院时间方面差异未显示出统计学意义(P=0.057)。结论腹腔镜下阑尾切除术治疗坏疽性阑尾炎与开腹手术相比具有术后并发症低、恢复快的优势,在有经验的医生可安全应用。     

腹腔镜手术和开腹手术治疗复杂阑尾炎的比较探讨

目的对比分析腹腔镜阑尾切除术（LA）与开腹阑尾切除术（OA）治疗复杂性阑尾炎的效果。方法复杂炎症者241例,LA手术152例,OA手术89例,中转开腹（CA）5例。结果LA组手术时间（114．5±42．6）min,术中出血量（30．2±24）ml,并发症发生率13．1％。OA组手术时间（89．7±41．2）min,术中出血量（81．2±92）ml,并发症发生率31．5％。两者在手术时间、术中出血量、术后并发症总发生率比较,差异有统计学意义（P〈0．05）;但在总住院费用方面差异没有统计学意义。结论复杂阑尾炎行LA,疗效可靠,安全可行。与开腹手术比较,具有术中出血少、术后总并发症少、住院时间短等优点。     

腹腔镜在急诊阑尾切除术中的应用

目的探讨腹腔镜在急诊阑尾切除术中的可行性及疗效。方法对200例急性阑尾炎随机分为传统的开腹阑尾切除术(Open appendectomy,OA)组100例和腹腔镜阑尾切除术(Laparoscopic appendectomy,LA)组100例,比较2组手术时间、住院时间、术后下床活动时间、术后切口感染等相关情况。结果所有患者均顺利完成手术,LA组有6例中转开腹,LA组住院时间、术后下床活动时间均少于OA组,差异有统计学意义(P<0.01),术后切口感染发生率LA组少于OA组,差异有统计学意义(P<0.05)。结论 LA具有创伤小、患者恢复快、并发症少、住院时间少等优点,是急诊阑尾炎患者可选用的有效手术方式。     

开腹和腹腔镜阑尾切除术治疗妊娠期急性阑尾炎的对照研究

目的 比较开腹阑尾切除术（open appendectomy,OA）和腹腔镜阑尾切除术（laparoscopic appendectomy,LA）治疗妊娠期急性阑尾炎的安全性及疗效.方法 回顾性分析我院2008年1月至2013年1月收治的68例妊娠早、中期急性阑尾炎的临床资料,其中OA组36例,LA组32例,并进行比对分析.结果 两组病例手术均顺利完成,OA组和LA组在妊娠相关并发症发生率上无明显差异（P＞0.05）;而在术后肛门排气时间、住院时间及术后并发症上,LA组均明显优于OA组,差异有统计学意义（P＜0.05）.结论 LA治疗妊娠早、中期急性阑尾炎是安全可行的,具有创伤小、术后恢复快、并发症少等优点.     

腹腔镜阑尾切除术的临床应用

目的对比腹腔镜阑尾切除术(LA)与开腹阑尾切除术(OA)的优缺点。方法回顾分析和对比近1年来106例腹腔镜阑尾切除术与同期施行的154例开腹阑尾切除术患者的临床资料。结果LA与OA的手术时间无明显差异(P>0.05),术后疼痛程度、术后住院时间、术后进食时间、术后下床时间、术后切口感染率、术后肠粘连发生率等方面LA明显优于OA(均P<0.05)。结论LA治疗急慢性阑尾炎较OA显示出明显优势,值得临床推广。     

急性阑尾炎行腹腔镜手术与开腹手术的比较

目的对比分析腹腔镜与开腹阑尾切除术在治疗急性阑尾炎中的手术效果。方法回顾性分析2009年1月至2011年10月58例行腹腔镜阑尾切除术（LA组）和同期55例行开腹阑尾切除术（OA组）的急性阑尾炎患者的临床资料,比较两种术式手术时间、下床活动时间、术后排气时间、止痛药物使用率、并发症发生率、住院时间及综合费用等指标。结果 LA组与OA组相比上述指标除手术时间外差异均有统计学意义（P〈0.05）。结论腹腔镜阑尾切除术治疗急性阑尾炎具有创伤小、恢复快、并发症少和住院时间短等优点,可作为治疗急性阑尾炎的理想选择。     

Vasopressor hormone response following mesenteric traction during major abdominal surgery

Background : We investigated the vasopressor hormone response following mesenteric traction (MT) with hypotension due to prostacyclin (PGI₂) release in patients undergoing abdominal surgery with a combined general and epidural anesthesia. Methods : In a prospective, randomized, placebo-controlled study we administered 400 mg ibuprofen (i.v.) in 42 patients scheduled for abdominal surgery. General anesthesia was combined with epidural anesthesia (T4-L1). Before as well as 5, 15, 30, 45, and 90 min after MT we recorded plasma osmolality, hemodynamics and measured 6-keto-PGFlα (stabile metabolite of PGI₂), TXB₂ (stabile metabolite of thromboxane A₂) active renin, and arginine vasopressin (AVP) plasma concentrations by radioimmunoassay. Catecholamine levels were assessed by high-pressure liquid chromatography (HPLC) with electrochemical detection. Results : Following MT, arterial hypotension occurred along with a substantial PGI₂ release. This was completely abolished by ibuprofen administration. Although plasma levels of 6-keto-PGF_1α (1133 (708) vs. 60 (3) ng/L, median (median absolute deviation), P=0.0001, placebo vs. ibuprofen) remained significantly elevated, blood pressure was restored within 30 min after MT in the placebo group. At the same point in time plasma concentrations of TXB² (164 (87) vs. 58 (1) ng/L, P=0.0001), epinephrine (46 (33) vs. 14 (6) ng/L, P=0.001), AVP (41 ± (18) vs. 12 (7) ng/L, P=0.0004), and active renin (27 (12) vs. 12 (4) ng/L, P = 0.001) were significantly higher in placebo-treated patients. Conclusion : Under combined general and epidural anesthesia arterial hypotension following MT due to endogenous PGI₂ release is associated with enhanced release of AVP, active renin, epinephrine and thromboxane A₂, presumably contributing to hemodynamic stability within 30 min after MT.     

A Teaspoon Pump for Pumping Blood with High Hydraulic Efficiency and Low Hemolysis Potential

Abstract: Virtually all blood pumps contain some kind of rubbing, sliding, closely moving machinery surfaces that are exposed to the blood being pumped. These valves, internal bearings, magnetic bearing position sensors, and shaft seals cause most of the problems with blood pumps. The original teaspoon pump design prevented the rubbing, sliding machinery surfaces from contacting the blood. However, the hydraulic efficiency was low because the blood was able to "slip around" the rotating impeller so that the blood itself never rotated fast enough to develop adequate pressure. An improved teaspoon blood pump has been designed and tested and has shown acceptable hydraulic performance and low hemolysis potential. The new pump uses a nonrotating "swinging" hose as the pump impeller. The fluid enters the pump through the center of the swinging hose; therefore, there can be no fluid slip between the revolving blood and the revolving impeller. The new pump uses an impeller that is comparable to a flexible garden hose. If the free end of the hose were swung around in a circle like half of a jump rope, the fluid inside the hose would rotate and develop pressure even though the hose impeller itself did not "rotate"; therefore, no rotating shaft seal or internal bearings are required.     

Microspheres Based Detoxification System: A New Method in Convective Blood Purification

Abstract: A variety of protein-bound or hydrophobic substances, accumulating as a result of pathologic conditions such as exogenous or endogenous intoxications, are removed poorly by conventional detoxification methods because of low accessibility (hemodialysis), insufficient adsorption capabilities (hemosorption), low efficiency (peritoneal dialysis), or economic limitations (high-volume plasmapheresis). Combining advantages of existing methods with microspheric technology, a module-based system was designed. Major operating parameters of the latter can be modified to allow for adjustment to individual clinical situations. An extracorporeal blood circuit including a plasmafilter is combined with a secondary high-velocity plasma circuit driven by a centrifugal pump. Different microspheric adsorbers can be combined in one circuit or applied in sequence. Thus, a prolonged treatment can be tailored using specially designed selective adsorber materials. Comparing this system with existing methods (high-flux hemodialysis, molecular adsorbent recycling system), results from our in vitro studies and animal experiments demonstrate the superior efficiency of substance removal.     

Tissue perfusion in relation to cardiac output during continuous positive-pressure ventilation and administration of propranolol or verapamil

Background : Our objective was to determine whether administration of propranolol or verapamil modifies the hemodynamic adaptation to continuous positive-pressure ventilation (CPPV), in particular the regional distribution of cardiac output (CO).
Methods : General hemodynamics and regional blood flows assessed by microsphere technique (15 (μm) were recorded in 16 anesthetized pigs during spontaneous breathing (SB) and CPPV with 8 cm H₂O end-expiratory pressure (CPPV8) before and after intravenous administration of propranolol (0.3 mg · kg⁻¹ followed by 0.15 mg · kg⁻¹ · h⁻¹, n=8) or verapamil (0.1 mg · kg⁻¹ followed by 0.3 mg · kg⁻¹ · h⁻¹, n=8).
Results : CPPV8 depressed CO by 25% without shifts in its relative distribution with the exception of a noteworthy increase in adrenal perfusion. Propranolol increased arterial blood pressure, and due to a fall in heart rate, CO dropped by 25%. The kidneys and, to a lesser extent, the splanchic region and central nervous system received increased fractions of the remaining CO at the expense of skeletal muscle flow. Similar patterns were seen during SB and CPPV8 such that the combination of propranolol and CPPV8 depressed CO by 50%. The circulatory effects of verapamil were less evident but myocardial perfusion tended to increase.
Conclusions : The combination of propranolol or verapamil with CPPV does not result in any specific hemodynamic interaction in anesthetized pigs, except that the combined effect of propranolol and CPPV may severely reduce CO.     

Volatile anaesthetics reduce adhesion of blood platelets under low-flow conditions in the coronary system of isolated guinea pig hearts

Background : Inhibitory effects of volatile anaesthetics on platelet aggregation have been demonstrated in several studies. However, the influence of volatile anaesthetics on intracoronary platelet adhesion has not been elucidated so far.
Methods : Isolated hearts of guinea pigs were perfused with buffer in the absence or presence of volatile anaesthetics (0.5 and 1 MAC) at constant coronary flow rates of 5 ml/min for 25 min, then 1 ml/min for 30 min and again 5 ml/min for 10 min. Before, during and after low-flow perfusion, a bolus of human platelets was applied into the coronary system. To simulate thrombogenic conditions, 0.3 U/ml human thrombin was infused during low-flow perfusion and reperfusion. The number of platelets sequestered to the endothelium was calculated from the difference between coronary in- and output of platelets. The myocardial production of lactate and consumption of pyruvate and coronary perfusion pressure were also determined.
Results : At a flow rate of 5 ml/min only about 3% of the applied platelets did not emerge from the coronary system, in any group. In contrast, 13.1±1.2% (mean±SEM) of infused platelets became adherent in low-flow perfusion in the control group without anaesthetic. The adherence was reduced with each 1 MAC isoflurane (to 6.2±1.2%), sevoflurane (to 4.4±0.9%) or halothane (to 3.2±1.5%) (each P <0.05 vs. control). Volatile anaesthetic, 0.5 MAC, did not inhibit platelet adhesion to a statistically significant extent in any case. Perfusion pressure and metabolic parameters were not statistically different between the control and the hearts exposed to anaesthetics.
Conclusion : Volatile anaesthetics in a concentration of 1 MAC can reduce the adhesion of platelets in the coronary system under reduced flow conditions. This action does not arise from vasodilation or inhibition of ischaemic stress.     

Bariatric Surgery in Some "Central and East-European (former Eastern bloc)"Countries - Current Status and Prediction for the Next Millennium

Background: Obesity is increasing globallly, including in the formerly "Eastern Bloc" countries. Methods: A survey was made of obesity and bariatric surgery. Results: In the 8 East and Central European countries studied, with total population 300 million, roughly 43% of the population was overweight (BMI 25-30), 23% obese (BMI > 30), with about 15 million people morbidly obese (BMI > 40). From 0-10 morbidly obese individuals/100,000/year undergo bariatric surgery. Conclusion: Most countries were found to provide inadequate treatment for obesity.The majority of the morbidly obese are not treated effectively. However, health-care awareness of obesity and bariatric surgeons are slowly increasing.     

Is the recovery profile of mivacurium independent of the rate of decay of its plasma concentration in patients with normal plasma cholinesterase activity?

Background: The duration of action of muscle relaxants is poorly correlated to the rate of decay of their plasma concentration. The plasma concentration of mivacurium may rapidly decrease below its active concentration because of the extensive hydrolysis of mivacurium. By inflating a tourniquet on one upper limb for 3 min after the administration of atracurium, mivacurium or vecuronium, we studied the influence of the initial decline of their plasma concentration on their effect. Methods: In 50 patients anaesthetised with thiopental, isoflurane and fentanyl, the effect of bolus doses of 0.15 or 0.25 mg . kg^?1 mivacurium (MIV 15, MIV 25), 0.3 or 0.5 mg . kg^?1 atracurium (ATR 30, ATR 50) and 0.06 or 0.1 mg . kg^?1 vecuronium (VEC 06, VEC 10) were measured on both arms (evoked response of the adductor pollicis to train-of-four stimulation every 12 s), a tourniquet being applied on one arm just before and during 3 min after the muscle relaxant bolus. Results: Tourniquet inflation of 3 min almost abolished the neuromuscular effect of mivacurium. In the vecuronium groups and in the ATR 50 group, tourniquet inflation did not modify the maximum degree of depression of the twitch response. Also, the duration of action of vecuronium was unaffected by the tourniquet. In the ATR 30 group, times to return of the twitch response to 25% (duration 25%) and 75% (duration 75%) of control response were significantly shorter in the cuffed arm, 23 min vs 27 min, and 41 min vs 45 min, respectively. In the ATR 50 group, only duration 25% was significantly shorter in the cuffed arm (41 min vs 45 min). Conclusion: The results suggest that the rate of decline of the plasma concentration of mivacurium is so rapid, that a very low and almost clinically ineffective concentration is present as soon as 3 min after its administration. The results also indicate that the recovery from a mivacurium-induced neuromuscular blockade is not influenced by the rate of decay of its plasma concentration in patients with genotypically normal plasma cholinesterase.     

Membranes in Artificial Organs

Abstract: Membrane processes play a pivotal and enabling role in modern replacement therapy for acute and chronic organ failure and in the management of immunologic diseases. In fact, virtually all contemporary extracorporeal blood purification methods employ membrane devices, and the next generation of artificial organs and tissue engineering therapies are almost certain to be similarly grounded in membrane technology. In this short essay, we comment on the similarities and differences among synthetic membranes and their natural counterparts and also provide a critical overview of the demographics and technology of hemodialysis, hemofiltration, apheresis, oxygenation, and emerging membrane technologies and applications.     

Influence of dopexamine hydrochloride on haemodynamics and regulators of circulation in patients undergoing major abdominal surgery

Background: Catecholaminergic support is often used to improve haemodynamics in patients undergoing major abdominal surgery. Dopexamine is a synthetic vasoactive catecholamine with beneficial microcirculatory properties. Methods: The influence of perioperative administration of dopexamine on cardiorespiratory data and important regulators of macro- and microcirculation were studied in 30 patients undergoing Whipple pancreaticduodenectomy. The patients received randomized and blinded either 2 μg · kg^?1 · min^?1 of dopexamine (n=15) or placebo (n=15, control group). The infusion was started after induction of anaesthesia and continued until the morning of the first postoperative day. Endothelin-1 (ET-1), vasopressin, atrial natriuretic peptide (ANP), and catecholamine plasma levels were measured from arterial blood samples. Measurements were carried out after induction of anaesthesia, 2 h after onset of surgery, at the end of surgery, 2 h after surgery, and on the morning of the first postoperative day. Results: Cardiac index (CI) increased significantly in the dopexamine group (from 2.61±0.41 to 4.57±0.78 1 · min^?1 · m^?2) and remained elevated until the morning of the first postoperative day. Oxygen delivery index (DO₂I) and oxygen consumption index (VO₂I) were also significantly increased in the dopexamine group (DO₂I: from 416±91 to 717±110 ml/m² · m²; VO₂I: from 98±25 to 157±22 ml/m² · m²), being significantly higher than in the control group. pHi remained stable only in the dopexamine patients, indicating adequate splanchnic perfusion. Vasopressive regulators of circulation increased significantly only in the untreated control patients (vasopressin: from 4.37±1.1 to 35.9±12.1 pg/ml; ET-1: from 2.88±0.91 to 6.91±1.20 pg/ml). Conclusion: Patients undergoing major abdominal surgery may profit from prophylactic perioperative administration of dopexamine hydrochloride in the form of improved haemodynamics and oxygenation as well as beneficial influence on important regulators of organ blood flow.     

Synergistic interaction between the effects of propofol and midazolam with fentanyl on phrenic nerve activity in rabbits

Background: It has been shown that the depressive effects of both propofol and midazolam on consciousness are synergistic with opioids, but the nature of their interactions on other physiological systems, e. g. respiration, has not been fully investigated. The present study examined the effect of propofol and midazolam alone and in combination with fentanyl on phrenic nerve activity (PNA) and whether such interactions are additive or synergistic. Methods: PNA was recorded in 27 anaesthetised and artificially ventilated rabbits. In three groups, propofol, fentanyl and midazolam were administered intravenously in incremental doses to construct dose-response curves for the depressant effects of each one on PNA. In another two groups, the effect of pretreatment with either fentanyl 1 μg · kg^?1 i. v. or midazolam 0.05 mg · kg^?1 i. v. on the effects of propofol and fentanyl respectively on PNA were studied. Results: Propofol and fentanyl caused a dose-dependent depression of PNA with complete abolition at the highest total doses of 16 mg · kg^?1 i. v. and 32 μg · kg^?1 i. v., respectively. In contrast, midazolam in incremental doses to a total of 0.8 mg · kg^?1 reduced mean PNA by 63%, but approximately 12% of PNA remained at a total dose as high as 6.4 mg · kg^?1. The mean ED₅₀s, calculated from dose-response curves, were 5.4 mg · kg^?1, 3.9 μg · kg^?1 and 0.4 mg · kg^?1 for propofol, fentanyl and midazolam, respectively. Initial doses of either fentanyl 1 μg · kg^?1 i. v. or midazolam 0.05 mg · kg^?1 i. v. acted synergistically with subsequent doses of either propofol or fentanyl to abolish PNA at total doses of 8 mg · kg^?1 and 8 μg · kg^?1, respectively. Conclusion: Fentanyl has a synergistic interaction with both propofol and midazolam on PNA and hence potentially on respiration.