荔枝核与龙眼核水提取物的体外抗肿瘤活性的比较与评估

目的:观察比较荔枝核与龙眼核水提取物的抗肿瘤活性.方法:体外培养CNE-2Z、CNE1、Huh7、A549等4种肿瘤细胞,通过MTT法检测了该两种水提取物对以上4种肿瘤细胞的细胞增殖抑制作用.结果:荔枝核与龙眼核水提取物对于4种肿瘤细胞的增殖有不同程度的抑制作用,其中荔枝核水提取物对于鼻咽癌细胞株CNE-2Z与CNE1抑制作用较为突出.龙眼核与荔枝核水提取物作用细胞48h后的抑制作用与作用24h有显著差异(P＜0.05).结论:荔枝核与龙眼核水提取物均具抗肿瘤活性.     

龙眼核醋酸乙酯部位的抗氧化活性研究

目的研究龙眼（Dimocarpus longan lour.）核醋酸乙酯部位的抗氧化活性。方法通过乙醇提取、醋酸乙酯溶剂萃取、硅胶柱层析等方法，分离提取不同的极性组分（Fr1～Fr16）。运用TBA比色法分别测定了Fr8～Fr16各组分的抗脂质过氧化活性、应用Fenton原理测定了各组分清除羟自由基的活性。结果分离提取得到16个极性不同的组分（Fr1～Fr16），流分Fr8～Fr16对抑制脂质过氧化物、清除羟自由基均具有较好作用，其中部分流分的活性具有量-效关系。最高的抑制率和清除率可达到68．5％。结论龙眼核醋酸乙酯部位的不同流分均具有一定的抑制脂质过氧化作用和清除羟氧自由基作用。     

金鸡菊提取物体外抗氧化活性

目的:探讨金鸡菊提取物体外抗氧化活性。方法:采用分光光度法,测定金鸡菊提取物对羟基(OH)自由基、超氧阴离子(O 2-.)自由基及1,1-二苯基-2-苦肼基自由基(DPPH)的清除能力,通过多元线性回归分析,考察金鸡菊提取物含量及其体外抗氧化活性的相关性,并同维生素(VC)进行了比较。结果:金鸡菊提取物对.OH自由基,O 2-.自由基,DPPH自由基均有较好的清除作用,在供试质量浓度范围内(0.2～1.0 g.L-1)对各自由基的清除效率与金鸡菊提取物浓度有一定量效关系,当质量浓度达1.0 g.L-1,其中50%乙醇洗脱物对OH自由基、O 2-.自由基和DPPH自由基的清除率可达86.19%,97.90%和71.85%,且清除能力与Vc相当。结论:金鸡菊提取物具有显著的体外抗氧化作用。     

杨梅核提取物的体外抗氧化活性研究

目的:考察杨梅核不同溶剂提取物的体外抗氧化活性。方法:采用分光光度法,测定杨梅核不同溶剂提取物对DPPH、脂质过氧化的清除能力和总还原力,考察了杨梅核提取物体外抗氧化活性。结果:杨梅核不同溶剂提取物具有较强的DPPH、脂质过氧化的清除能力和总还原力,其中杨梅核无水乙醇提取物的体外抗氧化作用最强。结论:杨梅核提取物具有显著的抗氧化活性。     

荔枝核水提物降血糖作用的实验研究

荔枝核的降糖机制与双胍类降糖药物相似.用链脲霉素制造糖尿病小鼠模型,研究荔枝核水提物对正常小鼠及模型鼠降糖作用.结果显示荔枝核水提物能显著降低模型小鼠的血糖水平,但对正常小鼠不起作用.指出,荔枝核兼具磺胺类及双胍类降糖药效的结论略有差异.     

贯叶连翘标准提取物体外抗氧化活性

贯叶连翘Hypericum perforatum L．为藤黄科金丝桃属植物，又名千层楼、圣约翰草等，地上部分或全草入药，其性平味涩，有清热解毒、收敛止血、利湿之功效。其主要成分有苯并二蒽酮类、黄酮类、挥发油类和间苯三酚类。贯叶连翘富含黄酮类化合物，高达11．7％，主要为金丝桃苷、槲皮素、槲皮苷及芦     

藏紫菀不同溶剂提取物的体外抗氧化活性

目的 研究藏紫菀不同溶剂提取物的体外抗氧化活性.方法 采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法、2,2'-联氮基双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除法、超氧阴离子自由基清除法、NO自由基清除法、羟自由基清除法及铁还原/抗氧化能力(FRAP)法,对藏紫菀70％乙醇粗提物、正己烷...     

荞麦皮提取物抗氧化活性的研究

植物中普遍含有的酚类化合物具有抗氧化作用。荞麦在日本被认为是一种健康食品,主要成分为芦丁。每年消耗大量荞麦的     

黄芪提取物免疫调节活性的体外实验研究

目的：研究黄芪提取物（Astragalus membranaceus extract,AME)对人的外周血免疫细胞（PBIC）免疫功能的调节作用。方法：采用^3H-TdR掺入法分析AME对外周血单个核细胞（PBMC）的增殖活性及对外周血粘附单核细胞（PBAM）吞噬肿瘤细胞的影响；采用^51Cr释放法测定AME对杀伤性T细胞（CTL）杀伤肿瘤细胞的影响；用ELISA法和生物学法研究AME对外周血B细胞（PBBC）产生IgG及对PBAM产生细胞因子的影响；采用SDS－PAGE分析AME的蛋白组成。结果：AME能促进PBMC的增殖；提高CTL对肿瘤细胞的杀伤活性；增强PBAM对肿瘤细胞的吞噬和产生细胞因子的功能；促进了PBBC产生IgG;AME含有多种蛋白成分。结论：AME对人免疫功能有增强作用，提高了抗肿瘤免疫效应，可应用于临床调节免疫功能和治疗肿瘤等疾病。     

苍耳七提取物的体外抗氧化活性研究(英文)

目的:了解苍耳七不同提取物中总黄酮含量、抗氧化活性及黄酮含量与抗氧化活性之间的相关性。方法:用三种不同有机溶剂依次对苍耳七 50 %乙醇提取物进行萃取,分别得到氯仿提取物(CE)、乙酸乙酯提取物(EAE)、正丁醇提取物(BE)和水相提取物(WE)。总黄酮含量采用 NaNO2- Al (NO3)3-NaOH 显色法测定。抗氧化活性采用羟基自由基清除试验、超氧自由基清除试验、DPPH 自由基清除试验、还原力测定和金属离子螯合试验进行综合评价,以 BHT(2, 6-二叔丁基对甲酚)为参考物质。结果:EAE 的黄酮含量最高,其次分别为 BE, WE 和 CE。WE 和 BE 的抗氧化活性强于 CE 和 EAE. 每种提取物的抗氧化活性都显示出浓度依赖性和黄酮含量的相关性。结论:苍耳七提取物可以被用作天然抗氧剂。     

菘蓝种子总多酚提取工艺的优化及抗氧化活性研究

目的研究菘蓝种子总多酚的最佳提取工艺条件,并初步探讨其抗氧化活性。方法采用单因素试验和L9(34)正交试验法,确定菘蓝种子总多酚最佳提取工艺;并考察其对DPPH.的清除能力。结果菘蓝种子总多酚的最佳提取工艺为:料液比1︰10,甲醇质量分数60%,提取温度50℃,提取时间40 min。抗氧化活性评价显示菘蓝种子总多酚清除DPPH.的能力呈浓度依赖效应,当总多酚质量浓度为1.0μg/mL时,与BHT的抗氧化能力基本相当。结论本研究建立了菘蓝种子总多酚的最佳超声提取工艺条件,总多酚得率为(8.92±0.03)mg/g,DPPH.法评价结果显示其具有一定的抗氧化活性。     

苍耳子4种提取物小鼠急性毒性比较研究

目的:研究苍耳子生品和炒品的水提、醇提4种提取物对小鼠的急性毒性。方法:观察小鼠灌胃给药后的毒性反应,并计算半数致死量(LD50)。结果:苍耳子水提取物组毒性反应为静卧、竖毛、尾足发绀、震颤、呼吸抑制、间歇性惊厥、翻正反射消失、后腿抽搐等,乙醇提取物组则表现为静卧、腹式呼吸、部分竖毛、震颤、严重间歇性惊厥、跳跃、后腿抽搐,部分动物出现大小便失禁等。苍耳子炒品水提取物、炒品乙醇提取物、生品水提取物、生品乙醇提取物的小鼠灌胃给药的LD50分别为生药155.93,317.80,167.60,275.41 g.kg-1。结论:苍耳子水提取物的急性毒性明显大于乙醇提取物,而炒品和生品的毒性差异不明显。     

Preliminary observations on the antifatigue effects of longan (Dimocarpus longan Lour.) seed polysaccharides

The antifatigue effects of the hot‐water extract of longan (Dimocarpus longan Lour.) seeds were studied in mice. Longan seed polysaccharides were administered at doses of 50, 100, 200 and 400 mg/kg and antifatigue activity was evaluated using a swimming test, along with the determination of serum urea nitrogen, hepatic glycogen and blood lactic acid content. The results show that longan seed polysaccharides, in doses ranging from 50 to 100 mg/kg, extended swimming time, increased hepatic glycogen (p < 0.01, n = 10), reduced blood urea nitrogen (p < 0.01, n = 10) and decreased blood lactic acid (p < 0.01, n = 10) in the mice. Therefore longan seed polysaccharides may have potential as an antifatigue agent. Copyright © 2010 John Wiley & Sons, Ltd.     

银杏叶提取物双重抗氧化作用的研究

目的:抗氧化物质一般通过诱导抗氧化酶的表达或直接与自由基发生反应这两种方式发挥作用,银杏叶提取物(EGb)的抗氧化作用是通过哪种途径值得研究.在本研究中,我们在体外对两种途径进行了测试.方法:EGb作用于细胞,用Western-blot检测其对抗氧化酶GST-P1和GCLC的诱导作用;用相应的功能检测法测定EGb在体外直接清除超氧阴离子及羟自由基、抑制大鼠红细胞溶血及大鼠肝组织的脂质过氧化的作用.结果:EGb可诱导抗氧化酶GST-P1和GCLC的表达,并能直接清除超氧阴离子及羟自由基、抑制大鼠红细胞溶血及大鼠肝组织的脂质过氧化.结论:EGb具有诱导抗氧化酶表达及直接清除自由基的双重抗氧化作用.     

Oral intake of proanthocyanidin-rich extract from grape seeds improves chloasma

Chloasma (melasma), an acquired hypermelanosis, is often recalcitrant to various treatments and an amenable, as well as safe, pigment-reducing modality is needed. We investigated that the reducing effect of proanthocyanidin, a powerful antioxidant, on chloasma in a one-year open design study. Proanthocyanidin-rich grape seed extract (GSE) was orally administered to 12 Japanese woman candidates with chloasma for 6 months between August 2001 and January 2002 and to 11 of these 12 for 5 months between March and July 2002. Clinical observation, L* value (lightening) and melanin index, and size (length and width) measurements of chloasma were performed throughout the study period. The first 6 months of GSE intake improved or slightly improved chloasma in 10 of the 12 women (83%, p < 0.01) and following 5 months of intake improved or slightly improved chloasma in 6 of the 11 candidates (54%, p < 0.01). L* values also increased after GSE intake (57.8 +/- 2.5 at the start vs 59.3 +/- 2.3 at 6 months and 58.7 +/- 2.5 at the end of study). Melanin-index significantly decreased after 6 months of the intake (0.025 +/- 0.005 at the start vs 0.019 +/- 0.004 at 6 months) (p < 0.01), and also decreased at the end of study (0.021 +/- 0.005) (p < 0.05). GSE is effective in reducing the hyperpigmentation of women with chloasma. The beneficial effects of GSE was maximally achieved after 6 months and these was no further improvement after this period. The latter GSE intake for 5 months may prevent chloasma from becoming worse prior to the summer season. GSE is safe and useful for improving chloasma.     

银杏叶及葡萄籽提取物体外抑制蛋白糖化终末产物生成作用的研究

 目的 应用体外蛋白糖化反应系统,确定银杏叶及葡萄籽提取物抑制蛋白糖化终末产物生成的作用。方法 对照组将葡萄糖与牛血清白蛋白分别在STUOX；条件下共同孵育，实验组则加入不同剂量的银杏叶及葡萄籽提取物或氨基胍。利用荧光分光光度计对不同温度和时间培养条件下的样品测定，根据荧光强度确定蛋白糖化终末产物的生成量。结果 在本体外系统中，蛋白糖化终末产物的生成与孵育温度及时间呈正相关。银杏叶提取物及葡萄籽提取物在1.0~2.0 g.L_-1剂量范围内均可有效抑制蛋白糖化终末产物的生成，当药物浓度达2.0 g·L_-1时其抑制作用相当于同剂量的氨基胍。结论 具有明确 抗氧化作用的银杏叶提取物及葡萄籽提取物在体外可有效抑制蛋白糖化终末产物的生成。     

Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs

The water phase antioxidant activity of extracts from 23 Bulgarian medicinal plants was studied in relation to their polyphenol content in comparison with mate, black tea, honeybush and rooibos foreign species. Antioxidant activity was measured by the ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) cation radical decolorization assay, and the total polyphenol content was assayed according to the Folin-Ciocalteu method. Five Bulgarian plant extracts exhibited higher antioxidant activity than that of mate, which is 21.7% of all Bulgarian herbs included in this study. These were Alchemilla vulgaris L. (4.79 +/- 0.14 mm), Sambucus ebulus L. (4.03 +/- 0.07 mm), Mentha spicata L. (3.90 +/- 0.03 mm), Fragaria vesca L. (3.74 +/- 0.06 mm), Crataegus monogyna Jacq. (3.63 +/- 0.05 mm). Another eight Bulgarian medicinal plant extracts exhibited an intermediate antioxidant activity - lower than that of mate and higher than that of honeybush, which makes 34.8% of all Bulgarian herbs included in the study. More than half of the herbal extracts included in the present study exhibited antioxidant activity higher than or comparable to the reference foreign plants. A positive correlation (r = 0.92) between antioxidant activity and polyphenol content was found, suggesting that the antioxidant capacity of the aqueous plant extracts is due to a great extent to their polyphenols.     

Cholinesterases inhibitory and antioxidant activities of Harpagophytum procumbens from in vitro systems

A previous report showed that extracts of cell suspension and transformed root cultures of Harpagophytum procumbens (commonly known as Devil's claw), an African plant with high medicinal value, exhibit strong antiinflammatory characteristics. The present work tests the ability of extracts, phenylethanoid-containing fractions and the major phenylethanoid glycoside isolated from the Devil's claw cultures, to inhibit acetylcholinesterase and butyrylcholinesterase, and the antioxidant activity in iron-related systems (e.g. ferric-reducing antioxidant power and ferrous ion-chelating capacity). The results indicated that the phenylethanoid fractions may be attractive for various commercial purposes since they displayed significant cholinesterase inhibitory activity (even higher than that of pure galanthamine in the case of butyrylcholinesterase inhibition assay). Crude methanolic extracts from cell and hairy root cultures of Devil's claw exhibited strong ferrous ion-chelating capacity (1.5-2 times higher than pure butylated hydroxyanisole, used as positive standard).     

In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves

The in vitro antiproliferative and antioxidant activities of the aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extracts of M. calabura inhibited the proliferation of MCF-7, HeLa, HT-29, HL-60 and K-562 cancer cells while the chloroform extract only inhibited the proliferation of MCF-7, HeLa, HL-60 and K-562 cancer cells. Interestingly, all extracts of M. calabura, which failed to inhibit the MDA-MB-231 cells proliferation, did not inhibit the proliferation of 3T3 (normal) cells, indicating its safety. All extracts (20, 100 and 500 μg/ml) were found to possess antioxidant activity when tested using the DPPH radical scavenging and superoxide scavenging assays with the methanol, followed by the aqueous and chloroform, extract exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 2970.4 ± 6.6, 1279.9 ± 6.1 and 2978.1 ± 4.3 mg/100 g gallic acid, respectively. In conclusion, the M. calabura leaves possess potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds, and thus, needs to be further explored.     

蒲葵籽提取物体外抗HIV-1活性及机制的初步研究

目的研究蒲葵籽提取物的体外抗HIV-1活性,对有活性粗提物进行初步机制研究。方法采用细胞毒性、合胞体抑制、HIV-1感染细胞保护实验和HIV-1 p24抗原测定等实验对蒲葵籽提取物体外抗HIV-1活性进行筛选和确认;采用重组HIV-1逆转录酶和蛋白酶活性抑制实验,融合阻断实验初步探讨活性粗提物的作用机制。结果蒲葵籽的醋酸乙酯(P3)提取物具有较强的体外抗HIV-1活性,P3抑制HIV-1诱导C8166细胞形成合胞体的EC50为5.64μg/mL,对C8166细胞的毒性较小,CC50大于200μg/mL,治疗指数(TI)大于35.46;P3抑制HIV-1急性感染中p24抗原表达的EC50为23.04μg/mL,抑制正常C8166细胞与慢性感染细胞H9/HIV-1 B融合的EC50为8.00μg/mL;P3在质量浓度为200μg/mL时,对HIV-1逆转录酶的抑制率为28.86%;P3抑制重组HIV-1蛋白酶活性的EC50为1.77μg/mL。结论蒲葵籽的醋酸乙酯提取物(P3)具有较强的体外抗HIV-1活性,其作用机制可能主要为阻断病毒进入和抑制HIV-1蛋白酶活性。