实验性大鼠腮腺导管细胞缺血再灌注TGF-β1的表达

目的 探讨TGF—β1(Transfoming growth factor—β1—转化生长因子—β1)在实验性大鼠腮腺导管细胞的表达变化与形态学改变的关系。方法 采用颈总动脉结扎法建立腮腺缺血再灌注模型，用光镜、免疫组化的方法，观察腮腺导管细胞形态学及TGF—β1的表达变化。结果 实验组在缺血再灌注早期，开始出现形态学改变，中期明显，后期渐修复。缺血时间相同时，随再灌注时间的延长，TGF—β1的表达先升高后降低。再灌注时间相同时，随缺血时间的延长，细胞TGF—β1的表达有明显增高趋势。结论 实验性缺血再灌注大鼠腮腺导管细胞有形态学改变，TGF—β1的表达在腮腺缺血再灌注损伤早期增加，可能起到抑制细胞增殖的作用，以后逐渐减弱，不是影响细胞结构变化的主要因素；缺血时间越长，TGF—β1的表达增加，提示细胞增殖抑制作用渐明显。     

Bcl-2及Bax在db/db自发性糖尿病小鼠颌下腺的表达研究

目的：观察转基因糖尿病小鼠颌下腺的形态学改变以及凋亡相关基因Bcl－2及Bax在颌下腺表达的变化。方法：取3、4、6、8、10月龄db/db糖尿病小鼠及相应月龄的db/ m小鼠（对照组）颌下泉，HE染色，光镜观察形态学改变。免疫组化染色后，计数Bcl－2及Bax阳性细胞数。结果：随着糖尿病进展，颌下腺组织萎缩。Bax阳性细胞数比各同龄对照组明显增多，且随病程延长有显著增多趋势。相反Bcl-2阳性细胞数则明显低于对照组，并随病程延长呈下降趋势，结论：抑制细胞凋亡Bcl－2表达的降低及促进细胞凋亡的Bax表达的增加提示，随着糖尿病发展，颌下腺实质细胞有凋亡增强趋势，而这种凋亡可能是组织萎缩和功能受损的重要原因之一。     

Bcl-2及Bax在大鼠腺体萎缩过程中的表达及临床意义

目的：：探讨大鼠腮腺主导管结扎后B cell lymphoma 2(Bcl-2)、Bcl-2-associated X protein(Bax)在腺体萎缩过程中的表达及临床意义。方法：对72只Wistar大鼠进行不同时间点腮腺主导管结扎建立腮腺萎缩模型;分别于术后1、3、5、7、14、21、30、60、90、150、180 d取材,采用免疫组织化学染色法检测Bcl-2、Bax在不同时间点的表达及分布情况。结果：主导管结扎后7 d多数腺泡细胞萎缩消失。Bcl-2和Bax在正常腺体组织中呈低水平表达。主导管结扎后不同时间点Bcl-2和bax在腺体中呈高表达,3 d时Bax表达达到峰值(1.99±0.10),21 d时Bcl-2的表达达到峰值(3.02±0.10),随后Bcl-2及Bax表达均降低。 Bcl-2/Bax比值在1~21 d升高,随后降低并趋于稳定。结论：腮腺主导管结扎后Bax、Bcl-2的表达与腮腺萎缩过程相关。     

增殖细胞核抗原和P53在萎缩性腮腺再生中的表达及意义

目的 从基因和蛋白水平研究增殖细胞核抗原（PCNA）和P53在萎缩性腮腺再生过程中的表达及意义。方法 将102只Wistar大鼠分为实验组和对照组,结扎实验组大鼠腮腺主导管,分别于结扎7 d（A组）、14 d（B组）后实现腮腺导管再通,并于再通后第0、3、5、7、10、14、21、28天处死大鼠获取新鲜腮腺组织标本。采用苏木精-伊红（HE）染色法观察腮腺组织形态学变化,实时荧光定量聚合酶链式反应和Western blot法检测两组腮腺组织中PCNA和P53的表达。结果 两实验组在腮腺导管结扎第7、14天,腺泡凋亡,导管细胞增殖,同组P53相对表达量高于PCNA;随着再通时间延长,腺泡细胞逐渐增多,A组在导管再通后第3天、B组在导管再通后第5天,PCNA和P53相对表达量达到峰值,与对照组比较,差异有统计学意义（P<0.01）;其后PCNA和P53相对表达量逐渐减少,A组于再通后第21天、B组于再通后第28天接近对照组。结论 腮腺导管结扎后,P53相对表达量增多,诱导腮腺腺泡凋亡;导管再通后,PCNA相对表达量逐渐增多,达峰值后减少,可促进腺泡增殖。     

热疗诱导颌面部鳞癌细胞凋亡与Bcl-2和Bax蛋白表达

目的：探讨热疗诱导颌面部鳞癌细胞凋亡与Bcl—2和Bax蛋白表达间的关系。方法：选择12例颌面部鳞状细胞癌患者，43℃ 40min微波热疗后手术切除肿瘤组织，采用TUNEL原位末端标记法检测细胞凋亡，SP免疫组化染色法检测Bcl—2和Bax蛋白表达。结果：治疗后颌面部鳞癌凋亡细胞数量明显增多，Bcl—2基因蛋白表达明显降低，Bax基因蛋白表达明显升高。结论：热疗通过下调Bcl—2基因蛋白表达、上调Bax基因蛋白表达诱导颌面部鳞癌细胞凋亡。     

αl—ACT、αl—AT和TF在腺淋巴瘤中的表达和意义

目的 检测抗糜蛋白酶(α1—antichymotrypsin／α1—ACT）、抗胰蛋白酶(α1—antitrypsin/α1—AT)及转铁蛋白(transferrin/TF)在腺淋巴瘤和腮腺正常组织中的表达，对腺淋巴瘤的上皮的组织发生学进行初步探讨。方法 应用免疫组织化学技术检测32例腺淋巴瘤和正常腮腺αl—ACT、αl—AT和TF的表达。结果 αl—ACT和αl—AT主要分布于腔细胞，TF主要分布于基底细胞；腺淋巴瘤上皮成分和腮腺腺泡细胞、闰管细胞有相似的免疫表达特征。结论 腺淋巴瘤两层上皮细胞形态结构不同，功能分化有差异；腺淋巴瘤和正常腮腺组织腺泡细胞、闰管细胞之间可能存在组织发生学联系。     

Sjgren综合征涎腺组织中凋亡相关基因免疫组化分析

目的: 探讨凋亡相关基因bcl 2、bax、fas、fasL在Sj gren综合征(Sj grensyndrome, SS)涎腺组织中的表达及其在SS病变发生、发展中的作用。方法:采用SP免疫组化法,检测 16例SS涎腺组织和 10例正常涎腺组织中Bcl 2、Bax、Fas、FasL的表达情况。结果: Bcl 2在SS腺泡和导管上皮细胞的表达较正常组显著降低,而Bax表达明显增加;Fas、FasL在SS腺泡上皮细胞中的表达均高于正常组,而在导管上皮细胞中的表达均无显著性差异。结论:Bcl 2、Bax、Fas、FasL在SS涎腺组织中的表达发生异常,使得SS涎腺上皮细胞过度凋亡,造成了腺体结构的破坏和分泌功能的丧失;而浸润性淋巴细胞的凋亡被抑制,造成淋巴细胞聚集。     

涎腺疾病

保留腮腺咬肌筋膜的腮腺切除术；P70 S6激酶在腮腺腺泡细胞癌中表达的研究；舌下腺囊肿口外型27例诊断与治疗；Bax促凋蛋白在抗DDP的人涎腺腺样囊性癌细胞中的表达；抑癌基因PTEN在涎腺癌组织中的表达     

人胚腮腺腺泡细胞的电镜观察及组织化学研究

在以往的文献中对腮腺腺泡细胞的研究较多，但大多是针对成人及动物腮腺，而对胚胎，尤其是人胚腮腺腺泡细胞的研究甚少。最近，我们在人胚颌下腺定量分析及人胚涎腺组织形态学观察与组化研究的基础上，又进行了人胚腮腺腺泡细胞的电镜观察及组织化学研究，发现其与成人腮...     

腮腺细胞程序性死亡分子5的表达及其与细胞凋亡关系的动物实验

目的 探讨大鼠腮腺主导管结扎后细胞程序性死亡分子5(programmed cell death5,PDCD5)的表达及其与腮腺细胞凋亡的关系,以期了解PDCD5在腮腺萎缩中的作用.方法 对66只Wistar大鼠建立腮腺主导管结扎动物模型,分别在结扎0、1、3、5、7、14、21、30、60、90和120 d(每个时间点6只大鼠)获取腮腺标本,免疫组化法检测腮腺细胞中PDCD5的表达,脱氧核糖核酸末端转移酶介导的原位缺口末端标记法检测腮腺细胞的凋亡.结果 PDCD5蛋白在正常的腺泡细胞和导管细胞中呈低水平表达,均匀分布于胞质.腮腺主导管结扎后,PDCD蛋白的表达水平显著上调,在结扎后3d腺泡细胞中PDCD5的吸光度值达峰值(1.261±0.048),胞质和胞核中均有表达.在结扎后1、3d,腺泡细胞中PDCD5的吸光度值均显著高于导管细胞,二者差异有统计学意义(P＜0.01).细胞凋亡检测结果显示,结扎后3d腮腺细胞凋亡指数显著增高,腺泡细胞凋亡指数[(21.750±0.119)％]显著高于导管细胞[(5.720±0.205)％],二者差异有统计学意义(P＜0.01).PDCD5蛋白的表达水平与腮腺主导管结扎诱导的细胞凋亡呈显著正相关(r=1,P=0.003).结论 大鼠腮腺主导管结扎后PDCD5的表达与腮腺萎缩过程密切相关,PDCD5在腮腺结扎后的细胞凋亡途径中可能发挥重要作用.     

Variation in periodontal referral in 2 regions in theUK

This study investigated the periodontal referral patterns of general dental practitioners (GDPs) in Northern Ireland (NI) and North West England (NWE). A questionnaire dealing with periodontal referral was sent to all 520 GDPs registered in NI and to 274 GDPs in NWE. A usable return was made by 355 (68%) in NI and 189 (70%) in NWE. The NI dentists made significantly more periodontal referrals (median 5, range 0-80) in the year preceding the survey than those in NWE (median 2, range 0-50), p<0.001. Distance was the only factor significantly related to the referral rate in both regions with those who practised more than 25 miles from a specialist referring significantly fewer patients in both regions. In NI, there was a trend towards increased periodontal referral by GDPs who had attended more postgraduate courses; however, in NWE, this was not the case. The GDPs in NWE were significantly less likely than those in NI to refer patients with medical conditions. It is concluded that there is considerable variation in periodontal referral both within and between the 2 regions studied. It is further concluded that in many cases, non-disease factors, such as the accessibility of the specialist service, have powerful effects on the decisions made by dentists and patients in these regions (NI and NWE) in relation to periodontal referral. Much of the variance in referral in North West England, as in Northern Ireland, remains unexplained.     

云南省五岁儿童乳牙龋病调查分析

目的：了解云南省５岁儿童乳牙龋齿患病情况。方法：随机抽取云南省三个城市三个农村的２１３２名５岁常住儿童,采用第二次全国口腔健康流行病学调查标准调查龋齿患病情况。结果：云南省５岁儿童乳牙患龋率为７５．７５％,龋均为４．４４,充填率仅为６．５７％,仅占构成比的４．３９％。结论：云南省５岁儿童乳牙龋齿患病率高,充填率低。儿童龋病防治应注重两个方面：１．加强对家长和教师的幼儿口腔卫生保健知识及方法的宣传。     

Mast cells in masseter muscle in experimental bruxism in man

Three human subjects performed tooth grinding for 25 min, and after 20 h biopsies of the right and left masseter muscles were examined for their contents of mast cells. In comparison with specimens from a control group of three subjects, there was an increase of degranulating mast cells in muscles that had performed bruxism.     

Experience in using moradol in osteoplastic operations in oral surgery

Osteoplastic operations on the jaws were carried out in 19 patients aged 14-64 under moradol anesthesia. The mean length of the operation was 3 hours. The authors come to a conclusion that moradol in a dose of 0.3 mg/kg b. w. ensures adequate anesthesia, with a high level of analgesia persisting in the immediate postoperative period.     

Changes in cyclooxygenase metabolities in experimental periodontitis in Macaca mulatta

The four principal metabolites of cyclooxygenase (CO) were examined during the progression of experimental periodontitis in the rhesus monkey Macaca mulatta. Thirty-two monkeys were divided in four disease-matched groups. Three groups were treated with flurbiprofen, a potent CO inhibitor, at either 0.027, 0.27 or 7.1 mg/kg/day delivered systemically by a subcutaneously-implanted osmotic mini-pump. We have previously described the findings indicating that flurbiprofen treatment significantly retarded clinical attachment loss (ALOSS), redness and radiographic bone loss (BLOSS). This investigation focuses on the changes in CO metabolites which occur during disease progression of ligature-induced periodontitis and on the dose-response relationship of flurbiprofen, as it relates to disease inhibition and the suppression of ARA metabolites within the crevicular fluid (CF). In untreated animals there was a statistically significant 3-fold increase in CF levels of prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) at 3 months, as compared to baseline, which positively correlated with increases in redness, bleeding, ALOSS and BLOSS. CF-PGE2 and TxB2 levels reached a 6-fold peak at 6 months and returned to baseline by 12 months. Flurbiprofen (Fb) prevented the 3-month rise in TxB2, but did not affect the increase in PGE2. At 6 months, Fb administration caused a dose-dependent inhibition of both PGE2 and TxB2. Probit analysis of the dose-response data revealed that the concentration of Fb which caused a 50% inhibition of CF-TxB2 level (the IC50 value for TxB2 synthesis) was approximately two logs lower than the IC50 value for PGE2 synthesis, i.e. TxA2-IC50 = 0.013 vs. PGE2-IC50 = 1.35 mg flurbiprofen/kg/d. The slopes of the PGE2 and TxB2 inhibition curves were identical, consistent with a similar mechanism or singular enzyme for the site of action of Fb inhibition of CO activity. However, the kinetics and sensitivity of Fb inhibition were significantly different for the CO activity responsible for TxB2 and PGE2 synthesis, perhaps due to different compartmentalization of CO within different cell types.