Changes in serum tumor necrosis factor (TNF-alpha) with kami-shoyo-san administration in depressed climacteric patients

An herbal medicine (kampo) is widely used to prevent or treat climacteric symptoms. In order to investigate the potential involvement of tumor necrosis factor (TNF)-alpha in susceptibility to mood disorder in climacteric women and to clarify the relationship between immune function and the efficacy of herbal medicine, we compared serum TNF-alpha levels in two treated groups, with and without concurrent use of herbal medicine. This study included 113 consecutive depressed menopausal patients who visited the gynecological and psychosomatic medicine outpatient clinic of the Osaka Medical College Hospital in Japan. Fifty-eight patients were administered kami-shoyo-san according to the definition of above sho. In contrast, 55 patients who were different in sho of kami-shoyo-san were administered antidepressants. Hamilton Rating Scale for depression (HAM-D) scores were determined at baseline and 12 weeks after starting treatment (endpoint). TNF-alpha concentrations were analyzed before and after 12 weeks of treatment. Kami-shoyo-san significantly increased plasma concentrations of TNF-alpha after 12 weeks of treatment, to 17.22 +/- 6.13 pg/ml from a baseline level of 14.16 +/- 6.27 pg/ml (p = 0.048). The percent change in plasma concentration of TNF-alpha differed significantly between the kami-shoyo-san therapy group and the antidepressant therapy group at 4 weeks (12.0 +/- 7.8% and -1.22 +/- 0.25%, respectively, p < 0.01), 8 weeks (19.7 +/- 3.4% and -2.45 +/- 0.86%, respectively, p < 0.01), and 12 weeks (21.3 +/- 5.4% and -6.81 +/- 2.2%, respectively, p < 0.001). We found in this study that kami-shoyo-san, an herbal medicine, increased plasma TNF-alpha levels in depressed menopausal patients. Cytokines may play various roles in mood and emotional status via the central nervous system and may be regulated by herbal medicines, although the interactions are very complex.     

Clinical efficacy of EH0202, a Kampo formula, on the health of middle-aged women

To investigate the clinical effects of EH0202, a Japanese herbal supplement, on the menopausal symptoms and physical status of peri- and post-menopausal women. Thirty-two post-menopausal women (53.0+/-5.1 years) presented with menopausal complaints were enrolled in the clinical study. Patients were administered an herbal supplement, EH0202 (6 g/day for 6 months), and were assessed for improvement of their overall symptoms using the Greene Climacteric Scale and the Visual Analog Scale (VAS). Blood pressure, skin surface blood flow and plasma lipid profiles were measured before and 1,3 and 6 months after EH0202 administration. There was a significant decrease in the climacteric scale score (P < 0.001) and VAS (P < 0.0001) after 6 months of EH0202 treatment. There was a significant decrease in systolic (P < 0.001) and diastolic (P < 0.05) blood pressure, and a significant decrease in facial skin surface blood flow (P < 0.05) after 3 months of EH0202 administration. We observed a significant decrease in plasma TG and LDL-cholesterol concentrations after 3 months of EH0202 administration (P < 0.05). EH0202 (MACH) reduced blood pressure, excess facial skin blood flow (flushing) and abnormal lipid levels, as well as clinically improved menopausal symptoms in post-menopausal women. In post-menopausal women, this product appears to help maintain normal biological function and improves quality of life.     

苦碟子注射液治疗中青年急性脑梗死的临床疗效及对白细胞介素-6、C反应蛋白的影响

目的观察苦碟子注射液治疗中青年急性脑梗死的临床疗效及对血浆白细胞介素-6（IL-6）、C反应蛋白（CRP）水平的影响。方法将81例中青年急性脑梗死患者随机分为2组,治疗组43例予苦碟子注射液治疗,对照组38例予曲克芦丁注射液治疗。2组均连续治疗14 d后观察临床疗效,并测定2组治疗前后血浆IL-6及CRP水平。结果 2组治疗后IL-6、CRP均较本组治疗前降低（P〈0.05,P〈0.01）,且治疗组治疗后IL-6、CRP均低于对照组（P〈0.05）。治疗组总有效率86.04%,对照组总有效率73.68%,2组总有效率比较差异有统计学意义（P〈0.05）,治疗组疗效优于对照组。结论苦碟子注射液可通过降低IL-6及CRP水平来发挥保护脑组织作用,提高临床疗效。     

冠心病心衰患者白细胞介素-6浓度的变化

目的 :观察老年冠心病轻中度心衰患者血浆白细胞介素 -6(interleukin -6,IL -6)浓度的变化。方法 :选择 34例老年冠心病轻中度心衰患者和 30例健康老年人 ,测定其血浆IL -6浓度。结果 :心衰组血浆IL -6浓度明显高于对照组 (P <0 .0 1)。心衰组血浆IL -6浓度与左室射血分数(LVEF)呈负相关 (r =-0 .5 2 ,P <0 .0 1)。结论 :老年冠心病轻中度心衰患者血浆IL -6浓度明显升高 ,IL -6在心衰的发生发展中起重要作用     

胃癌术后早期小肠内滴注健脾通里中药对血清IL-2、sIL-2R和IL-12的影响

目的探讨小肠内滴注健脾通里中药对胃癌术后早期细胞因子白细胞介素-2（IL-2）、可溶性白介素-2受体（sIL-2R）和白细胞介素-12（IL-12）调控及其临床意义。方法60例将要手术的胃癌患者随机分为研究和对照两组。研究组（30例）术后第1天小肠内滴注健脾通里中药;对照组（30例）术后第1天小肠内滴注生理盐水;疗程均为7天。观察手术前后IL-2、sIL-2R和IL-12变化。结果与术前比较,两组IL-2、IL-12升高,sIL-2R降低,研究组差异有显著性（P〈0．05）;对照组上述指标虽有所改善,但差异无显著性（P〉0．05）;研究组与对照组比较,IL-2升高和sIL-2R降低,差异有显著性（P〈0．05）。结论胃癌术后早期肠内滴注健脾通里中药能够提高机体免疫功能;这对防治术后早期严重并发症的发生,改善预后、提高临床生存率都具有重要意义。     

蝉贝咳喘平治疗支气管哮喘的临床与实验研究

目的：观察蝉贝咳喘平治疗支气管哮喘的临床疗效，并探讨其机制。方法：将９６例哮喘患者随机分为蝉贝咳喘平治疗组（治疗组）和桂龙咳喘宁对照组（对照组）。观察治疗前后哮喘症状及体征、肺功能、Ｔ淋巴细胞功能亚群、可溶性白介素－２受体（ｓＩＬ－２Ｒ）、血浆血栓素Ｂ２（ＴＸＢ２）及６－酮－前列腺素Ｆ１α（６－ｋｅｔｏ－ＰＧＦ１α）等的变化。实验研究观察了对豚鼠哮喘的影响。结果：（１）治疗组临床控显率和有效率均高     

类风湿性关节炎两种不同证型与三种可溶性细胞因子受体？…

目的：探讨痹证中类风湿性关节炎（ＲＡ）的两种证型（寒湿阻络证和湿热阻络证）与３种可溶性细胞因子受体,即可溶性白细胞介素２、６受体,可溶性肿瘤坏死因子受体之间的关系。方法：用ＥＬＩＳＡ法测定两证型患者血清中３种ｓＣＫＲ。结果：ＲＡ患者中３种ｓＣＫＲ均显著高于健康人,经治疗后均显著下降;寒湿阻络证ｓＩＬ－２Ｒ显著高于湿热阻络证,而ｓＴＮＦＲ１显著你芋湿热阻络证,ｓＩＬ－６Ｒ则两证型未见明显差异。结论：     

从免疫抑制和循环肿瘤细胞探讨肺癌核心病机“正虚伏毒”的生物学基础

目的：筛选非小细胞肺癌（Non-small cell lung cancer,NSCLC）患者转移高危因素。方法：收集70例I-IV期NSCLC患者的基本临床资料,并检测外周血中免疫细胞因子、循环肿瘤细胞的表达水平,并随访无进 展生存期;分析影响无进展生存期的独立因素。结果：单因素分析显示：CTC计数与IL-6水平呈正相关（rs = 0.224,P < 0.05）;IL-6水平（rs = -0.267,P < 0.05）、sIL-2R水平（rs = -0.252,P < 0.05）与PFS呈负相关;临床分期 （rs = -0.304,P < 0.05）与PFS呈负相关;CTC计数（rs = -0.278,P < 0.05）与PFS呈负相关。根据临床检测指标的 正常值范围,分成不同的亚组,结果显示IIIb-IV期患者PFS为11.00个月,与I期-IIIa期比较具有统计学意义 （P < 0.05）;IL-6高表达组患者PFS为10.00个月,与低表达组和正常组比较差异有统计学意义（P < 0.05）;sIL- 2R正常表达组PFS为25.00个月,sIL-2R高表达组患者PFS为8.00个月,三组之间比较具有统计学意义（P < 0.05）。结论：非小细胞肺癌患者外周血中CTC计数、IL-6、sIL-2R可能是NSCLC患者预后的独立影响因子;且 IL-6的表达水平与外周血中CTC计数具有正相关;初步显示外周血中的免疫抑制因素是CTC高表达的原因之 一,而干预CTC将成为阻断转移的重要途径。     

清肠化湿方对实验性结肠炎小鼠IL-6反式信号转导的影响

目的：观察清肠化湿方对实验性结肠炎小鼠白介素6（IL-6）反式信号转导trans-signaling的影响,初步探讨该方治疗溃疡性结肠炎（UC）作用机制是否与调控IL-6 trans-signaling有关。方法： TNBS法制备小鼠结肠炎模型,药物干预后,ELISA法检测可溶性白细胞介素6受体（sIL-6R）含量,实时荧光定量PCR法检测IL-6、糖蛋白130（gp130）mRNA 相对表达水平,Western Blot法观察结肠黏膜IL-6、gp130蛋白的表达情况。结果：模型组小鼠sIL-6含量、IL-6及gp130 mRNA和蛋白表达水平较正常小鼠明显增高。清肠化湿方可以降低实验性结肠炎小鼠结肠sIL-6水平（P<0.01）,降低IL-6及gp130mRNA 和蛋白表达水平（P< 0.01）。 结论：清肠化湿方对小鼠实验性结肠炎发挥良好抗炎效应,可能与调控IL-6 trans-signaling有关。     

Effects of electro-acupuncture on serum cytokine level and peripheral blood lymphocyte subpopulation at immune-related and non-immune-related points

The purpose of this study is to find out whether electro-acupuncture (EA) at acupoint LI.10 (Shou San Li), which has not been considered as an immune-related point, has a similar immune effect as LI.11 (Qu Chi). We recruited 24 male medical students for this study. They were randomized into 3 groups: control group (without acupuncture during the study period, n=7), LI.11 group (EA at LI.11 acupoint, n=9) and LI.10 group (EA at LI.10 acupoint, n=8). At the first day (Day 1), blood was taken from all subjects. But EA was applied to the LI.11 and LI.10 group immediately after blood drawing. On the second day (Day 2), EA was applied to the LI.11 and LI.10 group right before blood sample was taken. At the fourteenth day (Day 14), blood sample was taken for all subjects. The parameters of EA were 4 sec alternating intervals of 2 and 15 HZ (dense and disperse) and 1-4 mA in intensity for 20 min. The waveform of electrical impulse was bi-directional and pulse duration of 50 microsec. Blood analysis included flow cytometry for lymphocyte subpopulations (CD4 and CD8 positive cell percentages) and ELISA for cytokines [soluble interleukin-2 receptor (sIL-2R), interferon (INF)-gamma, IL-4, IL-6, and IL-10]. We treated the result of Day 1 as a baseline value. After EA at acupoint LI.11, CD4 positive cell percentage at Day 14 showed significant decrease compared to the baseline value (p = 0.011). CD8 positive cell percentage also decreased at Day 2 (p = 0.038) and Day 14 (p= 0.015). In the LI.10 group, CD4 and CD8 levels at Day 14 were decreased significantly (CD4, p = 0.012; CD8, p = 0.017). Results of all cytokines concentrations of the LI.11 group did not change during the study period. But the serum level of sIL-2R of the LI.10 group at Day 14 was higher than the baseline value (p = 0.018). The serum level of IL-6 at Day 14 was lower than the baseline value (p = 0.028). The serum level of IL-4 at Day 2 was lower than the baseline value (p = 0.025). Our results showed that LI.11 and LI.10 acupoints cause a similar decrease of CD4 and CD8 populations. Increase of serum sIL-2R and decrease of serum IL-4 and IL-6 were observed when EA was applied at LI.10 acupoint, but not at LI.11 acupoint. In this study, we can understand that EA may modulate the peripheral blood lymphocyte subpopulations and serum cytokine levels of the immune system.     

归脾丸加减对围绝经期功血患者免疫功能的影响

目的:探究归脾丸加减对围绝经期功血患者免疫功能、白细胞介素2(IL-2)、可溶性白细胞介素-2受体(sIL-2R)的影响。方法:选取2019年3月至2018年6月山东省济南市中西医结合医院收治的符合纳入条件的围绝经期功血患者118例作为研究对象,按照就诊顺序编号随机分为对照组和观察组,每组59例。对照组常规西医治疗,观察组加用归脾丸加减治疗,均治疗3个月。观察2组治疗前、完成治疗后T淋巴细胞亚群、IL-2、sIL-2R、凝血功能、子宫内膜厚度、激素水平、症状积分变化;完成治疗后统计治疗过程中的不良反应。结果:1)完成治疗后,2组CD3+、CD4+、CD4+/CD8+、IL-2较治疗前均显著升高,CD8+、sIL-2R则显著下降(P<0.05);完成治疗后观察组CD8+、sIL-2R显著低于于对照组,余均高于对照组(P<0.05)。2)完成治疗后2组凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、子宫内膜厚度较治疗前均显著下降(P<0.05),完成治疗后观察组以上指标均显著低于对照组(P<0.05)。3)完成治疗后2组黄体生成素(LH)、促排卵生成素(FSH)、雌二醇(E2)、孕酮(P)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)较治疗前均显著下降(P<0.05),完成治疗后观察组以上指标均显著低于对照组(P<0.05)。4)完成治疗后2组月经周期、阴道出血量、阴道出血天数、神疲乏力积分较治疗前均显著下降(P<0.05),完成治疗后观察组以上指标均显著低于对照组(P<0.05)。5)完成治疗后观察组胃肠道反应、体质量增加、肝功能异常、乳房胀痛发生率均显著低于对照组,2组比较差异有统计学意义(P<0.05)。结论:归脾丸加减能提高围绝经期功血患者免疫功能,抑制IL-2、sIL-2R,改善凝血功能,从而改善症状,提高疗效。     

依达拉奉联合苦碟子对急性脑梗死C反应蛋白和IL-6的影响

目的探讨依达拉奉联合苦碟子对急性脑梗死患者血浆C反应蛋白（CRP）和白细胞介素-6（IL-6）水平的影响。方法92例急性脑梗死随机分为依达拉奉注射液联合苦碟子注射液治疗组（治疗组）和苦碟子注射液治疗组（对照组）,治疗前和治疗2个疗程后测定血浆CRP和IL-6水平。结果两组患者治疗后,对照组和治疗组的CRP较治疗前分别地降低18.8%、46.7%,IL-6浓度较治疗前分别降低12.7%和9.6%,经统计学处理均有统计学意义（P〈0.05或P〈0.01）。结论依达拉奉联合应用苦碟子可通过降低CRP及IL-6水平来发挥保护脑组织作用。     

痛泻要方对溃疡性结肠炎大鼠下丘脑中IL-6，IL-6R表达的影响

目的:观察痛泻要方对溃疡性结肠炎(ulcerative colitis,UC)大鼠下丘脑中白细胞介素-6(IL-6)和白细胞介素-6受体(IL-6R)基因和蛋白表达的影响。方法:将SPF级Wistar大鼠运用复合方法进行溃疡性结肠炎造模后,随机分为5组:模型组,痛泻要方低、中、高剂量(2.75,5.5,11 g·kg~(-1))组,美沙拉嗪(5-ASA)组。药物处理大鼠21 d后麻醉处死,肉眼观察结肠大体形态损伤并进行评分。分离各组大鼠血清,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中IL-6含量,分离大鼠下丘脑组织并提取总RNA后,实时荧光定量PCR(Real-time polymerase chain reaction,Real-time PCR)法检测IL-6和IL-6R基因表达,免疫组织化学染色法检测下丘脑组织IL-6和IL-6R的分布及蛋白表达量。结果:ELISA结果显示,与空白组比较,模型组大鼠血清中IL-6含量明显升高,经过药物处理后,药物组血清中IL-6含量均有所下降,其中以痛泻要方高剂量组效果最为明显(P0.05)。Real-time PCR结果表明,模型组大鼠下丘脑组织中IL-6和IL-6R基因表达量明显高于空白组(P0.05),经药物治疗后,药物组IL-6和IL-6R基因表达均有所降低,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P0.05)。免疫组织化学染色结果表明,与空白组比较,溃疡性结肠炎大鼠IL-6和IL-6R蛋白表达量明显增加(P0.05),药物处理可以降低大鼠下丘脑IL-6和IL-6R蛋白表达量,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P0.05)。结论:痛泻要方对2,4,6三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)/乙醇法UC大鼠下丘脑组织中IL-6和IL-6R基因和蛋白的表达具有明显抑制作用,提示痛泻要方治疗溃疡性结肠炎的机制可能与抑制下丘脑中IL-6/IL-6R信号通路的异常活化有关。     

大黄联合肠内营养对胃癌围手术期患者外周血C反应蛋白和白细胞介素6水平的影响

目的 观察应用中药大黄和肠内营养对胃癌围手术期患者外周血C反应蛋白（C- reactive protein，CRP）、白细胞介素6（interleukin 6， IL-6）水平的影响。方法 将56例胃癌围手术期患者随机分为对照组20例，肠内营养组21例，大黄加肠内营养组15例。对照组术后常规补液的同时实施肠外营养支持；肠内营养组术后常规补液的同时术后42 h开始给予肠内营养液能全力鼻饲管滴入，一直持续到术后第8天；大黄加肠内营养组患者术前1天应用大黄液100 mL(10 g)，术后18 h、42 h各用大黄液50 mL(5g)，同时给予肠内营养液能全力。所有研究对象分别于手术前1天、手术后第1、3、7天检测血清CRP、IL-6。结果 3组患者术后均有急性炎症反应的发生，但大黄加肠内营养组术后第7天血浆CRP水平〔（12.67±4.28）mg/L〕和IL-6水平〔（18.17±11.10）mg/L〕均明显下降，与对照组〔（19.72±9.73）mg/L、（20.44±6.32）mg/L〕及肠内营养组〔（21.27±10.46）mg/L、（20.29±7.79）mg/L〕比较，差异均有统计学意义（P＜0.05）；大黄加肠内营养组肠鸣音的恢复较非大黄组提前。结论 胃癌患者围手术期应用大黄可以有效降低外周血CRP 、IL-6水平，缓解手术创伤引起的急性炎症反应,促进术后胃肠道功能恢复, 有利于实施术后肠内营养支持。     

补肾健脾方改善帕金森病患者非运动症状和血清细胞因子水平临床研究

目的:研究补肾健脾方治疗帕金森病患者疗效及对非运动症状和血清细胞因子水平的影响。方法:将82例帕金森病患者随机分为多巴丝肼组和治疗组,每组41例。多巴丝肼组采用多巴丝肼进行治疗,治疗组在多巴丝肼组的基础上采用补肾健脾方进行治疗。观察两组血清细胞因子水平、非运动症状评价量表指标、症状、抑郁情况、睡眠质量及生活质量。比较两组疗效、不良反应发生情况。结果:治疗后,治疗组总有效率高于多巴丝肼组(P<0.05)。与治疗前比较,两组治疗后血清白介素-6(IL-6)、可溶性白介素-2受体(sIL-2R)、胱抑素C(CysC)水平及非运动症状评价量表各项评分、帕金森病综合评分量表(UPDRS)、汉密尔顿抑郁量表(HAMD)、39项帕金森病生活质量量表(PDQ-39)评分降低,治疗组以上指标均低于多巴丝肼组(P<0.05);治疗后两组血清脂联素(APN)水平及帕金森病睡眠量表(PDSS)评分升高,治疗组高于多巴丝肼组(P<0.05)。两组治疗期间总不良反应发生率比较差异无统计学意义(P>0.05)。结论:补肾健脾方可改善帕金森病患者非运动症状、睡眠质量、生活质量,降低机体炎症反应...     

RNA干扰抑制脊髓源星形胶质细胞sIL-6R基因表达的实验研究

目的:靶向白细胞介素6受体(sIL-6R)基因构建具有高干扰效率的RNA干扰载体,观察其对脊髓源星形胶质细胞sIL-6R基因表达抑制效果。方法:靶向sIL-6R基因设计3条短发夹样RNA(short hairpin RNA,shRNA)的寡核苷酸片段,构建sIL-6R siRNA重组质粒真核表达载体,转染脊髓源星形胶质细胞,筛选出对sIL-6R基因干扰效率最高的sIL-6R siRNA,用免疫组化染色、RT-PCR及Western blot技术观察其对脊髓源星形胶质细胞sIL-6R基因表达抑制效果。结果:成功构建sIL-6R siRNA重组质粒真核表达载体,免疫组化染色、RT-PCR及Western blot检测结果证实sIL-6R siRNA能显著下调sIL-6R的表达。结论:sIL-6Rs iRNA重组质粒真核表达载体对脊髓损伤后sIL-6R表达有明显的抑制作用,为后续多靶点RNA干扰技术在脊髓损伤修复中的应用奠定了前期基础。     

薯蓣皂苷对动脉粥样硬化大鼠白介素-6的影响

目的 探讨薯蓣皂苷对动脉粥样硬化模型大鼠白介素6 (interleukin-6,IL-6)的影响.方法 采用随机数字表法将60只雄性SD大鼠随机分成:假手术组、模型组、阿司匹林组、薯蓣皂苷低剂量组及高剂量组.饲养7d后,除假手术组,余大鼠于左侧颈总动脉行主动脉内膜球囊拉伤术.3个月后,行颈总动脉HE染色,采用图象分析仪...     

Comparing the effects of estrogen and an herbal medicine on peripheral blood flow in post-menopausal women with hot flashes: hormone replacement therapy and gui-zhi-fu-ling-wan, a Kampo medicine

We investigated the association between blood flow in the extremities and hot flashes, and compared change in blood flow following hormone replacement therapy (HRT) and Gui-zhi-fu-ling-wan (Keishi-bukuryo-gan), a herbal therapy in post-menopausal women with hot flashes. Three hundred and fifty-two post-menopausal women aged 46-58 years (mean: 53.4 +/- 3.6 years) with climacteric complaints participated in the study. One hundred and thirty-one patients with hot flashes were treated with HRT (64 cases) or herbal therapy (67 cases). Blood flow was measured with laser doppler fluxmetry under the jaw, in the middle finger and in the third toe. Post-menopausal women with hot flashes (129 cases) showed significantly higher blood flow under the jaw (13.6 +/- 4.13) than women without hot flashes (166 cases) (5.48 +/- 0.84) (p < 0.0001). Blood flow at this site decreased significantly with either therapy (p < 0.0001). On the other hand, the administration of Gui-zhi-fu-lingwan significantly increased (p = 0.002) the blood flow in the lower extremities, whereas HRT decreased the blood flow. Thus, we have demonstrated that Gui-zhi-fu-ling-wan did not affect the activity of vasodilator neuropeptides on sensory neurons of systemic peripheral vessels uniformly. Therefore, Gui-zhi-fu-ling-wan, rather than HRT, is suggested as an appropriate therapy for treatment of hot flashes in the face and upper body with concomitant coldness in the lower body, which is one of the symptoms of menopause.     

五色培元固本膏对维持性血液透析患者细胞免疫功能的影响

目的:探讨五色培元固本膏对维持性血液透析(MHD)患者细胞免疫功能的影响。方法:90例MHD患者随机分为对照组和治疗组,两组血透方法相同。对照组给予一般治疗及血透治疗,治疗组在对照组基础上口服五色培元固本膏,连续治疗3个月; 20例健康志愿者作为正常组,三组观察时间均为3个月。治疗前后用流式细胞技术检测各组T细胞亚群表达水平,酶联免疫吸附法检测各组白介素-2(IL-2)和可溶性白介素-2受体(sIL-2R)蛋白表达水平。结果:治疗前与正常组比较,对照组与治疗组CD₄⁺、CD₄⁺/CD₈⁺和IL-2表达水平均明显下降,CD₈⁺和sIL-2表达水平均上升,差异有统计学意义(P<0.05)。治疗后,与对照组相比较,治疗组CD₄⁺、CD₄⁺/CD₈⁺、IL-2表达水平明显升高,CD₈⁺和sIL-2表达水平均降低,差异有统计学意义(P<0.05)。治疗组相较正常组CD₄⁺T淋巴细胞、CD₄⁺/CD₈⁺、IL-2表达水平,差异无统计学意义(P>0.05),CD₈⁺、sIL-2表达水平升高。结论:五色培元固本膏调节T淋巴细胞和IL-2表达改善MHD的细胞免疫功能。     

粉防己碱对急性出血坏死性胰腺炎大鼠炎性细胞因子含量的影响

 目的通过测定血清及胰腺组织炎性细胞因子含量,研究粉防己碱治疗大鼠急性出血坏死性胰腺炎的机制。方法胆胰管逆行加压注射4.5%牛磺胆酸钠制备急性出血坏死性胰腺炎(AHNP)动物模型;ELISA法测炎性细胞因子白细胞介素1(IL-1)、白细胞介素6(IL-6)、肿瘤坏死因子(TNF-α)含量。结果6h后AHNP大鼠血清及胰腺组织IL-1,IL-6,TNF-α明显升高。60及30mg·kg^-1粉防己碱均可降低血清及胰腺组织内IL-1,IL-6及TNF-α的含量。结论粉防己碱对大鼠急性出血坏死性胰腺炎的治疗作用与降低血清及胰腺组织IL-1,IL-6,TNF-α含量有关。