川黄颗粒的薄层鉴别及丹参素钠的含量测定

目的 建立川黄颗粒鉴别和含量测定的方法.方法 采用TLC法和HPLC法.结果 TLC鉴别川黄颗粒中的何首乌、黄芪、党参、枸杞子等药材;HPLC测定丹参素钠的含量,丹参素钠在0.26～1.84 mg的范围内,线性关系良好(r=0.9996).平均回收率为99.8%,RSD=1.4%.结论 鉴别重复性好、专属性强,含量测定方法简便、准确.     

肾消颗粒质量标准研究

目的:建立肾消颗粒的质量标准。方法:采用TLC法鉴别肾消颗粒中黄芪、淫羊藿,采用高效液相色谱法测定制剂中丹参素钠、原儿茶醛和淫羊藿苷含量。结果:TLC鉴别色谱特征斑点清晰;高效液相色谱法测得丹参素钠在0.15～2.40μg范围内线性良好(r=0.999 9),平均回收率为100.3%,RSD为2.23%;原儿茶醛在0.012～0.192μg范围内线性良好(r=0.999 9),平均回收率为99.0%,RSD为2.23%;淫羊藿苷在0.058～0.580μg范围内线性良好(r=0.999 9),平均回收率为96.1%,RSD为1.89%。结论:方法专属性强、简便、重复性好,可用于肾消颗粒的质量控制。     

健脾降脂颗粒质量标准提升研究

目的 提升健脾降脂颗粒的质量控制标准。方法 采用高效液相色谱法对党参，以及薄层色谱法对远志、丹参进行定性鉴别；采用高效液相色谱法测定丹参素钠的含量，色谱柱为DiamonsilTMC18柱（250 mm×4.6 mm,5μm），流动相为甲醇-1%冰醋酸溶液（6∶94,V/V），流速为1.0 mL/min，检测波长为280 nm，进样量为20μL。结果 色谱中能明显检出远志、丹参、党参，且阴性对照无干扰，专属性强。丹参素钠质量浓度在0.542～54.200μg/mL范围内与峰面积线性关系良好（R2=0.999 9,n=6）；精密度、稳定性、重复性试验结果的RSD均小于2.0%(n=6)；平均加样回收率为101.86%,RSD为1.68%(n=6)。结论 所建立的方法简便、准确、专属性强，可为健脾降脂颗粒质量标准的提升提供参考。     

白斑颗粒的薄层色谱鉴别

目的:建立白斑颗粒的质量控制标准.方法:采用薄层色谱法对白斑颗粒中何首乌、丹参、桃仁、赤芍进行定性鉴别.结果:在薄层层析色谱中均能检出何首乌、丹参、桃仁和赤芍.结论:方法简便、专属性强,可用于该制剂的质量控制.     

滋阴活血利咽颗粒超声波提取工艺研究

目的:首次在滋阴活血利咽颗粒剂制备工艺中采用超声波提取方法,并优化了超声波提取工艺条件.方法:采用正交试验方法,考察颗粒粒度、加水量、提取次数、提取时间对超声提取得率的影响.结果:颗粒柱度、加水量、提取时间等因素对提取效果的影响均没有显著性,提取次数对提取效果的影响有显著性.结论:超声提取具有能耗低,省时,提取转移率高,常温提取,生产成本低等优势.     

生发颗粒的薄层色谱鉴别

目的建立生发颗粒的质量标准。方法采用薄层色谱法对生发颗粒中何首乌、丹参、白芍进行定性鉴别。结果在TLC色谱中检出何首乌、丹参和白芍。结论所建立的方法简便、分离度好、专属性强，可用于该制剂的质量控制。     

心可舒丸中三七的薄层色谱鉴别和6种物质的含量测定

目的 完善心可舒丸的质量控制方法.方法 采用薄层色谱法鉴别三七,采用HPLC法测定丹参素钠、原儿茶醛、丹酚酸B、葛根素、木香烃内酯和去氢木香内酯的含量.结果 三七的薄层鉴别斑点清晰,分离度好;丹参素钠、原儿茶醛、丹酚酸B、葛根素、木香烃内酯和去氢木香内酯的线性关系良好,平均加样回收率分别为99.0％、100.1％、97.9％、100.8％、100.7％、100.7％ (n =9).结论 所用方法专属性强、准确度高、重复性好,可用于心可舒丸的质量控制.     

HPLC法测定红曲灵芝丹参胶囊中4种成分的含量

目的建立同时测定红曲灵芝丹参胶囊与丹参提取物中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA含量的HPLC法。方法采用Agilent TC-C18(250mm×4.6mm,5μm)色谱柱;流动相为2 mL·L~(-1)甲酸水(A)-甲醇(B),梯度洗脱;流速为0.6mL·min~(-1);柱温:30℃;检测波长:280nm;进样量:20μL。结果测定红曲灵芝丹参胶囊中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA 4种成分的含量,每1g中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA的含量分别为0.451 2,0.119,1.398和3.789mg,与丹参提取物中各成分的含量相比差异较大。结论该方法简便、准确,灵敏度高,专属性好,可用于红曲灵芝丹参胶囊与丹参提取物的质量控制。     

活血散瘀灌肠液的制备及质量控制

目的制备活血散瘀灌肠液并建立其质量控制方法。方法用丹参、大血藤、三棱、莪术等中药材制备活血散瘀灌肠液;用高效液相色谱法测定其中君药丹参的活性成分丹参素的含量。结果所得活血散瘀灌肠液为黄棕色液体;其中丹参素钠浓度在0.02～0.1mg.mL-1范围内与峰面积积分值呈良好的线性关系（r=0.9998）,平均回收率为100.0%（RSD=1.77%）;灌肠液中丹参素钠含量限度应控制在≥0.58mg.mL-1,以保证其质量。结论本品制备工艺简单、稳定,质量控制方法准确、可靠。     

丹参中水溶性成分丹参素钠的HPLC测定

目的:建立丹参中水溶性成分丹参素钠的含量测定方法.方法:采用HPLC法,色谱柱为Shimadzu ODS C18(250 mm×4.6 mm,5μm),以甲醇-水-冰醋酸(8:91:1)为流动相,流速1.0 ml·min-1,检测波长281 nm.结果:丹参素钠在5.9～29.5μg·ml-1的浓度范围内与其峰面积呈良好的线性关系(r=0.999 9),平均回收率为97.0%(RSD=0.6%,n=6).结论:该方法操作简便、结果可靠,重复性好,可用于丹参中水溶性成分丹参素钠的分析.     

“医”与“法”

2003年8月中,惠氏百宫制药有限公司邀请广东地区各大医院的专家和医院管理人员在广东东莞举行了一场别开生面的研讨会。会议包括两个议题:一是“特治星治疗严重呼吸道感染”的学术研讨;二是“医疗纠纷处理的新规则与新思路”的研讨。会上“热点”不断,台上、台下观点撞击精彩纷呈,这里我们摘录片段与大家共享。     

Laboratory Verification of "heavy" and "light" users of cannabis

For various experimental studies it is often desirable to classify categories of cannabis use. For instance, in a study of the kinetics of δ-9-tetrahydrocannabinol (THC) in man, we wanted to see if there were differences between “heavy” and “light” users of the drug. “Heavy” use was defined as once daily or more, while “light” use was defined as sporadic or occasional, no more frequent than once monthly.Previously, we had found that the 11-oic acid of THC was not only the major metabolite to appear in the urine but also that its appearance in urine was prompt and lasted for at least 72 hours after a single exposure [1]. We decided to use the presence of this material in urine as a marker for heavy and light use; one would expect to find it regularly in heavy users and rarely, if at all, among light users. All subjects entering the study declared their pattern of use and then were asked to provide a spontaneously voided urine, to be used for analysis of THC-11-oic acid.     

以"豆"祛"痘"

虽然早已过了青春期,但我那略显"婴儿肥"的脸上还是会不时地出现些小痘痘.为了消除这些可恶的家伙,我还真是没少折腾,又是吃药、吃保健品,又是用化妆品,可痘痘却大有"野火烧不尽,春风吹又生"之势,看来要消灭它们可不是件容易的事.     

Activity of "reversed" diamidines against Trypanosoma cruzi "in vitro"

Chagas' disease is an important parasitic illness caused by the flagellated protozoan Trypanosoma cruzi. The disease affects nearly 17 million individuals in endemic areas of Latin America and the current chemotherapy is quite unsatisfactory based on nitroheterocyclic agents (nifurtimox and benznidazol). The need for new compounds with different modes of action is clear. Due to the broad-spectrum antimicrobial activity of the aromatic dicationic compounds, this study focused on the activity of four such diamidines (DB811, DB889, DB786, DB702) and a closely related diguanidine (DB711) against bloodstream trypomastigotes as well as intracellular amastigotes of T. cruzi in vitro. Additional studies were also conducted to access the toxicity of the compounds against mammalian cells in vitro. Our data show that the four diamidines compounds presented early and high anti-parasitic activity (IC50 in low-micromolecular range) exhibiting trypanocidal dose-dependent effects against both trypomastigote and amastigote forms of T. cruzi 2h after drug treatment. Most of the diamidines compounds (except the DB702) exerted high anti-parasitic activity and low toxicity to the mammalian cells. Our results show the activity of reversed diamidines against T. cruzi and suggested that the compounds merit in vivo studies.     

"Sick" or "hooked": smokers' perceptions of their addiction

A postal questionnaire was completed by 233 smokers who had contacted a television company for help with stopping smoking. Subjects recorded how well each of 20 statements described their feelings about their own smoking. These responses were submitted to a principal components analysis which revealed two interpretable factors. The first ('Sick') reflected a tendency to see smoking as a sickness, and a greater concern over health consequences. The second ('Hooked') reflected a feeling of inability to give up smoking, and a resentment at other's attempts at dissuasion. Subjects with higher 'Sick' factor scores perceived greater potential benefit in their stopping (in terms of a reduction of risk of cancer). Higher 'Hooked' factor scores were given by females, older smokers, those who described themselves as more extremely addicted, and those who saw less benefit for themselves in stopping.