核转录因子kappaB在心肌缺血再灌注损伤中的作用

核转录因子是一类蛋白质 ,广泛存在于胞浆及胞质中 ,具有与某些基因上启动子区的固定核苷酸序列结合而启动基因转录的功能。核转录因子kappaB(NF κB)被认为是最重要的一种转录因子 ,是调节免疫反应、应激反应、凋亡和炎症的中心环节 ,可被多种不同刺激激活 ,参与多种基因 ,尤其是与机体防御功能有关的即早基因的表达及调控。NF κB的生物学特性与胞浆中的抑制蛋白IκB密切相关 ,IκB与NF κB的解离可导致NF κB在核内迅速转移。近年的研究发现 ,NF κB在心肌缺血预适应、缺血、缺氧、再灌注损伤以及细胞凋亡中起重要作用。以下就N…     

核转录因子Egr-1及其在肿瘤性疾病中的研究进展

大量的研究发现,细胞外信号通过细胞质中的信号转导通路,可激活核转录因子即刻早期反应因子(Egr-1)的表达合成,使其进入细胞核内,调控细胞核内的相关靶基因的转录表达,使细胞产生适应性变化。因此细胞的许多生物学功能可归因于转录因子Egr-1的表达活化。本文探讨了在细胞增殖和细胞凋亡的过程中,转录因子Egr-1的生物学功能。特别是其在肿瘤性疾病发生、发展中的作用已成为近年来研究的热点。     

核因子kappa B的上游信号传导途径

核因子kappa B是十多年前发现的一种重要的核内转录因子，它广泛存在于各种细胞中，在细胞炎症反应，免疫反应以及细胞凋亡等过程中发挥重要作用。人们对核因子kappa B的调节过程虽还不十分清楚，但目前已得到多数人认同的是，其上游激活途径的特点是不同的起始旁路汇聚于一条共同通道。在此，作者就该因子kappa B上游传导途径作一简要介绍。     

核因子-kB与恶性肿瘤

核因子-kB(NF-kB)是一种重要的转录调控因子，可与多种基因启动子部位的出位点发生特异性结合而促进转录表达，它是由Sen和Baltimore在1986年首先从成熟B淋巴细胞中抽提出的可与免疫球蛋白重链和κ轻链基因增强子序列(GGGACTTTC)特异结合的核蛋白因子，是一种调控基因表达的DNA结合因子。此后，逐步发现它是一种普遍存在的转录因子，存在于许多类型的细胞中，     

核因子-κB的活化与急性肺损伤

核因子-кB是一种重要的转录因子，它能与介导急性肺损伤的多种细胞因子基因的启动子特异性序列结合，参与炎症介质基因转录调控，在急性肺损伤发病中有重要作用。调控核因子-кB成为治疗急性肺损伤的新策略。     

核因子-kb与急性肺损伤

转录因子核因子 ｋｂ是一类能与多种基因启动子部位的ｋｂ位点发生特异性结合并促进转录的蛋白质的总称。它通过调控许多重要的细胞因子 ,粘附分子和趋化因子的基因表达 ,而参与机体的各种免疫及炎症反应。由于核因子 ｋｂ在调控多种炎症因子基因表达上起着重要作用 ,而急性肺损伤的本质是多种致病因素引起的肺组织中性粒细胞为主的炎症细胞浸润 ,以及细胞因子及粘附分子的过度表达和炎症介质的释放引起的炎症反应 ,它与急性肺损伤的关系已成为近年来研究的热点。1　ＮＦ ｋｂ的结构和功能、活化及调节、生物学效应1.1　ＮＦ ｋｂ的结构和…     

缺氧诱导因子-1与肿瘤血管生长

缺氧诱导因子-1(HIF-1)是调节缺氧反应基因的一个核转录因子，由α和β两种亚基组成，在肿瘤血管生长、转移中起重要的作用。本文就HIF-1的结构和功能与活性调节及参与信号转导作用等方面的研究进展作一综述。     

转录因子在滋养细胞浸润行为调控中的作用

在妊娠早期,滋养细胞的浸润调控对维持母胎界面的胎盘功能发挥着重要作用,该文综述转录因子如过氧化物酶体增殖物激活受体、转录因子激活蛋白-2α、碱性螺旋- 环- 螺旋蛋白家族、GCM1、核转录因子及同源异型盒基因家族等在滋养细胞浸润的调节过程中的作用机制,有利于进一步阐明滋养细胞浸润相关性疾病的发病机制。     

乙型肝炎病毒X蛋白与核转录因子NF-κB在肝癌细胞程序性死亡中的作用

目的 :研究乙型肝炎病毒X蛋白与核转录因子NF κB的关系及其在肝癌细胞程序性死亡中的作用。　方法 :用脂质体介导的基因转染法 ,将乙型肝炎病毒X基因真核表达载体pCDNA 3.1 HBX转染入人肝癌细胞系HCC- 92 0 4 ,用免疫组化SP法和免疫荧光法对细胞内源性核转录因子NF κB进行定位 ,然后用蛋白酶抑制剂ALLN作用于细胞 ,抑制核转录因子NF κB的活化 ,阿霉素诱导肝癌细胞程序性死亡 ,检测肝癌细胞程序性死亡率的变化。　结果 :未进行基因转染的人肝癌细胞系HCC 92 0 4的内源性核转录因子NF κB仅位于胞质 ,胞核中无表达 ;转染乙型肝炎病毒x基因真核表达载体pCDNA 3.1 HBX肝癌细胞的胞质和胞核同时有核转录因子NF κB的表达 ;而在经抑制剂ALLN作用的转染乙型肝炎病毒真核表达载体pCDNA 3.1 HBX的人肝癌细胞HCC 92 0 4 ,核转录因子NF κB仅定位于肝癌细胞的胞质内 ,而且细胞程序性死亡率由 8.1%增高至 5 1.8%。　结论 :乙型肝炎病毒X蛋白可激活肝癌细胞转录因子NF κB ,使其从胞质中转位于胞核 ;乙型肝炎病毒X蛋白通过活化核转录因子NF κB而抑制肝癌细胞的程序性死亡。     

核转录因子协同刺激物OCA-B作用研究进展

OCA-B,又称为BOB.1或OBF.1,为B淋巴细胞的特异性核转录因子协同刺激物,可通过与核转录因子POU家族中的Oct-1及Oct-2相互作用而激活免疫球蛋白基因,促进抗体的生成,此外OCA-B还与B细胞的活化及生发中心的形成有密切的关系。此外在T淋巴细胞中也可检测到OCA-B表达,说明OCA-B对T细胞亦可发挥调节基因转录的作用。本文主要对OCA-B的结构、分布及生物学作用的研究进展做一阐述。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.