快速精子浓度检测试剂盒的临床实验室检测评价

目的:探讨快速精子浓度检测试剂盒的准确性、灵敏性和特异性,以评价其临床应用价值。方法:应用快速精子浓度检测试剂盒和世界卫生组织推荐的显微镜计数法分别测定临床500例不育症患者的精子浓度,并进行两种方法的kappa一致性检验。结果:快速检测精子浓度检测试剂盒和显微镜计数法比较的准确性为97.6%,特异性为97.4%,敏感性为97.8%,Kappa值为0.956,两种检测方法的结果有很好的一致性。结论:运用快速精子浓度检测试剂盒能够基本满足临床需求,其标本处理简便,有良好的应用前景。     

快速精子浓度自检试剂盒性能评价

目的:评价快速精子浓度自检试剂盒的性能。方法:对7个批号的快速精子浓度自检试剂盒进行灵敏性、特异性、准确性、稳定性等指标检测。结果:7批试剂盒灵敏性为96.7%,特异性为96.1%,准确性为96.3%,稳定性>12个月。结论:快速精子浓度自检试剂盒灵敏性、特异性、稳定性均达到要求,且具有良好的准确性和重复性。     

龙胆紫染色用于分析精子存活率的方法学研究

目的:探讨龙胆紫染色区分存活精子和死亡精子可能性,以提供一种判定精子存活率的新方法,为男性精液检测和质量评价提供依据。方法:采用不同浓度的龙胆紫溶液对精液样本染色,在光学显微镜下观察染色结果,讨论区分存活精子和死亡精子的标准,并计数一定数量的总精子数,计算存活率,并将计算结果与标准方法比较。结果:经过龙胆紫溶液(0.05%,M/V)染色后在显微镜下存活精子呈现淡蓝色,而死亡精子呈现深紫色,计算获得的存活率与伊红染色法(0.5%,M/V)的结果比较差异统计学意义(t=0.862,双侧P=0.403)。结论:一定浓度的龙胆紫染色后能够清楚地区分存活精子和死亡精子,该法获得的精子存活率结果可靠,龙胆紫染色法可以作为一种新的分析精子存活率的方法。     

双环已酮草酰二腙分光光度法快速测定农村饮用水中痕量铜

目的:在国标法双乙醛草酰二腙分光光度法的基础上,建立一种可以快速测定饮水中痕量铜的方法。方法:在pH 9的条件下,水中铜离子与双环已酮草酰二腙反应生成蓝色络合物,颜色的深浅与铜离子的含量在一定浓度范围内成正比。根据颜色的深浅在605 nm下比色定量。结果:方法最低检测浓度为0.04 mg/L;对低,中,高三个浓度,每个浓度6次的精密度实验表明,其RSD%分别为1.7%,1.2%和1.3%;6次盲样测定的相对误差在0.8%~2.3%之间。结论:该方法比国标法简便,快速,可以用于水中痕量铜的测定。     

阴道炎五联检试剂盒检测245例白带样本的结果分析

目的探讨阴道炎五联检试剂盒在白带常规检测中的意义。方法阴道分泌物同时用双盲法进行阴道炎五联检试剂盒及镜检和培养法检测,镜检使用奥林巴斯相差显微镜和普通显微镜双湿片镜检确认,乳酸杆菌和细菌性阴道病(BV)以染色镜检为金标准,白细胞和滴虫以湿片镜检为标准,念珠菌先用湿片镜检观察,然后分别进行培养和革兰染色镜检确认,以培养结果为标准。结果试剂盒与金标准进行比较,结果显示,阴道炎五联检试剂盒检测乳酸杆菌、细菌性阴道病(BV)、滴虫与金标准差异均无统计学意义(P0.05),而在检测白细胞、念珠菌上与金标准差异有统计学意义(P0.05)。结论阴道炎五联检试剂盒是一种白带常规初筛较理想的手段,能与湿片镜检起到互补的作用。     

沙门菌传统检测与应用快速检测试剂盒的比较

目的比较二者所用时间,检出限,找出比较好的沙门检测方法。方法分别用传统检测方法和沙门氏菌快速检测试剂盒对几种沙门菌标准菌株进行检测,对两者结果进行比较。结果 OXOID沙门氏菌快速检测试剂盒所用时间比传统检测方法短,但其检出限较传统检测方法高。结论实际工作中为保证工作准确,节省时间,最好应用传统检测方法与快速检测试剂盒相结合。     

精子核成熟度与精子形态的关系

目的:研究精子核成熟度与精子形态关系,探讨精子形态异常机制。方法:收集55例精液样本,其中生育组19例,畸形精子症组36例。应用CASA分析精子密度、活力,伊红染色检测精子活率,联苯胺染色分析精液白细胞浓度,改良巴氏染色分析精子形态,苯胺蓝染色法评价精子核成熟度。结果:畸形精子症组苯胺蓝染色阳性率显著高于生育组(P<0.05)。头部异常、颈部异常、尾部异常、无定型、其它畸形精子组苯胺蓝染色阳性率显著高于形态正常精子组苯胺蓝染色阳性率(P<0.05)。结论:精子核成熟异常可导致精子形态异常。     

碘盐测定中DPD确定NaClO适宜浓度的方法

目的通过对碘盐测定条件的研究,建立一种能简便、快速确定NaClO适宜浓度的方法。方法利用DPD(N,N-二乙基对本二胺)与NaClO反应生成红色的原理,用不同浓度的NaClO与草酸-磷酸反应后加入DPD观察溶液的颜色,根据溶液的颜色判断NaClO的浓度是否合适。结果用相应浓度的NaClO对浓度分别为33.9μg/ml±3μg/ml、21.2μg/ml±2μg/ml、10.6μg/ml±2μg/ml的碘标准样进行测定,并对加入DPD后溶液的颜色和标准测定的结果进行比对,通过比较二者结果,发现测定结果超出规定范围的溶液为红色,测定结果在规定范围内的溶液颜色为无色,表明二者结果一致,DPD可以用来确定NaClO的适宜浓度。结论用DPD来确定NaClO的适宜浓度是一种简便、快速、可靠的方法。     

TecraTM沙门菌快速检测试剂盒的评价研究

[目的]结合中国国情考察TecraTM沙门菌快速检测试剂盒的质量并对试剂盒应用效果进行评价.[方法]用多种沙门菌标准菌株考察TecraTM沙门菌快速检测试剂盒的准确度、用加标回收测定试剂盒的检测限、用标准非沙门菌株测定其特异度、以国标法作为参考,用实际样品考察该试剂盒的灵敏度和特异度.[结果]TecraTM沙门菌快速检测试剂盒对中国常见的37个不同血清型别的沙门菌检测的准确度为100%;检测限达到1 cfu/25 g样品;对10种非沙门菌的检测中,其特异度为100%,并呈现良好的可重复性;对370份实样检测显示.[结论]该试剂盒的灵敏度为100%,特异度为99.59%.     

一种快速、可靠的检测精子形态学的染色技术

精子形态学检测在男性不育诊断中似不失为一项重要参数。形态学正常的精子百分比、精子密度、活力及精液量是基础精液分析中首当其冲的筛查指标。精子染色技术种类繁多,其中有一些,诸如Papanicolaou染色方法的效果极好。然而,多数用于精子染色的技术非常耗时,常常拖延了精液分析结果的报告。本文报道了一种新的精子染色技术,Diff-Quik法。它原是一种用于白细胞分类检测的常规快速染色法,并就其结果与标准的Papanicolaou方法相比较。     

Laboratory evaluation of a dyed food marking technique for Culex quinquefasciatus (Diptera: Culicidae)

A method of marking adult Culex quinquefasciatus by feeding the larvae commercial hog chow dyed with methylene blue, Giemsa, and crystal violet was evaluated under laboratory conditions. Of 243 mosquitoes fed the dyed food, 230 had visible marks (94.6%). The dyed food increased the egg-adult development time from 11.4 to 12.1 d. After 9 d, 82.5% of adult mosquitoes dyed as larvae could be identified, and remained detectable for up to 15 d, their maximum laboratory life.     

LACHAT QC8500流动注射测定地表水中阴离子合成洗涤剂

目的:建立流动注射分光光度法在线测定地表水中阴离子合成洗涤剂的分析方法。方法:标准和样品由自动进样器采集,在蠕动泵的推动下,与亚甲基蓝反应、氯仿萃取和膜分离后,进入流动池在650 nm处检测,由数据处理系统自动分析数据。结果:阴离子合成洗涤剂含量0.010 mg/L～1.000 mg/L范围内线性关系良好,相关系数r=0.9995,检出限为0.005 mg/L,相对标准偏差为2.3%～4.3%,回收率为90%～101%。结论:与传统的亚甲基蓝分光光度法相比,该方法具有快速、灵敏度高、检出限低的特点,大大提高了实验室自动化程度。     

Spectrophotometric Determination of Endosulfan Using Thionin and Methylene Blue as Chromogenic Reagents

A simple, selective and sensitive spectrophotometric method is proposed for the determination of widely used organochlorine pesticide endosulfan using thionin and methylene blue as chromogenic reagents. The method is based on the liberation of sulfur dioxide from endosulfan by adding acid reagent and alcoholic potassium hydroxide. The liberated sulfur dioxide is passed through potassium iodate solution and the iodine so liberated bleaches the violet color of thionin and blue color of methylene blue and is measured at 600 nm and 665 nm respectively. This decrease in absorbance is directly proportional to the endosulfan concentration. The Beer’s law is obeyed in the range of 0.4–7.0 and 0.2–9.0 μg mL⁻¹ of endosulfan using thionin and methylene blue as reagents respectively. The molar absorptivity and Sandell’s sensitivity were found to be 1.05 × 10⁵ and 5.03 × 10⁴ L mol⁻¹ cm⁻¹, 3.85 × 10⁻³ and 8.10 × 10⁻³ μg cm⁻² of endosulfan using thionin and methylene blue as reagents respectively. The method has been applied for the determination of endosulfan in water, soil and vegetables.     

Effect of diltiazem and methylene blue on human sperm motility,viability and cervical mucus penetration: potential use as vas irrigants at the time of vasectomy

The purpose of this study was to identify compounds that could potentially be useful for vas irrigation at the time of vasectomy. We studied the in vitro effects of a group of membrane-active and ion-channel blocking agents on human sperm motility, viability and cervical mucus penetration. Diltiazem, an anti-arrhythmic drug, and methylene blue, an agent commonly used in vasography, showed the most promising effects with marked reduction of sperm motility and cervical mucus penetration after incubation with sperm for a short period of 15 min. Diltiazem was more effective than methylene blue in inhibiting the motility and viability of sperm. Furthermore, unlike methylene blue, diltiazem significantly compromised sperm viability. Other compounds studied, such as lidocaine, nicardipine and Neosporin((R)), showed only partial inhibitory activity. Based on the data reported herein, both diltiazem and methylene blue appear to be suitable candidates to be developed for vas irrigation at the time of vasectomy.     

用标记SpA染色试剂与尿液协同凝集反应快速诊断伤寒病的研究

本文报告1981~1982年,对经血液、粪便细菌培养和肥达氏反应阳性诊断的192例伤寒病人,采用SpA菌膏吸收代替离心沉淀除去尿液中非特异性凝集物质法,与诊断血清标记SpA加美蓝染料的试剂做玻片法协同凝集反应,从中获得阳性185例,符合率为96.35%和清晰易辨的满意结果,并做了154例对照试验,建立了一个取材方便、操作简易、结果快速、敏感特异、适合流行病学调查和临床诊断伤寒病的新方法,并具有明显的经济效益。     

乳腺隐匿性病灶B超定位下美蓝注射染色肿物切除

目的 探讨B超发现乳腺低回声隐匿性病灶的处理方法，发现早期乳腺癌。方法 B超定位下美蓝注射染色乳腺隐匿性病灶并行切除术。分析其优势及切除率。结果 38例隐匿性病灶全部切除，发现早期乳腺癌4例。结论 B超定位下美蓝注射染色肿物是一种微创、简便、实用方法，手术切除乳腺隐匿性病灶成功率高、有助隐匿性病灶处理和早期癌发现。     

品红亚硫酸法测定居室空气中的甲醛

目的:建立居室空气中甲醛的品红亚硫酸测定法。方法:用水为吸收液采集样品,甲醛与品红亚硫酸溶液反应后,遇硫酸生成蓝色化合物的特性进行定量分析。结果:甲醛浓度在0~2 mg/ml范围内具有良好的线性关系(r=0.9999),检出限为0.010 g/ml,样品加标回收率为97.0%~99.8%。结论:该方法不需加热,特异性好,灵敏度高,易于推广。     

Fast and Considerable Adsorption of Methylene Blue Dye onto Graphene Oxide

The quite efficient adsorption of methylene blue dye from an aqueous solution by graphene oxide was studied. The favorable electrostatic attraction is the main interaction between methylene blue and graphene oxide. As graphene oxide has the special nanostructural properties and negatively charged surface, the positively charged methylene blue molecules can be easily adsorbed on it. In the aqueous solution of methylene blue at 293 K, the adsorption data could be fitted by the Langmuir equation with a maximum adsorption amount of 1.939 mg/mg and a Langmuir adsorption equilibrium constant of 18.486 mL/mg. The adsorption amount increased with the increase of the solution pH (3–11), was not affected significantly by KCl under the examined condition and the adsorption process was exothermic in nature. The fast and considerable adsorption of graphene oxide could be regarded as a potential adsorbent for cationic dye removal in wastewater treatment process.