Effect of Urena lobataleaves extract on the inhibition of nephropathy diabetic complication

OBJECTIVE To investigate the effect of Urena lobataleaves extract on the inhibition of nephropathy diabetic complication.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,kidney organ were collected and then superoxyde dismutase(SOD)kidney level,malondialdehyda(MDA)and tumor necrosis factor-alpha(TNF-α)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD kidney level about 30%,60% and 90% respectively compared to diabetic group(P<0.05),while the MDA kidney level was decreased by 30%,60% and 70%(P<0.05)respectively.The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw were also decrease TNF-αkidney level approximately 30%,40% and 60% compared to control group(P<0.05).In diabetic groups,SOD kidney level was decreased compared to normal group(P<0.05)while the MDA and TNF-αwere increased(P<0.05).CONCLUSION U.lobata leaves extract could inhibit nephropathy diabetic complication by increasing of SOD kidney level,decreasing of MDA kidney level,and TNF-α.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.     

Potency of Urena lobataleaves extract on the inhibition of vasculopathy on diabetic rats

OBJECTIVE To know the potency of Urena lobataleaves extract on the vasculopathy inhibition of diabetic rats.METHODS This study used control group post test only with male Sprague dawley rats.Diabetic rats were induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in dose of 250,500and1000mg·kg-1 bw for 4 weeks.After scarifying,blood sample were collected and then superoxyde dismutase(SOD)serum level,malondialdehyda(MDA),tumor necrosis factor-alpha(TNF-α)and circulating endothelial cells(CECs)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD serum level about 40%,70% and 100%respectively compared to diabetic group(P<0.05),while the MDA serum level was decreased by 20%,40%and 50%(P<0.05)respectively.The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw also decrease TNF-αserum level approximately 40%,60% and 80% compared to control group(P<0.05),whereas the CECs level was decreased by 30%,50% and 70%(P<0.05)respectively.In diabetic groups,SOD serum level was decreased compared to normal group(P<0.05)while the MDA,TNF-αand CECs were increased(P<0.05).CONCLUSION U.lobata leaves extract could inhibit vasculopathy on diabetic rats by increasing of SOD serum level,decreasing of MDA serum level,TNF-αand CECs.This potency may be related to active substances which act as an anti-inflammatory and antioxidant in U.lobata extract.     

Potency of Urena lobataleaves extract on the inhibition of hepatic complication on diabetic rats

OBJECTIVE To investigate the potency of Urena lobata leaves extract on the inhibition of hepatic complication on diabetic rats.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,liver organ and blood were collected and then superoxyde dismutase(SOD)hepar level,malondialdehyda(MDA),tumor necrosis factor-alpha(TNF-α),serum glutamic oxaloacetic transaminase(SGOT)and serum glutamic piruvic transaminase(SGPT)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0,05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD hepar level about 90%,100% and 120%respectively compared to diabetic group(P<0.05),while the MDA hepar level was decreased by 40%,50% and 70% respectively(P<0.05),whereas the TNF-αhepar level was decreased by 30%,50% and 70%respectively(P<0.05).The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw decrease SGOT level approximately10%,30% and 50% compared to control group(P<0.05),while the SGPT level was decreased by 10%,20% and 40%respectively(P<0.05).In diabetic groups,SOD heparlevel was decreased compared to normal group(P<0.05)whereas the MDA and TNF-αwere increased(P<0.05).Meanwhile SGOT level and SGPT were increased in diabetic group(P<0.05).CONCLUSION U.lobataleaves extract could inhibit hepatic complication on diabetic rats by increasing of SOD hepar level,decreasing of MDA hepar level,TNF-α,SGOT and SGPT.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.     

Effect of Urena lobataleaves extract on the improvements of lipid profiles on diabetic rats

OBJECTIVE To investigate effect of U.lobataleaves extract on the improvement of lipid profiles on diabetic rats.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in dose of 250,500 and 1000mg·kg-1 bw for 4weeks.After scarifying,blood sample was collected and then total cholesterol(TC)serum level,triglyceride(TG),low density lippoprotein(LDL)and high density lipoprotein(HDL)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw decrease TC serum level approximately 15%,25% and 35% compared to diabetic group(P<0.05),whereas the TG was decreased by 10%,20% and 30%(P<0.05)respectively.The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw also were able to decrease LDL serum level about 30%,60% and 90%respectively compared to diabetic group(P<0.05),while the HDL serum level was increased by 40%,80% and 100%(P<0.05)respectively.In diabetic groups,HDL level serum was decreased compared to normal group(P<0.05)while the TC,TG and HDL were increased(P<0.05).CONCLUSION U.lobata leaves extract could repair lipid profiles of diabetic rats by increasing of HDL serum level,decreasing of TC serum level,TG and LDL.This potency may be related to active substances which act as an anti-cholesterolemia and antioxidant in U.lobata extract.     

Effect of Urena lobataleaves extract on glucagon like peptide-1 serum level by inhibition of dipeptidyl peptidase-Ⅳ on diabetic rats

OBJECTIVE To investigate effect of Urenalobataleaves extract on glucagon like peptide-1(GLP-1)serum level by inhibition of dipeptidyl peptidase-Ⅳ(DPP-Ⅳ)on diabetic rat. METHODS This study uses control group post test only with male spraque dawley rats.Diabetic rats was induced by High Fructose Diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with ethanolic extract of U.lobataleaves in dose of 250,500 and 1000mg·kg-1 for 4weeks.Blood sample were collected from the tail vein at 15 min after oral glucose administration and then DPP-Ⅳserum level and GLP-1were examined using a rat elisa kits of DPP-Ⅳ and GLP-1.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of U.lobataleaves extract at dose of 250,500 and 1000mg·kg-1 bw were able to prolong GLP-1 bioavaibility approximately 5,2and 2.5-fold respectively compared to diabetic group(P<0.05),while the DPP-Ⅳ serum level was decreased by 60%,50% and 40%(P<0.05),respectively.In diabetic groups,DPP-Ⅳ serum level was increased more and less 4-fold compared to normal group(P<0.05)while the GLP level were decreased by 8-fold(P<0.05).CONCLUSION U.lobataleaves extract could prolong GLP-1 bioavaibility by reducing of DPP-Ⅳserum level.This effect may be related to active compounds that act as an DPP-Ⅳinhibitor in U.lobata extract.     

Anti-hyperglycemic effect of ethanolic leaves extract,hexane and water of Urena lobata on diabetic rats induced by high fructose diets and streptozotocin

OBJECTIVE To investigate anti-hyperglycemic effect of ethanolic leaves extract,hexane and water of Urena lobata on diabetic rat induced by high fructose diets and streptozotocin.METHODS This study uses control group post test only with male spraque dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with ethanolic leaves extract,hexane and water of U.lobatain concentrations of 500mg·kg-1 bw for 4weeks.Blood sample were collected from the tail vein at 0,15,30,60 and 120min after oral glucose administration and then blood glucose level were measured using a commercially available glucometer.The integrated area under the postprandial glucose curves(AUCs)was calculated by the trapezoidal method.Insulin serum level examined by rat insulin elisa kits.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of ethanolic leaves extract,hexane and water of U.lobata were able to decrease glucose AUC about 40%,20% and 60% respectively compared to diabetic group(P<0.05),whereas the insulin level was increased by 2,2and 4-fold(P<0.05),respectively.In diabetic groups,glucose AUC was increased approximately 70% compared to normal group(P<0.05)while the insulin level were decreased by 14-fold(P<0.05).CONCLUSION U.lobataleaves extract could control the increase of blood glucose level after glucose administration by improve of insulin secretion.This effect may be related to active compounds that act as an anti-hyperglycemic and antioxidant in U.lobataleaves extract.     

Effect of Cymbopogon citratus Stapf extract on lipid profile and antioxidant status in streptozotocin-induced diabetic rats

OBJECTIVE To investigate the effects of Cymbopogon citratus Stapf water extract on lipid profile and antioxidant status in streptozotocin-induced diabetic rats.METHODS Diabetes was induced by injection of streptozotocin(STZ,50mg·kg-1)intraperitoneally.Diabetic rats were divided into 5groups,consisting of 6rats.GroupⅠ,reserved as diabetic control,was administered distilled water and groupⅡ,reserved as positive control,was administered glibenclamide(10mg·kg-1·d-1)throughout the duration of the experiment.Those in groupⅢ,ⅣandⅤ were administered 250,500 and 1000mg·kg-1·d-1 of the extract,respectively for 28 d.RESULTS Treatment with 500 and 1000mg·kg-1·d-1 of the extract resulted in reduction of serum AST,ALT,serum cholesterol,triglycerides and LDL,whereas HDL was found to be increased compared with diabetic control rats(P<0.05).Moreover,increased serum activities of superoxide dismutases and catalase were found in diabetic rats treated with the extract whereas serum thiobarbituric acid reactive substance was decreased,in comparison with diabetic control rat(P<0.05).CONCLUSION Cymbopogon citratus Stapf water extract provides a benefit effect on serum lipid and antioxidant effect in diabetic rats.Thus,the extract may lower cardiovascular disease risk and others complications related to hyperlipidemia and oxidative stress in diabetic patients.     

乌头提取物对心衰大鼠抗氧化能力的研究

目的：探讨乌头提取物对心衰大鼠抗氧化能力,并明确乌头提取物治疗心衰的作用。方法选取90只Wistar大鼠随机分为3组,空白组30只,对照组30只及实验组30只。实验组和对照组采用腹主动脉缩窄法制作慢性心衰大鼠模型。造模成功后对照组给予注射地高辛20 mg· kg－1,连续干预28 d;实验组在对照组的基础上加用乌头提取物,灌胃0．04 mg· kg－1,干预28 d,空白组以等体积生理盐水尾静脉注射0．04 g· kg－1。干预结束后,对大鼠采用腹腔注射麻醉方法,取腹主动脉血离心后取上层血清,按试剂盒方法测定超氧化物歧化酶（ SOD）、丙二醛（ MDA）、还原型谷胱甘肽（ GSH）。结果造模结束后,实验组与对照组SOD、MDA与GSH水平明显低于空白组,差异有统计学意义（ P＜0．05）。实验组与对照组SOD、MDA与GSH水平无明显差别,（ P＞0．05）;干预7 d及28 d后,与空白组相比,实验组与对照组SOD、MDA与GSH水平明显升高,差异有统计学意义（P＜0．05）,与对照组相比,实验组SOD、MDA与GSH水平明显升高,差异有统计学意义（P＜0．05）。结论向心衰大鼠体内注射乌头提取物,对提高心衰机体的抗氧化能力、改善心功能不全指导意义,值得临床推广。     

Methanolic extract of ceplukan leaf(Physalis minima L.)attenuates ventricular fibrosis through inhibition of TNF-α in ovariectomized rats

OBJECTIVE To investigate the effects of ceplukan leaf(Physalis minima L),which contain phytoestrogen physalin and withanolides,toward ventricular TNF-αlevel and fibrosis in ovariectomized rats.METHODS Wistar rats divided into six groups(K1:normal;K2:5 weeks ovariectomy(OVX);K3:9weeks ovariectomy(OVX),K4,K5,and K5:9weeks OVX+4 weeks ceplukan leaf's methanolic extract dose 500,1500,and 2500 mg·kg-1,respectively.TNF-αlevel measured with ELISA method.Fibrosis measured as blue color percentage in Masson′s Trichrome staining.RESULTS This study showed that prolonged hypoestrogen increase ventricular fibrosis(P<0.05).Ceplukan leaf treatment also resulted in a decreased ventricular fibrosis and TNF-αlevel in dose dependent manner compared with those of without treatment group(P<0.05).Furthermore,the TNF-αlevel normalized in rat treated with 2500mg·kg-1 Physalis minima L(P<0.05).Reduction of fibrosis positively correlated with TNF-αlevel(P<0.05,r= 0.873).CONCLUSION Methanolic extract of ceplukan leaf decrease ventricular fibrosis through inhibition of ventricular TNF-αin ovariectomized rats.Duration of hypoestrogen increase ventricular fibrosis.     

熊果酸对动脉粥样硬化大鼠氧化应激和炎性反应的影响

目的 研究熊果酸(UA)对动脉粥样硬化(AS)大鼠氧化应激和炎性反应的抑制作用,并探讨其机制.方法 采用高脂饲料喂养结合腹腔注射VD3的方法建立早期AS大鼠模型,并分别灌胃给予UA(10、20、40 mg· kg-1· d-1)和辛伐他汀(SV, 1.8 mg· kg-1· d-1)进行干预,作为干预组,另设模型组和健康对照组(喂食正常饲料并腹腔注射生理盐水).采用比色法测定血清中抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)]活性和丙二醛(MDA)、活性氧簇(ROS)含量;酶联免疫法(ELISA)测定血浆中炎性细胞因子[C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)]含量;苏木精-尹红(HE)染色观察主动脉形态结构改变并进行病变分级.结果 UA(20、40 mg· kg -1· d-1)同步干预组大鼠血清中 SOD、CAT 活性较模型组显著升高[(318.3 ±29.4)、(12.3 ±2.4)U· mL-1vs(249.3 ±25.1)、(9.3 ±1.7)U· mL-1],MDA、ROS含量显著降低[(31.2 ±5.1)vs(49.6 ±7.0) mmol· L-1和(291.3 ±31.8)vs(402.9 ±35.7)U· mL-1],且UA 40 mg· kg -1· d-1干预组GSH-Px活性显著升高[(809.3 ± 58.4)vs(676.1 ±48.5)U· mL-1];UA(20、40 mg· kg-1· d-1)同步干预组大鼠血浆中CRP、TNF-α、IL-1β、IL-6含量较模型组均显著降低,且UA 40 mg· kg-1· d-1干预组IL-10含量显著升高[(37.6 ±4.9)vs(23.9 ±3.2)ng· L-1];UA同步干预组大鼠主动脉形态结构改变较模型组明显改善、病变评分显著降低,其中以UA 40 mg· kg-1· d-1干预组效果最为显著.结论UA可能通过抑制氧化应激损伤和炎性反应而对AS大鼠起到一定保护作用.     

番茄红素对非酒精性脂肪肝模型大鼠肝细胞色素P_(450)2E1及肿瘤坏死因子-α表达的影响

目的:探讨番茄红素对非酒精性脂肪肝模型大鼠肝细胞色素P4502E1(CYP2E1)及肿瘤坏死因子-α(TNF-α)表达的影响。方法:将大鼠先分为正常对照(N)组和造模组,造模组以高脂饲料喂养8周后建立脂肪肝模型,造模成功后再分为模型对照(M)组(高脂饲料),番茄红素低(L)、高剂量(H)组(普通饲料,番茄红素10、20mg·kg-1)、自然恢复(NR)组(普通饲料)连续饲养4周,每组10只。处死大鼠观察肝组织病理学变化情况,检测肝组织中CYP2E1、TNF-α、超氧化物歧化酶(SOD)、丙二醛(MDA)、游离脂肪酸(FFA)的水平。结果:与M组比较,NR组、L组、H组大鼠肝组织病理学变化有所改善,其中L组和H组改善显著;与NR组比较,L组、H组大鼠肝组织中CYP2E1、TNF-α、MDA、FFA水平显著减少(P均<0.05),SOD水平显著升高(P<0.05或P<0.01)。结论:番茄红素能显著抑制脂肪肝模型大鼠CYP2E1和TNF-α的表达,减轻脂质过氧化反应,因而具有改善脂肪肝的作用。     

柚皮苷对实验性2型糖尿病大鼠的作用研究

目的:研究柚皮苷对实验性2型糖尿病(T2DM)大鼠的药理作用.方法:通过给予大鼠高脂饲料结合小剂量链脲菌素(30 mg·kg-1)制备T2DM模型.动物分为正常对照组、模型组、阳性对照组、柚皮苷高剂量组(400 mg·kg-1)和低剂量组(200 mg·kg-1),连续给药20 d,测定空腹血糖,糖耐量,空腹胰岛素、血脂、肝脏MDA含量及SOD的活力.结果:与糖尿病模型组比较,柚皮苷两剂量组的空腹血糖,空腹胰岛素,总胆固醇,三酰甘油和肝脏MDA含量均显著降低(P<0.05);体重减少得到缓解;肝脏SOD的活力显著提高(P<0.01).结论:柚皮苷可以纠正T2DM大鼠的糖代谢和脂质代谢紊乱,减轻胰岛素抵抗,增强机体抗氧化能力,对肝脏起到保护作用.     

乌司他丁对糖尿病脓毒症大鼠急性肾损伤的保护作用

目的 探讨乌司他丁(UTI)对糖尿病脓毒症大鼠肾损害的作用及其机制.方法 采用高脂饲料喂养+腹腔小剂量注射链脲佐菌素方法制备大鼠2型糖尿病模型,取造模成功的大鼠40只随机分为4组,每组10只:糖尿病组(D组)、糖尿病假手术组(S组)、糖尿病脓毒症组(DS组)、糖尿病脓毒症UTI预处理组(U组).另随机抽取同批次大鼠10只,作C组空白对照.采用盲肠结扎穿孔术(CLP)构建脓毒症模型.U组于CLP前1 h予尾静脉注射UTI 100 kU·kg-1.S组拉出盲肠至腹外放置1 min.于CLP术后12 h,检测各组大鼠肾组织病理情况,定量尿微量白蛋白(UMA),内生肌酐清除率(Ccr),血清肿瘤坏死因子-α(TNF-α)、白细胞介素-18(IL-18)含量,血清丙二醛(MDA)、超氧化物歧化酶(SOD)水平,以及肾组织低氧诱导因子-1α(HIF-1α)的蛋白表达水平.结果 与S组相比,DS组大鼠肾脏病理损害明显,UMA、IL-18、TNF-α、MDA、HIF-1α水平增高,Ccr和SOD活性降低(均P<0.05).DS组相比,U组大鼠肾脏病理损害减轻,UMA、IL-18、TNF-α、MDA含量、HIF-1α水平降低,Ccr和SOD活性增高(均P<0.05).结论 UTI可有效改善糖尿病脓毒症所致急性肾损伤大鼠的肾脏功能,其机制可能与抑制炎性反应、降低氧化应激、改善肾脏缺氧情况有关.     

美洛昔康对慢性应激大鼠抑郁行为的影响

目的观察美洛昔康对慢性应激大鼠抑郁行为的影响。方法采用慢性温和不可预见性应激(chronic mild un-predictable stress,CUMS)方法建立大鼠抑郁模型。美洛昔康(1、3和5 mg.kg-1)和舍曲林(5 mg.kg-1)在造模完成后灌胃给药,每天1次,连续20 d。旷场实验和强迫游泳实验检测大鼠行为学变化,生化酶学方法检测大鼠海马和皮层超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量变化,酶联免疫吸附法检测大鼠海马和皮层细胞因子白介素(IL)-1β、白介素(IL)-6、肿瘤坏死因子(TNF)-α含量变化。结果与正常组比较,慢性应激大鼠在强迫游泳实验中静止不动的次数增加(P<0.01),旷场实验中水平和垂直运动减少(P<0.05),海马和皮层SOD活性及IL-6含量降低(P<0.05),MDA及IL-1β、TNF-α含量增加(P<0.05)。与模型组比较,美洛昔康能明显改善CUMS大鼠的抑郁行为,升高海马和皮层中SOD活性及IL-6含量(P<0.05)以及降低大鼠海马和皮层MDA及IL-1β、TNF-α含量(P<0.01)。结论美洛昔康对CUMS诱导的大鼠抑郁行为有明显的改善作用,其机制可能与抑制炎症反应和氧化应激有关。     

Effect of ethanolic extract of Physalis minima on lowering blood pressure in DOCA-salts hypertensive rat models

OBJECTIVE To explore the effect of ethanolic extract of Physalis minima on lowering blood pressure in the DOCA-salts hypertensive rats.METHODS To produce hypertension,male rats were induced DOCA at dose of 10mg·kg-1 ip.twice weekly and 0.9% NaCl in drinking water ad libitum for six weeks.The ethanolic extract of Physalis minima were given at dose of 500,1500 and 2500mg·kg-1,respectively for 4weeks.After completion of treatment schedule,systolic blood pressure was evaluated using animal blood pressure analyzer.The serum nitric oxide levels was measured using the ELISA methods.SOD activity and MDA level were determined spectrophotometrically.Endothelial dysfunction was determined by acetylcholine-induced vasorelaxation studies on isolated rat aortas.RESULTS The extract decreased systolic blood pressure significantly(P<0.001)in hypertensive rats.In addition,the extract also suppressed the production of MDA and restored SOD activity to normal level.There was a reduced relaxation response to acetylcholine in isolated aortic rings and serum NO level from hypertensive rats that were reversed by extract.CONCLUSION Physalis minima can lower blood pressure in hypertensive rats and its mechanism may be through the improvement of endothelial function due to the decrease of stress oxidative.     

藏红花酸对链脲佐菌素诱导的糖尿病大鼠视网膜神经上皮的保护作用

目的研究藏红花酸对链脲佐菌素(Streptozotocin,STZ)诱导的糖尿病大鼠视网膜神经上皮的保护作用及机制。方法♂SD大鼠40只,随机选取30只腹腔注入STZ(60 mg·kg^-1)将大鼠随机分为3组:糖尿病对照组、藏红花酸低剂量组(50 mg·kg^-1·d^-1,p.o.)、藏红花酸高剂量组(100 mg·kg^-1·d^-1,p.o.)。空白对照组及糖尿病组每天给予3 mL 0.5%CMC-Na。第8周末取各组大鼠眼球检测视网膜神经上皮caspase-3、蛋白激酶C(protein kinase C,PKC)表达量,实时荧光定量PCR检测大鼠视网膜神经上皮Bcl-2、TNF-α、Bax、PKC-βmRNA表达水平。结果caspase-3、TNF-α、PKC及Bax表达量DM组表达最高,DM+CRO组比DM组明显下降(P<0.05),Bcl-2表达量DM+CRO处理组较DM组明显升高(P<0.01)。结论藏红花酸对STZ诱导的糖尿病大鼠视网膜神经上皮具有保护作用,其可能机制是抑制TNF-α、Bax、caspase-3表达,提高Bcl-2的表达,并且藏红花酸可以减少视网膜神经上皮PKC蛋白和基因的表达,保护糖尿病大鼠视网膜。     

美洲大蠊提取物Ento-A对大鼠急性胃炎胃黏膜的保护作用

目的研究美洲大蠊提取物Ento-A对急性胃炎胃黏膜的保护作用及其初步作用机制。方法将大鼠分为正常对照组,模型对照组,康复新液组,西咪替丁组,美洲大蠊提取物Ento-A高、低剂量组,连续给药8 d后,采用80%乙醇与20 mg·mL-1水杨酸钠溶液等体积混合后灌胃建立胃黏膜损伤模型,以胃炎发生计分及病理组织学评分评价Ento-A对胃黏膜的保护作用;酶联免疫法检测大鼠血清中丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)、白介素-1β(IL-1β)及胃组织匀浆中表皮细胞生长因子(epidermal growth factor,EGF)的表达水平,探究Ento-A对胃黏膜的保护作用机制。结果与模型对照组比较,康复新液、西咪替丁及Ento-A能显著降低急性胃炎大鼠血清中TNF-α、MDA、IL-1β和升高SOD的表达水平(P<0.05,P<0.01),并能提高大鼠急性胃炎胃组织匀浆中EGF的表达(P<0.05,P<0.01)。结论美洲大蠊提取物Ento-A具有明显的胃黏膜损伤保护作用,其机制可能与减少炎性因子分泌,抗自由基损伤及促进组织修复有关。     

银杏叶提取物对佐剂性关节炎大鼠血清抗氧化能力及肿瘤坏死因子含量的影响

目的:考察银杏叶提取物(EGb761)对佐剂性关节炎(AA)大鼠血清抗氧化能力及肿瘤坏死因子(TNF-α)含量的影响,初步探讨银杏叶提取物治疗佐剂性关节炎的作用机制。方法:雄性SD大鼠于右后足跖皮内注射弗氏完全佐剂(FCA)0.1 mL建立大鼠佐剂性关节炎模型。致炎第12天分别应用EGb761低、中、高剂量(50,100,200 mg.kg-1)灌胃对其进行治疗,连续给药16 d;并以雷公藤多苷(40 mg.kg-1)作为阳性对照。采用足容积法测量继发侧足肿胀度。致炎第28天处死大鼠,收集血清,检测血清中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)和一氧化氮合酶(iNOS)活力、丙二醛(MDA)、一氧化氮(NO)及TNF-α含量。结果:致炎后第12天,大鼠继发性关节炎出现,给予不同剂量的EGb761(50,100,200 mg.kg-1),从致炎后第20天开始,EGb761可明显减轻继发性AA大鼠足爪的肿胀度;与模型对照组比较,EGb761各剂量组大鼠血清中MPO、iNOS活力及MDA、NO和TNF-α含量均明显降低(P<0.05或P<0.01),而SOD含量明显升高(P<0.01)。结论:EGb761对AA大鼠继发性炎症具有显著的治疗作用,其作用机制可能与提高AA大鼠血清抗氧化能力,抑制致炎因子TNF-α生成有关。     

P2 Y12受体拮抗剂对脓毒症大鼠血小板功能和急性肾损伤影响的实验研究

目的：观察血小板P2Y12受体拮抗剂氯吡格雷对脓毒症大鼠血小板功能和急性肾损伤（AKI）的影响。方法36只雄性Wistar大鼠随机分为正常对照组（NC组）、急性肾损伤组（AKI组）、氯吡格雷组（CL组）,每组12只。AKI组、CL组分别腹腔注射内毒素（LPS）10 mg·kg－1建模;CL组在LPS注射后立即给予氯吡格雷800 mg·kg－1灌胃;建模后3 h,测定各组血小板CD62P的阳性表达水平及血小板聚集率,并检测血肌酐、尿素氮、TNF-α和IL-6的水平,取肾组织HE染色进行病理组织学观察。结果 AKI组血小板CD62P的表达水平明显高于NC组（P＜0．05）,而CL组则明显低于AKI组（P＜0．05）。AKI组血小板聚集率明显高于NC组,CL组血小板聚集率较AKI组明显降低且低于NC组（P＜0．05）。AKI组、CL组大鼠血清TNF-α和IL-6、血肌酐、尿素氮水平均高于NC组（P＜0．05）,而CL组上述指标均明显低于AKI组（P＜0．05）。AKI组大鼠肾组织病理损伤显著,CL组肾损伤程度较AKI组明显减轻。结论血小板参与AKI的发病机制,血小板P2Y12受体拮抗剂氯吡格雷可减轻内毒素诱导的肾组织的损伤,改善肾功能,其机制可能与抑制脓毒症大鼠血小板的过度活化和聚集,下调炎症因子的表达有关。     

三氧化二砷治疗鸡卵蛋白诱导日本大耳白兔类风湿关节炎的初步观察

目的：探讨三氧化二砷对鸡卵蛋白诱导日本大耳白兔致类风湿关节炎模型的滑膜组织核因子NF-κB（p65）表达活性及血清中白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）水平的影响。方法48只日本大耳白兔随机分为模型组、正常对照组、三氧化二砷低剂量组（1．0 mg·kg－1·d－1）、三氧化二砷中剂量组（2．0 mg·kg－1·d－1）、三氧化二砷高剂量组（4．0 mg ·kg－1·d－1）、醋酸泼尼松龙组（10 mg·d－1）,每组8只。成功建立卵蛋白诱导关节炎模型后,分别给药2周;采用酶联免疫吸附法（ELISA）检测兔外周血中的 IL-1、TNF-α,免疫组化实验方法检测核因子（NF-κB）（p65）在各组动物模型中的表达情况。结果卵蛋白诱导法可以成功诱导兔的关节炎,模型组外周血中的 IL-1、TNF-α含量较正常对照组升高（P＜0．05）;三氧化二砷各剂量组兔的关节炎症状有不同程度改善,IL-1、TNF-α含量较模型组降低（P＜0．05）;免疫组化结果显示,模型组关节滑膜的NF-κB（p65）表达较正常组增强（P＜0．05）,而三氧化二砷各剂量组关节滑膜的NF-κB（p65）表达随砷剂剂量的增加表达减弱,但仍然强于正常对照组（P＜0．05）。结论三氧化二砷对实验性类风湿关节炎日本大耳白兔模型有治疗作用。其机制可能与抑制了滑膜细胞中NF-κB（p65）活性和表达,降低了炎性因子IL-1、TNF-α的水平有关。