磁场处理蕃茄种子对幼苗CAT、POD酶活性影响

磁场处理蕃茄种子,观察种子的发芽率幼苗的过氧化氢酶（ＣＡＴ）和过氧化物酶（ＰＯＤ）活性的变化,结果,磁场强度在１０００～２０００ＧＳ范围内,种子发芽率最高,幼苗的过氧化氢酶的过氧化物酶的活性也最高,磁场强度高于３０００ＧＳ以上时,种子发芽率和幼苗酶活性随之降低。     

^60Co—r射线辐射番茄种子对其生理生化影响的研究

用＾６０Ｃｏ－ｒ射线辐射番茄种子,观察辐射种子发芽率、根尖细胞有丝分裂、减数分裂及过氧化物酶同工酶的影响。实验结果表明：辐射剂量１０－２０ｋｒａｄ范围内,种子发芽率最高,有丝分裂畸变率最高,畸变类型最丰富,对减数分裂的抑制小,过氧化物酶同工酶活性最高。     

60Co─r射线辐射番茄种子对其生理生化影响的研究

用６０Ｃｏｒ射线辐射番茄种子,观察辐射种子发芽率、根尖细胞有丝分裂、减数分裂及过氧化物酶同工酶的影响。实验结果表明：辐射剂量１０～２０ｋｒａｄ范围内,种子发芽率最高,有丝分裂畸变率最高,畸变类型最丰富,对减数分裂的抑制小,过氧化物酶同工酶活性最高。     

骆驼蓬提取物浸种对小麦幼苗生长及抗氧化酶活性的影响

以不同浓度骆驼蓬提取液浸种处理小麦。研究对幼苗生长及抗氧化酶活性的影响。结果表明,骆驼蓬提取液浸种后小麦幼苗的根长、株高和干重增加,根冠比增大;幼苗根系活力增强,根系超氧化物歧化酶（SOD）活性提高,过氧化氢酶（CAT）活性先升后降,过氧化物酶（POD）活性下降,过氧化物酶同工酶表达受抑;叶片叶绿素和可溶性蛋白质含量增加,叶片SOD、POD活性提高,过氧化物酶同工酶表达增强,CAT活性降低。根系和叶片丙二醛（MDA）含量下降。     

NaCl胁迫对螺旋藻生长及抗氧化酶活性的影响

在０１％～５．０％ＮａＣｌ浓度范围的培养基中培养极大螺旋藻（Ｓｐｉｒｕｌｉｎａｍａｘｉｍａ）,发现ＮａＣｌ浓度高于２．０％时螺旋藻生长受到明显抑制。培养７天后测定超氧化物歧化酶（ＳＯＤ）、抗坏血酸过氧化物酶（ＡＳＡＰＯＤ）、过氧化氢酶（ＣＡＴ）活性和丙二醛（ＭＤＡ）含量。结果表明：在盐胁迫下,ＳＯＤ酶活性升高;抗坏血酸过氧化物酶和过氧化氢酶活性在低盐胁迫下活性升高,高盐胁迫下抗坏血酸过氧化物酶活性迅速降低,过氧化物酶则完全失活;ＭＤＡ含量先随盐胁迫程度增加而降低,后随盐胁迫的进一步增强恢复至对照水平。     

不同成熟度对水稻种子萌发及其生理特性的影响

对抽穗后15、20、25、30和35d不同成熟度的水稻种子的萌发情况及其生理特性进行了研究。结果表明,随着种子成熟度增加,种子的发芽率、发芽势、活力指数逐渐提高,种子中过氧化物酶（POD）、过氧化氢酶（CAT）、脱氢酶和淀粉酶的活性、可溶性蛋白质和脱落酸（ABA）含量呈现上升趋势,抽穗后30d达最大值,稍后有所下降,而种子的可溶性糖、赤酶素（GA3）和生长素（IAA）含量则呈现下降趋势。在抽穗后15～35d,‘996’种子的发芽率、发芽势、活力指数、4种酶活性、可溶性蛋白质含量、可溶性糖含量、GA3和IAA含量均高于‘4628’种子的,而ABA含量则低于‘4628’种子。     

白内障晶体中某些微量元素,与谷胱甘肽相关酶类活性的动态观察…

本文动态观察了用平阳霉素诱发的大鼠白内障晶体中与谷胱甘肽代谢相关酶类活性和微量元素水平的变化,并与正常晶体进行比较,同时就酶活性与微量元素水平的相关性进行了检验。结果表明：（１）注射平阳霉素早期酶活性增高,谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）、谷胱甘肽还原酶（ＧＳＳＧ－Ｒ）及超氧化物歧化酶（ＳＯＤ）等活性的升高达显著水平,后期酶活性均下降,尤以ＧＳＨ－Ｐｘ、ＧＳＳＧ－Ｒ和谷胱甘肽硫转移酶（ＧＳＨ－Ｓ     

高压静电场（HVEF）对大豆种子吸胀冷害的影响

高压静电场预处理可不同程度地提高大豆种子抗低温吸胀冷害的能力,处理组种子在低温吸胀时无机离子,可溶性糖和可溶性蛋白的外渗量显著低于对照组,静电场预处理还提高了种子中过氧化氢酶（简ＣＡＴ）、过氧化物酶（简ＰＯＤ）、超氧物歧化酶（简ＳＯＤ）等清除自由基的酶活性,降低了丙二醛（简ＭＤＡ）的积累,促进了种子代谢水平,即提高了种子的淀粉酶、脱氢酶的活力和可溶性蛋白含量,最终提高了发芽率。本文以膜的相变和损伤     

白内障晶体中某些微量元素、与谷胱甘肽相关酶类活性的动态观察及其相互关系

本文动态观察了用平阳霉素诱发的大鼠白内障晶体中与谷胱甘肽代谢相关酶类活性和微量元素水平的变化,并与正常晶体进行比较,同时就酶活性与微量元素水平的相关性进行了检验。结果表明：（１）注射平阳霉素早期酶活性增高,谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）、谷胱甘肽还原酶（ＧＳＳＧ－Ｒ）及超氧化物歧化酶（ＳＯＤ）等活性的升高达显著水平,后期酶活性均下降,尤以ＧＳＨ－Ｐｘ、ＧＳＳＧ－Ｒ和谷胱甘肽硫转移酶（ＧＳＨ－Ｓ）等的活性降低明显;（２）ＧＳＨ－Ｐｘ和ＳＯＤ酶活性分别与Ｚｎ具有相关性（Ｐ＜０．０５）,这两种酶也分别与Ｓｅ具有高度相关性（Ｐ＜０．０１）,此两种元素在该类型白内障形成中可能有一定意义。     

场效应对作物萌发期酶活性动态影响

小麦１５０ＧＹ，玉米５０ＧＹ剂量γ场处理的种子，在萌发期超氧化物歧化酶，淀粉酶活性峰值出现期提前，低磁（６５０ＧＳ）高磁（１３５０ＧＳ）处理的种子，提前α－淀粉酶活性峰值及总淀粉酶活性第一次峰值出现的时间。１．２小时微米波处理处理的种子，在萌发期超氧化物歧化酶二次活性峰值出现期提前α－淀粉酶活性出现双峰值，总淀粉酶两次活性峰值出现时间均提前，低剂量γ场，恒定磁场，微米波处理的种子发芽势，发芽率，百     

植物向重性地基研究

植物向重性的机理研究与植物在微重力环境下的生长发育有关。本文在地基１ ×ｇ 重力场上进行了幼苗重力反应实验,结果表明：重力引起水平放置的幼苗过氧化物酶在根尖组织上、下侧的差异分布;在重力场上水平放置的幼苗其根尖向高钙（Ｃａ２＋） 一侧弯曲生长;当给倒置幼苗的顶端照光时其负向地性消失。     

The role of peroxidases in pistil-pollen interactions

Summary The majority of pistil peroxidases are involved in processes related to growth, development and senescence. Only the tissue specific peroxidases in the transmitting tissue of the style may play a direct role in the regulation of pollen tube growth. The pollen peroxidases may function mainly in growth regulation and tube wall formation and play a role in the interaction between pollen and pistil by metabolizing the phenolic compounds in the pistil.     

荞麦愈伤组织分化与过氧化物酶活性及其同工酶关系的研究

在附加不同外源激素的培养基中,龙山荞的子叶愈伤组织表现出不同的分化状态:(1)不分化;(2)分化根;(3)分化芽;(4)分化全苗。四种愈伤组织的过氧化物酶活性的相对比值为1:1.47:5.95:7.78,并且过氧化物酶同工酶谱也存在明显差异。酶带4'为分化愈伤组织所特有;酶带2'为未分化和分化愈伤组织所共有;在分化根和未分化愈伤组织中缺少酶带1';未分化愈伤组织比分化愈伤组织多出酶带3'。     

Die Lokalisation endogener Peroxydase in der Glandula parotis der Ratte

Zusammenfassung Die Lokalisation endogener Peroxydase wurde in der Glandula parotis der Ratte mit der Methode von Graham und Karnovsky (1966) untersucht. Lichtmikroskopisch ist das Reaktionsprodukt im basalen Cytoplasma, in den Sekretgranula und, nach Pilocarpinreizung, in den Lumina der interzellulären Sekretkapillaren, der Drüsenendstücke und der Schaltstücke nachweisbar. Der Speichel enthält eine Peroxydase, die durch Hitze (100°) und durch KCN und 3-Amino-1,2,4-Triazol hemmbar ist. Der Speichel zeigt bei 415 m eine Schulter im Absorptionsspectrum, die nach Komplexbildung mit H₂O₂ um 15 m nach rechts verschoben wird. Elektronenmikroskopisoh läßt sich das Reaktionsprodukt der Peroxydase in allen Cisternen des rauhen endoplasmatischen Reticulums, einschließlich der perinucleären Cisterne, in glattwandigen Bläschen zwischen rauhem endoplasmatischen Reticulum und Golgi-Membranstapeln, in den kondensierenden Vacuolen und in allen Sekretgranula nachweisen. Die Cisternen des Golgi-Apparates enthalten selten Reaktionsprodukt. In der Glandula parotis der Ratte sind vorwiegend die kondensierenden Vacuolen, in geringerem Maße auch die Cisternen des Golgi-Apparates, an der Segregierung und Kondensierung von Peroxydase beteiligt.

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The localization of endogenous peroxidase in the parotid gland of the rat

Summary The localization of endogenous peroxidase was investigated in the parotid gland of the rat by the method of Graham and Karnovsky (1966). By light microscopy, the reaction product was localized in the basal cytoplasm, in secretion granules, and, after injection of pilocarpine hydrochloride, in the lumina of the intercellular canaliculi, the acini and the intercalated ducts. The peroxidase reaction of the saliva collected from the oral cavity can be suppressed by heat (100° C), by 3-amino-1,2,4-triazole, and by KCN. Absorption spectra of the saliva show a shoulder at 415 m which is shifted by 15 m towards longer wave lengths after complexing with H₂O₂. At the ultrastructural level, reaction product is present in all cisternae of the rough endoplasmic reticulum including the perinuclear cisternae, in smooth vesicles located between the rough endoplasmic reticulum and the stacks of Golgi cisternae, in condensing vacuoles, and in all secretion granules. The Golgi cisternae seldom contain reaction product. The results show that in the parotid gland of the rat, the condensing vacuoles and, to a lesser extent, the cisternae of the Golgi apparatus function in the segregation and condensation of peroxidase.

Nachtrag bei der Korrektur: In einer soeben veröffentlichten Arbeit (Strum und Karnovsky, J. Ultrastructure Res. 31, 323–336, 1970) wurde endogene Peroxydase im endoplasmatischen Reticulum, in den membrangebundenen Ribosomen und gelegentlich in den Sekretgranula der Glandula submaxillaris der Ratte nachgewiesen.     

Neutrophil-Catalysed Dimerisation of Tyrosyl Peptides

Evidence is given that tyrosyl-peptides are dimerised by polymorphonuclear leukocytes leading to a new family of compounds. The products formed are homo- and hetero-dimeric peptides with linkage between the tyrosyl residues. This corresponds to a dityrosine structure as determined by analytic and spectroscopic data.     

Inertness of horseradish peroxidase to 2,4-dichlorophenoxyacetic acid

The possibility of mutual effects of 2,4-D and horseradish (Armoracia lapathifolia L.) peroxidase on each other has been explored by four procedures. (i) Compounds I, II, and III of horseradish peroxidase (HRP) and H₂O₂ were exposed to 2,4-D. (ii) Extracts from batchwise operations of HRP + H₂O₂ and 2,4-D were analyzed for oxidation products by means of thin layer chromatography. (iii) The velocity of the IAA oxidase reaction with HRP as catalyst, and (iv) K_m and V_s of the overall peroxidation of guaiacol by HRP + H₂O₂, were determined in the absence and presence of 2,4-D. The results failed to show any effect of 2,4-D; only at very high concentrations did 2,4-D slightly inhibit the oxidation of IAA by one isoperoxidase. It is concluded that 2,4-D does not promote growth in plants by hampering a peroxidase-catalyzed IAA oxidation. It seems probable that 2,4-D perturbs the isoperoxidase pattern by acting at some step prior to the release of the enzyme from its site of synthesis.     

Characterization of peroxidase:anti-peroxidase immune complexes by capillary zone electrophoresis and high-performance size-exclusion chromatography

Determination of the molecular constituents of commercial peroxidase:anti-peroxidase (PAP) preparations is necessary for the proper interpretation of PAP applications based on competitive binding assay. Capillary zone electrophoresis with field 300 V/cm, 40 cm capillary length (20 cm effective length), and high-performance size exclusion chromatography equipped with Superose12 HR10/30 column revealed that a PAP preparation used for Fcγ receptor studies contained multiple sizes of immune complexes, an excess amount of free peroxidase, and little or no free anti-peroxidase antibody. The antibody:antigen ratios of the three major immune complex components were 2:2, 1:2, and 1:1. These techniques provide useful methods of qualitative, as well as quantitative analysis of PAP preparations.     

黄瓜子叶外植体龄对子叶生根的影响

本文对无菌培养条件下黄瓜子叶外植体龄对子叶生根能力的影响进行了研究,结果表明,对于分别萌发５天、１０天、１５天、２０天的子叶在ＭＳ基本培养基上,无论有生长系ＮＡＡ,均以５天龄的子叶生根能力最强。附加１．０ｍｇ／ＬＮＡＡ,显著促进子叶生根。对子叶起始过氧化物酶活性测定表明,子叶起始过氧化物酶活性与生根能力之间存在有一定的关系。     

Multidomain Human Peroxidasin 1 Is a Highly Glycosylated and Stable Homotrimeric High Spin Ferric Peroxidase

Human peroxidasin 1 (hsPxd01) is a multidomain heme peroxidase that uses bromide as a cofactor for the formation of sulfilimine cross-links. The latter confers critical structural reinforcement to collagen IV scaffolds. Here, hsPxd01 and various truncated variants lacking nonenzymatic domains were recombinantly expressed in HEK cell lines. The N-glycosylation site occupancy and disulfide pattern, the oligomeric structure, and unfolding pathway are reported. The homotrimeric iron protein contains a covalently bound ferric high spin heme per subunit with a standard reduction potential of the Fe(III)/Fe(II) couple of −233 ± 5 mV at pH 7.0. Despite sequence homology at the active site and biophysical properties similar to human peroxidases, the catalytic efficiency of bromide oxidation (k_cat/K_M^app) of full-length hsPxd01 is rather low but increased upon truncation. This is discussed with respect to its structure and proposed biosynthetic function in collagen IV cross-linking.