共查询到20条相似文献，搜索用时 31 毫秒
1.
Emilio A. Martinez Cristina A. Martinez Josep M. Cambra Carolina Maside Xiomara Lucas Jose L. Vazquez Juan Maria Vazquez Jordi Roca Heriberto Rodriguez‐Martinez Maria Antonia Gil Inmaculada Parrilla Cristina Cuello 《Reproduction in domestic animals》2019,54(Z4):4-13
Commercial embryo transfer (ET) has unprecedented productive and economic implications for the pig sector. However, pig ET has been considered utopian for decades mainly because of the requirements of surgical techniques for embryo collection and embryo deposition into recipients, alongside challenges to preserve embryos. This situation has drastically changed in the last decade since the current technology allows non‐surgical ET and short‐ and long‐term embryo preservation. Here, we provide a brief review of the improvements in porcine ET achieved by our laboratory in the past 20 years. This review includes several aspects of non‐surgical ET technology and different issues affecting ET programmes and embryo preservation systems. The future perspectives of ET technology are also considered. We will refer only to embryos produced in vivo since they are the only type of embryos with possible short‐term use in pig production. 相似文献
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澳洲波尔山羊胚胎3种冷冻方法对其胚胎移植效果的影响 总被引：2，自引：0，他引：2
在25℃室温下,采用细管法(一步法、二步法)和OPS法,以不同浓度的EFS、EDFS为玻璃化冷冻液,对澳洲波尔山羊致密桑椹胚和囊胚进行玻璃化冷冻保存。同时利用1.5mol/LEG为抗冻保护剂对胚胎进行常规法冷冻保存。分别将上述3种方法冷冻解冻后的胚胎移植于同期发情后6~7d的云南黑山羊受体。结果表明,细管法胚胎玻璃化冷冻保存效果均以EFS40组为佳,解冻后胚胎移植产羔率分别为40.54%(15/37;一步法)和51.35%(19/37;二步法)。与新鲜胚胎移植产羔率(52.50%,21/40)和常规法冷冻保存的胚胎移植产羔率(45.16%,14/31)相比无显著性差异(P>0.05)。另外,用EDFS30玻璃化溶液,OPS法冷冻解冻后的胚胎移植产羔率高达51.43%(18/35),为整个玻璃化冷冻试验的最佳值。玻璃化冷冻方法简便、迅速,无论是细管法还是OPS法均获得了比较理想的胚胎移植效果。 相似文献
3.
M. L. H. Campbell 《Equine Veterinary Education》2014,26(6):322-327
Embryo transfer (ET) is an accepted and successful technique for obtaining foals from mares without interrupting their competition careers. Recent research, however, suggests that the potential of factors including heat, exercise, repeated embryo flushing and repeated manipulation of the reproductive cycle using exogenous hormones to have a negative impact on fertility may have been underestimated. This paper reviews the evidence base for involvement of these factors in repeated failures to recover embryos from nongeriatric competition mares without obvious clinical or pathological indications of reproductive abnormalities. It concludes that, for some mares at least, a cessation of exercise for the periovulatory period and the period between ovulation and embryo flushing, combined with careful management of flushing‐induced endometritis, and minimal hormonal manipulation of the reproductive cycle, may be necessary to optimise embryo recovery rates. Mare owners may have been encouraged to request ET for their mares following high‐profile examples in the media of elite mares that have produced foals by ET whilst competing. The veterinarian should educate mare owners about the multiple factors that may affect the chances of recovering an embryo from their mares, and should manage the expectations of mare owners so that they do not approach ET programmes in the expectation that there will be no disruption to their training and competition plans. 相似文献
4.
牛体细胞核移植胚胎的批量化生产 总被引：8，自引：0，他引：8
利用2枚显微去核后的半卵与1枚供体细胞同步融合,并结合微穴法(WOWs)培养体系批量化生产成年牛克隆胚胎(日产40～80枚).结果,重构胚电融合率95.7%(3059/3197)、卵裂率87.1%(2637/3027)、囊胚率41.1%(1244/3027)和可冻胚率(72.5%,933/1244)均达到较高水平.克隆胚胎采用玻璃微管玻璃化冷冻保存数月后移植,30日龄时妊娠率为28.1%(48/171),已经产下5头足月克隆牛续.结果表明,该方法具有产业开发潜力. 相似文献
5.
The present study was conducted to evaluate the possibility of using in vitro‐produced parthenogenetic (PA) embryos for co‐transfer with morulae that had been collected in vivo and cryopreserved. The proportion of PA blastocysts (20.5%) was higher than that of their in vitro fertilization (IVF) counterparts (16.6%). Although there were no differences in morphology or diameter between the two groups, the number of cells in early PA blastocysts after in vitro culture for 6 days was lower than for IVF blastocysts (25.7 and 30.4 cells, respectively), and the number in recovered PA blastocysts was also smaller than that in recovered IVF blastocysts (37.4 and 50.2 cells, respectively). When 10 morulae warmed after vitrification were co‐transferred with 10 PA blastocysts (total 20 embryos) to the uterus of five recipients, the rates of pregnancy and farrowing did not differ, but the average period until spontaneous abortion tended to be longer relative to the control (when 20 morulae were transferred). These data suggest that in vitro‐produced PA embryos offer the possibility of assisted pregnancy for cryopreserved embryos; further experiments will be needed to confirm the beneficial effect of this approach on piglet production. 相似文献
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Yuri Hirayama Rie Takishita Hiroyasu Misawa Kazuhiro Kikuchi Koji Misumi Sachiko Egawa Sawako Motoyama Yasunobu Hasuta Yoshiyuki Nakamura Yutaka Hashiyada 《Animal Science Journal》2020,91(1)
This study aimed to compare the efficiency of non‐surgical embryo transfer (ET) using a newly developed catheter, which enables transferring embryos into a proximal site of the uterus (mostly uterine body), and surgical ET of vitrified porcine embryos. In Experiment 1, the catheter was inserted into 12 gilts, with each half of the group allocated to skilled or novice operators. The time required for insertion into the uterus did not differ between skilled and novice operators (4 min 9 s and 4 min 6 s, respectively). In Experiment 2, 12 gilts were used as recipients for non‐surgical and surgical ET with vitrified embryos (n = 6, each). There was no significant difference in the rate of piglet production based on the number of transferred embryos between surgical and non‐surgical ET (25.8% vs. 15.4%, p = .098). The results suggest that non‐surgical ET catheter allowed for easy insertion and transfer of embryos without special training. Although the catheter is effective for deposition of embryos into the proximal site of uterus, the efficiency of piglet production is not enhanced compared with surgical ET. The ET method using this catheter, being labor‐saving and less‐invasive, may contribute to the improvement of ET in pigs. 相似文献
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哺乳动物胚胎冷冻技术研究进展 总被引：2，自引：0，他引：2
胚胎冷冻保存已广泛用于胚胎移植，动物克隆以及动物资源保护。此技术的应用需保证胚胎在冷冻一解冻后具有较高的成活率。自1972年小鼠胚胎冷冻获得成功以来，许多学者在简化冷冻程序，缩短冷冻时间等方面进行了深入研究，试图寻找一种可用于生产的冷冻方法。本文全面地比较了不同的冷冻方法及其结果，籍此为该领域工作者提供一定帮助。 相似文献
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Kiyoshi AKIYAMA Jun KOBAYASHI Yoshimasa SATO Ryuichi SATA Megumi OHASHI Emi SASAKI Yorimasa ODA Yoshio OGAWA Shuji UEDA Hisashi NABENISHI Satoko MATOBA 《Animal Science Journal》2010,81(4):461-466
The objective of this study was to develop an in‐straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified‐warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in‐straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in‐straw dilution (Non‐evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non‐evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in‐straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in‐straw method and that the vitrified and warmed sexed embryos can develop to term. 相似文献
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Background Induction of multiple ovulations, or superovulation, may potentially increase the efficiency of equine embryo transfer programs. Our objective was to investigate the effects of equine follicle‐stimulating hormone (eFSH) treatment on the success rate of embryo transfer programs in mares. Methods In the research facility of the University of Saskatchewan, Canada, we studied 12 donor mares and 37 recipient mares during the physiological breeding season. Donor mares were used in two consecutive oestrous cycles: the first served as the control cycle and in the second an eFSH regimen was applied (eFSH cycle). In the control cycle, mares were administered human chorionic gonadotropin (hCG) to induce ovulation when a follicle ≥35 mm in diameter was detected by transrectal ultrasonographic examination. In the second oestrous cycle, twice‐daily eFSH treatment was initiated when a follicle ≥25 mm was detected and treatment ceased when a follicle ≥35 mm was present, at which time hCG was administered. All donor mares were artificially inseminated while in oestrus using fresh semen collected from a stallion of proven fertility. At 8 days post‐ovulation, embryos were recovered transcervically and transferred individually to the uterus of a synchronised recipient mare. Results The eFSH treatment stimulated the ovary and resulted in greater numbers of ovulations and recovered embryos; however the recovered embryos tended to have a lower morphological grade than the control embryos, and the recipient pregnancy rate per transferred embryo was lower than anticipated. Conclusion The numbers of recipient pregnancies and foals born that resulted from eFSH treatment were not different from the control. 相似文献
13.
Tayita Suttirojpattana Theesit Juanpanich Rangsun Parnpai Teraporn Vutyavanich 《Animal Science Journal》2021,92(1):e13585
Two-cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi-straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5-ml straw. In two-cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two-cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non-collapsed; HFV-NC and collapsed; HFV-C blastocyst). Re-expansion rate in 15% HFV-NC, 17.5% HFV-NC, and 15% HFV-C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi-straw, which does not require skilled practitioners. 相似文献
14.
牛胚胎冷冻保存的研究进展 总被引：6，自引：0，他引：6
目前,牛的胚胎冷冻保存巳成为一种较成熟的常规技术,广泛应用于牛的繁殖科研与生产。本文回顾了牛胚冷冻保存技术的发展概况,对牛胚胎的常规冷冻技术、直接冷冻法及玻璃化冷冻技术的进展作了简要论述。 相似文献
15.
Luis B. Ferré Michael E. Kjelland Ahmed M. Taiyeb Fernando Campos-Chillon Pablo J. Ross 《Reproduction in domestic animals》2020,55(6):659-676
Cryopreservation of in vitro-derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high-merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra-low temperature storage method in order to maximize donor ovum pickup (OPU) turn-over, recipient availability/utilization and domestic/overseas commercial trading opportunities. However, IVP bovine embryos are still very sensitive to chilling and cryopreservation, and despite recent progress, a convenient (simple and robust) protocol has not yet been developed. At the moment, there are two methods for bovine IVP embryo cryopreservation: slow programmable freezing and vitrification. Both of the aforementioned techniques have pros and cons. While controlled-rate slow cooling can easily be adapted for direct transfer (DT), ice crystal formation remains an issue. On the other hand, vitrification solved this problem but the possibility of successful DT commercial incorporation remains to be determined. Moreover, simplification of the vitrification protocol (including warming) through the use of an in-straw dilution without the use of a microscope is a prerequisite for its use under farm conditions. This review summarizes the bovine IVP embryo cryopreservation achievements, strengths and limitations of both freezing systems and prospective improvements to enhance cryosurvival, as well as perspectives on future directions of this assisted reproductive technology. 相似文献
16.
A.I. Moore MS PhD E.L. Squires PhD J.E. Bruemmer MS PhD J.K. Graham PhD 《Journal of Equine Veterinary Science》2006,26(5):215-218
Cryosurvival of cells is reduced if the cooling rate used is suboptimal. If cells cool too rapidly, intracellular water will freeze, causing intracellular ice crystals. However, if spermatozoa are cooled too slowly, excessive cellular dehydration occurs, causing irreversible damage to cellular compartments. In addition, cryoprotectants are added to the freezing diluent to protect cells from damage during cryopreservation. This study was conducted to determine the optimal cooling rate for stallion spermatozoa frozen in the presence of three different cryoprotectants. Spermatozoa were frozen in a skim milk, egg yolk diluent containing 4% glycerol, and ethylene glycol or dimethyl formamide at 10 different cooling rates ranging from 5°C/min to 50°C/min. The percentage of viable spermatozoa was higher for spermatozoa cooled at 10°C/min than at 50°C/min (P < .05). Spermatozoa frozen using glycerol as the cryoprotectant had higher percentages of motile and progressively motile spermatozoa compared with spermatozoa frozen using the other two cryoprotectants (P < .05). In conclusion, the cryosurvival of stallion spermatozoa is similar when cooling rates of 5°C/min to 45°C/min are used, and when 4% cryoprotectant is used, glycerol is a more effective cryoprotectant than ethylene glycol or dimethyl formamide. 相似文献
17.
探讨程序化冷冻与玻璃化冷冻对小鼠GV期卵母细胞及二细胞期胚胎的复苏率及其发育潜能的影响。通过小鼠的卵母细胞与早期胚胎的不同冷冻方法的比较,为后续阿旺绵羊的胚胎冷冻保存提供参考。采用程序化冷冻与玻璃化冷冻技术,分别冷冻小鼠GV期卵母细胞及二细胞期胚胎,复苏后培养,比较不同冷冻处理后的复苏率、成熟率与囊胚率。小鼠GV期卵母细胞程序化冷冻复苏率(48.00%±5.29%)显著低于玻璃化冷冻复苏率(65.00%±5.00%),有统计学差异(P=0.0147<0.05);而程序化冷冻后复苏卵母细胞的发育成熟率略高于玻璃化冷冻组,但无统计学意义。小鼠二细胞期胚胎程序化冷冻组复苏率(76.00%±2.00%)显著高于玻璃化冷冻组复苏率(70.00%±2.00%),有统计学差异(P=0.0213<0.05);冷冻后复苏胚胎发育的囊胚率程序化冷冻略低于玻璃化冷冻及对照组,但无统计学意义。 相似文献
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在25℃室温和37℃恒温台条件下，利用玻璃化冷冻溶液EFS30、EFS40、EDFS30或EDFS40，对小鼠4-细胞胚胎进行玻璃化冷冻保存．以解冻后培养72h的囊胚发育率为其体外发育能力的考核指标，同时对解冻后培养1～3h的胚胎进行移植以判定其体内发育潜力。开放式拉长塑料细管（OPS）二步法冷冻保存，即胚胎首先移入预处理液（10％EG或10％EG＋10％DMSO）中平衡30s，再移入玻璃化溶液中洗涤后吸入OPS管中，分别经35、30或25s后直接投入液氮中冷冻保存。一步法冷冻保存则无需预处理液处理。结果表明，小鼠胚胎4-细胞一步法和二步法冷冻后囊胚最高发育率分别为87．7％和88.6％，与对照组（93.0％）差异不显著（P〉0．05）。利用最佳冷冻组获得的143枚胚胎移植于12只假妊娠50～60h的受体母鼠，结果有4只妊娠产仔17只，妊娠产仔率为42．5％（17／40），与对照组59．4％（19／32）差并不显著（P〉0.05）。 相似文献
19.
牛体外受精胚的玻璃化保存及细胞遗传学研究 总被引：1，自引：0，他引：1
用25％的乙二醇和0.2mol/L蔗糖与25％忆二醇混合的玻璃化溶液对牛体外受精的早期囊胚用1步法冷漠保存，融解后，在D－PBS液（含有0.5mol蔗糖）中15min除去冷冻保护剂，培养48和96h。结果表明：用含有0.2mol的蔗糖和25％的乙二混合的玻璃化溶液保存的早期囊胚的生态力明显高于不含蔗糖的玻璃化溶液（P＜0.001）。染色体分析结果表明：含有蔗糖的玻璃化溶液保存的囊胚的卵裂细胞数明显高于单独使用25％乙醇保存的囊胚（P＜0.05）。染色体异常发生率无明显变化。 相似文献
20.
Tamas Somfai Van Khanh Nguyen Huong Thu Thi Vu Huong Le Thi Nguyen Huu Xuan Quan Nguyen Viet Linh Son Le Phan Lan Doan Pham Ngo Thi Kim Cuc Kazuhiro Kikuchi 《Animal Science Journal》2019,90(7):840-848
We report the cryopreservation of oocytes from Ban miniature pigs which are endemic in Vietnam. Immature cumulus‐oocyte complexes were collected from antral follicles of 7–8 mo old female cyclic Ban pigs and vitrified in micro‐drops. Oocyte morphology, lipid content, post‐warming survival, nuclear maturation, and embryo development were compared to those of oocytes from commercially slaughtered Landrace × Large white hybrid pigs. The size of oocytes in the two breeds was similar. However, significantly lower amounts of intracellular lipid were detected in Ban oocytes. There was no difference (p > 0.05) between Ban and Landrace × Large white oocytes in percentages of post‐warming survival (93.1 ± 3.4% vs. 70.7 ± 16.7%, respectively) and nuclear maturation after in vitro maturation (80.4 ± 5.1% vs. 90.0 ± 1.3% respectively). Similarly, cleavage (30.8 ± 7.8% vs. 10.3 ± 6.1%, respectively) and blastocyst development rates (9.4 ± 5.0% vs. 0.79 ± 0.79, respectively) were not different (p > 0.05) between vitrified Ban and Landrace × Large white oocytes after in vitro fertilization and embryo culture. In conclusion, high survival and maturation rates were achieved after vitrification of immature Ban oocytes and their cryo‐tolerance was similar to that of Landrace × Large white oocytes, despite the difference in lipid content. We succeeded to generate reasonable rates of blastocysts from vitrified Ban oocytes by in vitro fertilization. 相似文献
